LOCUS       Exported                6308 bp ds-DNA     circular SYN 23-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    56..222
                     /label=ITR
                     /note="ITR"
     misc_feature    239..243
                     /label=Destroyed BglII and PstI
                     /note="Destroyed BglII and PstI"
     misc_feature    244..709
                     /label=synapsin promoter
                     /note="synapsin promoter"
     misc_feature    710
                     /label=Killed EcoRI site (GAATTC > CAATTC)
                     /note="Killed EcoRI site (GAATTC > CAATTC)"
     misc_feature    742..804
                     /label=Ig K-Chain
                     /note="Ig K-Chain"
     misc_feature    805..810
                     /label=MCS
                     /note="MCS"
     misc_feature    811..1635
                     /label=Pf622
                     /note="Pf622"
     misc_feature    1108..1110
                     /label=F101L
                     /note="F101L"
     misc_feature    1585..1587
                     /label=N260A
                     /note="N260A"
     misc_feature    1636..1911
                     /label=cpSFGFP
                     /note="cpSFGFP"
     misc_feature    1636..1641
                     /label=L1-SH>AN
                     /note="L1-SH>AN"
     misc_feature    1642..1644
                     /label=GFP-N149Q
                     /note="GFP-N149Q"
     misc_feature    1648..1650
                     /label=GFP-Y151R
                     /note="GFP-Y151R"
     misc_feature    1654..1656
                     /label=M153T
                     /note="M153T"
     misc_feature    1681..1683
                     /label=K162M
                     /note="K162M"
     misc_feature    1912..1929
                     /label=Linker
                     /note="Linker"
     misc_feature    1930..2367
                     /label=cpSFGFP
                     /note="cpSFGFP"
     misc_feature    2362..2364
                     /label=F145W
                     /note="F145W"
     misc_feature    2368..2487
                     /label=Pf622
                     /note="Pf622"
     misc_feature    2368..2373
                     /label=L2-AN
                     /note="L2-AN"
     misc_feature    2500..2532
                     /label=Myc epi
                     /note="Myc epi"
     misc_feature    2533..2682
                     /label=PDGFR 513-561
                     /note="PDGFR 513-561"
     misc_feature    2695..3283
                     /label=WPRE
                     /note="WPRE"
     misc_feature    2831..2851
                     /label=Mao 115r
                     /note="Mao 115r"
     misc_feature    3317..3548
                     /label=SV40
                     /note="SV40"
     misc_feature    3577..3743
                     /label=ITR
                     /note="ITR"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc
       61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
      121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
      181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag
      241 atccagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg
      301 gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa
      361 ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac
      421 tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc
      481 gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg
      541 gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg
      601 agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc
      661 agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcc aattcaagct
      721 gctagcaagg atcccgccac catggagaca gacacactcc tgctatgggt actgctgctc
      781 tgggttccag gttccactgg tgacagatct gaaagcatca atttcgtgag ctggggcggt
      841 agcacccagg atgcgcagaa gcaggcctgg gccgacccgt tcagcaaggc cagcggcatt
      901 accgtggtcc aggatgggcc caccgactac ggcaaactca aggccatggt cgaaagcggc
      961 aacgtgcagt gggacgtggt ggatgtagag gccgacttcg ccttgcgcgc cgccgccgaa
     1021 ggcctgctcg aacccctgga tttctcggtg atccagcgcg acaagatcga cccgcgcttc
     1081 gtctccgacc atggcgtggg ctcgttcctg ttctctttcg tgctcggcta caacgaaggc
     1141 aagctcggag ccagcaagcc ccaggactgg accgcgctgt tcgacaccaa gacctacccc
     1201 ggcaaacgcg ccctctacaa atggccgagc cctggcgtgc tcgaactggc cctgctcgcc
     1261 gacggcgtac cggccgacaa gctctacccg ctggacctgg accgcgcctt caagaaactc
     1321 gacaccatca agaaagacat cgtctggtgg ggcggcggtg cacagtcgca gcagctgctg
     1381 gcctccggcg aagtcagcat gggccagttc tggaacggtc gcatccacgc cctgcaagaa
     1441 gacggcgcgc cagtgggcgt gagctggaag cagaacctgg tgatggccga catcctcgtc
     1501 gtgcctaaag gcacgaagaa caaagccgcc gcgatgaagt tcctggccag tgccagcagc
     1561 gccaaaggcc aggacgactt ttccgccctg accgcctatg ctccagtgaa catcgacagt
     1621 gtgcagcgcc tcgaccttgc gcaggtccgg atcaccgccg acaagcagaa gaacggcatc
     1681 atggcgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct cgccgaccac
     1741 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1801 agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1861 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1921 ggagggagca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1981 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     2041 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2101 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2161 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2221 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     2281 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2341 gggcacaagc tggagtacaa ctggaacgcc aacctggcac cgaacctgcc gaccgcctac
     2401 gtcaaggatc agatcaccct cgatttcgcc tactgggcca agaacggtcc ggccattgcg
     2461 acacggtgga atgaatggct agtcaaactg caggtcgacg aacaaaaact catctcagaa
     2521 gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg
     2581 ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc
     2641 cttatcatcc tcatcatgct ttggcagaag aagccacgtt agaagcttat cgataatcaa
     2701 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2761 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2821 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2881 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2941 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3001 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3061 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3121 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3181 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3241 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     3301 cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     3361 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3421 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3481 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     3541 ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt
     3601 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3661 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     3721 ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca
     3781 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3841 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3901 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4261 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4321 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4501 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4561 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4621 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4681 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4741 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4801 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4861 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4921 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4981 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5041 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5101 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5161 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5221 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5281 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5341 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5401 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5461 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6181 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6241 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6301 gcggaaga
//
