LOCUS Exported 6308 bp ds-DNA circular SYN 23-MAY-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6308) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6308 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 56..222 /label=ITR /note="ITR" misc_feature 239..243 /label=Destroyed BglII and PstI /note="Destroyed BglII and PstI" misc_feature 244..709 /label=synapsin promoter /note="synapsin promoter" misc_feature 710 /label=Killed EcoRI site (GAATTC > CAATTC) /note="Killed EcoRI site (GAATTC > CAATTC)" misc_feature 742..804 /label=Ig K-Chain /note="Ig K-Chain" misc_feature 805..810 /label=MCS /note="MCS" misc_feature 811..1635 /label=Pf622 /note="Pf622" misc_feature 1108..1110 /label=F101L /note="F101L" misc_feature 1111..1113 /label=F102Y /note="F102Y" misc_feature 1216..1218 /label=Y137L /note="Y137L" misc_feature 1585..1587 /label=N260A /note="N260A" misc_feature 1636..1911 /label=cpSFGFP /note="cpSFGFP" misc_feature 1636..1641 /label=L1-SH>AN /note="L1-SH>AN" misc_feature 1642..1644 /label=GFP-N149Q /note="GFP-N149Q" misc_feature 1648..1650 /label=GFP-Y151R /note="GFP-Y151R" misc_feature 1654..1656 /label=M153T /note="M153T" misc_feature 1681..1683 /label=K162M /note="K162M" misc_feature 1912..1929 /label=Linker /note="Linker" misc_feature 1930..2367 /label=cpSFGFP /note="cpSFGFP" misc_feature 2362..2364 /label=F145W /note="F145W" misc_feature 2368..2487 /label=Pf622 /note="Pf622" misc_feature 2368..2373 /label=L2-AN /note="L2-AN" misc_feature 2500..2532 /label=Myc epi /note="Myc epi" misc_feature 2533..2682 /label=PDGFR 513-561 /note="PDGFR 513-561" misc_feature 2695..3283 /label=WPRE /note="WPRE" misc_feature 2831..2851 /label=Mao 115r /note="Mao 115r" misc_feature 3317..3548 /label=SV40 /note="SV40" misc_feature 3577..3743 /label=ITR /note="ITR" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc 61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc 121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt 181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag 241 atccagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg 301 gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa 361 ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac 421 tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc 481 gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg 541 gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg 601 agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc 661 agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcc aattcaagct 721 gctagcaagg atcccgccac catggagaca gacacactcc tgctatgggt actgctgctc 781 tgggttccag gttccactgg tgacagatct gaaagcatca atttcgtgag ctggggcggt 841 agcacccagg atgcgcagaa gcaggcctgg gccgacccgt tcagcaaggc cagcggcatt 901 accgtggtcc aggatgggcc caccgactac ggcaaactca aggccatggt cgaaagcggc 961 aacgtgcagt gggacgtggt ggatgtagag gccgacttcg ccttgcgcgc cgccgccgaa 1021 ggcctgctcg aacccctgga tttctcggtg atccagcgcg acaagatcga cccgcgcttc 1081 gtctccgacc atggcgtggg ctcgttcctg tattctttcg tgctcggcta caacgaaggc 1141 aagctcggag ccagcaagcc ccaggactgg accgcgctgt tcgacaccaa gacctacccc 1201 ggcaaacgcg ccctcctaaa atggccgagc cctggcgtgc tcgaactggc cctgctcgcc 1261 gacggcgtac cggccgacaa gctctacccg ctggacctgg accgcgcctt caagaaactc 1321 gacaccatca agaaagacat cgtctggtgg ggcggcggtg cacagtcgca gcagctgctg 1381 gcctccggcg aagtcagcat gggccagttc tggaacggtc gcatccacgc cctgcaagaa 1441 gacggcgcgc cagtgggcgt gagctggaag cagaacctgg tgatggccga catcctcgtc 1501 gtgcctaaag gcacgaagaa caaagccgcc gcgatgaagt tcctggccag tgccagcagc 1561 gccaaaggcc aggacgactt ttccgccctg accgcctatg ctccagtgaa catcgacagt 1621 gtgcagcgcc tcgaccttgc gcaggtccgg atcaccgccg acaagcagaa gaacggcatc 1681 atggcgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct cgccgaccac 1741 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 1801 agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 1861 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc 1921 ggagggagca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 1981 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc 2041 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2101 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 2161 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2221 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac 2281 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2341 gggcacaagc tggagtacaa ctggaacgcc aacctggcac cgaacctgcc gaccgcctac 2401 gtcaaggatc agatcaccct cgatttcgcc tactgggcca agaacggtcc ggccattgcg 2461 acacggtgga atgaatggct agtcaaactg caggtcgacg aacaaaaact catctcagaa 2521 gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg 2581 ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc 2641 cttatcatcc tcatcatgct ttggcagaag aagccacgtt agaagcttat cgataatcaa 2701 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 2761 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 2821 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 2881 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 2941 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 3001 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 3061 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 3121 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 3181 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 3241 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct 3301 cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc 3361 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 3421 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 3481 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 3541 ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt 3601 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 3661 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 3721 ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca 3781 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 3841 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 3901 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 3961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 4021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 4081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 4141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 4201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 4261 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 4321 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 4381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 4441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 4501 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 4561 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 4621 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 4681 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 4741 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 4801 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 4861 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 4921 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 4981 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 5041 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 5101 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 5161 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 5221 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 5281 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 5341 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 5401 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 5461 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 5521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 5581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 5641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 5701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 5761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 5821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 5881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 5941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 6001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 6061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 6121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 6181 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 6241 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 6301 gcggaaga //