LOCUS Exported 7101 bp ds-DNA circular SYN 23-MAY-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7101) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7101 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature complement(2565..2732) /label=ITR /note="ITR" misc_feature complement(3028..3668) /label=wpre /note="wpre" misc_feature 3553..3572 /label=nonstandard type: PCR_primer /note="WPRE rev WPRE rev ttaaagcagcgtatccacat" misc_feature complement(3670..3675) /label=Former PstI site /note="Former PstI site" misc_feature complement(3675..3680) /label=Former EcoRI site /note="Former EcoRI site" misc_feature 3715..3748 /label=loxp /note="loxp" misc_feature complement(3762..3767) /label=Former BglII site /note="Former BglII site" misc_feature 3819..3852 /label=loxp2272 /note="loxp2272" misc_feature 3883..3945 /label=IgG Leader /note="IgG Leader" misc_feature 3946..3951 /label=MCS /note="MCS" misc_feature 3952..4776 /label=Pf622 /note="Pf622" misc_feature 4249..4251 /label=F101L /note="F101L" misc_feature 4252..4254 /label=F102Y /note="F102Y" misc_feature 4357..4359 /label=Y137L /note="Y137L" misc_feature 4726..4728 /label=N260A /note="N260A" misc_feature 4777..5052 /label=SFGFP /note="SFGFP" misc_feature 5053..5070 /label=Linker /note="Linker" misc_feature 5071..5508 /label=SFGFP /note="SFGFP" misc_feature 5503..5505 /label=F145W /note="F145W" misc_feature 5509..5628 /label=Pf622 /note="Pf622" misc_feature 5674..5823 /label=PDGFR 513-561 misc_feature complement(5848..5881) /label=loxp /note="loxp" misc_feature complement(5883) /label=C to A switch indicated in sequencing /note="C to A switch indicated in sequencing" misc_feature complement(5944..5977) /label=loxp2272 /note="loxp2272" misc_feature complement(5985..6124) /label=Intron /note="Intron" misc_feature 5985..6004 /label=intron AscI 3'r /note="intron AscI 3'r" misc_feature complement(6125..6612) /label=synapsin promoter /note="synapsin promoter" misc_feature complement(6576..6594) /label=nonstandard type: PCR_primer /note="syn forward primer cactggacaagcacccaac" misc_feature complement(6592..6612) /label=Syn speI 5'f /note="Syn speI 5'f" misc_feature complement(6630..6648) /label=AA0208 SacI 480 f /note="AA0208 SacI 480 f" misc_feature complement(6677..6844) /label=ITR /note="ITR" ORIGIN 1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 1921 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 1981 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 2041 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 2101 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 2161 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 2221 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 2281 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 2341 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat 2401 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 2461 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 2521 cacgacgttg taaaacgacg gccagtgaat taggttaatt aaggctgcgc gctcgctcgc 2581 tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag 2641 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt 2701 taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag atccttatcg 2761 attttaccac atttgtagag gttttacttg ctttaaaaaa cctcccacat ctccccctga 2821 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 2881 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 2941 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgctcg aagcggccgc 3001 ccgggtcgac tctagatgca tgctcgagcg gccgcccggg tcgacggtat cgatgcgggg 3061 aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc 3121 gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt 3181 agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc caaggaaagg 3241 acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc 3301 agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga 3361 aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag 3421 caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag 3481 acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat 3541 gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa 3601 taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata agcttctcga 3661 gtggatcatt tgcagaattg gcccttttca gtgtttcgaa cctcttcgag ggacataact 3721 tcgtataatg tatgctatac gaagttatgg gtcgatggtg agatttggac tagagggtcg 3781 atggtgatgc ttggatcaaa ataggaagaa tcccaatgat aacttcgtat aaagtatcct 3841 atacgaagtt atactagtgg taccaccggt gatatcgcca ccatggagac agacacactc 3901 ctgctatggg tactgctgct ctgggttcca ggttccactg gtgacagatc tgaaagcatc 3961 aatttcgtga gctggggcgg tagcacccag gatgcgcaga agcaggcctg ggccgacccg 4021 ttcagcaagg ccagcggcat taccgtggtc caggatgggc ccaccgacta cggcaaactc 4081 aaggccatgg tcgaaagcgg caacgtgcag tgggacgtgg tggatgtaga ggccgacttc 4141 gccttgcgcg ccgccgccga aggcctgctc gaacccctgg atttctcggt gatccagcgc 4201 gacaagatcg acccgcgctt cgtctccgac catggcgtgg gctcgttcct gtattctttc 4261 gtgctcggct acaacgaagg caagctcgga gccagcaagc cccaggactg gaccgcgctg 4321 ttcgacacca agacctaccc cggcaaacgc gccctcctaa aatggccgag ccctggcgtg 4381 ctcgaactgg ccctgctcgc cgacggcgta ccggccgaca agctctaccc gctggacctg 4441 gaccgcgcct tcaagaaact cgacaccatc aagaaagaca tcgtctggtg gggcggcggt 4501 gcacagtcgc agcagctgct ggcctccggc gaagtcagca tgggccagtt ctggaacggt 4561 cgcatccacg ccctgcaaga agacggcgcg ccagtgggcg tgagctggaa gcagaacctg 4621 gtgatggccg acatcctcgt cgtgcctaaa ggcacgaaga acaaagccgc cgcgatgaag 4681 ttcctggcca gtgccagcag cgccaaaggc caggacgact tttccgccct gaccgcctat 4741 gctccagtga acatcgacag tgtgcagcgc ctcgaccttg cgcaggtccg gatcaccgcc 4801 gacaagcaga agaacggcat catggcgaac ttcaagatcc gccacaacgt ggaggacggc 4861 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 4921 ctgcccgaca accactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag 4981 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 5041 gagctgtaca agggcggtac cggagggagc atgagcaagg gcgaggagct gttcaccggg 5101 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgcgc 5161 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc 5221 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc 5281 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 5341 ggctacgtcc aggagcgcac catcagcttc aaggacgacg gcacctacaa gacccgcgcc 5401 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 5461 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actggaacgc caacctggca 5521 ccgaacctgc cgaccgccta cgtcaaggat cagatcaccc tcgatttcgc ctactgggcc 5581 aagaacggtc cggccattgc gacacggtgg aatgaatggc tagtcaaact gcaggtcgac 5641 gaacaaaaac tcatctcaga agaggatctg aatgctgtgg gccaggacac gcaggaggtc 5701 atcgtggtgc cacactcctt gccctttaag gtggtggtga tctcagccat cctggccctg 5761 gtggtgctca ccatcatctc ccttatcatc ctcatcatgc tttggcagaa gaagccacgt 5821 taggatatcg gatccgctag cactagtata acttcgtata gcatacatta tacgaagtta 5881 tgtgtcgatg gtgatgcttg gcaattcggg tcgatggtga agcattggtc ttcctatttt 5941 gatataactt cgtataggat actttatacg aagttatggg gatcatcacc tgtggagaga 6001 aaggcaaagt ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc 6061 tgtctcgaca agcccagttt ctattggtct ccttaaacct gtcttgtaac cttgatactt 6121 acctgatcca acggctgcga cttggggcag ggggtcctag gggtggtctg gccaggagcc 6181 gcgggggcgg actgcgcggt gcccaggagc acagctgcgc tctcaggcac gacacgactc 6241 ctccgctgcc caccgcagac tgaggcagcg ctgagtcgcc ggcgccgcag cgcagatggt 6301 cgcgcccgtg cccccctatc tcgcgcctcg cgtggtgcgg tccggctggg ccggcggcgg 6361 cgcggacgcg accaaggtgg ccgggaaggg gagtttgcgg gggaccggcg agtgacgtca 6421 gcgcgccttc agtgctgagg cggcggtggc gcgcgccgcc aggcgggggc gaaggcactg 6481 tccgcggtgc tgaagctggc agtgcgcacg cgcctcgccg catcctgttt cccctccccc 6541 tctctgatag gggatgcgca atttggggaa tgggggttgg gtgcttgtcc agtgggtcgg 6601 ggtcggtcgt caatttaaat actgaaaagg gcgaattcca gcacactgga gctcggatct 6661 tcctagagca tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac 6721 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc 6781 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc 6841 gcagcctaat taaggcctta attaaatctg gcgtaatcat ggtcatagct gtttcctgtg 6901 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 6961 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 7021 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 7081 ggcggtttgc gtattgggcg c //