LOCUS       Exported                7845 bp ds-DNA     circular SYN 23-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(2565..2732)
                     /label=ITR
                     /note="ITR"
     misc_feature    complement(3028..3668)
                     /label=wpre
                     /note="wpre"
     misc_feature    3553..3572
                     /label=nonstandard type: PCR_primer
                     /note="WPRE rev WPRE rev ttaaagcagcgtatccacat"
     misc_feature    complement(3670..3675)
                     /label=Former PstI site
                     /note="Former PstI site"
     misc_feature    complement(3675..3680)
                     /label=Former EcoRI site
                     /note="Former EcoRI site"
     misc_feature    3715..3748
                     /label=loxp
                     /note="loxp"
     misc_feature    complement(3762..3767)
                     /label=Former BglII site
                     /note="Former BglII site"
     misc_feature    3819..3852
                     /label=loxp2272
                     /note="loxp2272"
     misc_feature    3883..3945
                     /label=IgG Leader
                     /note="IgG Leader"
     misc_feature    3946..3951
                     /label=MCS
                     /note="MCS"
     misc_feature    3952..4776
                     /label=Pf622
                     /note="Pf622"
     misc_feature    4249..4251
                     /label=F101L
                     /note="F101L"
     misc_feature    4726..4728
                     /label=N260G
                     /note="N260G"
     misc_feature    4777..5052
                     /label=cpSFGFP 147-238
                     /note="cpSFGFP 147-238"
     misc_feature    4783..4785
                     /label=N149Q
                     /note="N149Q"
     misc_feature    4789..4791
                     /label=Y151R
                     /note="Y151R"
     misc_feature    4795..4797
                     /label=M153T
                     /note="M153T"
     misc_feature    4822..4824
                     /label=T162M
                     /note="T162M"
     misc_feature    5053..5070
                     /label=Linker
                     /note="Linker"
     misc_feature    5071..5508
                     /label=cpSFGFP 1-146
                     /note="cpSFGFP 1-146"
     misc_feature    5503..5505
                     /label=F145W
                     /note="F145W"
     misc_feature    5509..5628
                     /label=Pf622
                     /note="Pf622"
     misc_feature    5629..5673
                     /label=myc_epi
     misc_feature    5674..5820
                     /label=PDGFR 513-561
     misc_feature    5821..5862
                     /label=linker
                     /note="linker"
     misc_feature    5866..6579
                     /label=mRuby3
                     /note="mRuby3"
     misc_feature    5974..5976
                     /label=N33R
                     /note="N33R"
     misc_feature    5983..5985
                     /label=M36E
                     /note="M36E"
     misc_feature    5989..5991
                     /label=T38V
                     /note="T38V"
     misc_feature    6097..6099
                     /label=K74A
                     /note="K74A"
     misc_feature    6100..6102
                     /label=G75D
                     /note="G75D"
     misc_feature    6190..6192
                     /label=M105T
                     /note="M105T"
     misc_feature    6217..6219
                     /label=C114E
                     /note="C114E"
     misc_feature    6229..6231
                     /label=H118N
                     /note="H118N"
     misc_feature    6235..6237
                     /label=Q120K
                     /note="Q120K"
     misc_feature    6352..6354
                     /label=H159D
                     /note="H159D"
     misc_feature    6355..6357
                     /label=M160I
                     /note="M160I"
     misc_feature    6388..6390
                     /label=S171H
                     /note="S171H"
     misc_feature    6394..6396
                     /label=S173N
                     /note="S173N"
     misc_feature    6451..6453
                     /label=I192V
                     /note="I192V"
     misc_feature    6481..6483
                     /label=L202I
                     /note="L202I"
     misc_feature    6502..6504
                     /label=M209T
                     /note="M209T"
     misc_feature    6505..6507
                     /label=F210Y
                     /note="F210Y"
     misc_feature    6523..6525
                     /label=H216V
                     /note="H216V"
     misc_feature    6538..6540
                     /label=F221Y
                     /note="F221Y"
     misc_feature    6541..6543
                     /label=A222S
                     /note="A222S"
     misc_feature    6544..6546
                     /label=G223N
                     /note="G223N"
     misc_feature    complement(6592..6625)
                     /label=loxp
                     /note="loxp"
     misc_feature    complement(6627)
                     /label=C to A switch indicated in sequencing
                     /note="C to A switch indicated in sequencing"
     misc_feature    complement(6688..6721)
                     /label=loxp2272
                     /note="loxp2272"
     misc_feature    complement(6729..6868)
                     /label=Intron
                     /note="Intron"
     misc_feature    6729..6748
                     /label=intron AscI 3'r
                     /note="intron AscI 3'r"
     misc_feature    complement(6869..7356)
                     /label=synapsin promoter
                     /note="synapsin promoter"
     misc_feature    complement(7320..7338)
                     /label=nonstandard type: PCR_primer
                     /note="syn forward primer cactggacaagcacccaac"
     misc_feature    complement(7336..7356)
                     /label=Syn speI 5'f
                     /note="Syn speI 5'f"
     misc_feature    complement(7374..7392)
                     /label=AA0208 SacI 480 f
                     /note="AA0208 SacI 480 f"
     misc_feature    complement(7421..7588)
                     /label=ITR
                     /note="ITR"
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     1981 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2041 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2101 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2161 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2221 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     2281 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2341 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     2401 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     2461 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     2521 cacgacgttg taaaacgacg gccagtgaat taggttaatt aaggctgcgc gctcgctcgc
     2581 tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag
     2641 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt
     2701 taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag atccttatcg
     2761 attttaccac atttgtagag gttttacttg ctttaaaaaa cctcccacat ctccccctga
     2821 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2881 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2941 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgctcg aagcggccgc
     3001 ccgggtcgac tctagatgca tgctcgagcg gccgcccggg tcgacggtat cgatgcgggg
     3061 aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc
     3121 gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt
     3181 agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc caaggaaagg
     3241 acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc
     3301 agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga
     3361 aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag
     3421 caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag
     3481 acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat
     3541 gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa
     3601 taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata agcttctcga
     3661 gtggatcatt tgcagaattg gcccttttca gtgtttcgaa cctcttcgag ggacataact
     3721 tcgtataatg tatgctatac gaagttatgg gtcgatggtg agatttggac tagagggtcg
     3781 atggtgatgc ttggatcaaa ataggaagaa tcccaatgat aacttcgtat aaagtatcct
     3841 atacgaagtt atactagtgg taccaccggt gatatcgcca ccatggagac agacacactc
     3901 ctgctatggg tactgctgct ctgggttcca ggttccactg gtgacagatc tgaaagcatc
     3961 aatttcgtga gctggggcgg tagcacccag gatgcgcaga agcaggcctg ggccgacccg
     4021 ttcagcaagg ccagcggcat taccgtggtc caggatgggc ccaccgacta cggcaaactc
     4081 aaggccatgg tcgaaagcgg caacgtgcag tgggacgtgg tggatgtaga ggccgacttc
     4141 gccttgcgcg ccgccgccga aggcctgctc gaacccctgg atttctcggt gatccagcgc
     4201 gacaagatcg acccgcgctt cgtctccgac catggcgtgg gctcgttcct gttctctttc
     4261 gtgctcggct acaacgaagg caagctcgga gccagcaagc cccaggactg gaccgcgctg
     4321 ttcgacacca agacctaccc cggcaaacgc gccctctaca aatggccgag ccctggcgtg
     4381 ctcgaactgg ccctgctcgc cgacggcgta ccggccgaca agctctaccc gctggacctg
     4441 gaccgcgcct tcaagaaact cgacaccatc aagaaagaca tcgtctggtg gggcggcggt
     4501 gcacagtcgc agcagctgct ggcctccggc gaagtcagca tgggccagtt ctggaacggt
     4561 cgcatccacg ccctgcaaga agacggcgcg ccagtgggcg tgagctggaa gcagaacctg
     4621 gtgatggccg acatcctcgt cgtgcctaaa ggcacgaaga acaaagccgc cgcgatgaag
     4681 ttcctggcca gtgccagcag cgccaaaggc caggacgact tttccgccct gaccgcctat
     4741 gctccagtga acatcgacag tgtgcagcgc ctcgaccttg cgcaggtccg gatcaccgcc
     4801 gacaagcaga agaacggcat catggcgaac ttcaagatcc gccacaacgt ggaggacggc
     4861 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4921 ctgcccgaca accactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag
     4981 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5041 gagctgtaca agggcggtac cggagggagc atgagcaagg gcgaggagct gttcaccggg
     5101 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgcgc
     5161 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
     5221 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5281 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5341 ggctacgtcc aggagcgcac catcagcttc aaggacgacg gcacctacaa gacccgcgcc
     5401 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5461 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actggaacgc caacctggca
     5521 ccgaacctgc cgaccgccta cgtcaaggat cagatcaccc tcgatttcgc ctactgggcc
     5581 aagaacggtc cggccattgc gacacggtgg aatgaatggc tagtcaaact gcaggtcgac
     5641 gaacaaaaac tcatctcaga agaggatctg aatgctgtgg gccaggacac gcaggaggtc
     5701 atcgtggtgc cacactcctt gccctttaag gtggtggtga tctcagccat cctggccctg
     5761 gtggtgctca ccatcatctc ccttatcatc ctcatcatgc tttggcagaa gaagccacgt
     5821 tctagaggct ccggaaatgc tgtgggccag gacacgcagg agcgtatggt gtctaagggc
     5881 gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc
     5941 caccaattca aatgcacagg tgaaggagaa ggcaggccgt acgagggagt gcaaaccatg
     6001 aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg
     6061 ttcatgtatg gcagccgtac ttttatcaag tacccggcag acattcctga tttctttaaa
     6121 cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga tggtggagtc
     6181 gtcaccgtca cgcaggacac cagccttgag gatggcgaac tcgtttacaa cgtcaaagtc
     6241 agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa gggttgggag
     6301 cctaatacag agatgatgta tccagcagat ggtggtctga ggggatacac tgatattgca
     6361 ctgaaagttg atggtggtgg ccatctgcat tgcaatttcg taacaactta caggtcaaaa
     6421 aagaccgtcg ggaacatcaa gatgcccggt gtccatgccg ttgatcaccg cctggaaagg
     6481 atagaggaaa gtgacaatga aacgtacgta gtacaacgcg aagtcgcagt tgccaagtac
     6541 tccaatcttg gtggtggtat ggatgaactt tataagtaag ctagcactag tataacttcg
     6601 tatagcatac attatacgaa gttatgtgtc gatggtgatg cttggcaatt cgggtcgatg
     6661 gtgaagcatt ggtcttccta ttttgatata acttcgtata ggatacttta tacgaagtta
     6721 tggggatcat cacctgtgga gagaaaggca aagtggatgt cagtaagacc aataggtgcc
     6781 tatcagaaac gcaagagtct tctctgtctc gacaagccca gtttctattg gtctccttaa
     6841 acctgtcttg taaccttgat acttacctga tccaacggct gcgacttggg gcagggggtc
     6901 ctaggggtgg tctggccagg agccgcgggg gcggactgcg cggtgcccag gagcacagct
     6961 gcgctctcag gcacgacacg actcctccgc tgcccaccgc agactgaggc agcgctgagt
     7021 cgccggcgcc gcagcgcaga tggtcgcgcc cgtgcccccc tatctcgcgc ctcgcgtggt
     7081 gcggtccggc tgggccggcg gcggcgcgga cgcgaccaag gtggccggga aggggagttt
     7141 gcgggggacc ggcgagtgac gtcagcgcgc cttcagtgct gaggcggcgg tggcgcgcgc
     7201 cgccaggcgg gggcgaaggc actgtccgcg gtgctgaagc tggcagtgcg cacgcgcctc
     7261 gccgcatcct gtttcccctc cccctctctg ataggggatg cgcaatttgg ggaatggggg
     7321 ttgggtgctt gtccagtggg tcggggtcgg tcgtcaattt aaatactgaa aagggcgaat
     7381 tccagcacac tggagctcgg atcttcctag agcatggcta cgtagataag tagcatggcg
     7441 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     7501 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     7561 cggcctcagt gagcgagcga gcgcgcagcc taattaaggc cttaattaaa tctggcgtaa
     7621 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7681 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7741 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgc
//
