LOCUS Exported 6811 bp ds-DNA circular SYN 23-MAY-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6811) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6811 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 637..656 /label=T7 misc_feature 736..843 /label=Cox8 sequence (mitochondria matrix targeting) /note="Cox8 sequence (mitochondria matrix targeting)" misc_feature 850..1674 /label=Pf622 /note="Pf622" misc_feature 1147..1149 /label=F101L /note="F101L" misc_feature 1624..1626 /label=N260G /note="N260G" misc_feature 1675..1950 /label=cpSFGFP /note="cpSFGFP" misc_feature 1681..1683 /label=N149Q /note="N149Q" misc_feature 1687..1689 /label=Y151R /note="Y151R" misc_feature 1693..1695 /label=M153T /note="M153T" misc_feature 1720..1722 /label=K162M /note="K162M" misc_feature 1951..1968 /label=Linker /note="Linker" misc_feature 1969..2406 /label=cpSFGFP /note="cpSFGFP" misc_feature 2401..2403 /label=F145W /note="F145W" misc_feature 2407..2541 /label=Pf622 /note="Pf622" misc_feature 2560..2577 /label=BGH_rev rep_origin complement(2904..3586) /direction=LEFT /label=ColE1 origin CDS complement(5364..6023) /label=AmpR rep_origin complement(6353..6809) /direction=LEFT /label=F1 ori ORIGIN 1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 181 aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc 241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct 601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac 661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc 721 ttggggatat ccaccatgag cgtgctgacc cccctgctgc tgaggggcct gaccggcagc 781 gccaggaggc tgcccgtgcc cagggccaag atccacagcc tgggcgaccc ccccgtggcc 841 accagatctg aaagcatcaa tttcgtgagc tggggcggta gcacccagga tgcgcagaag 901 caggcctggg ccgacccgtt cagcaaggcc agcggcatta ccgtggtcca ggatgggccc 961 accgactacg gcaaactcaa ggccatggtc gaaagcggca acgtgcagtg ggacgtggtg 1021 gatgtagagg ccgacttcgc cttgcgcgcc gccgccgaag gcctgctcga acccctggat 1081 ttctcggtga tccagcgcga caagatcgac ccgcgcttcg tctccgacca tggcgtgggc 1141 tcgttcctgt tctctttcgt gctcggctac aacgaaggca agctcggagc cagcaagccc 1201 caggactgga ccgcgctgtt cgacaccaag acctaccccg gcaaacgcgc cctctacaaa 1261 tggccgagcc ctggcgtgct cgaactggcc ctgctcgccg acggcgtacc ggccgacaag 1321 ctctacccgc tggacctgga ccgcgccttc aagaaactcg acaccatcaa gaaagacatc 1381 gtctggtggg gcggcggtgc acagtcgcag cagctgctgg cctccggcga agtcagcatg 1441 ggccagttct ggaacggtcg catccacgcc ctgcaagaag acggcgcgcc agtgggcgtg 1501 agctggaagc agaacctggt gatggccgac atcctcgtcg tgcctaaagg cacgaagaac 1561 aaagccgccg cgatgaagtt cctggccagt gccagcagcg ccaaaggcca ggacgacttt 1621 tccgccctga ccgcctatgc tccagtgaac atcgacagtg tgcagcgcct cgaccttgcg 1681 caggtccgga tcaccgccga caagcagaag aacggcatca tggcgaactt caagatccgc 1741 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc 1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1921 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat gagcaagggc 1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 2041 cacaagttca gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg 2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc 2281 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 2401 tggaacgcca acctggcacc gaacctgccg accgcctacg tcaaggatca gatcaccctc 2461 gatttcgcct actgggccaa gaacggtccg gccattgcga cacggtggaa tgaatggcta 2521 gtcaaactgc aggtcgacta ggcggccgct cgagatcagc ctcgactgtg ccttctagtt 2581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 2641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 2701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 2761 ggcgtgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agtggcggta 2821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 2881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 2941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 3001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 3061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 3121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 3181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 3241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 3301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 3361 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 3421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 3481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 3541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 3601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 3661 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct 3721 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa 3781 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga 3841 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt 3901 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac 3961 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg 4021 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc 4081 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc 4141 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt 4201 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat 4261 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca 4321 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggct 4381 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttgatcat 4441 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt 4501 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca 4561 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt 4621 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag 4681 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg 4741 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca 4801 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca 4861 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc 4921 atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc tcctcactac 4981 ttctggaata gctcagaggc cgaggaggcg gcctcggcct ctgcataaat aaaaaaaatt 5041 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga 5101 gttaggggcg ggactatggt tgctgactaa ttgagatgct tgctttgcat acttctgcct 5161 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg cttgctttgc 5221 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac 5281 agctggttct ttccgcctca ggactcttcc tttttcaata aatcaatcta aagtatatat 5341 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 5401 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 5461 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 5521 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 5581 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 5641 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 5701 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 5761 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 5821 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 5881 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 5941 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 6001 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 6061 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 6121 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 6181 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 6241 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 6301 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 6361 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 6421 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 6481 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 6541 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 6601 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 6661 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 6721 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 6781 tttaacaaaa tattaacgct tacaatttac g //