LOCUS Exported 7555 bp ds-DNA circular SYN 23-MAY-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7555) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7555 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 637..656 /label=T7 misc_feature 736..843 /label=Cox8 sequence (mitochondria matrix targeting) /note="Cox8 sequence (mitochondria matrix targeting)" misc_feature 850..1674 /label=Pf622 /note="Pf622" misc_feature 1147..1149 /label=F101L /note="F101L" misc_feature 1150..1152 /label=F102G /note="F102G" misc_feature 1624..1626 /label=N260G /note="N260G" misc_feature 1675..1950 /label=cpSFGFP /note="cpSFGFP" misc_feature 1681..1683 /label=N149Q /note="N149Q" misc_feature 1687..1689 /label=Y151R /note="Y151R" misc_feature 1693..1695 /label=M153T /note="M153T" misc_feature 1720..1722 /label=K162M /note="K162M" misc_feature 1951..1968 /label=Linker /note="Linker" misc_feature 1969..2406 /label=cpSFGFP /note="cpSFGFP" misc_feature 2401..2403 /label=F145W /note="F145W" misc_feature 2407..2532 /label=Pf622 /note="Pf622" misc_feature 2533..2571 /label=Linker /note="Linker" misc_feature 2572..3285 /label=mRuby3 /note="mRuby3" misc_feature 3304..3321 /label=BGH_rev rep_origin complement(3648..4330) /direction=LEFT /label=ColE1 origin CDS complement(6108..6767) /label=AmpR rep_origin complement(7097..7553) /direction=LEFT /label=F1 ori ORIGIN 1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 181 aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc 241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct 601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac 661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc 721 ttggggatat ccaccatgag cgtgctgacc cccctgctgc tgaggggcct gaccggcagc 781 gccaggaggc tgcccgtgcc cagggccaag atccacagcc tgggcgaccc ccccgtggcc 841 accagatctg aaagcatcaa tttcgtgagc tggggcggta gcacccagga tgcgcagaag 901 caggcctggg ccgacccgtt cagcaaggcc agcggcatta ccgtggtcca ggatgggccc 961 accgactacg gcaaactcaa ggccatggtc gaaagcggca acgtgcagtg ggacgtggtg 1021 gatgtagagg ccgacttcgc cttgcgcgcc gccgccgaag gcctgctcga acccctggat 1081 ttctcggtga tccagcgcga caagatcgac ccgcgcttcg tctccgacca tggcgtgggc 1141 tcgttcctgg gctctttcgt gctcggctac aacgaaggca agctcggagc cagcaagccc 1201 caggactgga ccgcgctgtt cgacaccaag acctaccccg gcaaacgcgc cctctacaaa 1261 tggccgagcc ctggcgtgct cgaactggcc ctgctcgccg acggcgtacc ggccgacaag 1321 ctctacccgc tggacctgga ccgcgccttc aagaaactcg acaccatcaa gaaagacatc 1381 gtctggtggg gcggcggtgc acagtcgcag cagctgctgg cctccggcga agtcagcatg 1441 ggccagttct ggaacggtcg catccacgcc ctgcaagaag acggcgcgcc agtgggcgtg 1501 agctggaagc agaacctggt gatggccgac atcctcgtcg tgcctaaagg cacgaagaac 1561 aaagccgccg cgatgaagtt cctggccagt gccagcagcg ccaaaggcca ggacgacttt 1621 tccgccctga ccgcctatgc tccagtgaac atcgacagtg tgcagcgcct cgaccttgcg 1681 caggtccgga tcaccgccga caagcagaag aacggcatca tggcgaactt caagatccgc 1741 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc 1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1921 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat gagcaagggc 1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 2041 cacaagttca gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg 2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc 2281 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 2401 tggaacgcca acctggcacc gaacctgccg accgcctacg tcaaggatca gatcaccctc 2461 gatttcgcct actgggccaa gaacggtccg gccattgcga cacggtggaa tgaatggcta 2521 gtcaaactgc agggctccgg aaatgctgtg ggccaggaca cgcaggagcg tatggtgtct 2581 aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc 2641 aacggccacc aattcaaatg cacaggtgaa ggagaaggca ggccgtacga gggagtgcaa 2701 accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga cattcttgcc 2761 acgtcgttca tgtatggcag ccgtactttt atcaagtacc cggcagacat tcctgatttc 2821 tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata cgaagatggt 2881 ggagtcgtca ccgtcacgca ggacaccagc cttgaggatg gcgaactcgt ttacaacgtc 2941 aaagtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa gaccaagggt 3001 tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagggg atacactgat 3061 attgcactga aagttgatgg tggtggccat ctgcattgca atttcgtaac aacttacagg 3121 tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc atgccgttga tcaccgcctg 3181 gaaaggatag aggaaagtga caatgaaacg tacgtagtac aacgcgaagt cgcagttgcc 3241 aagtactcca atcttggtgg tggtatggat gaactttata agtaggcggc cgctcgagat 3301 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 3361 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 3421 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 3481 gggaggattg ggaagacaat agcaggcgtg ctggggatgc ggtgggctct atggcttctg 3541 aggcggaaag aaccagtggc ggtaatacgg ttatccacag aatcagggga taacgcagga 3601 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 3661 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 3721 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 3781 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 3841 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 3901 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 3961 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 4021 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 4081 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 4141 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 4201 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 4261 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 4321 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 4381 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg 4441 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa 4501 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc 4561 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt 4621 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc 4681 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag 4741 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg 4801 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg 4861 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga 4921 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac 4981 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga 5041 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca 5101 cgacgagatc ctcgccgtcg ggcttgctcg ccttgagcct ggcgaacagt tcggctggcg 5161 cgagcccctg atgctcttga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg 5221 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat 5281 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg 5341 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga 5401 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg 5461 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc 5521 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc 5581 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt 5641 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca aaagcctagg 5701 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgagga ggcggcctcg 5761 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc 5821 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga 5881 tgcttgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct 5941 gactaattga gatgcttgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca 6001 caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc ttcctttttc 6061 aataaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 6121 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 6181 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 6241 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 6301 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 6361 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 6421 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 6481 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 6541 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 6601 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 6661 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 6721 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 6781 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 6841 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 6901 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 6961 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7021 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7081 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 7141 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 7201 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 7261 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 7321 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 7381 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 7441 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 7501 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacg //