LOCUS Exported 10818 bp ds-DNA circular SYN 23-MAY-2018 DEFINITION Ligation of SPy_1046-with-NLS-flag into pST1374. ACCESSION GS201363-1 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10818) AUTHORS Self JOURNAL Unpublished. REFERENCE 2 (bases 1 to 10818) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com COMMENT SECID/File created by SciEd Central, Scientific & Educational Software SECNOTES|Vector molecule: pST174 Fragment ends: ApaI and XbaI Fragment size: 5108 Insert molecule: SPy_1046-with-NLS-flag Fragment ends: XbaI and ApaI Fragment size: 4114 FEATURES Location/Qualifiers source 1..10818 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 910..930 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature 910..930 /label=SV40 NLS misc_feature 949..1557 /label=DNMT3LCD CDS 1585..1602 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" exon 1606..2463 /label=DNMT3A CD misc_feature 2464..2510 /label=Linker2 CDS 2512..6663 /codon_start=1 /label=dCas9 /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVSSGGAAG" misc_feature 2536..2538 /label=D10A /note="mutation" misc_feature 5026..5027 /label=H840A /note="mutation" misc_feature 5095..5097 /label=N863A /note="mutation" misc_feature 6613..6660 /label=NLS CDS 6619..6639 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6682..6723 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 6733..6750 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 6779..7003 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7049..7477 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 7491..7821 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7672..7807 /label=SV40 ori /note="SV40 origin of replication" promoter 7869..7916 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7935..8333 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 8491..8612 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8661..8677) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8685..8701 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8709..8739) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8754..8775 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(9063..9651) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9822..10682) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10683..10787) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 accatgggac ctaagaaaaa gaggaaggtg gcggccgctg gcggcagcat gttcgaaacc 961 gtgcctgtgt ggaggagaca gccagtccgg gtgctgtccc tttttgaaga catcaagaaa 1021 gagctgacga gtttgggctt tttggaaagt ggttctgacc cgggacaact gaagcatgtg 1081 gttgatgtca cagacacagt gaggaaggat gtggaggagt ggggaccctt cgatcttgtg 1141 tacggcgcca cacctcccct gggccacacc tgtgaccgtc ctcccagctg gtacctgttc 1201 cagttccacc ggctcctgca gtacgcacgg cccaagccag gcagccccag gcccttcttc 1261 tggatgttcg tggacaatct ggtgctgaac aaggaagacc tggacgtcgc atctcgcttc 1321 ctggagatgg agccagtcac catcccagat gtccacggcg gatccttgca gaatgctgtc 1381 cgcgtgtgga gcaacatccc agccataagg agcagcaggc actgggctct ggtttcggaa 1441 gaagaattgt ccctgctggc ccagaacaag cagagctcga agctcgcggc caagtggccc 1501 accaagctgg tgaagaactg ctttctcccc ctaagagaat atttcaagta tttttcaagc 1561 agcgggcgca gcttcagcag cggactagtg ccccgcggca gccacccagc tgagaagagg 1621 aagcccatcc gggtgctgtc tctctttgat ggaatcgcta cagggctcct ggtgctgaag 1681 gacttgggca ttcaggtgga ccgctacatt gcctcggagg tgtgtgagga ctccatcacg 1741 gtgggcatgg tgcggcacca ggggaagatc atgtacgtcg gggacgtccg cagcgtcaca 1801 cagaagcata tccaggagtg gggcccattc gatctggtga ttgggggcag tccctccaat 1861 gacctctcca tcgtcaaccc tgctcgcaag ggcctctacg agggcactgg ccggctcttc 1921 tttgagttct accgcctcct gcatgatgcg cggcccaagg agggagatga tcgccccttc 1981 ttctggctct ttgagaatgt ggtggccatg ggcgttagtg acaagaggga catctcgcga 2041 tttctcgagt ccaaccctgt gatgattgat gccaaagaag tgtcagctgc acacagggcc 2101 cgctacttct ggggtaacct tcccggtatg aacaggccgt tggcatccac tgtgaatgat 2161 aagctggagc tgcaggagtg tctggagcat ggcaggatag ccaagttcag caaagtgagg 2221 accattacta cgaggtcaaa ctccataaag cagggcaaag accagcattt tcctgtcttc 2281 atgaatgaga aagaggacat cttatggtgc actgaaatgg aaagggtatt tggtttccca 2341 gtccactata ctgacgtctc caacatgagc cgcttggcga ggcagagact gctgggccgg 2401 tcatggagcg tgccagtcat ccgccacctc ttcgctccgc tgaaggagta ttttgcgtgt 2461 gtgagcggca gcgagacccc cggtaccagc gagagcgcca cccccgagag cgacaagaaa 2521 tactctattg gactggctat cgggacaaac tccgttggct gggccgtcat aaccgacgag 2581 tataaggtgc caagcaagaa attcaaggtg ctgggtaata ctgaccgcca ttcaatcaag 2641 aagaacctga tcggagcact cctcttcgac tccggtgaaa ccgctgaagc tactcggctg 2701 aagcggaccg caaggcggag atacacccgc cgcaagaatc ggatatgtta tctgcaagag 2761 atctttagca acgaaatggc taaggtggac gactccttct ttcaccgcct ggaagagagc 2821 tttctggtgg aggaggataa gaaacacgag aggcacccta tattcggaaa tatcgtggat 2881 gaggtggctt accatgaaaa gtatcctaca atctaccatc tgaggaagaa gctggtggac 2941 agcaccgata aagcagacct gaggctcatc tatctggccc tggctcatat gataaagttt 3001 agaggacact ttctgatcga gggcgacctg aatcccgata attccgatgt ggataaactc 3061 ttcattcaac tggtgcagac atataaccaa ctgttcgagg agaatcccat aaacgcttct 3121 ggtgtggatg ccaaggctat tctgtccgct cggctgtcca agtcacgcag actggagaat 3181 ctgattgccc aactgccagg agaaaagaag aacggcctgt ttgggaacct catcgccctg 3241 agcctgggcc tgacacctaa cttcaagtcc aattttgatc tggccgaaga tgctaaactc 3301 cagctctcca aggacaccta tgacgatgat ctggacaacc tgctcgcaca gataggcgac 3361 cagtacgccg atctctttct ggctgctaag aatctctccg acgccattct gctgagcgac 3421 atactccggg tcaacactga gatcaccaaa gcacctctga gcgcctccat gataaaacgc 3481 tatgatgaac accatcaaga cctgactctg ctcaaagccc tcgtgaggca acagctgcca 3541 gagaagtaca aagagatatt cttcgaccag agcaagaatg gatatgccgg atacatcgat 3601 ggcggagcat cacaggaaga attttacaag ttcatcaaac caatcctcga gaagatggac 3661 ggtactgaag agctgctggt gaagctgaac agggaggacc tgctgaggaa gcagaggacc 3721 tttgataatg gctccattcc acatcagata cacctgggag agctgcatgc aatcctccgc 3781 aggcaggagg atttctatcc tttcctgaag gataaccggg agaagataga gaagatcctg 3841 accttcagga tcccttatta cgtcggccct ctggctagag gcaactcccg cttcgcttgg 3901 atgaccagga aatctgagga gacaattact ccttggaact tcgaagaggt cgtggataag 3961 ggcgcaagcg cccagtcatt catcgaacgg atgaccaatt tcgataagaa cctgccaaac 4021 gagaaggtcc tgcccaaaca ttcactcctg tacgagtatt tcaccgtcta taacgagctg 4081 actaaagtga agtacgtgac cgagggcatg aggaagcctg ccttcctgtc cggagagcag 4141 aagaaggcta tcgttgatct gctcttcaag actaatagaa aggtgacagt gaagcagctc 4201 aaggaggatt actttaagaa gatcgaatgc tttgactcag tggaaatctc tggcgtggag 4261 gaccgcttta atgccagcct gggcacttac catgatctgc tgaagataat caaagacaaa 4321 gatttcctcg ataatgagga gaacgaggac atcctggaag atatcgtgct gaccctgact 4381 ctgttcgagg atagagagat gatcgaagag cgcctgaaga cctatgccca tctgtttgac 4441 gataaagtca tgaaacagct caagcggcgg cgctacactg ggtggggtag actctccagg 4501 aaactcataa acggcatccg cgacaaacag agcggaaaga ccatcctgga tttcctgaaa 4561 tccgacggat tcgctaacag gaacttcatg caactgattc acgatgactc tctgacattt 4621 aaagaggaca tccagaaggc acaggtgagc ggtcaaggcg acagcctgca cgagcacatc 4681 gccaacctcg ctggatcacc cgccataaag aagggaatac tgcagacagt caaggtcgtg 4741 gacgaactcg tcaaagtgat gggtcggcac aagccagaga atatcgttat cgaaatggca 4801 agggagaacc aaaccaccca gaagggccag aagaactctc gggaacggat gaaaagaatc 4861 gaagagggaa ttaaggagct gggatctcag atactgaagg agcaccctgt ggagaataca 4921 cagctccaga acgagaaact ctacctgtac tacctccaga acgggcggga catgtacgtt 4981 gaccaggaac tcgacatcaa ccggctgtcc gattatgacg tggacgctat tgttccacag 5041 tccttcctca aagatgactc cattgacaac aaggtgctga ccagatccga taaggcccgc 5101 ggtaagtctg acaatgttcc atcagaagag gtggtcaaga agatgaagaa ttactggcgg 5161 cagctcctca acgccaaact gatcacccag cggaagtttg acaatctgac taaggcagaa 5221 agaggaggtc tgagcgaact cgacaaggcc ggctttatta agaggcaact ggtcgaaaca 5281 cgccagatta ccaaacacgt ggcacaaatc ctcgactcta ggatgaacac taagtacgat 5341 gagaacgata agctgatcag ggaagtgaaa gtgataactc tgaagagcaa gctggtgtct 5401 gacttccgga aggactttca attctacaaa gttcgcgaaa taaacaatta ccatcatgct 5461 cacgatgcct atctcaatgc tgtcgttggc accgccctga tcaagaaata ccctaaactg 5521 gagtctgagt tcgtgtacgg tgactataaa gtctacgatg tgaggaagat gatagcaaag 5581 tctgagcaag agattggcaa agccaccgcc aagtacttct tctactctaa tatcatgaat 5641 ttctttaaga ctgagataac cctggctaac ggcgaaatcc ggaagcgccc actgatcgaa 5701 acaaacggag aaacaggaga aatcgtgtgg gataaaggca gggacttcgc aactgtgcgg 5761 aaggtgctgt ccatgccaca agtcaatatc gtgaagaaga ccgaagtgca gaccggcgga 5821 ttctcaaagg agagcatcct gccaaagcgg aactctgaca agctgatcgc caggaagaaa 5881 gattgggacc caaagaagta tggcggtttc gattccccta cagtggctta ttccgttctg 5941 gtcgtggcaa aagtggagaa aggcaagtcc aagaaactca agtctgttaa ggagctgctc 6001 ggaattacta ttatggagag atccagcttc gagaagaatc caatcgattt cctggaagct 6061 aagggctata aagaagtgaa gaaagatctc atcatcaaac tgcccaagta ctctctcttt 6121 gagctggaga atggtaggaa gcggatgctg gcctccgccg gagagctgca gaaaggaaac 6181 gagctggctc tgccctccaa atacgtgaac ttcctgtatc tggcctccca ctacgagaaa 6241 ctcaaaggta gccctgaaga caatgagcag aagcaactct ttgttgagca acataaacac 6301 tacctggacg aaatcattga acagattagc gagttcagca agcgggttat tctggccgat 6361 gcaaacctcg ataaagtgct gagcgcatat aataagcaca gggacaagcc aattcgcgaa 6421 caagcagaga atattatcca cctctttact ctgactaatc tgggcgctcc tgctgccttc 6481 aagtatttcg atacaactat tgacaggaag cggtacacct ctaccaaaga agttctcgat 6541 gccaccctga tacaccagtc aattaccgga ctgtacgaga ctcgcatcga cctgtctcag 6601 ctcggcggcg acggttctcc caagaagaag aggaaagtct cgagcggtgg agctgcagga 6661 taggaattcg ggcccttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct 6721 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact 6781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 6841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 6901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 6961 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 7021 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 7081 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 7141 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 7201 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 7261 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 7321 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 7381 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta 7441 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 7501 tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc 7561 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 7621 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 7681 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 7741 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 7801 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat 7861 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg 7921 tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag 7981 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc 8041 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt 8101 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta 8161 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgtcgacagg 8221 tgcttctcga tctgcatcct gggatcaaag cgatagtgaa ggacagtgat ggacagccga 8281 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacttc 8341 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat 8401 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 8461 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 8521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 8581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 8641 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 8701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 8761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 8821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 8881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 8941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 9001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 9061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 9241 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 9481 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 9541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 9601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 9661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 9721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 9781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 9841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 9901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 9961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 10021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 10081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 10141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 10201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 10261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 10321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 10381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 10441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 10501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 10561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 10621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 10681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 10741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 10801 aaagtgccac ctgacgtc //