LOCUS       pMTB                    6346 bp    DNA     circular SYN 20-NOV-2007
DEFINITION  Transposon vector T2AL200R150G DNA, complete sequence.
ACCESSION   AB262452
VERSION     AB262452.1  GI:110082641
SOURCE      Transposon vector T2AL200R150G.
  ORGANISM  Transposon vector T2AL200R150G
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Urasaki,A., Morvan,G. and Kawakami,K.
  TITLE     Functional dissection of the Tol2 transposable element identified
            the minimal cis-sequence and a highly repetitive sequence in the
            subterminal region essential for transposition
  JOURNAL   Genetics 174 (2), 639-649 (2006)
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Urasaki,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2006) Akihiro Urasaki, National Institute of
            Genetics, Division of Molecular and Developmental Biology; 1111
            Yata, Mishima, Shizuoka 411-8540, Japan
            (E-mail:aurasaki@lab.nig.ac.jp, Tel:81-55-981-6739,
            Fax:81-55-981-5827)
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|463531442|
COMMENT     VNTDBDATE|463531442|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMTB|
COMMENT     VNTAUTHORNAME|Sean Megason|
COMMENT     VNTAUTHOREML|megason@caltech.edu|
COMMENT     VNTAUTHORWWW|www.digitalfish.org|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     repeat_region   1469..1668
                     /dnas_title="Tol2-L200"
                     /mobile_element="transposon:T2AL200R150G"
                     /vntifkey="34"
                     /label=Tol2-L200
                     /note="left end of Tol2"
     repeat_region   5587..5736
                     /dnas_title="Tol2-R150"
                     /mobile_element="transposon:T2AL200R150G"
                     /vntifkey="34"
                     /label=Tol2-R150
                     /note="right end of Tol2"
     polyA_signal    5361..5556
                     /dnas_title="SV40pA"
                     /vntifkey="25"
                     /label=SV40pA
     promoter        4383..4405
                     /dnas_title="SP6 promoter: CATACGATTTAGGTGACACTATAG "
                     /vntifkey="30"
                     /label=SP6 promoter: CATACGATTTAGGTGACACTATAG
     intron          3004..4364
                     /dnas_title="first intron Bactin2"
                     /vntifkey="15"
                     /label=first intron Bactin2
     exon            2918..3003
                     /dnas_title="first exon Bactin2 (non-coding)"
                     /vntifkey="61"
                     /label=first exon Bactin2 (non-coding)
     exon            4365..4373
                     /dnas_title="second exon Bactin2"
                     /vntifkey="61"
                     /label=second exon Bactin2
     primer          complement(4354..4383)
                     /dnas_title="bactin2-R"
                     /vntifkey="27"
                     /label=bactin2-R
     promoter        1682..2917
                     /dnas_title="Bactin2 promoter"
                     /vntifkey="29"
                     /label=Bactin2 promoter
     misc_feature    5320..5360
                     /dnas_title="MCS-1"
                     /vntifkey="21"
                     /label=MCS-1
     misc_feature    5557..5580
                     /dnas_title="MCS-2"
                     /vntifkey="21"
                     /label=MCS-2
     promoter        4409..4430
                     /dnas_title="T7 "
                     /vntifkey="30"
                     /label=T7
     promoter        1365..1384
                     /note="T3"
     PCR_primer      complement(5815..5838)
                     /note="M13 FWD"
                     /current=0
     PCR_primer      1308..1331
                     /note="M13 REV"
                     /current=0
     misc_feature    4465..4566
                     /dnas_title="2xMembraneLocalization"
                     /vntifkey="21"
                     /label=2xMembraneLocalization
     CDS             4567..4596
                     /note="GA linker"
                     /translation=""
     CDS             4597..5310
                     /note="Superfolder GFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
                     /note="is superfolder GFP a weak dimer?"
     source          1..7217
                     /dnas_title="pMTB_memGAsfEGFP"
     PCR_primer      1355..1374
                     /note="pMTBF1_Forward"
                     /current=0
     PCR_primer      complement(5290..5314)
                     /pair="pMTBF1_Reverse"
                     /primer="CTTATTTGTAGAGCTCATCCATGCC"
                     /current=0
     PCR_primer      1355..1374
                     /pair="pMTBF1_Forward"
                     /primer="CAAGCGCGCAATTAACCCTC"
                     /current=0
     primer          complement(5290..5314)
                     /note="pMTBF1_Reverse"
     PCR_primer      5318..5339
                     /pair="pMTBF2_Forward"
                     /primer="TCATACGTATCCGGAACTCCCT"
                     /current=0
     primer          5318..5339
                     /note="pMTBF2_Forward"
     PCR_primer      complement(782..801)
                     /pair="pMTBF2_Reverse"
                     /primer="CGCTTACCGGATACCTGTCC"
                     /current=0
     primer          complement(782..801)
                     /note="pMTBF2_Reverse"
     PCR_primer      5196..5215
                     /pair="inDropsTracerRT"
                     /primer="CCTGTCCACCCAGTCTGTTC"
                     /current=1
     primer          5196..5215
                     /note="inDropsTracerRT"
ORIGIN      
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1381 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatctgctg
     1441 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
     1501 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
     1561 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
     1621 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
     1681 agggcctagt cattacctat actcaacgtt tactctgaga aacttccata ttttagaggt
     1741 aaaatgctca gaattgtaaa aagtcttgtc aaaatacttg gaatttgcca aaaacaaaat
     1801 aaaagtcaca tcacctcatt gtccttggta tcagcaagca cttttaacac aatcagctgt
     1861 ttttgtttat aactcccact gaaggccatg tgcattactc ccaagttaca cagtaaaaag
     1921 tcacatgtag tttatcatga tcactgctgc tcaagcacag ctttatctgt tattcagtgt
     1981 ggcaatatct gaaatgtgtg caaaatatta tttattgtag ctgatcacca acactttcag
     2041 tgtttttttt ttctactttt aatgagaatt atgttcttag gatttatcct tataaacctc
     2101 tttaaaaaga aatgttatgt ctgcatagac acgacatgaa attatgaaca gaaaatttaa
     2161 ctatttaaga tcaagacttg acatataaca tttctaatga acattaaacc tgaggtaggc
     2221 ccacttgcaa aaatgtaact gctatcactc actttataca tcattgtgta acttgtacac
     2281 acaaaaatag tgaagcaatg tggcatggaa tgcaggtccc attcattcaa agttattaaa
     2341 gcactttctt aaagtgcatt actactgagc taagaccagc tagttgctca tacaacatac
     2401 tacaagtagt gtcatcctaa tttggataaa aaaaatgtcc attttataca tggaaaagta
     2461 ctcattagat cccccattgt ttgtattacg gtatttcgtg aacacaagag gtaaatgacc
     2521 tacagagctg ctgttgtgtt agattgaaaa cacaacacag gatcatggag gcatccatca
     2581 ttatgacgca ttctgccact tgtaactctg cacagacttt ttgaaaagtt taattgagtc
     2641 ttggcgttgt cgcagaaaaa tacgaaaaat tctgtgttaa aatttgcaaa taaaaattcc
     2701 agttttatag gaaatcgcca tggccttgtt ctttaaccag ttaagcttcc ccttctttca
     2761 ctctcaagtt gcaagaagca agtgtagcaa tgtgcacgcg acagccgggt gtgtgacgct
     2821 ggaccaatca gagcacagag ctccgaaagt ttacctttta tggctagagc cgggtatgtg
     2881 ccgtcatata aaagagcgcg cccagctttt cagcctcact ttgagctcct ccacacgcag
     2941 ctagtgcgga atatcatctg cttgtaaccc attctcttaa gtcgacaacc ccccaaaccc
     3001 aaggtgagtt ggttttacag tttttaagcg tttaattata gtttctatct attaatgtta
     3061 agtaaaatgt gtaaatggat aaatatgatt tgatgaagtc tttagacagt tcataacggt
     3121 ctctttgtag ttatctacta ggtttgattt ataagtggtg gttcttttga taatctatgt
     3181 tacatttaat aaatattggc tgtatcttaa cttttttgta gcgttagtgg atgtggcagg
     3241 tgagaatgtc ggtaatttaa cgtgaccagt cgtaggcacg acattgaaca ttgaataggc
     3301 cggtgtgaaa tgggtgttaa gtcttaatat aaagttgtgt tctggctaat ctctctttaa
     3361 cagccataaa tgtgattttt aactacagtt ttgagggtgc tggtgagcat gttgcacact
     3421 tgatggatag ccggcatggg aagttctttg tgcaggcagt gctgcagcag ggtgtgacct
     3481 actttagcta gccggctaac cagctctcat ctgatgtaac gtaaacccca tcaatctaac
     3541 atcggctcat tcgtgcttta taagatcgaa gatcttgtta ttagtaacgg gtattggcat
     3601 ttagttttaa cttggttaat tttctttttg ttatgtgttc tatttaaatg tagcctgcag
     3661 ccccctctgt ctttaatgaa gctgaagatt aacgttaacg ttagtgaagt gttgtatccg
     3721 ctatgaaaga tttgaatttt agaggtgtgg tccactttaa taacggctgc ggagttctct
     3781 ggctgcactt cagccgctgt gactgtcgaa gcagagccca tcagtttttc aggatctcgg
     3841 tgatttttga ggctagacta tgaactgaac cgactgagag tgaccgtcca gaaatgcttt
     3901 agtgtgaagt ctccgctcac gaggagtccg tggactgact gcagatctgt gacgcagtaa
     3961 cacccggcgg cagacacccg ttgaaatgcg gttgtgtaat tgatcgcagg cgagggtgga
     4021 agaggatgtg aaacttcatt tgtgtaaaat ttagggagtg gtcctggtgt gatgaatgtc
     4081 gaaatctgtt cctttttact gagccctacg actctggctg agtgccacac cgccggcagc
     4141 cgcaaagcgg ctcaaaccat tgccttttat ggtaataatg agagaatgca gagggacttc
     4201 ctttgtctgg catatctgag gcgcgcgctg tcactagcgc ccaccagcgg tcagactgta
     4261 gaatgcagag caaaacagga agttgactcc agatggtcac atgcttgctg aaacgcctct
     4321 ccctggcagc agtgcaattc taacagtttc cctttctttt acagttcagc catcctaggc
     4381 ctatttaggt gacactatag aagagtacta atacgactca ctatagggag aagctacttg
     4441 ttctttttgc aGGATCCcgc caccatgggc tgcatcaaga gcaagcgcaa ggacaacctg
     4501 aacgacgacg aggccgccat gggctgcatc aagagcaagc gcaaggacaa cctgaacgac
     4561 gacgaggGAG CGCCAGCTGG AGGTGCAGGT GCAGCAATGT CTAAAGGTGA AGAACTGTTC
     4621 ACCGGTGTTG TTCCGATCCT GGTTGAACTG GATGGTGATG TTAACGGCCA CAAATTCTCT
     4681 GTTCGTGGTG AAGGTGAAGG TGATGCAACC AACGGTAAAC TGACCCTGAA ATTCATCTGC
     4741 ACTACCGGTA AACTGCCGGT TCCATGGCCG ACTCTGGTGA CTACCCTGAC CTATGGTGTT
     4801 CAGTGTTTTT CTCGTTACCC GGATCACATG AAGCAGCATG ATTTCTTCAA ATCTGCAATG
     4861 CCGGAAGGTT ATGTACAGGA GCGCACCATT TCTTTCAAAG ACGATGGCAC CTACAAAACC
     4921 CGTGCAGAGG TTAAATTTGA AGGTGATACT CTGGTGAACC GTATTGAACT GAAAGGCATT
     4981 GATTTCAAAG AGGACGGCAA CATCCTGGGC CACAAACTGG AATATAACTT CAACTCCCAT
     5041 AACGTTTACA TCACCGCAGA CAAACAGAAG AACGGTATCA AAGCTAACTT CAAAATTCGC
     5101 CATAACGTTG AAGACGGTAG CGTACAGCTG GCGGACCACT ACCAGCAGAA CACTCCGATC
     5161 GGTGATGGTC CGGTTCTGCT GCCGGATAAC CACTACCTGT CCACCCAGTC TGTTCTGTCC
     5221 AAAGACCCGA ACGAAAAGCG CGACCACATG GTGCTGCTGG AGTTCGTTAC TGCAGCAGGT
     5281 ATCACGCACG GCATGGATGA GCTCTACAAA taaGAATTCa tacgtatccg gaaCTCCCTA
     5341 TAGTGAGTCG TATTAccggt gatccagaca tgataagata cattgatgag tttggacaaa
     5401 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     5461 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     5521 tgtttcaggt tcagggggag gtgtgggagg ttttttgata tcccgggttt aaacgcatgc
     5581 agatctaata ctcaagtaca attttaatgg agtacttttt tacttttact caagtaagat
     5641 tctagccaga tacttttact tttaattgag taaaattttc cctaagtact tgtactttca
     5701 cttgagtaaa atttttgagt actttttaca cctctgtcaa gaaccatatg CCggtaccca
     5761 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5821 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5881 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5941 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6001 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6061 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6121 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6181 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6241 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6301 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6361 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6421 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6481 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6541 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6601 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6661 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6721 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6781 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6841 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6901 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6961 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7021 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7081 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7141 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7201 ggcccttccg gctggct
//

