LOCUS       Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1       13569 bp    DNA     circular SYN  1-JUN-2018
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2017Äê2ÔÂ17ÈÕ from SnapGene Viewer 2.6.2 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12314089|
COMMENT     VNTDBDATE|-12314089|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT         "BsmBI" "BsrGI" "DpnI" "DraI" "EaeI" "EagI" "EcoRV" "HpaI" "KpnI" 
COMMENT         "MfeI" "MluI" "NdeI" "NheI" "NotI" "PacI" "PmeI" "PmlI" "PvuI" "PvuII" 
COMMENT         "RsrII" "SacI" "SacII" "SalI" "ScaI" "SfiI" "SnaBI" "SpeI" "SphI" 
COMMENT         "StuI" "XbaI" "XhoI" "AgeI") (CStringList "atg") 
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COMMENT                            "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                            1 5 "TGATCA" "ACTAGT") 
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COMMENT                             "BglII: 6 sites") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                            "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                                      #495=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 14) 1 2 2 0 
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COMMENT                                      #496=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 15) 1 2 2 0 
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COMMENT                                      #497=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 16) 1 2 2 0 
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COMMENT                                      #498=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 17) 1 2 2 0 
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COMMENT                                      #499=(CLinePar 
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COMMENT                             "EaeI: 18 sites") 1 5 "YGGCCR" "RCCGGY") 
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COMMENT                                      #503=(CLinePar 
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COMMENT                                            " N2: 1902 " 1) 
COMMENT                                      #504=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #21# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3288 " 1) 
COMMENT                                      #505=(CLinePar 
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COMMENT                                            " N4: 4300 " 1) 
COMMENT                                      #506=(CLinePar 
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COMMENT                                            " N5: 7176 " 1) 
COMMENT                                      #507=(CLinePar 
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COMMENT                                      #508=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #21# 7) 1 2 2 0 191) 
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COMMENT                            1 5 "CGGCCG" "GCCGGC") 
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COMMENT                             (CGroupPar (CParagraph 140897632 (8 0) 1 1 1 0 178) 
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COMMENT                                      #514=(CLinePar 
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COMMENT                             "EcoRI: 4 sites") 1 5 "GAATTC" "CTTAAG") 
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COMMENT                                            " N1: 4566 " 1) 
COMMENT                                      #518=(CLinePar 
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COMMENT                             "EcoRV: 2 sites") 3 3 "GATATC" "CTATAG") 
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COMMENT                                      #523=(CLinePar 
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COMMENT                                            " N3: 7284 " 1) 
COMMENT                                      #524=(CLinePar 
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COMMENT                                            " N4: 7793 " 1) 
COMMENT                                      #525=(CLinePar 
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COMMENT                                            " N5: 9626 " 1) 
COMMENT                                      #526=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 10013 " 1) 
COMMENT                                      #527=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 10379 " 1) 
COMMENT                                      #528=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 8) 1 2 2 0 191) 
COMMENT                                            " N8: 11320 " 1))))) 
COMMENT                             "HindIII: 8 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                            3 3 "GTTAAC" "CAATTG") 
COMMENT                      #532=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899360 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                      #535=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #24# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 10810 " 1))))) "KpnI: 2 sites") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #536=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899168 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #25# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1777 " 1) 
COMMENT                                      #539=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #25# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2007 " 1))))) "MfeI: 2 sites") 
COMMENT                            1 5 "CAATTG" "GTTAAC") 
COMMENT                      #540=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140898656 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                            1 5 "ACGCGT" "TGCGCA") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140898272 (8 0) 1 1 1 0 178) 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3926 " 1) 
COMMENT                                      #546=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5041 " 1) 
COMMENT                                      #547=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6514 " 1) 
COMMENT                                      #548=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 10801 " 1) 
COMMENT                                      #549=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 10918 " 1))))) "NcoI: 5 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #550=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899840 (8 0) 1 1 1 0 178) 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 1947 " 1) 
COMMENT                                      #553=(CLinePar 
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COMMENT                                            " N2: 4054 " 1) 
COMMENT                                      #554=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 10323 " 1))))) "NdeI: 3 sites") 
COMMENT                            2 4 "CATATG" "GTATAC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899872 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                            1 5 "GCTAGC" "CGATCG") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140900416 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 4300 " 1) 
COMMENT                                      #561=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 2) 1 2 2 0 191) 
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COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140900192 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                            "AATTAATT") 
COMMENT                      #563=(CRSFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                            4 4 "GTTTAAAC" "CAAATTTG") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140900256 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #32# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2397 " 1) 
COMMENT                                      #569=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #32# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 8014 " 1))))) "PmlI: 2 sites") 
COMMENT                            3 3 "CACGTG" "GTGCAC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140898112 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2543 " 1) 
COMMENT                                      #573=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4780 " 1) 
COMMENT                                      #574=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 5418 " 1) 
COMMENT                                      #575=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5573 " 1) 
COMMENT                                      #576=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 6842 " 1) 
COMMENT                                      #577=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7146 " 1) 
COMMENT                                      #578=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 8358 " 1) 
COMMENT                                      #579=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 8) 1 2 2 0 191) 
COMMENT                                            " N8: 8592 " 1) 
COMMENT                                      #580=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 9) 1 2 2 0 191) 
COMMENT                                            " N9: 10560 " 1))))) "PstI: 9 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899968 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CParagraph 0 (10 #33# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #33# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 504 " 1) 
COMMENT                                      #584=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #33# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 10689 " 1) 
COMMENT                                      #585=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #33# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 13028 " 1))))) "PvuI: 3 sites") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899744 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #587=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #34# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #34# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 533 " 1) 
COMMENT                                      #589=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #34# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7036 " 1) 
COMMENT                                      #590=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #34# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 8149 " 1) 
COMMENT                                      #591=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #34# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 11589 " 1))))) 
COMMENT                             "PvuII: 4 sites") 3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #592=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140900000 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #593=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #594=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 1) 1 2 2 0 191) 
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COMMENT                            2 5 "CGGWCCG" "GCCWGGC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899936 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #596=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #36# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #597=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4321 " 1) 
COMMENT                                      #598=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 9237 " 1) 
COMMENT                                      #599=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 11023 " 1) 
COMMENT                                      #600=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 11371 " 1))))) "SacI: 4 sites") 
COMMENT                            5 1 "GAGCTC" "CTCGAG") 
COMMENT                      #601=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140900384 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #602=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #37# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #603=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1033 " 1) 
COMMENT                                      #604=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2746 " 1) 
COMMENT                                      #605=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3274 " 1) 
COMMENT                                      #606=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4312 " 1) 
COMMENT                                      #607=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 10815 " 1) 
COMMENT                                      #608=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 10952 " 1) 
COMMENT                                      #609=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 11362 " 1))))) 
COMMENT                             "SacII: 7 sites") 4 2 "CCGCGG" "GGCGCC") 
COMMENT                      #610=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140900224 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #611=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #38# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #612=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 10259 " 1))))) "SalI: 1 site") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #613=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899712 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #614=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #39# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #615=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4440 " 1) 
COMMENT                                      #616=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6828 " 1) 
COMMENT                                      #617=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7203 " 1) 
COMMENT                                      #618=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7698 " 1) 
COMMENT                                      #619=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 9255 " 1) 
COMMENT                                      #620=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 10369 " 1) 
COMMENT                                      #621=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 10791 " 1) 
COMMENT                                      #622=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 8) 1 2 2 0 191) 
COMMENT                                            " N8: 11316 " 1) 
COMMENT                                      #623=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 9) 1 2 2 0 191) 
COMMENT                                            " N9: 13138 " 1))))) "ScaI: 9 sites") 
COMMENT                            3 3 "AGTACT" "TCATGA") 
COMMENT                      #624=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899776 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SfiI: 0 sites") 8 5 
COMMENT                            "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
COMMENT                      #625=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140898144 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #626=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #627=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1788 " 1) 
COMMENT                                      #628=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2547 " 1) 
COMMENT                                      #629=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 5092 " 1) 
COMMENT                                      #630=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 9435 " 1))))) "SmaI: 4 sites") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #631=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140899808 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #632=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #41# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #633=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3950 " 1))))) "SnaBI: 1 site") 
COMMENT                            3 3 "TACGTA" "ATGCAT") 
COMMENT                      #634=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 140900288 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #635=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #42# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #636=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #42# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4287 " 1))))) "SpeI: 1 site") 
COMMENT                            1 5 "ACTAGT" "TGATCA") 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #43# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1656 " 1) 
COMMENT                                      #640=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #43# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4847 " 1) 
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COMMENT                                            " N3: 5681 " 1))))) "SphI: 3 sites") 
COMMENT                            5 1 "GCATGC" "CGTACG") 
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COMMENT                                            " N1: 1150 " 1) 
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COMMENT                                            " N2: 2458 " 1) 
COMMENT                                      #646=(CLinePar 
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COMMENT                                            " N3: 11036 " 1))))) "StuI: 3 sites") 
COMMENT                            3 3 "AGGCCT" "TCCGGA") 
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COMMENT                             (CGroupPar (CParagraph 140901184 (8 0) 1 1 1 0 178) 
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COMMENT                                            " N1: 1419 " 1) 
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COMMENT                                            " N2: 2521 " 1) 
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COMMENT                                            " N3: 2671 " 1) 
COMMENT                                      #652=(CLinePar 
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COMMENT                                            " N4: 4293 " 1) 
COMMENT                                      #653=(CLinePar 
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COMMENT                                            " N5: 5898 " 1))))) "XbaI: 5 sites") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
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COMMENT                                            " N1: 672 " 1) 
COMMENT                                      #657=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #46# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 735 " 1) 
COMMENT                                      #658=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #46# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 813 " 1) 
COMMENT                                      #659=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #46# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7256 " 1))))) "XhoI: 4 sites") 
COMMENT                            1 5 "CTCGAG" "GAGCTC") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                            " N1: 1786 " 1) 
COMMENT                                      #663=(CLinePar 
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COMMENT                                            " N2: 2545 " 1) 
COMMENT                                      #664=(CLinePar 
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COMMENT                                            " N3: 5090 " 1) 
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COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map") 
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COMMENT                            (CGroupPar (CParagraph 0 (0 0) 1 2 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                     (CObjectList)) "Reading Frame 1 (0 ORFs)") 
COMMENT                              #669=(CFolderPar 
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COMMENT                              #670=(CFolderPar 
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COMMENT                            "Direct(Forward) strand") 
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COMMENT                             (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
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COMMENT                                            "4807(c1) (2038 codons)"))) 
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COMMENT                              #676=(CFolderPar 
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COMMENT                              #677=(CFolderPar 
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COMMENT                            "Complementary(Reverse) strand"))) 
COMMENT                    "Open Reading Frames") 
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COMMENT                             " 9544: Pblu-AAVS1-Cas9-p300baeImutant-M2rtTA-AAVS1: SphI(5681) - SphI(1656)" 
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COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
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COMMENT          #684=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #685=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
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COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #688=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #689=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #690=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #691=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #692=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #693=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #694=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #695=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #696=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #697=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
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COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #699=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
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COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #702=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #703=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #704=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #705=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #706=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #707=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #708=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #709=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #710=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #711=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #712=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #713=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #714=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #715=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #716=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #717=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
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COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
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COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #719=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #720=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 
COMMENT         -11850) 0 1 0 1 1 
COMMENT        (mapper: 22.8091 -54.6097 40.0378 40.0378 0.01 10 -1 13569 13569 1 0 0) 
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COMMENT              (CObjectList 
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COMMENT                     (CObjectList 
COMMENT                      #724=(CAxis 
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COMMENT                             6.27465 6.27265 1 0.0214037) 0.0462317) 
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COMMENT                            1.74233 0.833333 0 
COMMENT                            "Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1" "@N" 1 0 
COMMENT                            0.871165 0 -10 21.6545 1.44863 Nil) 
COMMENT                      #726=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #684# -1511 100) 
COMMENT                            (LOGPEN 141007016 141007256 386) -1511 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "13569 bp" "@L bp" 16 0 -0.871165 
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COMMENT               #727=(CGroupWidget (CWidget 13 (12 0) 1 2 0 0 Nil -4158 100) 
COMMENT                     (CObjectList 
COMMENT                      #728=(CGroupWidget 
COMMENT                            (CWidget 1 (13 1 0) 1 2 0 0 Nil -1019 100) 
COMMENT                            (CObjectList 
COMMENT                             #729=(CGroupWidget 
COMMENT                                   (CWidget 1 (14 -1 0) 1 2 0 0 Nil -3251 100) 
COMMENT                                   (CObjectList 
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COMMENT                                           0.835356 1.22468 4.05129) 0.2 
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COMMENT                     (CObjectList)) 
COMMENT               #731=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -531 100) 
COMMENT                     (CObjectList 
COMMENT                      #732=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -1721 100) 
COMMENT                            (CObjectList 
COMMENT                             #733=(CScratch 
COMMENT                                   (CWidget 0 (3 #59# 0) 1 2 0 0 #691# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.9246 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #734=(CLabel (CWidget 0 (0 0) 1 2 0 0 #683# 8 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T2A" "@N" 1 
COMMENT                                   6.5609 17.4233 0 -10 1.6984 1.17389 #733#) 
COMMENT                             #735=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 0.835356 5.61808 
COMMENT                                    5.89547 1 0.082322) 0.8 1.8 0) 
COMMENT                             #736=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "PuroR" "@N" 1 
COMMENT                                   8.79269 12.1963 0 -10 2.62252 1.17389 #735#) 
COMMENT                             #737=(CWideArrow 
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COMMENT                                    (LOGBRUSH 0 6724095 0) 1 5.10445 5.4498 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #738=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rtTA-Advanced" 
COMMENT                                   "@N" 1 15.0479 -10.454 0 -10 6.21912 1.17389 
COMMENT                                   #737#) 
COMMENT                             #739=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #81# 0) 1 2 0 0 #686# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 0.0577897 0.455845 
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COMMENT                             #740=(CLabel (CWidget 0 (0 0) 1 2 0 0 #683# 16 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -5.94242 47.0429 0 -10 2.54759 1.17389 #739#) 
COMMENT                             #741=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 1.27877 3.19138 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #742=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #683# 1376671997 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Cas9 wt" "@N" 1 
COMMENT                                   -13.2528 -17.4233 0 -10 3.32186 1.17389 #741#) 
COMMENT                             #743=(CWideArrow 
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COMMENT                             #744=(CLabel 
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COMMENT                                   "p300 baeI mutant" "@N" 1 11.0009 -52.2699 0 
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COMMENT                      #748=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -2628 100) 
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FEATURES             Location/Qualifiers
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /vntifkey="28"
                     /label=M13\fwd
                     /note="common sequencing primer, one of multiple similar variants"
     promoter        629..647
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             755..808
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /vntifkey="4"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides."
     CDS             818..1417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /vntifkey="4"
                     /label=PuroR
                     /note="confers resistance to puromycin"
     polyA_signal    1455..1679
                     /vntifkey="25"
                     /label=bGH\poly(A)\signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    1686..1767
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1782..2528)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /vntifkey="4"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline."
     promoter        complement(3647..3924)
                     /vntifkey="30"
                     /label=chicken\beta-actin\promoter
     enhancer        3926..4229
                     /vntifkey="9"
                     /label=CMV\enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(10954..10978)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /vntifkey="31"
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(10995..11309)
                     /vntifkey="30"
                     /label=tight\TRE\promoter
                     /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter"
     protein_bind    11069..11087
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /vntifkey="31"
                     /label=tet\operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11105..11123
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /vntifkey="31"
                     /label=tet\operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11140..11158
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /vntifkey="31"
                     /label=tet\operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11176..11194
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /vntifkey="31"
                     /label=tet\operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11212..11230
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /vntifkey="31"
                     /label=tet\operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11247..11265
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /vntifkey="31"
                     /label=tet\operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11283..11301
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /vntifkey="31"
                     /label=tet\operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(11384..11402)
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11423..11439)
                     /vntifkey="28"
                     /label=M13\rev
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    11447..11463
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=lac\operator
                     /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11471..11501)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11516..11537
                     /bound_moiety="E. coli catabolite activator protein"
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(11825..12413)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(12584..13444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(13445..13549)
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     misc_feature    11325..11347
                     /vntifkey="21"
                     /label=AAVS1
     misc_feature    677..699
                     /vntifkey="21"
                     /label=AAVS1
     CDS             6667..10803
                     /vntifkey="4"
                     /label=Cas9\wt
     CDS             4813..6663
                     /vntifkey="4"
                     /label=p300\baeI\mutant
     primer          4121..4141
                     /vntifkey="27"
                     /label=CAGR2
     primer          6009..6028
                     /vntifkey="27"
                     /label=p300baeImutant-F(R)
BASE COUNT     3059 a      3630 c      3395 g      3485 t 
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac 
      661 cgggcccccc ctcgaggtca ccaatcctgt ccctagtgga agcttctgac ctcttctctt 
      721 cctcccacag ggcctcgaga gatctggcag cggagagggc agaggaagtc ttctaacatg 
      781 cggtgacgtg gaggagaatc ccggccctag gctcgagatg accgagtaca agcccacggt 
      841 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 
      901 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 
      961 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 
     1021 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 
     1081 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 
     1141 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 
     1201 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 
     1261 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 
     1321 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 
     1381 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct agagggcccg tttaaacccg 
     1441 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 
     1501 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 
     1561 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 
     1621 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg 
     1681 tcgaggcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 
     1741 accattataa gctgcaataa acaagttaac aacaacaatt gttacccggg gagcatgtca 
     1801 aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc gtcaagggca 
     1861 tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggccggccc gccgctttcg 
     1921 cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt 
     1981 tcggctccct gccggtcgaa cagctcaatt gcttgtctca gaagtggggg catagaatcg 
     2041 gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag 
     2101 cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct 
     2161 tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg 
     2221 gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg gaatgacttg 
     2281 gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagaa gtgggtatga 
     2341 tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc 
     2401 cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct 
     2461 tcgataccga ctccattgag taattccaga gcgccgttta tgactttgct cttgtccagt 
     2521 ctagacatgg tgaattcctg cagcccgggg gatccactag cttcgatcaa gccgaattct 
     2581 ttgccaaaat gatgagacag cacaataacc agcacgttgc ccaggagctg taggaaaaag 
     2641 aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg ccagaagccg 
     2701 aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cccgcggaca gccccgaggc 
     2761 tggagaggga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc gcccatttcc 
     2821 ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag agggggtgcg gcggcgcctc 
     2881 ccagatttcg gctccgcaca gatttgggac aaaggaagtc cctgcgccct ctcgcacgat 
     2941 taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agccgccggc gctccggggg 
     3001 ccgccgcgcc cctcccccga gccctccccg gcccgaggcg gccccgcccc gcccggcacc 
     3061 cccacctgcc gccacccccc gcccggcacg gcgagccccg cgccacgccc cgtacggagc 
     3121 cccgcacccg aagccgggcc gtgctcagca actcggggag gggggtgcag ggggggttgc 
     3181 agcccgaccg acgcgcccac accccctgct caccccccca cgcacacacc ccgcacgcag 
     3241 cctttgttcc cctcgcagcc ccccccgcac cgcggggcac cgcccccggc cgcgctcccc 
     3301 tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc cgcagcgctc acagccgccg 
     3361 ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac acacgcacgc accccccgag 
     3421 ccgctccccc cgcacaaagg gccctcccgg agcccctcaa ggctttcacg cagccacaga 
     3481 aaagaaacaa gccgtcatta aaccaagcgc taattacagc ccggaggaga agggccgtcc 
     3541 cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg 
     3601 gcggcggagc ggggcacggg gcgaaggcag cgtcgcagcg actccccgcc cgccgcgcgc 
     3661 ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg 
     3721 cgccgctctg attggctgcc gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc 
     3781 gccccgcccc gcctggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata 
     3841 attaaaaaat aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga 
     3901 agcagaacgt ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact 
     3961 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac 
     4021 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt 
     4081 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta 
     4141 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag 
     4201 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg 
     4261 accccgtaat tgattactat taataactag ttctagagcg gccgccaccg cggtggagct 
     4321 cccagcttct gatggaatta gaacttggca aaacaatact gagaatgaag tgtatgtgga 
     4381 acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga aaccacagta 
     4441 cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag atctatagat 
     4501 ctatagatca tgagtgggag gaatgagctg gcccttaatt tggttttgct tgtttaaatt 
     4561 atgatatcca actatgaaac attatcataa agcaatagta aagagccttc agtaaagagc 
     4621 aggcatttat ctaatcccac cccaccccca cccccgtagc tccaatcctt ccattcaaaa 
     4681 tgtaggtact ctgttctcac ccttcttaac aaagtatgac aggaaaaact tccattttag 
     4741 tggacatctt tattgtttaa tagatcatca atttctgcag acttacagcg gatccccacg 
     4801 cgtttaaccg gtgtcctggc tctgcgtgtg cagctccacc agcatgcaca tggtggacca 
     4861 ctgggctctt cggagtgaag agaactccag gtgcttgtcc cttgccagcg tgagaaacgc 
     4921 atcccgacca tccatcagat cgcaggggat gagaggatca ggatcaacaa tgggaggcag 
     4981 ggagttggca gcagggccag caatgaggcg gatcacaaag aagacctctt tatgcttctc 
     5041 catggtggca tatagtttct gtgagaggtc gttagataca ttgggcatcc cgggtttctt 
     5101 cttgttgccc ctactcaggc tgctcttatt tttgctggtt ttcttattat tcttcttttt 
     5161 agcatttttg ctgtctccct tggtcacatc tgtgctttca ttgctggtgt tttcctctcg 
     5221 ttttctctct tcttcctcct gttccagttc cttaatgctt tcttccagaa cattgggcca 
     5281 gaaatcaccc tcgaaataag gcaattcctt tgcacttgtt aatctatctt cagtagcttg 
     5341 tttaaaaata tccttgtagt catggacaat acgctctgat acagccttgt caagcatttt 
     5401 tttgtaccat tcctgcagtc gcttgggctt gggtatcttc tggtcaggag gatggcaatg 
     5461 gaagatataa tcatctccct cacttggtgg acatgcccaa atatgccctg ttgtgtaacc 
     5521 taatttcttg acatattcta aatatccaat taggatttca tgatagactg cagtcctcaa 
     5581 gcatttagga cggaagaaat gaacactatc gaggtaagat atgtatactc tcctctggtt 
     5641 gggtggaggg cagtcagagc catactcttg aacatgcatg ccaaagaagc acaggtcaac 
     5701 accatcaatt tcttcaaagg caaagagggc tttggttcgg tatggaaagg attctgccat 
     5761 ctctccactg tccacaaacc ttgctttcat gcctggtttt acttccacgg ttttgtcaga 
     5821 agcatgaact actctaacag tgacctctcc tgactcaggg tgattctgtc gcctcagaaa 
     5881 gtcattcaca cgattctcta gaaaggtgcc aagtctggta gatggcaacc ttttagcaga 
     5941 aaacttattt tctttcctag ttcgtgcact tttctttaaa cagccatcac agacgaatcc 
     6001 agcaggccag atgatctcat ggtgaaggac acagatctga tgcatctttc ttccgcactc 
     6061 tgtacattca acaaacagtt caggatccag tgtgtcattt tttctcttgg aaaattgttc 
     6121 tttatttatt gtagtttgag gctgggaagg gtcatccccc aaagaaacgc tctccccttg 
     6181 gatctcattg aaacacttct cacagaaatg atacctgttc tggtaactgt aataagtggc 
     6241 atcacgaggt attgtgcaca actgtttgcc gtagcaacac agtgtctgtg gagagaactc 
     6301 caactttctg ccgcaacaat atccaaggct ttgcatcact gggtcaattt cttgttcaaa 
     6361 gacctcagag agcttggagc agtatttgta tacccgtgat gttttccggt tatataacca 
     6421 ggcattattg aacataagcc aaatatcatc gacatactgc cagggctcct gatactgtcc 
     6481 agtgtctaac ttcctcttaa tggtagaaag atccatgggg ctcttcacaa tatcaaagta 
     6541 atcagggatt cctaaaagct gagggtccac aggttgacga aagggaaggg attctggatc 
     6601 ctgacggtaa agtgcctcca aagttggcat cagtgcctgt cgtagttctt ctggtttgaa 
     6661 aatgctcacc ttcctcttct tcttggggtc agccctgctg tctccaccga gctgagagag 
     6721 gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac 
     6781 ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc 
     6841 aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat 
     6901 gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag 
     6961 gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg 
     7021 ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga gcttttcata 
     7081 gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt 
     7141 ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct caaagagaga 
     7201 gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt tcgcctcgag 
     7261 aaagtcgatg gggttttttt cgaagcttga tcgctccatg attgtgatgc ccagcagttc 
     7321 cttgacgctt ttgagttttt tagacttccc tttctccact ttggccacaa ccagtacact 
     7381 gtaagcgact gtaggagaat cgaatccgcc gtatttcttg gggtcccaat cttttttgcg 
     7441 tgcgatcagc ttgtcgctgt tccttttcgg gaggatactt tccttggaga agcctccggt 
     7501 ctgtacttcg gtctttttaa cgatgttcac ctgcggcatg gacaggacct tccggactgt 
     7561 cgcgaaatcc ctacccttgt cccacacgat ttctcctgtt tctccgtttg tttcgataag 
     7621 tggtcgcttc cgaatctctc cattggccag tgtaatctcg gtcttgaaaa aattcataat 
     7681 attgctgtaa aagaagtact tagcggtggc cttgcctatt tcctgctcag actttgcgat 
     7741 cattttccta acatcgtaca ctttatagtc tccgtaaaca aattcagatt caagcttggg 
     7801 atattttttg ataagtgcag tgcctaccac tgcattcagg taggcatcat gcgcatggtg 
     7861 gtaattgttg atctctctca ccttataaaa ctgaaagtcc tttctgaaat ctgagaccag 
     7921 cttagacttc agagtaataa ctttcacctc tcgaatcagt ttgtcatttt catcgtactt 
     7981 ggtgttcatg cgtgaatcga gaatttgggc cacgtgcttg gtgatctggc gtgtctcaac 
     8041 aagctgcctt ttgatgaagc cggctttatc caactcagac aggccacctc gttcagcctt 
     8101 agtcagatta tcgaacttcc gttgtgtgat cagtttggcg ttcagcagct gccgccaata 
     8161 atttttcatt ttcttgacaa cttcttctga ggggacgtta tcactcttcc ctctattttt 
     8221 atcggatctt gtcaacactt tattatcaat agaatcatct ttgagaaaag actggggcac 
     8281 gatatgatcc acgtcgtagt cggagagccg attgatgtcc agttcctgat ccacgtacat 
     8341 gtccctgccg ttctgcaggt agtacaggta gagcttctca ttctgaagct gggtgttttc 
     8401 aactgggtgt tccttaagga tttgggaccc cagttctttt ataccctctt caatcctctt 
     8461 catcctttcc ctactgttct tctgtccctt ctgggtagtt tggttctctc gggccatctc 
     8521 gataacgata ttctcgggct tatgccttcc cattactttg acgagttcat ccacgacctt 
     8581 aacggtctgc agtattccct ttttgatagc tgggctacct gcaagattag cgatgtgctc 
     8641 gtgaagactg tccccctggc cagaaacttg tgctttctgg atgtcctcct taaaggtgag 
     8701 agagtcatca tggatcaact gcatgaagtt ccggttggca aatccatcgg acttaagaaa 
     8761 atccaggatt gtctttccac tctgcttgtc tcggatccca ttgatcagtt ttcttgacag 
     8821 ccgcccccat cctgtatatc ggcgcctctt gagctgtttc atgactttgt cgtcgaagag 
     8881 atgagcgtaa gttttcaagc gttcttcaat catctcccta tcttcaaaca acgtaagggt 
     8941 gaggacaatg tcctcaagaa tgtcctcgtt ctcctcattg tccaggaagt ccttgtcttt 
     9001 aatgattttc aggagatcgt gatacgttcc cagggatgcg ttgaagcgat cctccactcc 
     9061 gctgatttca acagagtcga aacattcaat ctttttgaaa tagtcttctt tgagctgttt 
     9121 cacggtaact ttccggttcg tcttgaagag gaggtccacg atagctttct tctgctctcc 
     9181 agacaggaat gctggctttc tcatcccttc tgtgacgtat ttgaccttgg tgagctcgtt 
     9241 ataaactgtg aagtactcgt acagcagaga gtgtttagga agcacctttt cgttaggcag 
     9301 atttttatca aagttagtca tcctttcgat gaaggactgg gcagaggccc ccttatccac 
     9361 gacttcctcg aagttccagg gagtgatggt ctcttctgat ttgcgagtca tccacgcgaa 
     9421 tctggaattt ccccgggcga gggggcctac atagtagggt atccgaaatg tgaggatttt 
     9481 ctcaatcttt tccctgttat ctttcaaaaa ggggtagaaa tcctcttgcc gcctgaggat 
     9541 agcgtgcagt tcgcccaggt gaatctggtg ggggatgctt ccattgtcga aagtgcgctg 
     9601 tttgcgcaac agatcttctc tgttaagctt taccagcagc tcctcggtgc cgtccatttt 
     9661 ttccaagatg ggcttaataa atttgtaaaa ttcctcctgg cttgctccgc cgtcaatgta 
     9721 tccggcgtag ccatttttag actgatcgaa gaaaatttcc ttgtacttct caggcagttg 
     9781 ctgtctgaca agggccttca gcaaagtcaa gtcttggtgg tgctcatcat agcgcttgat 
     9841 catactagcg ctcagcggag ctttggtgat ctccgtgttc actcgcagaa tatcactcag 
     9901 cagaatggcg tctgacaggt tctttgccgc caaaaaaagg tctgcgtact ggtcgccgat 
     9961 ctgggccagc agattgtcga gatcatcatc gtaggtgtct ttgctcagtt gaagcttggc 
    10021 atcttcggcc aggtcgaagt tagatttaaa gttgggggtc agcccgagtg acagggcgat 
    10081 aagattacca aacaggccgt tcttcttctc cccagggagc tgtgcgatga ggttttcgag 
    10141 ccgccgggat ttggacagcc tagcgctcag gattgctttg gcgtcaactc cggatgcgtt 
    10201 gatcgggttc tcttcgaaaa gctgattgta agtctgaacc agttggataa agagtttgtc 
    10261 gacatcgctg ttgtctgggt tcaggtcccc ctcgatgagg aagtgtcccc gaaatttgat 
    10321 catatgcgcc agcgcgagat agatcaaccg caagtcagcc ttatcagtac tgtctacaag 
    10381 cttcttcctc agatgatata tggttgggta cttttcatgg tacgccacct cgtccacgat 
    10441 attgccaaag attgggtggc gctcgtgctt tttatcctcc tccaccaaaa aggactcctc 
    10501 cagcctatgg aagaaagagt catccacctt agccatctca ttactaaaga tctcctgcag 
    10561 gtagcagatc cgattctttc tgcgggtata tctgcgccgt gctgttcttt tgagccgcgt 
    10621 ggcttcggcc gtctccccgg agtcgaacag gagggcgcca atgaggttct tctttatgct 
    10681 gtggcgatcg gtattgccca gaactttgaa ttttttgctc ggcaccttgt actcgtccgt 
    10741 aatgacggcc cagccgacgc tgtttgtgcc gatatcgagc ccaatggagt acttcttgtc 
    10801 catgggtacc ccgcggtgaa tgcccacctt cctcttcttc ttgggggcca tcttgtcatc 
    10861 gtcatccttg taatcgatgt catgatcttt ataatcaccg tcatggtctt tgtagtccat 
    10921 ggtgctagct ccggtccaac ctgaaaaccg cggagcctgc ttttttgtac aaacttgttg 
    10981 atcggaattc tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc 
    11041 caccgtacac gcctacctcg acatacgttc tctatcactg atagggagta aactcgacat 
    11101 acgttctcta tcactgatag ggataaactc gacatacgtt ctctatcact gatagggagt 
    11161 aaactcgaca tacgttctct atcactgata gggagtaaac tcgacatacg ttctctatca 
    11221 ctgataggga gtaaactcga catcgttctc tatcactgat agggagtaaa ctcgacatac 
    11281 gttctctatc actgataggg agtaaactcg acagtactaa gcttgtcacc aatcctgtcc 
    11341 ctagtgggcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa 
    11401 tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
    11461 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 
    11521 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 
    11581 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
    11641 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
    11701 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
    11761 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
    11821 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
    11881 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
    11941 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
    12001 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
    12061 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
    12121 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
    12181 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
    12241 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
    12301 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
    12361 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
    12421 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
    12481 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
    12541 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
    12601 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
    12661 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
    12721 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
    12781 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
    12841 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
    12901 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
    12961 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
    13021 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
    13081 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
    13141 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
    13201 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
    13261 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
    13321 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
    13381 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
    13441 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
    13501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
    13561 gaaaagtgc 
//
