LOCUS       Exported                4762 bp ds-DNA     circular SYN 01-JUN-2018
DEFINITION  .
ACCESSION   CMV\promoter-mCh-CAAX
VERSION     .
KEYWORDS    ITRACK acceptor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
            ORIGDB|GenBank
            VNTDATE|616858489|
            VNTDBDATE|616858489|
            LSOWNER|
            VNTAUTHORNAME|Seok Jin Lee|
            VNTAUTHOREML|sjlee8@gmail.com|
            VNTOAUTHORNAME|Hideji Murakoshi|
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="synthetic DNA construct"
                     /label=dnas_title=promoter-mCh-CAAX
                     /note="dnas_title=promoter-mCh-CAAX"
                     /note="color: #ffffff"
     promoter        1..589
                     /label=CMV E promotor
                     /note="dnas_title=CMV E promotor
                     vntifkey=29"
                     /note="color: #ffffff"
     primer_bind     601..633
                     /label=Primer 1
                     /note="color: black; sequence: 
                     CCGGTGGATCCCATGGTGAGCAAGGGCGAGGAG"
     primer_bind     611..633
                     /label=Primer 3
                     /note="color: black; sequence: 
                     CGGTGGATCCCATGGTGAGCAAGGGCGAGGAG"
     misc_feature    613..1333
                     /label=mCh cut
                     /note="dnas_title=mCh cut
                     vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     complement(1189..1208)
                     /label=Primer 6
                     /note="color: black; sequence: ttgtaggcgccgggcagctg"
     primer_bind     1209..1233
                     /label=Primer 5
                     /note="color: black; sequence: cgtcaacaccaagttggacatcacc"
     primer_bind     complement(1301..1321)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     TCGAGATGGTACCCCTCACTTGTACAGCTCGTCCATGC"
     primer_bind     complement(1301..1321)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     GAGATGGTACCCCTCACTTGTACAGCTCGTCCATGC"
     misc_feature    1363..1413
                     /label=K-Ras lipid
                     /note="dnas_title=K-Ras lipid
                     vntifkey=21"
                     /note="color: #a6acb3"
     rep_origin      1678..2133
                     /label=f1 ori
                     /note="dnas_title=f1 ori
                     vntifkey=33"
                     /note="color: #ffff00"
     promoter        2195..2230
                     /label=Bac promotor of Kan gene
                     /note="dnas_title=Bac promotor of Kan gene
                     vntifkey=29"
                     /note="color: #ffffff"
     promoter        2307..2575
                     /label=SV40 E promotor
                     /note="dnas_title=SV40 E promotor
                     vntifkey=29"
                     /note="color: #ffffff"
     promoter        2658..3186
                     /label=Kanamycin
                     /note="dnas_title=Kanamycin
                     vntifkey=29"
                     /note="color: #ffffff"
     polyA_signal    3688..3706
                     /label=HSV TK PolyA
                     /note="dnas_title=HSV TK PolyA
                     vntifkey=25"
                     /note="color: #a6acb3"
     rep_origin      4037..4680
                     /label=pUC ori
                     /note="dnas_title=pUC ori
                     vntifkey=33"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggacaaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg agcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtaa aaagaagaaa
     1381 aagaagtcaa agacaaagtg tgtaattatg taagggcccg ggatccaccg gatctagata
     1441 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     1501 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     1561 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1621 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
     1681 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     1741 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     1801 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     1861 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     1921 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     1981 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     2041 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     2101 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     2161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     2281 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2341 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2401 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     2461 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2521 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     2581 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     2641 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2701 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2761 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     2821 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     2881 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     2941 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3001 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3061 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3121 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3181 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3241 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3301 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3361 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3421 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3481 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3541 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3601 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     3661 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     3721 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     3781 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     3841 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     3901 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3961 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4201 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4441 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4681 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4741 taaccgtatt accgccatgc at
//
