LOCUS Exported 5484 bp ds-DNA circular SYN 01-JUN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5484) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 1, 2018 from SnapGene 4.1.9 http://www.snapgene.com COMMENT ID HM01-77\\mCherry-C1 standard; circular DNA; ; 4722 BP. DT 8-OCT-2006 CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|425908000| CC VNTDBDATE|425908000| CC LSOWNER| CC VNTAUTHORNAME|Hideji Murakoshi| FEATURES Location/Qualifiers source 1..5484 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..589 /label=CMV E promotor CDS 613..1320 /label=mCherry primer_bind 613..637 /label=Set 1 - Pair 1 - TCTCGAGCTCAAGCTATGGTGAGCAAGGGCGAG primer_bind complement(1327..1347) /label=Set 1 - Pair 1 - AGCTTGAGCTCGAGATCTGAG primer_bind complement(1327..1347) /label=AGCTTGAGCTCGAGATCTGAG primer_bind 1333..1372 /label=Set 1 - Pair 1 - TCTCGAGCTCAAGCTATGGTGAGCAAGGGCGAG CDS 1348..2055 /label=mCherry misc_feature 2053..2106 /label=PKC inhibitor (18aa) primer_bind complement(2085..2124) /label=GACTGCAGAATTCGATCAGTTCTTGACTTCGTGAACGTTC primer_bind 2110..2130 /label=Set 1 - Pair 1 - TCGAATTCTGCAGTCGACGGT primer_bind 2110..2130 /label=TCGAATTCTGCAGTCGACGGT polyA_signal 2303..2353 /label=SV40 Early PolyA rep_origin 2400..2855 /direction=RIGHT /label=f1 ori promoter 2917..2952 /label=Bac promotor of Kan gene promoter 3029..3297 /label=SV40 E promotor promoter 3380..3908 /label=Kanamycin polyA_signal 4410..4428 /label=HSV TK PolyA rep_origin 4759..5402 /direction=RIGHT /label=pUC ori ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tcaagctatg gtgagcaagg gcgaggagga taacatggcc 1381 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 1441 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 1501 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 1561 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 1621 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 1681 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 1741 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 1801 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 1861 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 1921 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 1981 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 2041 gacgagctgt acagattcgc tcgtaaaggt gctcttcgtc agaagaacgt tcacgaagtc 2101 aagaactgat cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga 2161 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 2221 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 2281 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 2341 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 2401 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 2461 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 2521 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 2581 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 2641 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 2701 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 2761 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 2821 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 2881 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2941 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 3001 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 3061 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 3301 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 3361 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 3421 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 3481 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 3541 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 3601 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 3661 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 3721 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 3781 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 3841 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 3901 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 3961 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 4021 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 4081 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 4141 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 4201 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 4261 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 4321 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 4381 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 4441 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 4501 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 4561 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 4621 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 4681 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 4741 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 4801 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 4861 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 4921 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 4981 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 5041 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 5101 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 5161 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 5221 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 5281 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 5341 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 5401 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 5461 gataaccgta ttaccgccat gcat //