LOCUS       Exported                8815 bp ds-DNA     circular SYN 05-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 5, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..151
     misc_signal     5734..5859
                     /label=CEN6 (centromere) sequence
                     /note="CEN6 (centromere) sequence"
     rep_origin      6057..6163
                     /direction=RIGHT
                     /label=H4 Autonomous Replcating Sequence
                     /note="H4 Autonomous Replcating Sequence"
ORIGIN
        1 gtcgacctcg aggcatgtgc tctgtatgta tataaaactc ttgttttctt cttttctcta
       61 aatattcttt ccttatacat taggtccttt gtagcataaa ttactatact tctatagaca
      121 cgcaaacaca aatacacaca ctaaattaat aatgaccgga tccggagctt ggctgttgcc
      181 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
      241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactta atcgccttgc
      301 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      361 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc
      421 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc
      481 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac
      541 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa
      601 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc
      661 ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc
      721 gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt
      781 gctgcgttgg agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat
      841 tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc
      901 cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg
      961 cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc
     1021 cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg
     1081 cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct
     1141 ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg
     1201 cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc
     1261 gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga
     1321 tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg
     1381 ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta
     1441 tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga
     1501 tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg
     1561 taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca
     1621 cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg
     1681 cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga
     1741 agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg
     1801 agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg
     1861 tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg
     1921 ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta
     1981 cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt
     2041 tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca
     2101 gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga
     2161 taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt
     2221 gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc
     2281 ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg
     2341 gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt
     2401 gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg
     2461 catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat
     2521 gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc
     2581 gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga
     2641 acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga
     2701 tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac
     2761 cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt
     2821 tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct
     2881 ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga
     2941 ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc
     3001 gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc
     3061 acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat
     3121 ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg
     3181 tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattaca
     3241 gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaatacc gggcaggcca
     3301 tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa cacaaacttt
     3361 tggatgttcg gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt
     3421 ttcccgattt ggctacatga catcaaccat atcagcaaaa gtgatacggg tattattttt
     3481 gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa
     3541 ctcggcctcg actctagcga attaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     3601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4381 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5701 gcgcacattt ccccgaaaag tgccacctgg gtccttttca tcacgtgcta taaaaataat
     5761 tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat
     5821 taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca
     5881 aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct
     5941 tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat
     6001 cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct
     6061 ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta
     6121 ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt
     6181 aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg
     6241 atccgtccta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     6301 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6361 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6421 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     6481 tactgagagt gcaccataaa cgacattact atatatataa tataggaagc atttaataga
     6541 cagcatcgta atatatgtgt actttgcagt tatgacgcca gatggcagta gtggaagata
     6601 ttctttattg aaaaatagct tgtcacctta cgtacaatct tgatccggag cttttctttt
     6661 tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc
     6721 attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta
     6781 tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga
     6841 gcacagaggc cgcagaatgt gctctagatt ccgatgctga cttgctgggt attatatgtg
     6901 tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca agtcttgtaa
     6961 aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt cgtaatcaac
     7021 ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc caactgcatg
     7081 gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt aaaagactcg
     7141 tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct cattcgttta
     7201 ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg aactcgattt
     7261 ctgactgggt tggaaggcaa gagagccccg aaagcttaca ttttatgtta gctggtggac
     7321 tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt gttgatgtaa
     7381 gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa
     7441 atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg tgcacttgcc
     7501 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa
     7561 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     7621 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     7681 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     7741 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     7801 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     7861 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     7921 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7981 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa
     8041 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     8101 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     8161 agtgaattgt aatacgactc actatagggc gaattgggat ccgtgtggaa gaacgattac
     8221 aacaggtatt gtcctctgag gacataaaat acacaccgag attcatcaac tcattgctgg
     8281 agttagcata tctacaattg ggtgaaatgg ggagcgattt gcaggcattt gctcggcatg
     8341 ccggtagagg tgtggtcaat aagagcgacc tcatgctata cctgagaaag caacctgacc
     8401 tacaggaaag agttactcaa gaacaagaat tttcgtttta aaacctaaga gtcactttaa
     8461 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
     8521 aaattcgctt atttagaagt gtcaacaacg tatctaccaa cgatttgacc cttttccatc
     8581 ttttcgtaaa tttctggcaa ggtagacaag ccgacaacct tgattggaga cttgaccaaa
     8641 cctctggcga agaagtccaa agctccaccg cggtggcggc cgttacttac ttagagctcg
     8701 acgtcttact tacttagcgg ccgcactagt agatccttgc gtgacaattc ttgcgtgaca
     8761 attcttgcgt gacaattctt gcgtgagaat tcttgcgtga caatgtcgac ccggg
//
