LOCUS       pEHC                    8129 bp    DNA     circular     05-JUN-2018
FEATURES             Location/Qualifiers
     misc_feature    1195..2217
                     /note="HygromycinPhosphotransferase"
     misc_feature    2287..4269
                     /note="CreERT2"
     misc_feature    2221..2286
                     /note="P2A Codon V2"
     misc_feature    4280..4814
                     /note="Rabbit globin polyA"
     misc_feature    4937..8046
                     /note="Vector Backbone from pd2EGFP-1 (clontech) 
                     contains f1ori, Sv40Promoter driving Kanr/Neor cassette
                     and pUC ori"
     misc_feature    
                     /note="Cv1R Primer"
     misc_feature    28..405
                     /note="H2B  "
     misc_feature    406..411
                     /note="link"
     misc_feature    1129..1194
                     /note="P2A Codon Version 1  (JH Kim et al 2011)"
                     /note=""
     misc_feature    412..1128
                     /note="EmGFP"
     source          1..8129
                     /dnas_title="pEHC"
ORIGIN      
        1 GGCAAAGAAT TCATGAGGCC GGCCACCATG ccagagccag cgaagtctgc tcccgccccg
       61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag
      121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
      181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
      241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
      301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
      361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac cATGGTGAGC
      421 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
      481 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
      541 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
      601 ACCTTGACCT ACGGCGTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
      661 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
      721 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
      781 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
      841 TACAACTACA ACAGCCACAA GGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG
      901 GTGAACTTCA AGACCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
      961 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
     1021 ACCCAGTCCA AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
     1081 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGGG AAGCGGAGCT
     1141 ACTAACTTCA GCCTGCTGAA GCAGGCTGGA GACGTGGAGG AGAACCCTGG ACCTatgaaa
     1201 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     1261 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     1321 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     1381 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     1441 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     1501 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     1561 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     1621 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     1681 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     1741 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     1801 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     1861 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     1921 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     1981 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     2041 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     2101 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     2161 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaacgc
     2221 GGCTCCGGCG CAACAAATTT CTCTCTCCTC AAACAAGCAG GCGATGTTGA AGAAAATCCC
     2281 GGGCCCATGT CCAATTTACT GACCGTACAC CAAAATTTGC CTGCATTACC GGTCGATGCA
     2341 ACGAGTGATG AGGTTCGCAA GAACCTGATG GACATGTTCA GGGATCGCCA GGCGTTTTCT
     2401 GAGCATACCT GGAAAATGCT TCTGTCCGTT TGCCGGTCGT GGGCGGCATG GTGCAAGTTG
     2461 AATAACCGGA AATGGTTTCC CGCAGAACCT GAAGATGTTC GCGATTATCT TCTATATCTT
     2521 CAGGCGCGCG GTCTGGCAGT AAAAACTATC CAGCAACATT TGGGCCAGCT AAACATGCTT
     2581 CATCGTCGGT CCGGGCTGCC ACGACCAAGT GACAGCAATG CTGTTTCACT GGTTATGCGG
     2641 CGGATCCGAA AAGAAAACGT TGATGCCGGT GAACGTGCAA AACAGGCTCT AGCGTTCGAA
     2701 CGCACTGATT TCGACCAGGT TCGTTCACTC ATGGAAAATA GCGATCGCTG CCAGGATATA
     2761 CGTAATCTGG CATTTCTGGG GATTGCTTAT AACACCCTGT TACGTATAGC CGAAATTGCC
     2821 AGGATCAGGG TTAAAGATAT CTCACGTACT GACGGTGGGA GAATGTTAAT CCATATTGGC
     2881 AGAACGAAAA CGCTGGTTAG CACCGCAGGT GTAGAGAAGG CACTTAGCCT GGGGGTAACT
     2941 AAACTGGTCG AGCGATGGAT TTCCGTCTCT GGTGTAGCTG ATGATCCGAA TAACTACCTG
     3001 TTTTGCCGGG TCAGAAAAAA TGGTGTTGCC GCGCCATCTG CCACCAGCCA GCTATCAACT
     3061 CGCGCCCTGG AAGGGATTTT TGAAGCAACT CATCGATTGA TTTACGGCGC TAAGGATGAC
     3121 TCTGGTCAGA GATACCTGGC CTGGTCTGGA CACAGTGCCC GTGTCGGAGC CGCGCGAGAT
     3181 ATGGCCCGCG CTGGAGTTTC AATACCGGAG ATCATGCAAG CTGGTGGCTG GACCAATGTA
     3241 AATATTGTCA TGAACTATAT CCGTAACCTG GATAGTGAAA CAGGGGCAAT GGTGCGCCTG
     3301 CTGGAAGATG GCGATCTCGA GCCATCTGCT GGAGACATGA GAGCTGCCAA CCTTTGGCCA
     3361 AGCCCGCTCA TGATCAAACG CTCTAAGAAG AACAGCCTGG CCTTGTCCCT GACGGCCGAC
     3421 CAGATGGTCA GTGCCTTGTT GGATGCTGAG CCCCCCATAC TCTATTCCGA GTATGATCCT
     3481 ACCAGACCCT TCAGTGAAGC TTCGATGATG GGCTTACTGA CCAACCTGGC AGACAGGGAG
     3541 CTGGTTCACA TGATCAACTG GGCGAAGAGG GTGCCAGGCT TTGTGGATTT GACCCTCCAT
     3601 GATCAGGTCC ACCTTCTAGA ATGTGCCTGG CTAGAGATCC TGATGATTGG TCTCGTCTGG
     3661 CGCTCCATGG AGCACCCAGT GAAGCTACTG TTTGCTCCTA ACTTGCTCTT GGACAGGAAC
     3721 CAGGGAAAAT GTGTAGAGGG CATGGTGGAG ATCTTCGACA TGCTGCTGGC TACATCATCT
     3781 CGGTTCCGCA TGATGAATCT GCAGGGAGAG GAGTTTGTGT GCCTCAAATC TATTATTTTG
     3841 CTTAATTCTG GAGTGTACAC ATTTCTGTCC AGCACCCTGA AGTCTCTGGA AGAGAAGGAC
     3901 CATATCCACC GAGTCCTGGA CAAGATCACA GACACTTTGA TCCACCTGAT GGCCAAGGCA
     3961 GGCCTGACCC TGCAGCAGCA GCACCAGCGG CTGGCCCAGC TCCTCCTCAT CCTCTCCCAC
     4021 ATCAGGCACA TGAGTAACAA AGGCATGGAG CATCTGTACA GCATGAAGTG CAAGAACGTG
     4081 GTGCCCCTCT ATGACCTGCT GCTGGAGGCG GCGGACGCCC ACCGCCTACA TGCGCCCACT
     4141 AGCCGTGGAG GGGCATCCGT GGAGGAGACG GACCAAAGCC ACTTGGCCAC TGCGGGCTCT
     4201 ACTTCATCGC ATTCCTTGCA AAAGTATTAC ATCACGGGGG AGGCAGAGGG TTTCCCTGCC
     4261 ACAGCTTGAT AGCGGCCGCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG
     4321 TGTGGCCAAT GCCCTGGCTC ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA
     4381 TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT
     4441 CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC
     4501 AAATCATTTA AAACATCAGA ATGAGTATTT GGTTTAGAGT TTGGCAACAT ATGCCATATG
     4561 CTGGCTGCCA TGAACAAAGG TGGCTATAAA GAGGTCATCA GTATATGAAA CAGCCCCCTG
     4621 CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT TTTATATTTT
     4681 GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGTT TTACTAGCCA
     4741 GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT CCCTCTTCTC TTATGAAGAT
     4801 CCCTCGACCT GCAGCCCAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
     4861 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAT GCATAAAGTG TAAAGCCTGG
     4921 GGTGCCTAAT GAGTGAAACT CATCAATGTA TCTTAAGGCG TAAATTGTAA GCGTTAATAT
     4981 TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA
     5041 AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC
     5101 AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC
     5161 CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC
     5221 GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG
     5281 GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG
     5341 GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC
     5401 GCCGCTACAG GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
     5461 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
     5521 GCTTCAATAA TATTGAAAAA GGAAGAGTCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG
     5581 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG
     5641 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
     5701 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC
     5761 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT
     5821 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC
     5881 TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG
     5941 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
     6001 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
     6061 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
     6121 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
     6181 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
     6241 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
     6301 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
     6361 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
     6421 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
     6481 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
     6541 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA
     6601 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
     6661 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT
     6721 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC
     6781 CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC
     6841 GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC
     6901 GCCCACCCTA GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC
     6961 GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA
     7021 CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC
     7081 CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA
     7141 GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CCTCAGGTTA CTCATATATA
     7201 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT
     7261 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC
     7321 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG
     7381 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT
     7441 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG
     7501 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG
     7561 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC
     7621 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA
     7681 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA
     7741 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC
     7801 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT
     7861 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG
     7921 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT
     7981 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC
     8041 ATGCATTAGC GCTACCGGAC TCAGATCTCG AGCTCAAGCT TCGAATTCTG CAGTCGACGG
     8101 TACCGCGGGC CCGGGATCCA CCGGTCGCC
//

