LOCUS       pmTHC                   8120 bp    DNA     circular     05-JUN-2018
FEATURES             Location/Qualifiers
     misc_feature    1186..2208
                     /note="HygromycinPhosphotransferase"
     misc_feature    2278..4260
                     /note="CreERT2"
     misc_feature    4271..4805
                     /note="Rabbit globin polyA"
     misc_feature    4928..8037
                     /note="Vector Backbone from pd2EGFP-1 (clontech) 
                     contains f1ori, Sv40Promoter driving Kanr/Neor cassette
                     and pUC ori"
     misc_feature    
                     /note="Cv1R Primer"
     misc_feature    28..405
                     /note="H2B  "
     misc_feature    406..411
                     /note="link"
     misc_feature    1120..1185
                     /note="P2A Codon Version 1  (JH Kim et al 2011)"
                     /note=""
     misc_feature    412..1119
                     /note="mTFP1"
     source          1..8120
                     /dnas_title="pmTHC"
ORIGIN      
        1 GGCAAAGAAT TCATGAGGCC GGCCACCATG ccagagccag cgaagtctgc tcccgccccg
       61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag
      121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
      181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
      241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
      301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
      361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac cATGGTGAGC
      421 AAGGGCGAGG AGACCACAAT GGGCGTAATC AAGCCCGACA TGAAGATCAA GCTGAAGATG
      481 GAGGGCAACG TGAATGGCCA CGCCTTCGTG ATCGAGGGCG AGGGCGAGGG CAAGCCCTAC
      541 GACGGCACCA ACACCATCAA CCTGGAGGTG AAGGAGGGAG CCCCCCTGCC CTTCTCCTAC
      601 GACATTCTGA CCACCGCGTT CGCCTACGGC AACAGGGCCT TCACCAAGTA CCCCGACGAC
      661 ATCCCCAACT ACTTCAAGCA GTCCTTCCCC GAGGGCTACT CTTGGGAGCG CACCATGACC
      721 TTCGAGGACA AGGGCATCGT GAAGGTGAAG TCCGACATCT CCATGGAGGA GGACTCCTTC
      781 ATCTACGAGA TACACCTCAA GGGCGAGAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG
      841 AAGACCACCG GCTGGGACGC CTCCACCGAG AGGATGTACG TGCGCGACGG CGTGCTGAAG
      901 GGCGACGTCA AGCACAAGCT GCTGCTGGAG GGCGGCGGCC ACCACCGCGT TGACTTCAAG
      961 ACCATCTACA GGGCCAAGAA GGCGGTGAAG CTGCCCGACT ATCACTTTGT GGACCACCGC
     1021 ATCGAGATCC TGAACCACGA CAAGGACTAC AACAAGGTGA CCGTTTACGA GAGCGCCGTG
     1081 GCCCGCAACT CCACCGACGG CATGGACGAG CTGTACAAGG GAAGCGGAGC TACTAACTTC
     1141 AGCCTGCTGA AGCAGGCTGG AGACGTGGAG GAGAACCCTG GACCTatgaa aaagcctgaa
     1201 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     1261 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     1321 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     1381 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     1441 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     1501 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     1561 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     1621 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     1681 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     1741 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     1801 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     1861 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     1921 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     1981 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2041 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2101 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2161 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaacg cGGCTCCGGC
     2221 GCAACAAATT TCTCTCTCCT CAAACAAGCA GGCGATGTTG AAGAAAATCC CGGGCCCATG
     2281 TCCAATTTAC TGACCGTACA CCAAAATTTG CCTGCATTAC CGGTCGATGC AACGAGTGAT
     2341 GAGGTTCGCA AGAACCTGAT GGACATGTTC AGGGATCGCC AGGCGTTTTC TGAGCATACC
     2401 TGGAAAATGC TTCTGTCCGT TTGCCGGTCG TGGGCGGCAT GGTGCAAGTT GAATAACCGG
     2461 AAATGGTTTC CCGCAGAACC TGAAGATGTT CGCGATTATC TTCTATATCT TCAGGCGCGC
     2521 GGTCTGGCAG TAAAAACTAT CCAGCAACAT TTGGGCCAGC TAAACATGCT TCATCGTCGG
     2581 TCCGGGCTGC CACGACCAAG TGACAGCAAT GCTGTTTCAC TGGTTATGCG GCGGATCCGA
     2641 AAAGAAAACG TTGATGCCGG TGAACGTGCA AAACAGGCTC TAGCGTTCGA ACGCACTGAT
     2701 TTCGACCAGG TTCGTTCACT CATGGAAAAT AGCGATCGCT GCCAGGATAT ACGTAATCTG
     2761 GCATTTCTGG GGATTGCTTA TAACACCCTG TTACGTATAG CCGAAATTGC CAGGATCAGG
     2821 GTTAAAGATA TCTCACGTAC TGACGGTGGG AGAATGTTAA TCCATATTGG CAGAACGAAA
     2881 ACGCTGGTTA GCACCGCAGG TGTAGAGAAG GCACTTAGCC TGGGGGTAAC TAAACTGGTC
     2941 GAGCGATGGA TTTCCGTCTC TGGTGTAGCT GATGATCCGA ATAACTACCT GTTTTGCCGG
     3001 GTCAGAAAAA ATGGTGTTGC CGCGCCATCT GCCACCAGCC AGCTATCAAC TCGCGCCCTG
     3061 GAAGGGATTT TTGAAGCAAC TCATCGATTG ATTTACGGCG CTAAGGATGA CTCTGGTCAG
     3121 AGATACCTGG CCTGGTCTGG ACACAGTGCC CGTGTCGGAG CCGCGCGAGA TATGGCCCGC
     3181 GCTGGAGTTT CAATACCGGA GATCATGCAA GCTGGTGGCT GGACCAATGT AAATATTGTC
     3241 ATGAACTATA TCCGTAACCT GGATAGTGAA ACAGGGGCAA TGGTGCGCCT GCTGGAAGAT
     3301 GGCGATCTCG AGCCATCTGC TGGAGACATG AGAGCTGCCA ACCTTTGGCC AAGCCCGCTC
     3361 ATGATCAAAC GCTCTAAGAA GAACAGCCTG GCCTTGTCCC TGACGGCCGA CCAGATGGTC
     3421 AGTGCCTTGT TGGATGCTGA GCCCCCCATA CTCTATTCCG AGTATGATCC TACCAGACCC
     3481 TTCAGTGAAG CTTCGATGAT GGGCTTACTG ACCAACCTGG CAGACAGGGA GCTGGTTCAC
     3541 ATGATCAACT GGGCGAAGAG GGTGCCAGGC TTTGTGGATT TGACCCTCCA TGATCAGGTC
     3601 CACCTTCTAG AATGTGCCTG GCTAGAGATC CTGATGATTG GTCTCGTCTG GCGCTCCATG
     3661 GAGCACCCAG TGAAGCTACT GTTTGCTCCT AACTTGCTCT TGGACAGGAA CCAGGGAAAA
     3721 TGTGTAGAGG GCATGGTGGA GATCTTCGAC ATGCTGCTGG CTACATCATC TCGGTTCCGC
     3781 ATGATGAATC TGCAGGGAGA GGAGTTTGTG TGCCTCAAAT CTATTATTTT GCTTAATTCT
     3841 GGAGTGTACA CATTTCTGTC CAGCACCCTG AAGTCTCTGG AAGAGAAGGA CCATATCCAC
     3901 CGAGTCCTGG ACAAGATCAC AGACACTTTG ATCCACCTGA TGGCCAAGGC AGGCCTGACC
     3961 CTGCAGCAGC AGCACCAGCG GCTGGCCCAG CTCCTCCTCA TCCTCTCCCA CATCAGGCAC
     4021 ATGAGTAACA AAGGCATGGA GCATCTGTAC AGCATGAAGT GCAAGAACGT GGTGCCCCTC
     4081 TATGACCTGC TGCTGGAGGC GGCGGACGCC CACCGCCTAC ATGCGCCCAC TAGCCGTGGA
     4141 GGGGCATCCG TGGAGGAGAC GGACCAAAGC CACTTGGCCA CTGCGGGCTC TACTTCATCG
     4201 CATTCCTTGC AAAAGTATTA CATCACGGGG GAGGCAGAGG GTTTCCCTGC CACAGCTTGA
     4261 TAGCGGCCGC ACTCCTCAGG TGCAGGCTGC CTATCAGAAG GTGGTGGCTG GTGTGGCCAA
     4321 TGCCCTGGCT CACAAATACC ACTGAGATCT TTTTCCCTCT GCCAAAAATT ATGGGGACAT
     4381 CATGAAGCCC CTTGAGCATC TGACTTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT
     4441 AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT
     4501 AAAACATCAG AATGAGTATT TGGTTTAGAG TTTGGCAACA TATGCCATAT GCTGGCTGCC
     4561 ATGAACAAAG GTGGCTATAA AGAGGTCATC AGTATATGAA ACAGCCCCCT GCTGTCCATT
     4621 CCTTATTCCA TAGAAAAGCC TTGACTTGAG GTTAGATTTT TTTTATATTT TGTTTTGTGT
     4681 TATTTTTTTC TTTAACATCC CTAAAATTTT CCTTACATGT TTTACTAGCC AGATTTTTCC
     4741 TCCTCTCCTG ACTACTCCCA GTCATAGCTG TCCCTCTTCT CTTATGAAGA TCCCTCGACC
     4801 TGCAGCCCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
     4861 CTCACAATTC CACACAACAT ACGAGCCGGA TGCATAAAGT GTAAAGCCTG GGGTGCCTAA
     4921 TGAGTGAAAC TCATCAATGT ATCTTAAGGC GTAAATTGTA AGCGTTAATA TTTTGTTAAA
     4981 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
     5041 AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
     5101 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
     5161 GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG
     5221 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
     5281 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
     5341 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
     5401 GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
     5461 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
     5521 ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA
     5581 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
     5641 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC
     5701 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
     5761 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA
     5821 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
     5881 GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA
     5941 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA
     6001 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG
     6061 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA
     6121 GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT
     6181 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT
     6241 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT
     6301 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG
     6361 AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA
     6421 GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA
     6481 TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT
     6541 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT
     6601 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT
     6661 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT
     6721 CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA
     6781 CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG
     6841 GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCT
     6901 AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG
     6961 GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT
     7021 CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC
     7081 CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA
     7141 GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCTCAGGTT ACTCATATAT ACTTTAGATT
     7201 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
     7261 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     7321 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     7381 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     7441 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     7501 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     7561 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     7621 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     7681 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     7741 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     7801 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     7861 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     7921 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     7981 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CATGCATTAG
     8041 CGCTACCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG
     8101 CCCGGGATCC ACCGGTCGCC
//

