LOCUS       ptdTHC                  8840 bp    DNA     circular     05-JUN-2018
FEATURES             Location/Qualifiers
     misc_feature    1906..2928
                     /note="HygromycinPhosphotransferase"
     misc_feature    2998..4980
                     /note="CreERT2"
     misc_feature    2932..2997
                     /note="P2A Codon V2"
     misc_feature    4991..5525
                     /note="Rabbit globin polyA"
     misc_feature    5648..8757
                     /note="Vector Backbone from pd2EGFP-1 (clontech) 
                     contains f1ori, Sv40Promoter driving Kanr/Neor cassette
                     and pUC ori"
     misc_feature    
                     /note="Cv1R Primer"
     misc_feature    28..405
                     /note="H2B  "
     misc_feature    406..411
                     /note="link"
     misc_feature    1840..1905
                     /note="P2A Codon Version 1  (JH Kim et al 2011)"
                     /note=""
     misc_feature    412..1839
                     /note=" tdTomato ORF"
     source          1..8840
                     /dnas_title="ptdTHC"
ORIGIN      
        1 GGCAAAGAAT TCATGAGGCC GGCCACCATG ccagagccag cgaagtctgc tcccgccccg
       61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag
      121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
      181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
      241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
      301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
      361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac cATGGTGAGC
      421 AAGGGAGAGG AGGTCATCAA AGAGTTCATG CGCTTCAAGG TGCGCATGGA GGGCTCCATG
      481 AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG
      541 ACCGCCAAGC TGAAGGTGAC CAAGGGCGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC
      601 CCCCAGTTCA TGTACGGCTC CAAGGCGTAC GTGAAGCACC CCGCCGACAT CCCCGATTAC
      661 AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC
      721 GGTCTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCACGCTGAT CTACAAGGTG
      781 AAGATGCGCG GCACCAACTT CCCCCCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC
      841 TGGGAGGCCT CCACCGAGCG CCTGTACCCC CGCGACGGCG TGCTGAAGGG CGAGATCCAC
      901 CAGGCCCTGA AGCTGAAGGA CGGCGGCCAC TACCTGGTGG AGTTCAAGAC CATCTACATG
      961 GCCAAGAAGC CCGTGCAACT GCCCGGCTAC TACTACGTGG ACACCAAGCT GGACATCACC
     1021 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAGC GCTCCGAGGG CCGCCACCAC
     1081 CTGTTCCTGG GGCATGGCAC CGGCAGCACC GGCAGCGGCA GCTCCGGCAC CGCCTCCTCC
     1141 GAGGACAACA ACATGGCCGT CATCAAAGAG TTCATGCGCT TCAAGGTGCG CATGGAGGGC
     1201 TCCATGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
     1261 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGCGGCCCCC TGCCCTTCGC CTGGGACATC
     1321 CTGTCCCCCC AGTTCATGTA CGGCTCCAAG GCGTACGTGA AGCACCCCGC CGACATCCCC
     1381 GATTACAAGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG
     1441 GACGGCGGTC TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCAC GCTGATCTAC
     1501 AAGGTGAAGA TGCGCGGCAC CAACTTCCCC CCCGACGGCC CCGTAATGCA GAAGAAGACC
     1561 ATGGGCTGGG AGGCCTCCAC CGAGCGCCTG TACCCCCGCG ACGGCGTGCT GAAGGGCGAG
     1621 ATCCACCAGG CCCTGAAGCT GAAGGACGGC GGCCGCTACC TGGTGGAGTT CAAGACCATC
     1681 TACATGGCCA AGAAGCCCGT GCAACTGCCC GGCTACTACT ACGTGGACAC CAAGCTGGAC
     1741 ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAGCGCTC CGAGGGCCGC
     1801 CACCACCTGT TCCTGTACGG CATGGACGAG CTGTACAAGG GAAGCGGAGC TACTAACTTC
     1861 AGCCTGCTGA AGCAGGCTGG AGACGTGGAG GAGAACCCTG GACCTatgaa aaagcctgaa
     1921 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     1981 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     2041 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     2101 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     2161 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     2221 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     2281 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     2341 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     2401 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     2461 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     2521 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     2581 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     2641 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     2701 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2761 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2821 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2881 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaacg cGGCTCCGGC
     2941 GCAACAAATT TCTCTCTCCT CAAACAAGCA GGCGATGTTG AAGAAAATCC CGGGCCCATG
     3001 TCCAATTTAC TGACCGTACA CCAAAATTTG CCTGCATTAC CGGTCGATGC AACGAGTGAT
     3061 GAGGTTCGCA AGAACCTGAT GGACATGTTC AGGGATCGCC AGGCGTTTTC TGAGCATACC
     3121 TGGAAAATGC TTCTGTCCGT TTGCCGGTCG TGGGCGGCAT GGTGCAAGTT GAATAACCGG
     3181 AAATGGTTTC CCGCAGAACC TGAAGATGTT CGCGATTATC TTCTATATCT TCAGGCGCGC
     3241 GGTCTGGCAG TAAAAACTAT CCAGCAACAT TTGGGCCAGC TAAACATGCT TCATCGTCGG
     3301 TCCGGGCTGC CACGACCAAG TGACAGCAAT GCTGTTTCAC TGGTTATGCG GCGGATCCGA
     3361 AAAGAAAACG TTGATGCCGG TGAACGTGCA AAACAGGCTC TAGCGTTCGA ACGCACTGAT
     3421 TTCGACCAGG TTCGTTCACT CATGGAAAAT AGCGATCGCT GCCAGGATAT ACGTAATCTG
     3481 GCATTTCTGG GGATTGCTTA TAACACCCTG TTACGTATAG CCGAAATTGC CAGGATCAGG
     3541 GTTAAAGATA TCTCACGTAC TGACGGTGGG AGAATGTTAA TCCATATTGG CAGAACGAAA
     3601 ACGCTGGTTA GCACCGCAGG TGTAGAGAAG GCACTTAGCC TGGGGGTAAC TAAACTGGTC
     3661 GAGCGATGGA TTTCCGTCTC TGGTGTAGCT GATGATCCGA ATAACTACCT GTTTTGCCGG
     3721 GTCAGAAAAA ATGGTGTTGC CGCGCCATCT GCCACCAGCC AGCTATCAAC TCGCGCCCTG
     3781 GAAGGGATTT TTGAAGCAAC TCATCGATTG ATTTACGGCG CTAAGGATGA CTCTGGTCAG
     3841 AGATACCTGG CCTGGTCTGG ACACAGTGCC CGTGTCGGAG CCGCGCGAGA TATGGCCCGC
     3901 GCTGGAGTTT CAATACCGGA GATCATGCAA GCTGGTGGCT GGACCAATGT AAATATTGTC
     3961 ATGAACTATA TCCGTAACCT GGATAGTGAA ACAGGGGCAA TGGTGCGCCT GCTGGAAGAT
     4021 GGCGATCTCG AGCCATCTGC TGGAGACATG AGAGCTGCCA ACCTTTGGCC AAGCCCGCTC
     4081 ATGATCAAAC GCTCTAAGAA GAACAGCCTG GCCTTGTCCC TGACGGCCGA CCAGATGGTC
     4141 AGTGCCTTGT TGGATGCTGA GCCCCCCATA CTCTATTCCG AGTATGATCC TACCAGACCC
     4201 TTCAGTGAAG CTTCGATGAT GGGCTTACTG ACCAACCTGG CAGACAGGGA GCTGGTTCAC
     4261 ATGATCAACT GGGCGAAGAG GGTGCCAGGC TTTGTGGATT TGACCCTCCA TGATCAGGTC
     4321 CACCTTCTAG AATGTGCCTG GCTAGAGATC CTGATGATTG GTCTCGTCTG GCGCTCCATG
     4381 GAGCACCCAG TGAAGCTACT GTTTGCTCCT AACTTGCTCT TGGACAGGAA CCAGGGAAAA
     4441 TGTGTAGAGG GCATGGTGGA GATCTTCGAC ATGCTGCTGG CTACATCATC TCGGTTCCGC
     4501 ATGATGAATC TGCAGGGAGA GGAGTTTGTG TGCCTCAAAT CTATTATTTT GCTTAATTCT
     4561 GGAGTGTACA CATTTCTGTC CAGCACCCTG AAGTCTCTGG AAGAGAAGGA CCATATCCAC
     4621 CGAGTCCTGG ACAAGATCAC AGACACTTTG ATCCACCTGA TGGCCAAGGC AGGCCTGACC
     4681 CTGCAGCAGC AGCACCAGCG GCTGGCCCAG CTCCTCCTCA TCCTCTCCCA CATCAGGCAC
     4741 ATGAGTAACA AAGGCATGGA GCATCTGTAC AGCATGAAGT GCAAGAACGT GGTGCCCCTC
     4801 TATGACCTGC TGCTGGAGGC GGCGGACGCC CACCGCCTAC ATGCGCCCAC TAGCCGTGGA
     4861 GGGGCATCCG TGGAGGAGAC GGACCAAAGC CACTTGGCCA CTGCGGGCTC TACTTCATCG
     4921 CATTCCTTGC AAAAGTATTA CATCACGGGG GAGGCAGAGG GTTTCCCTGC CACAGCTTGA
     4981 TAGCGGCCGC ACTCCTCAGG TGCAGGCTGC CTATCAGAAG GTGGTGGCTG GTGTGGCCAA
     5041 TGCCCTGGCT CACAAATACC ACTGAGATCT TTTTCCCTCT GCCAAAAATT ATGGGGACAT
     5101 CATGAAGCCC CTTGAGCATC TGACTTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT
     5161 AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT
     5221 AAAACATCAG AATGAGTATT TGGTTTAGAG TTTGGCAACA TATGCCATAT GCTGGCTGCC
     5281 ATGAACAAAG GTGGCTATAA AGAGGTCATC AGTATATGAA ACAGCCCCCT GCTGTCCATT
     5341 CCTTATTCCA TAGAAAAGCC TTGACTTGAG GTTAGATTTT TTTTATATTT TGTTTTGTGT
     5401 TATTTTTTTC TTTAACATCC CTAAAATTTT CCTTACATGT TTTACTAGCC AGATTTTTCC
     5461 TCCTCTCCTG ACTACTCCCA GTCATAGCTG TCCCTCTTCT CTTATGAAGA TCCCTCGACC
     5521 TGCAGCCCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
     5581 CTCACAATTC CACACAACAT ACGAGCCGGA TGCATAAAGT GTAAAGCCTG GGGTGCCTAA
     5641 TGAGTGAAAC TCATCAATGT ATCTTAAGGC GTAAATTGTA AGCGTTAATA TTTTGTTAAA
     5701 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
     5761 AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
     5821 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
     5881 GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG
     5941 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
     6001 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
     6061 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
     6121 GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
     6181 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
     6241 ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA
     6301 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
     6361 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC
     6421 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
     6481 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA
     6541 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
     6601 GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA
     6661 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA
     6721 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG
     6781 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA
     6841 GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT
     6901 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT
     6961 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT
     7021 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG
     7081 AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA
     7141 GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA
     7201 TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT
     7261 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT
     7321 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT
     7381 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT
     7441 CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA
     7501 CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG
     7561 GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCT
     7621 AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG
     7681 GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT
     7741 CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC
     7801 CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA
     7861 GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCTCAGGTT ACTCATATAT ACTTTAGATT
     7921 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
     7981 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     8041 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     8101 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     8161 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     8221 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     8281 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     8341 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     8401 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     8461 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     8521 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     8581 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     8641 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     8701 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CATGCATTAG
     8761 CGCTACCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG
     8821 CCCGGGATCC ACCGGTCGCC
//

