LOCUS pCAG-mTHC 9843 bp DNA circular 05-JUN-2018 FEATURES Location/Qualifiers misc_feature 1186..2208 /note="HygromycinPhosphotransferase" misc_feature 2278..4260 /note="CreERT2" misc_feature 2212..2277 /note="P2A Codon V2" misc_feature 4271..4807 /note="Rabbit globin polyA" misc_feature 4930..8039 /note="Vector Backbone from pd2EGFP-1 (clontech) contains f1ori, Sv40Promoter driving Kanr/Neor cassette and pUC ori" misc_feature /note="Cv1R Primer" misc_feature 28..405 /note="H2B " misc_feature 406..411 /note="link" misc_feature 1120..1185 /note="P2A Codon Version 1 (JH Kim et al 2011)" /note="" misc_feature 8096..9843 /note="CAG Promoter" misc_feature 412..1119 /note="mTFP1" source 1..9843 /dnas_title="pCAG-mTHC" ORIGIN 1 GGCAAAGAAT TCATGAGGCC GGCCACCATG ccagagccag cgaagtctgc tcccgccccg 61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag 121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct 181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc 241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc 301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc 361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac cATGGTGAGC 421 AAGGGCGAGG AGACCACAAT GGGCGTAATC AAGCCCGACA TGAAGATCAA GCTGAAGATG 481 GAGGGCAACG TGAATGGCCA CGCCTTCGTG ATCGAGGGCG AGGGCGAGGG CAAGCCCTAC 541 GACGGCACCA ACACCATCAA CCTGGAGGTG AAGGAGGGAG CCCCCCTGCC CTTCTCCTAC 601 GACATTCTGA CCACCGCGTT CGCCTACGGC AACAGGGCCT TCACCAAGTA CCCCGACGAC 661 ATCCCCAACT ACTTCAAGCA GTCCTTCCCC GAGGGCTACT CTTGGGAGCG CACCATGACC 721 TTCGAGGACA AGGGCATCGT GAAGGTGAAG TCCGACATCT CCATGGAGGA GGACTCCTTC 781 ATCTACGAGA TACACCTCAA GGGCGAGAAC TTCCCCCCCA ACGGCCCCGT GATGCAGAAG 841 AAGACCACCG GCTGGGACGC CTCCACCGAG AGGATGTACG TGCGCGACGG CGTGCTGAAG 901 GGCGACGTCA AGCACAAGCT GCTGCTGGAG GGCGGCGGCC ACCACCGCGT TGACTTCAAG 961 ACCATCTACA GGGCCAAGAA GGCGGTGAAG CTGCCCGACT ATCACTTTGT GGACCACCGC 1021 ATCGAGATCC TGAACCACGA CAAGGACTAC AACAAGGTGA CCGTTTACGA GAGCGCCGTG 1081 GCCCGCAACT CCACCGACGG CATGGACGAG CTGTACAAGG GAAGCGGAGC TACTAACTTC 1141 AGCCTGCTGA AGCAGGCTGG AGACGTGGAG GAGAACCCTG GACCTatgaa aaagcctgaa 1201 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg 1261 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga 1321 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg 1381 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag 1441 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa 1501 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc 1561 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact 1621 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg 1681 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc 1741 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg 1801 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc 1861 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag 1921 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat 1981 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat 2041 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg 2101 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc 2161 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaacg cGGCTCCGGC 2221 GCAACAAATT TCTCTCTCCT CAAACAAGCA GGCGATGTTG AAGAAAATCC CGGGCCCATG 2281 TCCAATTTAC TGACCGTACA CCAAAATTTG CCTGCATTAC CGGTCGATGC AACGAGTGAT 2341 GAGGTTCGCA AGAACCTGAT GGACATGTTC AGGGATCGCC AGGCGTTTTC TGAGCATACC 2401 TGGAAAATGC TTCTGTCCGT TTGCCGGTCG TGGGCGGCAT GGTGCAAGTT GAATAACCGG 2461 AAATGGTTTC CCGCAGAACC TGAAGATGTT CGCGATTATC TTCTATATCT TCAGGCGCGC 2521 GGTCTGGCAG TAAAAACTAT CCAGCAACAT TTGGGCCAGC TAAACATGCT TCATCGTCGG 2581 TCCGGGCTGC CACGACCAAG TGACAGCAAT GCTGTTTCAC TGGTTATGCG GCGGATCCGA 2641 AAAGAAAACG TTGATGCCGG TGAACGTGCA AAACAGGCTC TAGCGTTCGA ACGCACTGAT 2701 TTCGACCAGG TTCGTTCACT CATGGAAAAT AGCGATCGCT GCCAGGATAT ACGTAATCTG 2761 GCATTTCTGG GGATTGCTTA TAACACCCTG TTACGTATAG CCGAAATTGC CAGGATCAGG 2821 GTTAAAGATA TCTCACGTAC TGACGGTGGG AGAATGTTAA TCCATATTGG CAGAACGAAA 2881 ACGCTGGTTA GCACCGCAGG TGTAGAGAAG GCACTTAGCC TGGGGGTAAC TAAACTGGTC 2941 GAGCGATGGA TTTCCGTCTC TGGTGTAGCT GATGATCCGA ATAACTACCT GTTTTGCCGG 3001 GTCAGAAAAA ATGGTGTTGC CGCGCCATCT GCCACCAGCC AGCTATCAAC TCGCGCCCTG 3061 GAAGGGATTT TTGAAGCAAC TCATCGATTG ATTTACGGCG CTAAGGATGA CTCTGGTCAG 3121 AGATACCTGG CCTGGTCTGG ACACAGTGCC CGTGTCGGAG CCGCGCGAGA TATGGCCCGC 3181 GCTGGAGTTT CAATACCGGA GATCATGCAA GCTGGTGGCT GGACCAATGT AAATATTGTC 3241 ATGAACTATA TCCGTAACCT GGATAGTGAA ACAGGGGCAA TGGTGCGCCT GCTGGAAGAT 3301 GGCGATCTCG AGCCATCTGC TGGAGACATG AGAGCTGCCA ACCTTTGGCC AAGCCCGCTC 3361 ATGATCAAAC GCTCTAAGAA GAACAGCCTG GCCTTGTCCC TGACGGCCGA CCAGATGGTC 3421 AGTGCCTTGT TGGATGCTGA GCCCCCCATA CTCTATTCCG AGTATGATCC TACCAGACCC 3481 TTCAGTGAAG CTTCGATGAT GGGCTTACTG ACCAACCTGG CAGACAGGGA GCTGGTTCAC 3541 ATGATCAACT GGGCGAAGAG GGTGCCAGGC TTTGTGGATT TGACCCTCCA TGATCAGGTC 3601 CACCTTCTAG AATGTGCCTG GCTAGAGATC CTGATGATTG GTCTCGTCTG GCGCTCCATG 3661 GAGCACCCAG TGAAGCTACT GTTTGCTCCT AACTTGCTCT TGGACAGGAA CCAGGGAAAA 3721 TGTGTAGAGG GCATGGTGGA GATCTTCGAC ATGCTGCTGG CTACATCATC TCGGTTCCGC 3781 ATGATGAATC TGCAGGGAGA GGAGTTTGTG TGCCTCAAAT CTATTATTTT GCTTAATTCT 3841 GGAGTGTACA CATTTCTGTC CAGCACCCTG AAGTCTCTGG AAGAGAAGGA CCATATCCAC 3901 CGAGTCCTGG ACAAGATCAC AGACACTTTG ATCCACCTGA TGGCCAAGGC AGGCCTGACC 3961 CTGCAGCAGC AGCACCAGCG GCTGGCCCAG CTCCTCCTCA TCCTCTCCCA CATCAGGCAC 4021 ATGAGTAACA AAGGCATGGA GCATCTGTAC AGCATGAAGT GCAAGAACGT GGTGCCCCTC 4081 TATGACCTGC TGCTGGAGGC GGCGGACGCC CACCGCCTAC ATGCGCCCAC TAGCCGTGGA 4141 GGGGCATCCG TGGAGGAGAC GGACCAAAGC CACTTGGCCA CTGCGGGCTC TACTTCATCG 4201 CATTCCTTGC AAAAGTATTA CATCACGGGG GAGGCAGAGG GTTTCCCTGC CACAGCTTGA 4261 TAGCGGCCGC ACTCCTCAGG TGCAGGCTGC CTATCAGAAG GTGGTGGCTG GTGTGGCCAA 4321 TGCCCTGGCT CACAAATACC ACTGAGATCT TTTTCCCTCT GCCAAAAATT ATGGGGACAT 4381 CATGAAGCCC CTTGAGCATC TGACTTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT 4441 AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT 4501 AAAACATCAG AATGAGTATT TGGTTTAGAG TTTGGCAACA TATGCCCATA TGCTGGCTGC 4561 CATGAACAAA GGTTGGCTAT AAAGAGGTCA TCAGTATATG AAACAGCCCC CTGCTGTCCA 4621 TTCCTTATTC CATAGAAAAG CCTTGACTTG AGGTTAGATT TTTTTTATAT TTTGTTTTGT 4681 GTTATTTTTT TCTTTAACAT CCCTAAAATT TTCCTTACAT GTTTTACTAG CCAGATTTTT 4741 CCTCCTCTCC TGACTACTCC CAGTCATAGC TGTCCCTCTT CTCTTATGGA GATCCCTCGA 4801 CCTGCAGCCC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 4861 CGCTCACAAT TCCACACAAC ATACGAGCCG GATGCATAAA GTGTAAAGCC TGGGGTGCCT 4921 AATGAGTGAA ACTCATCAAT GTATCTTAAG GCGTAAATTG TAAGCGTTAA TATTTTGTTA 4981 AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC 5041 AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG 5101 AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT 5161 CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC 5221 CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG 5281 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG 5341 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA 5401 CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 5461 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA 5521 TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT 5581 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 5641 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 5701 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 5761 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 5821 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 5881 GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 5941 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 6001 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 6061 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC 6121 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 6181 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 6241 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 6301 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 6361 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 6421 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG 6481 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 6541 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG 6601 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 6661 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 6721 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 6781 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 6841 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 6901 CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA 6961 CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG 7021 TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG 7081 CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG 7141 CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT ATACTTTAGA 7201 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC 7261 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 7321 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 7381 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 7441 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT 7501 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 7561 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 7621 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 7681 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG 7741 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 7801 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 7861 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 7921 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 7981 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCATGCATT 8041 AGTTATTACT AGCGCTACCG GACTCAGATC TCGAGCTCAA GCTTCGAATT CTGCAGTCGA 8101 CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA 8161 TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC 8221 GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT 8281 TTCCATTGAC GTCAATGGGT GGACTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA 8341 GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG 8401 CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA 8461 GTCATCGCTA TTACCATGGG TCGAGGTGAG CCCCACGTTC TGCTTCACTC TCCCCATCTC 8521 CCCCCCCTCC CCACCCCCAA TTTTGTATTT ATTTATTTTT TAATTATTTT GTGCAGCGAT 8581 GGGGGCGGGG GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG 8641 GGGCGAGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT 8701 TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG 8761 TCGCTGCGTT GCCTTCGCCC CGTGCCCCGC TCCGCGCCGC CTCGCGCCGC CCGCCCCGGC 8821 TCTGACTGAC CGCGTTACTC CCACAGGTGA GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT 8881 GTAATTAGCG CTTGGTTTAA TGACGGCTCG TTTCTTTTCT GTGGCTGCGT GAAAGCCTTA 8941 AAGGGCTCCG GGAGGGCCCT TTGTGCGGGG GGGAGCGGCT CGGGGGGTGC GTGCGTGTGT 9001 GTGTGCGTGG GGAGCGCCGC GTGCGGCCCG CGCTGCCCGG CGGCTGTGAG CGCTGCGGGC 9061 GCGGCGCGGG GCTTTGTGCG CTCCGCGTGT GCGCGAGGGG AGCGCGGCCG GGGGCGGTGC 9121 CCCGCGGTGC GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG 9181 GGGTGAGCAG GGGGTGTGGG CGCGGCGGTC GGGCTGTAAC CCCCCCCTGC ACCCCCCTCC 9241 CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTGCGGGGC GTGGCGCGGG 9301 GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC 9361 TCGGGCCGGG GAGGGCTCGG GGGAGGGGCG CGGCGGCCCC GGAGCGCCGG CGGCTGTCGA 9421 GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC GCAGGGACTT 9481 CCTTTGTCCC AAATCTGGCG GAGCCGAAAT CTGGGAGGCG CCGCCGCACC CCCTCTAGCG 9541 GGCGCGGGCG AAGCGGTGCG GCGCCGGCAG GAAGGAAATG GGCGGGGAGG GCCTTCGTGC 9601 GTCGCCGCGC CGCCGTCCCC TTCTCCATCT CCAGCCTCGG GGCTGCCGCA GGGGGACGGC 9661 TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT 9721 AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG 9781 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGGATGA TCCACCGGTC 9841 GCC //