LOCUS Exported 8622 bp ds-DNA circular SYN 07-JUN-2018 DEFINITION pCMV-BE3. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 8622) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 7, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8622 /organism="unspecified" /mol_type="genomic DNA" source 404..5661 /organism="synthetic DNA construct" source 409..528 /organism="synthetic DNA construct" source 409..528 /organism="pCMV-BE3" source 1264..5364 /label=Source_1 misc_feature 128..403 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242743721.14 misc_feature 128..403 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496178408364.0 misc_feature 286..306 /label=CMV_fwd_primer promoter 287..356 /label=CMV_promoter promoter 377..395 /label=T7_promoter misc_feature 404..1233 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1510431651489.0 misc_feature 404..408 /label=KOZAK misc_feature 404..408 /label=Extracted region from pLenti_BE3RA-FNLS-PGK-Puro misc_feature 409..528 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177754792.0 misc_feature 409..528 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1502396267528.0 misc_feature 409..528 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1507847092960.0 misc_feature 409..528 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177991461.16 misc_feature 409..528 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177991440.15 misc_feature 409..528 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496178099654.28 misc_feature 409..528 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496178408364.0 misc_feature 409..528 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242795231.20 misc_feature 409..528 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1512593765171.8 misc_feature 409..528 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1512593831970.61 misc_feature 409..528 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1512594033117.62 misc_feature 409..528 /label=Extracted region from pCMV-FLAG-NLS-BE3* CDS 409..477 /label=3X FLAG CDS 478..528 /label=SV40 NLS misc_feature 529..1263 /label=Extracted region from pLenti_BE3RA-FNLS-PGK-Puro CDS 529..1215 /label=rAPOBEC1 primer_bind 1009..1029 /label=BE-XTEN_F misc_feature complement(1196..1215) /label=APOBEC_R misc_feature 1216..1263 /label=XTEN linker misc_feature 1234..5661 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1510431651498.1 misc_feature complement(1244..1263) /label=BE-XTEN_R CDS 1264..5364 /label=Cas9n (FX) misc_feature 1264..5364 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1507931998114.2 misc_feature 1264..5364 /label=Extracted region from pLentiV_BE3RA misc_feature 1264..2054 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1506995392100.5 misc_feature 1264..1511 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177754792.0 misc_feature 1264..1511 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1502396267528.0 misc_feature 1288..1290 /label=Misc_Feature_4 misc_feature 1512..5364 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177754816.1 misc_feature 1512..5364 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1502396267567.1 misc_feature 2055..3476 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1506995391895.0 primer_bind 3087..3106 /label=Cas9_RA5_F primer_bind 3090..3109 /label=UGIrepair_F misc_feature 3477..3680 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1506995391925.1 misc_feature 3681..3710 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1506995391979.2 misc_feature 3711..4922 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1506995392034.3 misc_feature complement(4566..4585) /label=Cas9_RA5_R primer_bind 4566..4585 /label=BE3_RA3_F and Cas9_RA3_F misc_feature 4732..5364 /label=FragmentGENE for Gibson misc_feature complement(4737..4756) /label=UGIrepair_R misc_feature 4923..5364 /label=wAbpZvAQCfCq7+lnnO0bTPVGc7g.1506995392063.4 primer_bind 5345..5364 /label=BE3_RAUGI_F misc_feature complement(5345..5363) /label=BE3_RA3_R CDS 5365..5637 /label=UGI misc_feature 5367 /label=T misc_feature 5370 /label=T misc_feature 5373 /label=T primer_bind complement(5619..5637) /label=UGI_R CDS 5638..5658 misc_feature join(5662..8622,1..5661) /label=CCGGTCATCATCACCATCACCATTGAGTTT misc_feature 5662..9025 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177991461.16 misc_feature 5662..8749 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496178408388.2 misc_feature 5662..8749 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242743758.18 misc_feature 5662..6058 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177991427.13 misc_feature 5662..6058 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496178099643.26 misc_feature 5662..5945 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242508397.8 CDS complement(5677..5694) /codon_start=1 /label=BGH_rev_primer /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR YTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSTNLSDIIEKETGKQLVIQESIL MLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIK MLSGGSPKKKRKV" CDS 5680..5907 /codon_start=1 /label=bGH_PA_terminator /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR YTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSTNLSDIIEKETGKQLVIQESIL MLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIK MLSGGSPKKKRKV" misc_feature 5946..6058 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242508405.9 CDS complement(5974..5992) /codon_start=1 /label=M13_reverse_primer /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR YTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSTNLSDIIEKETGKQLVIQESIL MLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIK MLSGGSPKKKRKV" CDS complement(5991..6013) /codon_start=1 /label=M13_pUC_rev_primer /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR YTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSTNLSDIIEKETGKQLVIQESIL MLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIK MLSGGSPKKKRKV" CDS complement(6027..6056) /codon_start=1 /label=lac_promoter /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR YTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSTNLSDIIEKETGKQLVIQESIL MLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIK MLSGGSPKKKRKV" misc_feature 6059..8165 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177991434.14 misc_feature 6059..8165 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496178099648.27 misc_feature 6059..8133 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242508413.10 CDS complement(7139..7999) /codon_start=1 /gene="Ampicillin" /label=Ampicillin /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR YTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSTNLSDIIEKETGKQLVIQESIL MLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIK MLSGGSPKKKRKV" CDS complement(7139..7999) /codon_start=1 /label=ORF frame 3 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" CDS complement(8041..8069) /codon_start=1 /label=AmpR_promoter /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 8134..8165 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242508422.11 misc_feature 8166..9025 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496177991440.15 misc_feature 8166..9025 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1496178099654.28 misc_feature 8166..8171 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242508431.12 misc_feature 8172..9025 /label=D8LTms/vLg0Dy7PGNH1wdT/9/E4.1498242508439.13 misc_feature 8372..8952 /label=CMV early promoter misc_feature 8449..8742 /label=CAG enhancer ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggactataag 421 gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga cgataagatg 481 gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccat gagctcagag 541 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag 601 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg 661 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc 721 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt 781 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg 841 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac 901 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg 961 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa 1021 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc 1081 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca 1141 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg 1201 gccaccgggt tgaaaagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa 1261 agtgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg 1321 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg 1381 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag 1441 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc 1501 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga 1561 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc 1621 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag 1681 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac 1741 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac 1801 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc 1861 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga 1921 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac 1981 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag 2041 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc 2101 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc 2161 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct 2221 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg 2281 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc 2341 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg 2401 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg 2461 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac 2521 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc 2581 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc 2641 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa 2701 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag 2761 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg 2821 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg 2881 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc 2941 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc 3001 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt 3061 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg 3121 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc 3181 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc 3241 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg 3301 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac 3361 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg 3421 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca 3481 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg 3541 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga 3601 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacaccca 3661 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg 3721 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat 3781 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc 3841 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag 3901 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg 3961 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag 4021 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac 4081 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc 4141 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac 4201 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag 4261 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag 4321 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc 4381 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg 4441 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt 4501 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg 4561 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc 4621 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc 4681 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg 4741 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac 4801 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag 4861 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg 4921 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc 4981 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa 5041 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg 5101 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag 5161 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc 5221 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa 5281 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc 5341 gacctgtctc agctgggagg cgattcaggc ggatctacta atctgtcaga tattattgaa 5401 aaggagaccg gtaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg 5461 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag 5521 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct 5581 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc 5641 aagaagaaga ggaaagtcta aaaacccgct gatcagcctc gactgtgcct tctagttgcc 5701 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 5761 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 5821 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 5881 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga 5941 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 6001 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 6061 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 6121 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 6181 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 6241 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 6301 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6361 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6541 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6781 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 6841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 7021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 7081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 7141 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7201 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 7261 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 7321 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7381 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7441 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7501 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7561 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7621 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7681 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7741 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7801 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7861 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7921 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7981 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 8041 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 8101 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg 8161 atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc 8221 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa 8281 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 8341 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat 8401 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 8461 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 8521 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 8581 atttacggta aactgcccac ttggcagtac atcaagtgta tc //