LOCUS       Exported               13143 bp ds-DNA     circular SYN 09-MAY-2019
DEFINITION  Gateway destination vector for expressing enhancer-driven Flp1 in 
            Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Poe AR, Tang L, Wang B, Li Y, Sapar ML, Han C.
  TITLE     Dendritic space-filling requires a neuronal type-specific 
            extracellular permissive signal in Drosophila.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 2017;114:E8062-E8071.
  PUBMED    28874572
REFERENCE   2  (bases 1 to 13143)
  AUTHORS   Chun Han
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 9, 2019 from SnapGene 4.3.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13143
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    632..1017
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    1048..2758
                     /label=Gateway RfA cassette
                     /note="Contains attR1 --> attR2"
     protein_bind    1051..1175
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1200..1230
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1284..1943
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2285..2590
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2631..2755)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2772..2974
                     /label=Hsp70
                     /note="TATA box and 5'UTR from Hsp70Bb"
     intron          2978..3039
                     /gene="zeste"
                     /label=zIn
                     /note="2nd intron of Drosophila zeste gene"
     CDS             3059..4330
                     /label=Flippase
                     /note="Flip1 from pJFRC150-20XUAS-IVS-Flp1-PEST, encoding a
                     higher activity Flp with D at AA5."
     misc_feature    4346..4991
                     /label=His2Av stop
                     /note="His2Av stop, including 3'UTR and polyA signal"
     misc_feature    4636..4641
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     protein_bind    4998..5383
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             join(5863..5934,6336..6609,6684..7338,7400..7715,7919..8050,
                     8121..8735)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    9514..10099
                     /label=P element 5' end
     rep_origin      complement(10334..10922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11093..11953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11954..12058)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    12532..12601
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttgggctgca tcacgtaata agtgtgcgtt gaatttattc
      661 gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta tcaaaaaata
      721 agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg tttttaataa
      781 aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa acaaattttc
      841 gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa atacattgca
      901 taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg ttgcataccc
      961 aataaaagat tattatattg catacctttt cttgccatac catttagccg atcaattgtg
     1021 ctcggcaaca gcatgctgca gcagatcatc acaagtttgt acaaaaaagc tgaacgagaa
     1081 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1141 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     1981 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     2041 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     2101 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     2161 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     2221 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     2281 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     2341 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     2401 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     2461 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     2521 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     2581 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     2641 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     2701 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatga
     2761 tctggtctag cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     2821 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     2881 ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     2941 aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca cccttcttaa
     3001 agatcccctt caaagtctaa attcttcctt ttcgtttagc caagactcga gaatcaaaat
     3061 gccgcagttt gatatcctct gcaagacccc accaaaggtg ttggtgcgtc aattcgtgga
     3121 gcgatttgag aggccgtcgg gtgagaagat cgccctgtgc gctgccgagt tgacctattt
     3181 gtgttggatg atcactcata atggcaccgc gattaagcgc gctaccttta tgagctataa
     3241 cactatcatt agcaattccc tgtcctttga catagtaaac aagtccctgc agtttaaata
     3301 caagactcag aaggccacta tattggaggc ttcgctgaaa aagttgatcc cggcatggga
     3361 gttcacgatc atcccatact acggtcagaa acaccagagc gatattaccg atattgtaag
     3421 cagcctccag ctgcagtttg agtccagcga agaggctgat aagggtaata gtcacagcaa
     3481 aaagatgctg aaggcactgc tgtccgaggg cgaaagcatc tgggagatta ctgagaaaat
     3541 cctgaactcg tttgagtaca ccagccgatt caccaagacg aagaccctgt accagttcct
     3601 ctttttggca accttcatca attgtggtcg cttcagtgac atcaaaaacg tggaccctaa
     3661 atcgttcaag ctggtgcaga ataagtacct gggtgttatc atccagtgct tggttaccga
     3721 gactaagacc tccgtgtcca ggcacattta tttcttctcc gcaaggggtc gcatcgatcc
     3781 gctggtatac ttggatgagt tccttcggaa tagtgaacca gtcttgaagc gcgtgaacag
     3841 gacgggcaat tccagtagca acaagcaaga gtaccagctg ctgaaggata atcttgttcg
     3901 gtcgtacaac aaagccttga agaaaaatgc gccatactcg atctttgcaa tcaagaatgg
     3961 tcccaagagt catattggcc ggcacctgat gaccagtttc ttgtccatga agggtctgac
     4021 tgagctcaca aacgtcgttg gcaactggtc ggacaagcga gcgagtgccg tcgcccgcac
     4081 gacatacacc caccagatca cggccatacc agatcactat ttcgcgctgg tgtcgcgtta
     4141 ttatgcctat gatcccatca gcaaggagat gatcgcgctg aaggacgaaa ccaatccaat
     4201 cgaggagtgg cagcatatcg agcaacttaa gggaagcgct gagggtagca tccgttaccc
     4261 cgcctggaac ggcatcatca gccaggaggt tctggattac ctgagctcct acatcaatcg
     4321 ccgtatttaa tctagaatta attaactaag ccagtcggca atcggacgcc ttcgaaacat
     4381 gcaacactaa tgtttaattc agatttcagc agagacaagc taaaacaccg acgagttgta
     4441 atcatttctg tgcgccagca tatatttctt atatacaacg taatacataa ttatgtaatt
     4501 ctagcatctc cccaacactc acatacaaac aaacaaaaaa tacaaacaca caaaacgtat
     4561 ttacccgcac gcatccttgg cgaggttgag tatgaaacaa aaacaaaact taatttagag
     4621 caaagtaatt acacgaataa atttaataaa aaaaaactat aataaaaacg ccaatcatgt
     4681 tatttccaaa aaaaaaaaaa aaaaaaaaac tagacgagat gtttgcgctt gtgtcgatga
     4741 atgtaaagaa ttgcaactac atagatggta attaatttca gctgtaagaa gtacaagttt
     4801 ttcataattg tgagaaaagt gcagtagtat cgcttaaagt tcagttttaa tcagggcatt
     4861 tagcagctcg aacccaatgt atttgtaagc ccaataaacg tgtaaatgcc tagagcaaaa
     4921 tatcacaact gtagcttttc atccgaaaga taataatgaa tagactgacc tgacgttttt
     4981 caaaatgcgt aactagtcac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca
     5041 tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt
     5101 tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac
     5161 cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat
     5221 aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     5281 aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     5341 atattgcata ccttttcttg ccataccatt tagccgatca attctagtat gtatgtaagt
     5401 taataaaacc catttttgcg gaaagtagat aaaaaaaaca tttttttttt ttactgcact
     5461 ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg
     5521 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     5581 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     5641 ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc
     5701 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     5761 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
     5821 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     5881 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
     5941 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
     6001 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc gcacaaacaa
     6061 atagtgacac gaaacagatt attctggtag ctgttctcgc tatataagac aatttttgag
     6121 atcatatcat gatcaagaca tctaaaggca ttcattttcg actatattct tttttacaaa
     6181 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
     6241 aaacctactt cgtaggatac ttcggggtac tttttgttcg gggttagatg agcataacgc
     6301 ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg gcttcgcaga
     6361 gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctccgg ccacccagtc
     6421 cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc tatgcctggc
     6481 acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg cagctggtca
     6541 accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg aaacatttgc
     6601 tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt
     6661 ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt
     6721 gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc
     6781 gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc
     6841 caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct
     6901 aacggccagg gaacacctga ttttccaagc catggtgcgg atgccacgac atctgaccta
     6961 tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca
     7021 gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg
     7081 tctggcattc gcctccgagg ctctaaccga tccgccgctt ctgatctgcg atgagcccac
     7141 ctccggactg gactccttta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca
     7201 gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt ttgagctctt
     7261 tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc
     7321 cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag tattacataa
     7381 catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat ccggcggact
     7441 tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt gatcggatcg
     7501 ccaagatatg cgacaatttt gccattagca aagtagcccg ggatatggag cagttgttgg
     7561 ccaccaaaaa tctggagaag ccactggagc agccggagaa tgggtacacc tacaaggcca
     7621 cctggttcat gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac
     7681 cactcctcgt aaaagtgcga cttattcaga caacggtgag tggttccagt ggaaacaaat
     7741 gatataacgc ttacaattct tggaaacaaa ttcgctagat tttagataga attgcctgat
     7801 tccacaccct tcttagtttt tttcaatgag atgtatagtt tatagttttg cagaagataa
     7861 ataaatttca tttaactcgc gaatattaat gagatgcgag taacatttta atttgcagat
     7921 ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat
     7981 gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc
     8041 cacgataaat gtaagtcatg tttagaatac atttgcattt caataattta ctaactttct
     8101 aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc
     8161 gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattgccgc
     8221 tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc ggactgcggg
     8281 ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa
     8341 cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg
     8401 gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg
     8461 tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc
     8521 tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca
     8581 ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc
     8641 tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc
     8701 tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct
     8761 tgtttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
     8821 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
     8881 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
     8941 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
     9001 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
     9061 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
     9121 acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag
     9181 tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct
     9241 ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact
     9301 ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag
     9361 tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt
     9421 gttgtttata tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa
     9481 tgcttggatt tcactggaac tagaattaat tcggctgctg ctctaaacga cgcatttcgt
     9541 actccaaagt acgaattttt tccctcaagc tcttattttc attaaacaat gaacaggacc
     9601 taacgcacag tcacgttatt gtttacataa atgatttttt ttactattca aacttactct
     9661 gtttgtgtac tcccactggt atagccttct tttatctttt ctggttcagg ctctatcact
     9721 ttactaggta cggcatctgc gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt
     9781 gcagccttcc actgcgaatc attaaagtgg gtatcacaaa tttgggagtt ttcaccaagg
     9841 ctgcacccaa ggctctgctc ccacaatttt ctcttaatag cacacttcgg cacgtgaatt
     9901 aattttactc cagtcacagc tttgcagcaa aatttgcaat atttcatttt tttttattcc
     9961 acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa
    10021 gcaaacgtgc actgaattta agtgtatact tcggtaagct tcggctatcg acgggaccac
    10081 cttatgttat ttcatcatgg gccagaccca cgtagtccag cggcagatcg gcggcggaga
    10141 agttaagcgt ctccaggatg accttgcccg aactggggca cgtggtgttc gacgatgtgc
    10201 agctaatttc gcccggctcc acgtccgccc attggttaat cagcagaccc tcgttggcgt
    10261 aacggaacca tgagaggtac gacaaccatt tgaggtatac tggcaccgag cccgagttca
    10321 agaagaaggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10501 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
    10561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10741 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10861 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10921 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10981 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11041 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11101 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11161 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11221 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11281 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11341 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11401 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11461 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11521 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11581 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11641 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11701 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11761 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11821 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11881 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11941 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12001 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12061 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    12121 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    12181 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    12241 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    12301 atgcggcatc agagcagatt gtactgagag tgcaccattc gacatgcccg ccgtgaccgt
    12361 cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg cacgtcccgt
    12421 gctcaccgtg accaccgcgc ccagcggttt cgagggcgag ggcttcccgg tgcgccgcgc
    12481 gttcgccggg atcaactacc gccacctcga cccgttcatc atgatggacc agatgggtga
    12541 ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc gcggcttcga
    12601 gaccgtgacc tacatcgtcg atatgcggtg tgaaataccg caccgaatcg cgcggaacta
    12661 acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga
    12721 gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg
    12781 cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag
    12841 tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct
    12901 acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat
    12961 tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta
    13021 ataatggtaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc
    13081 gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca
    13141 gac
//
