LOCUS       Exported                4126 bp ds-DNA     circular SYN 27-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     >Cherry in bluescript
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3815..3817
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="color: #ffc0cb"
     misc_feature    2516..2536
                     /locus_tag="generic cas ins up seq"
                     /label=generic cas ins up seq
                     /note="color: #00ffff"
     misc_feature    2588..2593
                     /locus_tag="Kpn!"
                     /label=Kpn!
                     /note="color: #00ffff"
     misc_feature    2609..2614
                     /locus_tag="Sall1"
                     /label=Sall1
                     /note="color: #00ffff"
     misc_feature    2618..2623
                     /locus_tag="ClaI"
                     /label=ClaI
                     /note="color: #ff1b04"
     misc_feature    2624..2629
                     /locus_tag="HindIII"
                     /label=HindIII
                     /note="color: #f7ff58"
     misc_feature    2630..2635
                     /locus_tag="EcoRV"
                     /label=EcoRV
                     /note="color: #00ffff"
     misc_feature    2636..2641
                     /locus_tag="EcoRI"
                     /label=EcoRI
                     /note="color: #ff1b04"
     misc_feature    2642..2695
                     /locus_tag="T2A"
                     /label=T2A
                     /note="color: #2520ff"
     misc_feature    2696..2701
                     /locus_tag="AgeI"
                     /label=AgeI
                     /note="color: #ff44be"
     misc_feature    2702..3097
                     /locus_tag="H2B"
                     /label=H2B
                     /note="color: #00ffff"
     misc_feature    3098..3100
                     /locus_tag="mCherry"
                     /label=mCherry
                     /note="color: #00ffff"
     misc_feature    3098..3100
                     /locus_tag="mCherry(1)"
                     /label=mCherry
                     /label=mCherry(1)
                     /note="color: #00ffff"
     misc_feature    3101..3814
                     /locus_tag="eGFP"
                     /label=eGFP
                     /note="color: #74b81a"
     misc_feature    3574..3592
                     /locus_tag="S32"
                     /label=S32
                     /note="color: #00ffff"
     misc_feature    3595..3613
                     /locus_tag="S31"
                     /label=S31
                     /note="color: #00ffff"
     misc_feature    3815..3817
                     /locus_tag="GFP1-10"
                     /label=GFP1-10
                     /note="color: #26ff2f"
     misc_feature    3818..3837
                     /locus_tag="EP_T2A H2B_F"
                     /label=EP_T2A H2B_F
                     /note="color: #00ffff"
     misc_feature    3818..3823
                     /locus_tag="BamHI"
                     /label=BamHI
                     /note="color: #ff2d15"
     misc_feature    3824..3829
                     /locus_tag="SpeI"
                     /label=SpeI
                     /note="color: #255bff"
     misc_feature    3836..3843
                     /locus_tag="NotI"
                     /label=NotI
                     /note="color: #00ffff"
     misc_feature    3836..3843
                     /locus_tag="New Feature"
                     /label=New Feature
                     /note="color: #2a3dff"
     misc_feature    3854..3859
                     /locus_tag="SacI"
                     /label=SacI
                     /note="color: #fffd1f"
ORIGIN
        1 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
       61 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      121 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      181 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      241 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
      301 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      361 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      421 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      481 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      541 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      601 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      661 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      721 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      781 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      841 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      901 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      961 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1021 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1081 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1141 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1201 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1261 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1321 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1381 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1441 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1501 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1561 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1621 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1681 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1741 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1801 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1861 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1921 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1981 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2041 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2101 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2161 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2221 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2281 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2341 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2401 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2461 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2521 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     2581 aaagctgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2641 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaccgg
     2701 tatgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac
     2761 taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat
     2821 ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat
     2881 gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg
     2941 cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg
     3001 cctgctgctg cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac
     3061 caagtacacc agcgctaagg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     3121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3541 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     3601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3781 cgggatcact ctcggcatgg acgagctgta caagtaggga tccactagtt ctagagcggc
     3841 cgccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     3901 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3961 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4021 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4081 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccag
//
