LOCUS       pRPS-ABE7.10S(D1       19572 bp ds-DNA     circular     14-JUN-2018
DEFINITION  Cloning vector pKI1_1R DNA, complete sequence.
ACCESSION   LC189566 (modified)
VERSION     LC189566.1
KEYWORDS    .
SOURCE      Cloning vector pKI1_1R
  ORGANISM  Cloning vector pKI1_1R other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 18522)
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2016) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroki Tsutsui Nagoya University, Graduate School of
            Science; 1 Furo-cho, Nagoya, Aichi 464-8602, Japan
REFERENCE   2
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene
            knockout in Arabidopsis thaliana
  JOURNAL   Plant Cell Physiol. (2016) In press
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..19572
                     /db_xref="taxon:1907938"
                     /mol_type="other DNA"
                     /organism="Cloning vector pKI1_1R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="source:Cloning vector pKI1_1R"
     primer_bind     31..49
                     /Sequence="GCGGGATATCACAAGTTTG"
                     /Hairpin_Tm="None"
                     /Tm="54.2"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /modified_by="User"
                     /label="CL314"
     regulatory      76..518
                     /note="AtU6.26 promoter"
                     /regulatory_class="promoter"
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AtU6.26 promoter"
     misc_feature    519
                     /note="initial G for transcription"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     unsure          520..538
                     /Color="#ff7400"
                     /created_by="User"
                     /label="oJY276+277(FT)"
     misc_feature    539..614
                     /note="sgRNA_scaffold"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="sgRNA_scaffold"
     regulatory      615..625
                     /regulatory_class="terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     regulatory      634..2297
                     /note="AtRPS5A promoter"
                     /regulatory_class="promoter"
                     /ApEinfo_fwdcolor="#66ffff"
                     /ApEinfo_revcolor="#66ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AtRPS5A promoter"
     misc_feature    1034..1039
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    1066..1073
                     /Recognition_pattern="TTAAT^TAA"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     gene            2303..3493
                     /created_by="User"
                     /label="ABE At opti 7.10"
     unsure          2303..7630
                     /created_by="User"
                     /modified_by="User"
                     /label="SNUBE3 all"
     gene            3494..7606
                     /created_by="User"
                     /modified_by="User"
                     /label="SNUBE3"
     misc_feature    3519
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     unsure          7607..7627
                     /Color="#9911ff"
                     /created_by="User"
                     /label="SV40NLS"
     regulatory      7640..7889
                     /note="Heat_shock_protein18.2 terminator"
                     /regulatory_class="terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Heat_shock_protein18.2 terminator"
     misc_feature    7889..7896
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     misc_feature    7935..11015
                     /note="OLE1-TagRFP cassette"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10940..11015
                     /note="sgRNA_scaffold"
                     /ApEinfo_label="sgRNA_scaffold"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="sgRNA_scaffold(1)"
     misc_feature    11115..11139
                     /note="Right Border"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     unsure          complement(16335..17126)
                     /created_by="User"
                     /Color="#19a0ff"
                     /modified_by="User"
                     /label="SmR"
     misc_feature    17654..17679
                     /note="Left Border"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN      
        1 cgacgtcgca tgctcccggc cgccatggcc gcgggatatc acaagtttgt acaaaaaagc
       61 aggctccgcg gccgctcgtt gaacaacgga aactcgactt gccttccgca caatacatca
      121 tttcttctta gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt
      181 acattttctt gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg
      241 tatcttgttt catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt
      301 tgattgaata aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg
      361 aatctgaaag aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat
      421 aggcccattt aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga
      481 agctgagttt atatacagct agagtcgaag tagtgattgg agatattctc ggaggtgagt
      541 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      601 caccgagtcg gtgctttttt tttttgcggc cgcgatccct caacttttga ttcgctattt
      661 gcagtgcacc tgtggcgttc atcacatctt ttgtgacact gtttgcactg gtcattgcta
      721 ttacaaagga ccttcctgat gttgaaggag atcgaaagta agtaactgca cgcataacca
      781 ttttctttcc gctctttggc tcaatccatt tgacagtcaa agacaatgtt taaccagctc
      841 cgtttgatat attgtcttta tgtgtttgtt caagcatgtt tagttaatca tgcctttgat
      901 tgatcttgaa taggttccaa atatcaaccc tggcaacaaa acttggagtg agaaacattg
      961 cattcctcgg ttctggactt ctgctagtaa attatgtttc agccatatca ctagctttct
     1021 acatgcctca ggtgaattca tctatttccg tcttaactat ttcggttaat taaagcacga
     1081 acaccattac tgcatgtaga agcttgataa actatcgcca ccaatttatt tttgttgcga
     1141 tattgttact ttcctcagta tgcagctttg aaaagaccaa ccctcttatc ctttaacaat
     1201 gaacaggttt ttagaggtag cttgatgatt cctgcacatg tgatcttggc ttcaggctta
     1261 attttccagg taaagcatta tgagatactc ttatatctct tacatacttt tgagataatg
     1321 cacaagaact tcataactat atgctttagt ttctgcattt gacactgcca aattcattaa
     1381 tctctaatat ctttgttgtt gatctttggt agacatgggt actagaaaaa gcaaactaca
     1441 ccaaggtaaa atacttttgt acaaacataa actcgttatc acggaacatc aatggagtgt
     1501 atatctaacg gagtgtagaa acatttgatt attgcaggaa gctatctcag gatattatcg
     1561 gtttatatgg aatctcttct acgcagagta tctgttattc cccttcctct agctttcaat
     1621 ttcatggtga ggatatgcag ttttctttgt atatcattct tcttcttctt tgtagcttgg
     1681 agtcaaaatc ggttccttca tgtacataca tcaaggatat gtccttctga atttttatat
     1741 cttgcaataa aaatgcttgt accaattgaa acaccagctt tttgagttct atgatcactg
     1801 acttggttct aaccaaaaaa aaaaaatgtt taatttacat atctaaaagt aggtttaggg
     1861 aaacctaaac agtaaaatat ttgtatatta ttcgaatttc actcatcata aaaacttaaa
     1921 ttgcaccata aaattttgtt ttactattaa tgatgtaatt tgtgtaactt aagataaaaa
     1981 taatattccg taagttaacc ggctaaaacc acgtataaac cagggaacct gttaaaccgg
     2041 ttctttactg gataaagaaa tgaaagccca tgtagacagc tccattagag cccaaaccct
     2101 aaatttctca tctatataaa aggagtgaca ttagggtttt tgttcgtcct cttaaagctt
     2161 ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag gtgatcttct
     2221 ttctccaaat cctctctcat aactctgatt tcgtacttgt gtatttgagc tcacgctctg
     2281 tttctctcac cacagcccca tgatgagcga ggtcgagttt agccatgaat actggatgag
     2341 acatgctctt actttagcga aacgtgcgtg ggacgagaga gaggttccgg tgggcgcggt
     2401 gttagtacac aataacaggg ttatcggcga agggtggaac aggcctattg gcaggcacga
     2461 tccgactgca catgcggaga tcatggcgct acgacagggg gggctcgtca tgcagaatta
     2521 tcgattgata gacgccacgc tgtacgtcac tctggaacca tgcgtaatgt gtgcaggagc
     2581 gatgatccac tctagaattg gaagagtagt cttcggcgcg agggatgcaa agaccggggc
     2641 ggcggggtcc ctaatggacg tgcttcacca ccctggcatg aaccatagag tggagatcac
     2701 cgagggcata cttgctgatg aatgtgcagc acttctatcc gatttctttc gaatgagaag
     2761 gcaggaaatt aaagcccaga agaaggctca atctagtaca gactctggag ggtccagtgg
     2821 cggctcatcc gggagcgaaa caccagggac gtcagaatcc gctacgcccg aatctagtgg
     2881 tggctccagc ggaggaagta gtgaggtgga gttctctcac gaatactgga tgcgacatgc
     2941 tctaacgcta gcaaaacgag cgagggatga acgagaggtt cctgtaggag cagtgttggt
     3001 tctgaacaac agagttattg gtgaaggttg gaatcgtgct attgggcttc acgacccaac
     3061 agcccatgcc gaaataatgg cgctcaggca aggaggctta gtaatgcaaa actacagatt
     3121 aatcgacgcg accctgtatg tcacgttcga gccatgcgtt atgtgcgcgg gcgcgatgat
     3181 tcattctaga attggaaggg ttgtttttgg ggtgagaaat gccaaaactg gtgcggctgg
     3241 gagtcttatg gacgttctcc actaccctgg tatgaaccat cgagtggaaa tcacggaggg
     3301 gattttggcg gacgaatgtg cagcattgtt atgctatttc ttccgtatgc ctaggcaggt
     3361 cttcaacgct cagaagaagg cgcaaagttc aaccgattca ggggggtctt ctggcggatc
     3421 ttctggttca gagacgcccg gtacctcaga atcagctacg cctgagagtt caggtgggtc
     3481 ttctggcggc agcgacaaga agtacagcat cggcctggcc atcggtacca acagcgtggg
     3541 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aagttcaagg tgctgggcaa
     3601 caccgaccgc cacagcatca agaagaacct gatcggcgcc ctgctgttcg acagcggcga
     3661 gaccgccgag gccacccgcc tgaagcgcac cgcccgccgc cgctacaccc gccgcaagaa
     3721 ccgcatctgc tacctgcagg agatcttcag caacgagatg gccaaggtgg acgacagctt
     3781 cttccaccgc ctggaggaga gcttcctggt ggaggaggac aagaagcacg agcgccaccc
     3841 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     3901 tctgcgcaag aagctggtgg acagcaccga caaggccgac ctgcgcctga tctacctggc
     3961 cctggcccac atgatcaagt tccgcggcca cttcctgatc gagggcgacc tgaaccccga
     4021 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     4081 ggagaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgagcg cccgcctgag
     4141 caagagccgc cgcctggaga acctgatcgc ccagctgccc ggcgagaaga agaacggcct
     4201 gttcggcaac ctgatcgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     4261 cctggccgag gacgccaagc tgcagctgag caaggacacc tacgacgacg acctggacaa
     4321 cctgctggcc cagatcggcg accagtacgc cgacctgttc ctggccgcca agaacctgag
     4381 cgacgccatc ctgctgagcg acatcctgcg cgtgaacacc gagatcacca aggcccccct
     4441 gagcgccagc atgatcaagc gctacgacga gcaccaccag gacctgaccc tgctgaaggc
     4501 cctggtgcgc cagcagctgc ccgagaagta caaggagatc ttcttcgacc agagcaagaa
     4561 cggctacgcc ggctacatcg acggcggcgc cagccaggag gagttctaca agttcatcaa
     4621 gcccatcctg gagaagatgg acggcaccga ggagctgctg gtgaagctga accgcgagga
     4681 cctgctgcgc aagcagcgca ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     4741 cgagctgcac gccatcctgc gccgccagga ggacttctac cccttcctga aggacaaccg
     4801 cgagaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ccctggcccg
     4861 cggcaacagc cgcttcgcct ggatgacccg caagagcgag gagaccatca ccccctggaa
     4921 cttcgaggag gtggtggaca agggcgccag cgcccagagc ttcatcgagc gcatgaccaa
     4981 cttcgacaag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     5041 cttcaccgtg tacaacgagc tgaccaaggt gaagtacgtg accgagggca tgcgcaagcc
     5101 cgccttcctg agcggcgagc agaagaaggc catcgtggac ctgctgttca agaccaaccg
     5161 caaggtgacc gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgacag
     5221 cgtggagatc agcggcgtgg aggaccgctt caacgccagc ctgggcacct accacgacct
     5281 gctgaagatc atcaaggaca aggacttcct ggacaacgag gagaacgagg acatcctgga
     5341 ggacatcgtg ctgaccctga ccctgttcga ggaccgcgag atgatcgagg agcgcctgaa
     5401 gacctacgcc cacctgttcg acgacaaggt gatgaagcag ctgaagcgcc gccgctacac
     5461 cggctggggc cgcctgagcc gcaagcttat caacggcatc cgcgacaagc agagcggcaa
     5521 gaccatcctg gacttcctga agagcgacgg cttcgccaac cgcaacttca tgcagctgat
     5581 ccacgacgac agcctgacct tcaaggagga catccagaag gcccaggtga gcggccaggg
     5641 cgacagcctg cacgagcaca tcgccaacct ggccggcagc cccgccatca agaagggcat
     5701 cctgcagacc gtgaaggtgg tggacgagct ggtgaaggtg atgggccgcc acaagcccga
     5761 gaacatcgtg atcgagatgg cccgcgagaa ccagaccacc cagaagggcc agaagaacag
     5821 ccgcgagcgc atgaagcgca tcgaggaggg catcaaggag ctgggcagcc agatcctgaa
     5881 ggagcacccc gtggagaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     5941 gaacggccgc gacatgtacg tggaccagga gctggacatc aaccgcctga gcgactacga
     6001 cgtggaccac atcgtgcccc agagcttcct gaaggacgac agcatcgaca acaaggtgct
     6061 gacccgcagc gacaagaacc gcggcaagag cgacaacgtg cccagcgagg aggtggtgaa
     6121 gaagatgaag aactactggc gccagctgct gaacgccaag ctgatcaccc agcgcaagtt
     6181 cgacaacctg accaaggccg agcgcggcgg cctgagcgag ctggacaagg ccggcttcat
     6241 caagcgccag ctggtggaga cccgccagat caccaagcac gtggcccaga tcctggacag
     6301 ccgcatgaac accaagtacg acgagaacga caagctgatc cgcgaggtga aggtgatcac
     6361 cctgaagagc aagctggtga gcgacttccg caaggacttc cagttctaca aggtgcgcga
     6421 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtggtgg gcaccgccct
     6481 gatcaagaag taccccaagc tggagagcga gttcgtgtac ggcgactaca aggtgtacga
     6541 cgtgcgcaag atgatcgcca agagcgagca ggagatcggc aaggccaccg ccaagtactt
     6601 cttctacagc aacatcatga acttcttcaa gaccgagatc accctggcca acggcgagat
     6661 ccgcaagcgc cccctgatcg agaccaacgg cgagaccggc gagatcgtgt gggacaaggg
     6721 ccgcgacttc gccaccgtgc gcaaggtgct gagcatgccc caggtgaaca tcgtgaagaa
     6781 gaccgaggtg cagaccggcg gcttcagcaa ggagagcatc ctgcccaagc gcaacagcga
     6841 caagctgatc gcccgcaaga aggactggga ccccaagaag tacggcggct tcgacagccc
     6901 caccgtggcc tacagcgtgc tggtggtggc caaggtggag aagggcaaga gcaagaagct
     6961 gaagagcgtg aaggagctgc tgggcatcac catcatggag cgcagcagct tcgagaagaa
     7021 ccccatcgac ttcctggagg ccaagggcta caaggaggtg aagaaggacc tgatcatcaa
     7081 gctgcccaag tacagcctgt tcgagctgga gaacggccgc aagcgcatgc tggccagcgc
     7141 cggcgagctg cagaagggca acgagctggc cctgcccagc aagtacgtga acttcctgta
     7201 cctggccagc cactacgaga agctgaaggg cagccccgag gacaacgagc agaagcagct
     7261 gttcgtggag cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttcag
     7321 caagcgcgtg atcctggccg acgccaacct ggacaaggtg ctgagcgcct acaacaagca
     7381 ccgcgacaag cccatccgcg agcaggccga gaacatcatc cacctgttca ccctgaccaa
     7441 cctgggcgcc cccgccgcct tcaagtactt cgacaccacc atcgaccgca agcgctacac
     7501 cagcaccaag gaggtgctgg acgccaccct gatccaccag agcatcaccg gtctgtacga
     7561 gacccgcatc gacctgagcc agctgggcgg cgactctggt ggttctccaa agaaaaagag
     7621 aaaagtataa aagggtggga tatgaagatg aagatgaaat atttggtgtg tcaaataaaa
     7681 agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt
     7741 tttctaatat taaatgaatg taagatctca ttataatgaa taaacaaatg tttctataat
     7801 ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt
     7861 atggactatg gaatatgatt aaagataagg cgcgccgacc cagctttctt gtacaaagtg
     7921 gtgatatcac tagtgtatgt aggtatagta acatgaacaa gaacagactc aagtacaaga
     7981 tcgcatacga aaatggaaat ggcaatgtca cttccacata atcaaacacg aatcctcata
     8041 tcaacaaggc ctgagattct aactagctca taacaactta gccaatagtt acttgagact
     8101 accaaatgta tgtagaacta aagactaagg gacagagagt tcgtctaaac aggtgaatct
     8161 agtcgttgtt atctaataaa caattcagcc ccaaatgcag aacacacata gagctctcta
     8221 ttgattcaaa ttacgatctg atactgataa cgtctagatt tttagggtta aagcaatcaa
     8281 tcacctgacg attcaaggtg gttggatcat gacgattcca gaaaacatca agcaagctct
     8341 caaagctaca ctctttggga tcatactgaa ctctaacaac ctcgttatgt cccgtagtgc
     8401 cagtacagac atcctcgtaa ctcggattgt gcacgatgcc atggctatac ccaacctcgg
     8461 tcttggtcac accaggaact ctctggtaag ctagctccac tccccagaaa caaccggcgc
     8521 caaattgcgc gaattgctga cctgaagacg gaacatcatc gtcgggtcct tgggcgattg
     8581 cggcggaaga tgggtcagct tgggcttgag gacgagaccc gaatccgagt ctgttgaaaa
     8641 ggttgttcat tggggatttg tatacggaga ttggtcgtcg agaggtttga gggaaaggac
     8701 aaatgggttt ggctctggag aaagagagtg cggctttaga gagagaattg agaggtttag
     8761 agagagatgc ggcggcgatg agcggaggag agacgacgag gacctgcatt atcaaagcag
     8821 tgacgtggtg aaatttggaa cttttaagag gcagatagat ttattatttg tatccatttt
     8881 cttcattgtt ctagaatgtc gcggaacaaa ttttaaaact aaatcctaaa tttttctaat
     8941 tttgttgcca atagtggata tgtgggccgt atagaaggaa tctattgaag gcccaaaccc
     9001 atactgacga gcccaaaggt tcgttttgcg ttttatgttt cggttcgatg ccaacgccac
     9061 attctgagct aggcaaaaaa caaacgtgtc tttgaataga ctcctctcgt taacacatgc
     9121 agcggctgca tggtgacgcc attaacacgt ggcctacaat tgcatgatgt ctccattgac
     9181 acgtgacttc tcgtctcctt tcttaatata tctaacaaac actcctacct cttccaaaat
     9241 atatacacat ctttttgatc aatctctcat tcaaaatctc attctctcta gtaaacaaga
     9301 acaaaaaaat ggcggataca gctagaggaa cccatcacga tatcatcggc agagaccagt
     9361 acccgatgat gggccgagac cgagaccagt accagatgtc cggacgagga tctgactact
     9421 ccaagtctag gcagattgct aaagctgcaa ctgctgtcac agctggtggt tccctccttg
     9481 ttctctccag ccttaccctt gttggaactg tcatagcttt gactgttgca acacctctgc
     9541 tcgttatctt cagcccaatc cttgtcccgg ctctcatcac agttgcactc ctcatcaccg
     9601 gttttctttc ctctggaggg tttggcattg ccgctataac cgttttctct tggatttaca
     9661 agtaagcaca catttatcat cttacttcat aattttgtgc aatatgtgca tgcatgtgtt
     9721 gagccagtag ctttggatca atttttttgg tcgaataaca aatgtaacaa taagaaattg
     9781 caaattctag ggaacatttg gttaactaaa tacgaaattt gacctagcta gcttgaatgt
     9841 gtctgtgtat atcatctata taggtaaaat gcttggtatg atacctattg attgtgaata
     9901 ggtacgcaac gggagagcac ccacagggat cagacaagtt ggacagtgca aggatgaagt
     9961 tgggaagcaa agctcaggat ctgaaagaca gagctcagta ctacggacag caacatactg
    10021 gtggggaaca tgaccgtgac cgtactcgtg gtggccagca cactactatg agcgagctga
    10081 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
    10141 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
    10201 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
    10261 gcagcagaac cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
    10321 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
    10381 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
    10441 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccaacaccg
    10501 agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg
    10561 tgggcggggg ccacctgatc tgcaacttca agaccacata cagatccaag aaacccgcta
    10621 agaacctcaa gatgcccggc gtctactatg tggaccacag actggaaaga atcaaggagg
    10681 ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
    10741 ctagcaaact ggggcacaag tgaatcgttc aaacatttgg caataaagtt tcttaagatt
    10801 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
    10861 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
    10921 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
    10981 attatcgcgc gcggtgtcat ctatgttact agatcactag tgcggccgcc tgcaggtcga
    11041 ccatatggga gagctcaagc ttagcttgag cttggatcag attgtcgttt cccgccttca
    11101 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
    11161 ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
    11221 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
    11281 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
    11341 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
    11401 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
    11461 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
    11521 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
    11581 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
    11641 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
    11701 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
    11761 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
    11821 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
    11881 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
    11941 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
    12001 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
    12061 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
    12121 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
    12181 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
    12241 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
    12301 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
    12361 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
    12421 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
    12481 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
    12541 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
    12601 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
    12661 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
    12721 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
    12781 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
    12841 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
    12901 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
    12961 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
    13021 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
    13081 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
    13141 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
    13201 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
    13261 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
    13321 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
    13381 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
    13441 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
    13501 gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
    13561 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
    13621 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
    13681 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
    13741 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
    13801 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
    13861 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
    13921 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
    13981 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
    14041 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
    14101 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
    14161 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
    14221 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
    14281 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
    14341 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
    14401 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
    14461 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
    14521 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
    14581 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
    14641 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
    14701 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
    14761 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
    14821 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
    14881 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
    14941 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
    15001 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
    15061 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    15121 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
    15181 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
    15241 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    15301 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    15361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    15421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    15481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    15541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    15601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    15661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    15721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    15781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    15841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    15901 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    15961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    16021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    16081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    16141 actcacgtta agggattttg gtcatgcatg atatatctcc caatttgtgt agggcttatt
    16201 atgcacgctt aaaaataata aaagcagact tgacctgata gtttggctgt gagcaattat
    16261 gtgcttagtg catctaatcg cttgagttaa cgccggcgaa gcggcgtcgg cttgaacgaa
    16321 tttctagcta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
    16381 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
    16441 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
    16501 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
    16561 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
    16621 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
    16681 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
    16741 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
    16801 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
    16861 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
    16921 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
    16981 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
    17041 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
    17101 acttcggcga tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc
    17161 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
    17221 tgcttggatg cccgaggcat agactgtacc ccaaaaaaac atgtcataac aagaagccat
    17281 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
    17341 tgacggcagt tacgctactt gcattacagc ttacgaaccg aacgaggctt atgtccactg
    17401 ggttcgtgcc cgaattgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
    17461 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
    17521 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
    17581 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    17641 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    17701 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattgaat tatcagcttg
    17761 catgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
    17821 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
    17881 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
    17941 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
    18001 tattgccaaa tgtttgaacg atctgcttga ctctagggaa ttaattcctg aatcactgcg
    18061 accggccctc ccgcgaccca gccgagcgag cttagcgaac tgtggacgag aactgtgcca
    18121 ccaagcgtaa ggccgttctc tcgcatttgc cttgctaggc tcgcgcgagt tgctggctga
    18181 ggcgttctcg aaatcagctc ttgttcggtc ggcatctact ctattccttt gccctcggac
    18241 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
    18301 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
    18361 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
    18421 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccttggc gatcctgcaa
    18481 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
    18541 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
    18601 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aatccgcgtg
    18661 cacgagtgcc ggacttcggg gcagtcctcg cccaaagcat cagctcatcg agagcctgcg
    18721 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
    18781 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
    18841 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat agcatccata gcctccgcga
    18901 ccggctgaag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
    18961 cacggcggga gatgcaatag gtcaggctct cgctgaactc cccaatgtca agcacttccg
    19021 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
    19081 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
    19141 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
    19201 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catagggggg attcgagttg
    19261 agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca
    19321 tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat
    19381 aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg ctcagtggct
    19441 ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc
    19501 ggggtcataa cgtgactccc ttaattctca tgtatgataa ttcgagggta cccggggatc
    19561 ctctagaggg cc
//
