LOCUS       pRPS-ABE7.10S(D1       19573 bp ds-DNA     circular     14-JUN-2018
DEFINITION  Cloning vector pKI1_1R DNA, complete sequence.
ACCESSION   LC189566 (modified)
VERSION     LC189566.1
KEYWORDS    .
SOURCE      Cloning vector pKI1_1R
  ORGANISM  Cloning vector pKI1_1R other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 18522)
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2016) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroki Tsutsui Nagoya University, Graduate School of
            Science; 1 Furo-cho, Nagoya, Aichi 464-8602, Japan
REFERENCE   2
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene
            knockout in Arabidopsis thaliana
  JOURNAL   Plant Cell Physiol. (2016) In press
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..19573
                     /db_xref="taxon:1907938"
                     /mol_type="other DNA"
                     /organism="Cloning vector pKI1_1R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="source:Cloning vector pKI1_1R"
     primer_bind     31..49
                     /Sequence="GCGGGATATCACAAGTTTG"
                     /Hairpin_Tm="None"
                     /Tm="54.2"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /modified_by="User"
                     /label="CL314"
     regulatory      76..518
                     /note="AtU6.26 promoter"
                     /regulatory_class="promoter"
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AtU6.26 promoter"
     misc_feature    519
                     /note="initial G for transcription"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    520..538
                     /Original_Bases="AAATGCAGGTGATGACTCACCTGCATA"
                     /label="AAATGCAGGTGATGACTCACCTGCATA"
                     /note="Geneious type: Editing History Replacement"
     unsure          520..539
                     /Color="#ff7400"
                     /created_by="User"
                     /label="oJY300+301(PDS3-2)"
     misc_feature    540..615
                     /note="sgRNA_scaffold"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="sgRNA_scaffold"
     regulatory      616..626
                     /regulatory_class="terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     regulatory      635..2298
                     /note="AtRPS5A promoter"
                     /regulatory_class="promoter"
                     /ApEinfo_fwdcolor="#66ffff"
                     /ApEinfo_revcolor="#66ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AtRPS5A promoter"
     misc_feature    1035..1040
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    1067..1074
                     /Recognition_pattern="TTAAT^TAA"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     gene            2304..3494
                     /created_by="User"
                     /label="ABE At opti 7.10"
     unsure          2304..7631
                     /created_by="User"
                     /modified_by="User"
                     /label="SNUBE3 all"
     gene            3495..7607
                     /created_by="User"
                     /modified_by="User"
                     /label="SNUBE3"
     misc_feature    3520
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     unsure          7608..7628
                     /Color="#9911ff"
                     /created_by="User"
                     /label="SV40NLS"
     regulatory      7641..7890
                     /note="Heat_shock_protein18.2 terminator"
                     /regulatory_class="terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Heat_shock_protein18.2 terminator"
     misc_feature    7890..7897
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     misc_feature    7936..11016
                     /note="OLE1-TagRFP cassette"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10941..11016
                     /note="sgRNA_scaffold"
                     /ApEinfo_label="sgRNA_scaffold"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="sgRNA_scaffold(1)"
     misc_feature    11116..11140
                     /note="Right Border"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     unsure          complement(16336..17127)
                     /created_by="User"
                     /Color="#19a0ff"
                     /modified_by="User"
                     /label="SmR"
     misc_feature    17655..17680
                     /note="Left Border"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN      
        1 cgacgtcgca tgctcccggc cgccatggcc gcgggatatc acaagtttgt acaaaaaagc
       61 aggctccgcg gccgctcgtt gaacaacgga aactcgactt gccttccgca caatacatca
      121 tttcttctta gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt
      181 acattttctt gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg
      241 tatcttgttt catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt
      301 tgattgaata aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg
      361 aatctgaaag aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat
      421 aggcccattt aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga
      481 agctgagttt atatacagct agagtcgaag tagtgattgc ctccagatag ctgcatggag
      541 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      601 gcaccgagtc ggtgcttttt ttttttgcgg ccgcgatccc tcaacttttg attcgctatt
      661 tgcagtgcac ctgtggcgtt catcacatct tttgtgacac tgtttgcact ggtcattgct
      721 attacaaagg accttcctga tgttgaagga gatcgaaagt aagtaactgc acgcataacc
      781 attttctttc cgctctttgg ctcaatccat ttgacagtca aagacaatgt ttaaccagct
      841 ccgtttgata tattgtcttt atgtgtttgt tcaagcatgt ttagttaatc atgcctttga
      901 ttgatcttga ataggttcca aatatcaacc ctggcaacaa aacttggagt gagaaacatt
      961 gcattcctcg gttctggact tctgctagta aattatgttt cagccatatc actagctttc
     1021 tacatgcctc aggtgaattc atctatttcc gtcttaacta tttcggttaa ttaaagcacg
     1081 aacaccatta ctgcatgtag aagcttgata aactatcgcc accaatttat ttttgttgcg
     1141 atattgttac tttcctcagt atgcagcttt gaaaagacca accctcttat cctttaacaa
     1201 tgaacaggtt tttagaggta gcttgatgat tcctgcacat gtgatcttgg cttcaggctt
     1261 aattttccag gtaaagcatt atgagatact cttatatctc ttacatactt ttgagataat
     1321 gcacaagaac ttcataacta tatgctttag tttctgcatt tgacactgcc aaattcatta
     1381 atctctaata tctttgttgt tgatctttgg tagacatggg tactagaaaa agcaaactac
     1441 accaaggtaa aatacttttg tacaaacata aactcgttat cacggaacat caatggagtg
     1501 tatatctaac ggagtgtaga aacatttgat tattgcagga agctatctca ggatattatc
     1561 ggtttatatg gaatctcttc tacgcagagt atctgttatt ccccttcctc tagctttcaa
     1621 tttcatggtg aggatatgca gttttctttg tatatcattc ttcttcttct ttgtagcttg
     1681 gagtcaaaat cggttccttc atgtacatac atcaaggata tgtccttctg aatttttata
     1741 tcttgcaata aaaatgcttg taccaattga aacaccagct ttttgagttc tatgatcact
     1801 gacttggttc taaccaaaaa aaaaaaatgt ttaatttaca tatctaaaag taggtttagg
     1861 gaaacctaaa cagtaaaata tttgtatatt attcgaattt cactcatcat aaaaacttaa
     1921 attgcaccat aaaattttgt tttactatta atgatgtaat ttgtgtaact taagataaaa
     1981 ataatattcc gtaagttaac cggctaaaac cacgtataaa ccagggaacc tgttaaaccg
     2041 gttctttact ggataaagaa atgaaagccc atgtagacag ctccattaga gcccaaaccc
     2101 taaatttctc atctatataa aaggagtgac attagggttt ttgttcgtcc tcttaaagct
     2161 tctcgttttc tctgccgtct ctctcattcg cgcgacgcaa acgatcttca ggtgatcttc
     2221 tttctccaaa tcctctctca taactctgat ttcgtacttg tgtatttgag ctcacgctct
     2281 gtttctctca ccacagcccc atgatgagcg aggtcgagtt tagccatgaa tactggatga
     2341 gacatgctct tactttagcg aaacgtgcgt gggacgagag agaggttccg gtgggcgcgg
     2401 tgttagtaca caataacagg gttatcggcg aagggtggaa caggcctatt ggcaggcacg
     2461 atccgactgc acatgcggag atcatggcgc tacgacaggg ggggctcgtc atgcagaatt
     2521 atcgattgat agacgccacg ctgtacgtca ctctggaacc atgcgtaatg tgtgcaggag
     2581 cgatgatcca ctctagaatt ggaagagtag tcttcggcgc gagggatgca aagaccgggg
     2641 cggcggggtc cctaatggac gtgcttcacc accctggcat gaaccataga gtggagatca
     2701 ccgagggcat acttgctgat gaatgtgcag cacttctatc cgatttcttt cgaatgagaa
     2761 ggcaggaaat taaagcccag aagaaggctc aatctagtac agactctgga gggtccagtg
     2821 gcggctcatc cgggagcgaa acaccaggga cgtcagaatc cgctacgccc gaatctagtg
     2881 gtggctccag cggaggaagt agtgaggtgg agttctctca cgaatactgg atgcgacatg
     2941 ctctaacgct agcaaaacga gcgagggatg aacgagaggt tcctgtagga gcagtgttgg
     3001 ttctgaacaa cagagttatt ggtgaaggtt ggaatcgtgc tattgggctt cacgacccaa
     3061 cagcccatgc cgaaataatg gcgctcaggc aaggaggctt agtaatgcaa aactacagat
     3121 taatcgacgc gaccctgtat gtcacgttcg agccatgcgt tatgtgcgcg ggcgcgatga
     3181 ttcattctag aattggaagg gttgtttttg gggtgagaaa tgccaaaact ggtgcggctg
     3241 ggagtcttat ggacgttctc cactaccctg gtatgaacca tcgagtggaa atcacggagg
     3301 ggattttggc ggacgaatgt gcagcattgt tatgctattt cttccgtatg cctaggcagg
     3361 tcttcaacgc tcagaagaag gcgcaaagtt caaccgattc aggggggtct tctggcggat
     3421 cttctggttc agagacgccc ggtacctcag aatcagctac gcctgagagt tcaggtgggt
     3481 cttctggcgg cagcgacaag aagtacagca tcggcctggc catcggtacc aacagcgtgg
     3541 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaagttcaag gtgctgggca
     3601 acaccgaccg ccacagcatc aagaagaacc tgatcggcgc cctgctgttc gacagcggcg
     3661 agaccgccga ggccacccgc ctgaagcgca ccgcccgccg ccgctacacc cgccgcaaga
     3721 accgcatctg ctacctgcag gagatcttca gcaacgagat ggccaaggtg gacgacagct
     3781 tcttccaccg cctggaggag agcttcctgg tggaggagga caagaagcac gagcgccacc
     3841 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     3901 atctgcgcaa gaagctggtg gacagcaccg acaaggccga cctgcgcctg atctacctgg
     3961 ccctggccca catgatcaag ttccgcggcc acttcctgat cgagggcgac ctgaaccccg
     4021 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     4081 aggagaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgagc gcccgcctga
     4141 gcaagagccg ccgcctggag aacctgatcg cccagctgcc cggcgagaag aagaacggcc
     4201 tgttcggcaa cctgatcgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     4261 acctggccga ggacgccaag ctgcagctga gcaaggacac ctacgacgac gacctggaca
     4321 acctgctggc ccagatcggc gaccagtacg ccgacctgtt cctggccgcc aagaacctga
     4381 gcgacgccat cctgctgagc gacatcctgc gcgtgaacac cgagatcacc aaggcccccc
     4441 tgagcgccag catgatcaag cgctacgacg agcaccacca ggacctgacc ctgctgaagg
     4501 ccctggtgcg ccagcagctg cccgagaagt acaaggagat cttcttcgac cagagcaaga
     4561 acggctacgc cggctacatc gacggcggcg ccagccagga ggagttctac aagttcatca
     4621 agcccatcct ggagaagatg gacggcaccg aggagctgct ggtgaagctg aaccgcgagg
     4681 acctgctgcg caagcagcgc accttcgaca acggcagcat cccccaccag atccacctgg
     4741 gcgagctgca cgccatcctg cgccgccagg aggacttcta ccccttcctg aaggacaacc
     4801 gcgagaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cccctggccc
     4861 gcggcaacag ccgcttcgcc tggatgaccc gcaagagcga ggagaccatc accccctgga
     4921 acttcgagga ggtggtggac aagggcgcca gcgcccagag cttcatcgag cgcatgacca
     4981 acttcgacaa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     5041 acttcaccgt gtacaacgag ctgaccaagg tgaagtacgt gaccgagggc atgcgcaagc
     5101 ccgccttcct gagcggcgag cagaagaagg ccatcgtgga cctgctgttc aagaccaacc
     5161 gcaaggtgac cgtgaagcag ctgaaggagg actacttcaa gaagatcgag tgcttcgaca
     5221 gcgtggagat cagcggcgtg gaggaccgct tcaacgccag cctgggcacc taccacgacc
     5281 tgctgaagat catcaaggac aaggacttcc tggacaacga ggagaacgag gacatcctgg
     5341 aggacatcgt gctgaccctg accctgttcg aggaccgcga gatgatcgag gagcgcctga
     5401 agacctacgc ccacctgttc gacgacaagg tgatgaagca gctgaagcgc cgccgctaca
     5461 ccggctgggg ccgcctgagc cgcaagctta tcaacggcat ccgcgacaag cagagcggca
     5521 agaccatcct ggacttcctg aagagcgacg gcttcgccaa ccgcaacttc atgcagctga
     5581 tccacgacga cagcctgacc ttcaaggagg acatccagaa ggcccaggtg agcggccagg
     5641 gcgacagcct gcacgagcac atcgccaacc tggccggcag ccccgccatc aagaagggca
     5701 tcctgcagac cgtgaaggtg gtggacgagc tggtgaaggt gatgggccgc cacaagcccg
     5761 agaacatcgt gatcgagatg gcccgcgaga accagaccac ccagaagggc cagaagaaca
     5821 gccgcgagcg catgaagcgc atcgaggagg gcatcaagga gctgggcagc cagatcctga
     5881 aggagcaccc cgtggagaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     5941 agaacggccg cgacatgtac gtggaccagg agctggacat caaccgcctg agcgactacg
     6001 acgtggacca catcgtgccc cagagcttcc tgaaggacga cagcatcgac aacaaggtgc
     6061 tgacccgcag cgacaagaac cgcggcaaga gcgacaacgt gcccagcgag gaggtggtga
     6121 agaagatgaa gaactactgg cgccagctgc tgaacgccaa gctgatcacc cagcgcaagt
     6181 tcgacaacct gaccaaggcc gagcgcggcg gcctgagcga gctggacaag gccggcttca
     6241 tcaagcgcca gctggtggag acccgccaga tcaccaagca cgtggcccag atcctggaca
     6301 gccgcatgaa caccaagtac gacgagaacg acaagctgat ccgcgaggtg aaggtgatca
     6361 ccctgaagag caagctggtg agcgacttcc gcaaggactt ccagttctac aaggtgcgcg
     6421 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtggtg ggcaccgccc
     6481 tgatcaagaa gtaccccaag ctggagagcg agttcgtgta cggcgactac aaggtgtacg
     6541 acgtgcgcaa gatgatcgcc aagagcgagc aggagatcgg caaggccacc gccaagtact
     6601 tcttctacag caacatcatg aacttcttca agaccgagat caccctggcc aacggcgaga
     6661 tccgcaagcg ccccctgatc gagaccaacg gcgagaccgg cgagatcgtg tgggacaagg
     6721 gccgcgactt cgccaccgtg cgcaaggtgc tgagcatgcc ccaggtgaac atcgtgaaga
     6781 agaccgaggt gcagaccggc ggcttcagca aggagagcat cctgcccaag cgcaacagcg
     6841 acaagctgat cgcccgcaag aaggactggg accccaagaa gtacggcggc ttcgacagcc
     6901 ccaccgtggc ctacagcgtg ctggtggtgg ccaaggtgga gaagggcaag agcaagaagc
     6961 tgaagagcgt gaaggagctg ctgggcatca ccatcatgga gcgcagcagc ttcgagaaga
     7021 accccatcga cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca
     7081 agctgcccaa gtacagcctg ttcgagctgg agaacggccg caagcgcatg ctggccagcg
     7141 ccggcgagct gcagaagggc aacgagctgg ccctgcccag caagtacgtg aacttcctgt
     7201 acctggccag ccactacgag aagctgaagg gcagccccga ggacaacgag cagaagcagc
     7261 tgttcgtgga gcagcacaag cactacctgg acgagatcat cgagcagatc agcgagttca
     7321 gcaagcgcgt gatcctggcc gacgccaacc tggacaaggt gctgagcgcc tacaacaagc
     7381 accgcgacaa gcccatccgc gagcaggccg agaacatcat ccacctgttc accctgacca
     7441 acctgggcgc ccccgccgcc ttcaagtact tcgacaccac catcgaccgc aagcgctaca
     7501 ccagcaccaa ggaggtgctg gacgccaccc tgatccacca gagcatcacc ggtctgtacg
     7561 agacccgcat cgacctgagc cagctgggcg gcgactctgg tggttctcca aagaaaaaga
     7621 gaaaagtata aaagggtggg atatgaagat gaagatgaaa tatttggtgt gtcaaataaa
     7681 aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct
     7741 ttttctaata ttaaatgaat gtaagatctc attataatga ataaacaaat gtttctataa
     7801 tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg
     7861 tatggactat ggaatatgat taaagataag gcgcgccgac ccagctttct tgtacaaagt
     7921 ggtgatatca ctagtgtatg taggtatagt aacatgaaca agaacagact caagtacaag
     7981 atcgcatacg aaaatggaaa tggcaatgtc acttccacat aatcaaacac gaatcctcat
     8041 atcaacaagg cctgagattc taactagctc ataacaactt agccaatagt tacttgagac
     8101 taccaaatgt atgtagaact aaagactaag ggacagagag ttcgtctaaa caggtgaatc
     8161 tagtcgttgt tatctaataa acaattcagc cccaaatgca gaacacacat agagctctct
     8221 attgattcaa attacgatct gatactgata acgtctagat ttttagggtt aaagcaatca
     8281 atcacctgac gattcaaggt ggttggatca tgacgattcc agaaaacatc aagcaagctc
     8341 tcaaagctac actctttggg atcatactga actctaacaa cctcgttatg tcccgtagtg
     8401 ccagtacaga catcctcgta actcggattg tgcacgatgc catggctata cccaacctcg
     8461 gtcttggtca caccaggaac tctctggtaa gctagctcca ctccccagaa acaaccggcg
     8521 ccaaattgcg cgaattgctg acctgaagac ggaacatcat cgtcgggtcc ttgggcgatt
     8581 gcggcggaag atgggtcagc ttgggcttga ggacgagacc cgaatccgag tctgttgaaa
     8641 aggttgttca ttggggattt gtatacggag attggtcgtc gagaggtttg agggaaagga
     8701 caaatgggtt tggctctgga gaaagagagt gcggctttag agagagaatt gagaggttta
     8761 gagagagatg cggcggcgat gagcggagga gagacgacga ggacctgcat tatcaaagca
     8821 gtgacgtggt gaaatttgga acttttaaga ggcagataga tttattattt gtatccattt
     8881 tcttcattgt tctagaatgt cgcggaacaa attttaaaac taaatcctaa atttttctaa
     8941 ttttgttgcc aatagtggat atgtgggccg tatagaagga atctattgaa ggcccaaacc
     9001 catactgacg agcccaaagg ttcgttttgc gttttatgtt tcggttcgat gccaacgcca
     9061 cattctgagc taggcaaaaa acaaacgtgt ctttgaatag actcctctcg ttaacacatg
     9121 cagcggctgc atggtgacgc cattaacacg tggcctacaa ttgcatgatg tctccattga
     9181 cacgtgactt ctcgtctcct ttcttaatat atctaacaaa cactcctacc tcttccaaaa
     9241 tatatacaca tctttttgat caatctctca ttcaaaatct cattctctct agtaaacaag
     9301 aacaaaaaaa tggcggatac agctagagga acccatcacg atatcatcgg cagagaccag
     9361 tacccgatga tgggccgaga ccgagaccag taccagatgt ccggacgagg atctgactac
     9421 tccaagtcta ggcagattgc taaagctgca actgctgtca cagctggtgg ttccctcctt
     9481 gttctctcca gccttaccct tgttggaact gtcatagctt tgactgttgc aacacctctg
     9541 ctcgttatct tcagcccaat ccttgtcccg gctctcatca cagttgcact cctcatcacc
     9601 ggttttcttt cctctggagg gtttggcatt gccgctataa ccgttttctc ttggatttac
     9661 aagtaagcac acatttatca tcttacttca taattttgtg caatatgtgc atgcatgtgt
     9721 tgagccagta gctttggatc aatttttttg gtcgaataac aaatgtaaca ataagaaatt
     9781 gcaaattcta gggaacattt ggttaactaa atacgaaatt tgacctagct agcttgaatg
     9841 tgtctgtgta tatcatctat ataggtaaaa tgcttggtat gatacctatt gattgtgaat
     9901 aggtacgcaa cgggagagca cccacaggga tcagacaagt tggacagtgc aaggatgaag
     9961 ttgggaagca aagctcagga tctgaaagac agagctcagt actacggaca gcaacatact
    10021 ggtggggaac atgaccgtga ccgtactcgt ggtggccagc acactactat gagcgagctg
    10081 attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
    10141 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
    10201 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
    10261 ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
    10321 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
    10381 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
    10441 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc
    10501 gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc
    10561 gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct
    10621 aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag
    10681 gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
    10741 cctagcaaac tggggcacaa gtgaatcgtt caaacatttg gcaataaagt ttcttaagat
    10801 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
    10861 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
    10921 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
    10981 aattatcgcg cgcggtgtca tctatgttac tagatcacta gtgcggccgc ctgcaggtcg
    11041 accatatggg agagctcaag cttagcttga gcttggatca gattgtcgtt tcccgccttc
    11101 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
    11161 tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
    11221 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
    11281 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
    11341 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
    11401 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
    11461 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
    11521 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
    11581 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
    11641 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
    11701 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
    11761 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
    11821 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
    11881 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
    11941 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
    12001 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
    12061 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
    12121 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
    12181 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
    12241 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
    12301 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
    12361 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
    12421 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
    12481 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
    12541 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
    12601 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
    12661 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
    12721 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
    12781 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
    12841 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
    12901 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
    12961 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
    13021 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
    13081 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
    13141 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
    13201 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
    13261 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
    13321 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
    13381 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
    13441 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
    13501 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
    13561 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
    13621 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
    13681 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
    13741 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
    13801 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
    13861 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
    13921 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
    13981 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
    14041 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
    14101 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
    14161 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
    14221 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
    14281 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
    14341 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
    14401 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
    14461 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
    14521 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
    14581 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
    14641 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    14701 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    14761 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    14821 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
    14881 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    14941 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    15001 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    15061 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    15121 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    15181 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    15241 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    15301 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    15361 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    15421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    15481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    15541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    15601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    15661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    15721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    15781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    15841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    15901 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    15961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    16021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    16081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    16141 aactcacgtt aagggatttt ggtcatgcat gatatatctc ccaatttgtg tagggcttat
    16201 tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta
    16261 tgtgcttagt gcatctaatc gcttgagtta acgccggcga agcggcgtcg gcttgaacga
    16321 atttctagct agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
    16381 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
    16441 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
    16501 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
    16561 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
    16621 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
    16681 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
    16741 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
    16801 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
    16861 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
    16921 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
    16981 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
    17041 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
    17101 tacttcggcg atcaccgctt cccccatgat gtttaacttt gttttagggc gactgccctg
    17161 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
    17221 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa catgtcataa caagaagcca
    17281 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
    17341 gtgacggcag ttacgctact tgcattacag cttacgaacc gaacgaggct tatgtccact
    17401 gggttcgtgc ccgaattgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
    17461 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
    17521 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
    17581 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
    17641 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    17701 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattgaa ttatcagctt
    17761 gcatgccggt cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
    17821 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
    17881 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
    17941 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
    18001 ttattgccaa atgtttgaac gatctgcttg actctaggga attaattcct gaatcactgc
    18061 gaccggccct cccgcgaccc agccgagcga gcttagcgaa ctgtggacga gaactgtgcc
    18121 accaagcgta aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg
    18181 aggcgttctc gaaatcagct cttgttcggt cggcatctac tctattcctt tgccctcgga
    18241 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
    18301 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
    18361 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
    18421 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccttgg cgatcctgca
    18481 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
    18541 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
    18601 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaatccgcgt
    18661 gcacgagtgc cggacttcgg ggcagtcctc gcccaaagca tcagctcatc gagagcctgc
    18721 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
    18781 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
    18841 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tagcatccat agcctccgcg
    18901 accggctgaa gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
    18961 gcacggcggg agatgcaata ggtcaggctc tcgctgaact ccccaatgtc aagcacttcc
    19021 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
    19081 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
    19141 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
    19201 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcataggggg gattcgagtt
    19261 gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc
    19321 atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa
    19381 taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctcagtggc
    19441 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
    19501 cggggtcata acgtgactcc cttaattctc atgtatgata attcgagggt acccggggat
    19561 cctctagagg gcc
//
