LOCUS       pBoL1P4GA\(AddGene\#\112910)        1448 bp    DNA     linear       14-JUN-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12333150|
COMMENT     VNTDBDATE|-12333150|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBoL1P4GA (AddGene # 112910)|
COMMENT     VNTAUTHORNAME|auto|
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FEATURES             Location/Qualifiers
     misc_feature    224..227
                     /vntifkey="21"
                     /label=Splice\to\5'\oligo
     misc_feature    1428..1431
                     /vntifkey="21"
                     /label=Splice\to\L2\vector
     misc_feature    231..1299
                     /vntifkey="21"
                     /label=RFP\selection
     misc_feature    1307..1427
                     /vntifkey="21"
                     /label=sgRNA\missing\1st\4\nts
     misc_feature    18..21
                     /vntifkey="21"
                     /label=3/4\splice
     misc_feature    22..223
                     /vntifkey="21"
                     /label=U6\26\promoter
BASE COUNT      410 a       339 c       346 g       353 t 
ORIGIN
        1 cactctgtgg aagacaatta ccatcttcat tcttaagata tgaagataat cttcaaaagg 
       61 cccctgggaa tctgaaagaa gagaagcagg cccatttata tgggaaagaa caatagtatt 
      121 tcttatatag gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc gcttagataa 
      181 gaaaacgaag ctgagtttat atacagctag agtcgaagta gtgcgagacg caatacgcaa 
      241 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
      301 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 
      361 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 
      421 atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga ggacgttatc 
      481 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 
      541 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 
      601 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 
      661 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 
      721 gaaggtttca aatgggaacg tgttatgaac ttcgaggacg gtggtgttgt taccgttacc 
      781 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 
      841 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 
      901 cgtatgtacc cggaggacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 
      961 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 
     1021 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaggactac 
     1081 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc 
     1141 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 
     1201 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 
     1261 acactggctc accttcgggt gggcctttct gcgtttatac gtctcttaga gctagaaata 
     1321 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 
     1381 tttttctaga cccagctttc ttgtacaaag ttggcattac gctttacggg aaagtcttcc 
     1441 acgaagtg 
//
