LOCUS Exported 4973 bp ds-DNA circular SYN 02-MAY-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 4973) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported May 2, 2018 from SnapGene 4.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4973 /organism="recombinant plasmid" /mol_type="other DNA" primer_bind 625..647 /label=T7 Primer /note="ApEinfo_fwdcolor=pink vntifkey=28 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" misc_feature 690..830 /label=AAV-2 ITR /note="ApEinfo_fwdcolor=pink vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" primer_bind 843..889 /label=SalI_CMV_Fw enhancer 849..1228 /note="CMV enhancer human cytomegalovirus immediate early enhancer" promoter 1229..1432 /note="CMV promoter human cytomegalovirus (CMV) immediate early promoter" promoter 1477..1495 /note="T7 promoter promoter for bacteriophage T7 RNA polymerase" CDS 1515..1748 /codon_start=1 /label=AcrIIA4 /translation="MAPKKKRKVGGSGGSMNINDLIREIKNKDYTVKLSGTDSNSITQL IIRVNNDGNEYVISESENESIVEKFISAFKNGW" CDS 1749..2177 /codon_start=1 /label=AsLOV2 hs /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL" CDS 2178..2243 /codon_start=1 /label=AcrIIA4 /translation="YEDEEEFYNDMQTITLKSELN" polyA_signal 2350..2574 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" misc_feature 2595..2735 /label=AAV-4 ITR /note="ApEinfo_fwdcolor=pink vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" primer_bind 2784..2803 /label=T3 Primer /note="ApEinfo_fwdcolor=pink vntifkey=28 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" CDS complement(3985..4845) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4846..4950) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctctc 661 tagaatgcag gggggggggg gggggggggc cactccctct ctgcgcgctc gctcgctcac 721 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 781 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct agatctgata 841 tcgtcgacga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 901 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 961 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 1021 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 1081 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 1141 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 1201 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 1261 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 1321 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 1381 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct 1441 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac 1501 ccaagctggc tagcatggcc ccgaagaaaa agcgaaaggt gggcggaagc ggaggttcca 1561 tgaacattaa cgacctgatt cgggaaataa agaacaagga ctacacggtc aagctgtcag 1621 gcaccgactc caactctata acacagctca ttataagggt taataatgat ggcaacgaat 1681 atgtcatctc tgaatccgag aatgaatcaa tcgtggagaa attcatcagt gcatttaaaa 1741 acggctggct tgctacaact ttggagcgga ttgagaagaa tttcgtcata actgatccca 1801 ggttgcctga taatcctata atattcgcct ccgatagttt tcttcaactt acggagtact 1861 ccagagagga gatactggga aggaattgca gattcctcca aggtccagaa actgatagag 1921 ctactgtgag gaagatacgg gatgccattg acaatcaaac tgaagtgaca gtgcaactta 1981 tcaattacac caagtcaggg aaaaaattct ggaacctgtt ccatttgcaa cccatgaggg 2041 accaaaaggg cgatgtgcaa tatttcatag gcgttcaact tgatggtact gaacacgtca 2101 gagatgcggc ggagcgagaa ggagtgatgc tgattaagaa aacagcagag aatatcgacg 2161 aagcggcgaa agagttgtac gaagatgagg aagagttcta caatgatatg caaactatca 2221 cactgaaatc tgagctgaac tagctcgagc ggccgccact gtgctggata tctgcagaat 2281 tccaccacac tggactagtg gatccgagct cggtaccaag cttaagttta aaccgctgat 2341 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 2401 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 2461 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 2521 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggggcgcg 2581 ccgatatcag atctggcaaa cctagatgat ggaggtaccc actccctcta tgcgcgctcg 2641 ctcactcact cggccctgcc ggccagaggc cggcagtctg gagacctttg gtctccaggg 2701 ccgagtgagt gagcgagcgc gcatagaggg agtgggtagg acgcgtcctg caggatgcat 2761 actagtggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc 2821 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 2881 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 2941 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3001 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 3061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 3661 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 3901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 3961 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 4021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 4081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 4141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 4201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 4261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 4321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 4381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 4441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 4501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 4561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 4621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 4681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 4741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 4801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 4861 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 4921 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac //