LOCUS       Exported                5285 bp ds-DNA     circular SYN 02-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported May 2, 2018 from SnapGene 4.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2171..2190)
                     /label=T3 Primer
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2239..2379
                     /label=AAV-4 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     promoter        2400..2871
                     /label=U6
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=29
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     promoter        2627..2867
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2878..2896
                     /label=CCR5 gRNA
     misc_feature    2897..2983
                     /label=misc_feature
                     /note="3'overhang=GGCC
                     ugene_name=Fragment 3
                     5'overhang=GTAC
                     3'type=sticky
                     fragment_source=_0443 pBS H1 GFP
                     ugene_group=fragments
                     ApEinfo_fwdcolor=pink
                     3'strand=rev-compl
                     3'term=NotI
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     5'term=KpnI
                     5'strand=rev-compl
                     5'type=sticky"
     misc_feature    2897..2983
                     /label=F+E Sp scaffold
                     /note="ApEinfo_fwdcolor=#0080c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     primer_bind     complement(2965..2989)
                     /label=#843 MH_SpScaffoldFE_XbaI_re
     polyA_signal    complement(2984..2989)
                     /label=6xT
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     polyA_site      complement(3010..3053)
                     /label=minimal polyA
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=26
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     CDS             complement(3060..3794)
                     /codon_start=1
                     /label=gfp
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /translation="TGRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLR
                     QADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRLRPGAHHL
                     LQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAE
                     ERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRVAARQPLPEHPVRPEQRPQREARS
                     HGPAGVRDRRRDHSRHGRAVQVK"
     promoter        complement(3816..4437)
                     /label=RSV
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=29
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    complement(4456..4596)
                     /label=AAV-2 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     complement(4639..4661)
                     /label=T7 Primer
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtatgcatc
     2221 ctgcaggacg cgtcctaccc actccctcta tgcgcgctcg ctcactcact cggccctgga
     2281 gaccaaaggt ctccagactg ccggcctctg gccggcaggg ccgagtgagt gagcgagcgc
     2341 gcatagaggg agtgggtacc tccatcatct aggtttgcca gatctgatat cggcgcgccc
     2401 gagtccaaca cccgtgggaa tcccatgggc accatggccc ctcgctccaa aaatgctttc
     2461 gcgtcgcgca gacactgctc ggtagtttcg gggatcagcg tttgagtaag agcccgcgtc
     2521 tgaaccctcc gcgccgcccc ggccccagtg gaaagacgcg caggcaaaac gcaccacgtg
     2581 acggagcgtg accgcgcgcc gagcgcgcgc caaggtcggg caggaagagg gcctatttcc
     2641 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
     2701 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     2761 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     2821 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgtgac
     2881 atcaattatt atacatgttt aagagctatg ctggaaacag catagcaagt ttaaataagg
     2941 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg cggccgcctc
     3001 gagtgatcaa aaaaccaaca cacgcttcca atgaaaataa acgatccttt attgctagcc
     3061 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     3121 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     3181 agtggttgtc gggcagcaac acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     3241 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     3301 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     3361 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     3421 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     3481 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     3541 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     3601 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     3661 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     3721 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
     3781 tggtggcgac cggtggatcc cgggcccgcg gtacccagct tggaggtgca caccaatgtg
     3841 gtgaatggtc aaatggcgtt tattgtatcg agctaggcac ttaaatacaa tatctctgca
     3901 atgcggaatt cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag
     3961 gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt
     4021 aaggcatgtt gctaactcat ccttaccatg ttgcaagact accaagagta tttgcataag
     4081 actacatttc cccctcccta tgcaaaagcg aaactactat atcctgaggg gactcctaac
     4141 cgcgtacaac cgaagccccg cttttcgcct aaacacaccc tagtcccctc agatacgcgt
     4201 atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat
     4261 gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc
     4321 gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat
     4381 tgtactgaga gtgcaccata cggatctgcg atgataagct gtcaaacatg agaattggtc
     4441 gacgatatca gatctaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4501 gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg
     4561 cctcagtgag cgagcgagcg cgcagagagg gagtggcccc cccccccccc cccccctgca
     4621 ttctagagag ctccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     4681 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4741 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4801 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4861 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4921 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4981 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5041 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5101 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5161 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5221 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5281 tttag
//
