LOCUS       pAAV-hSyn-GRAB-DA1      6349 bp    DNA     circular UNA UNA
FEATURES             Location/Qualifiers
     unsure          1..141
                     /vntifkey=21
                     /label=L-ITR
     promoter        156..597
                     /label=synapsin
     CDS             598..2451
                     /label=GRAB-DA1h
     unsure          2449..2451
                     /label=IgK-DA4.1
     unsure          2466..3074
                     /vntifkey=21
                     /label=WPRE
     polyA_signal    3094..3572
                     /vntifkey=25
                     /label="hGH polyA"
                     /label="PolyA signal"
     unsure          3612..3752
                     /vntifkey=21
                     /label=R-ITR
     rep_origin      3844..4150
                     /vntifkey=33
                     /label="f1 Origin"
                     /label="Replication origin"
     unsure          4669..5526
                     /vntifkey=21
                     /label=AmpR
     rep_origin      5677..6344
                     /vntifkey=33
                     /label="pUC ori"
                     /label="Replication origin"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTATGAG GCGGGGTGGG GGTGCCTACC
      181 TGACGACCGA CCCCGACCCA CTGGACAAGC ACCCAACCCC CATTCCCCAA ATTGCGCATC
      241 CCCTATCAGA GAGGGGGAGG GGAAACAGGA TGCGGCGAGG CGCGTGCGCA CTGCCAGCTT
      301 CAGCACCGCG GACAGTGCCT TCGCCCCCGC CTGGCGGCGC GCGCCACCGC CGCCTCAGCA
      361 CTGAAGGCGC GCTGACGTCA CTCGCCGGTC CCCCGCAAAC TCCCCTTCCC GGCCACCTTG
      421 GTCGCGTCCG CGCCGCCGCC GGCCCAGCCG GACCGCACCA CGCGAGGCGC GAGATAGGGG
      481 GGCACGGGCG CGACCATCTG CGCTGCGGCG CCGGCGACTC AGCGCTGCCT CAGTCTGCGG
      541 TGGGCAGCGG AGGAGTCGTG TCGTGCCTGA GAGCGCAGTC GAGAAACCGG TGCCACCATG
      601 GAGACAGACA CACTCCTGCT ATGGGTACTG CTGCTCTGGG TTCCAGGTTC CACTGGTGAC
      661 ACAAGTTTGT ACAAAAAAGT TGGCACCACC GGTGATCCAC TGAATCTGTC CTGGTATGAT
      721 GATGATCTGG AGAGGCAGAA CTGGAGCCGG CCCTTCAACG GGTCAGACGG GAAGGCGGAC
      781 AGACCCCACT ACAACTACTA TGCCACACTG CTCACCCTGC TCATCGCTGT CATCGTCTTC
      841 GGCAACGTGC TGGTGTGCAT GGCTGTGTCC CGCGAGAAGG CGCTGCAGAC CACCACCAAC
      901 TACCTGATCG TCAGCCTCGC AGTGGCCGAC CTCCTCGTCG CCACACTGGT CATGCCCTGG
      961 GTTGTCTACC TGGAGGTGGT AGGTGAGTGG AAATTCAGCA GGATTCACTG TGACATCTTC
     1021 GTCACTCTGG ACGTCATGAT GTGCACGGCG AGCATCCTGA ACTTGTGTGC CATCAGCATC
     1081 GACAGGTACA CAGCTGTGGC CATGCCCATG CTGTACAATA CGCGCTACAG CTCCAAGCGC
     1141 CGGGTCACCG TCATGATCTC CATCGTCTGG GTCCTGTCCT TCACCATCTC CTGCCCACTC
     1201 CTCTTCGGAC TCAATAACGC AGACCAGAAC GAGTGCATCA TTGCCAACCC GGCCTTCGTG
     1261 GTCTACTCCT CCATCGTCTC CTTCTACGTG CCCTTCATTG TCATGCTGCT GGTCTACATC
     1321 AAGATCTACA TTGTCCTCCG CAGACGCCGC AAGCGAGTCA ACACCAAACG CAGCAGCCGA
     1381 GCTTTCAGGG CCCACCTGAG GGCTCCACTA AAGGGCAACT GTACTCACCC CGAGGACATG
     1441 AAACTCGGAG GAAACGTCTA TATCAAGGCC GACAAGCAGA AGAACGGCAT CAAGGCGAAC
     1501 TTCCACATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCTACCA CTACCAGCAG
     1561 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCGTGCAG
     1621 TCCAAACTTT CGAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
     1681 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC
     1741 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     1801 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GTGAGGGCGA TGCCACCTAC
     1861 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     1921 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
     1981 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACA TCCAGGAGCG CACCATCTTC
     2041 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2101 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2161 AAGCTGGAGT ACAACACTGG AGCAGCAGCA ATGAGCCGTA GGAAGCTCTC CCAGCAGAAG
     2221 GAGAAGAAAG CCACTCAGAT GCTCGCCATT GTTCTCGGCG TGTTCATCAT CTGCTGGCTG
     2281 CCCTTCTTCA TCACACACAT CCTGAACATA CACTGTGACT GCAACATCCC GCCTGTCCTG
     2341 TACAGCGCCT TCACGTGGCT GGGCTATGTC AACAGCGCCG TGAACCCCAT CATCTACACC
     2401 ACCTTCAACA TTGAGTTCCG CAAGGCCTTC CTGAAGATCC TCCACTGCTG AGAATTCGAT
     2461 ATCAAGCTTA TCGATCATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT
     2521 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT
     2581 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT
     2641 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT
     2701 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC
     2761 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG
     2821 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC
     2881 TTTCCTTGGC TGCTCGCCTA TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC
     2941 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG
     3001 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC
     3061 CCGCATCGAT ACCGAGCGCT GCTCGAGAGA TCTACGGGTG GCATCCCTGT GACCCCTCCC
     3121 CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA
     3181 AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG
     3241 GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT
     3301 GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG
     3361 TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA
     3421 GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG
     3481 TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC
     3541 AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT GTAGGTAACC ACGTGCGGAC
     3601 CGAGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC
     3661 GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC
     3721 AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT TTCTCCTTAC
     3781 GCATCTGTGC GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC GCCCTGTAGC
     3841 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC
     3901 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT
     3961 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC
     4021 CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG
     4081 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA
     4141 ACTGGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG
     4201 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC
     4261 AAAATATTAA CGTTTACAAT TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA
     4321 TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG
     4381 CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG
     4441 TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA
     4501 TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT
     4561 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
     4621 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA
     4681 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC
     4741 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
     4801 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
     4861 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC
     4921 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
     4981 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
     5041 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
     5101 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
     5161 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG
     5221 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA
     5281 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG
     5341 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT
     5401 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT
     5461 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG
     5521 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT
     5581 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT
     5641 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT
     5701 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA
     5761 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC
     5821 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC
     5881 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT
     5941 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
     6001 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC
     6061 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG
     6121 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG
     6181 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT
     6241 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC
     6301 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGT
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