LOCUS Exported 5283 bp ds-DNA circular SYN 26-APR-2018 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5283) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Apr 26, 2018 from SnapGene 4.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5283 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 24..128 /gene="bla" /label=AmpR promoter CDS 129..989 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2171..2190) /label=T3 Primer /note="ApEinfo_fwdcolor=pink vntifkey=28 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" misc_feature 2239..2379 /label=AAV-4 ITR /note="ApEinfo_fwdcolor=pink vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" promoter 2400..2871 /label=U6 /note="ApEinfo_fwdcolor=pink vntifkey=29 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" misc_feature 2627..2871 /label=short U6 Variant FZ promoter 2627..2867 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 2644..2675 /label=727 misc_feature 2895..2981 /label=misc_feature /note="3'overhang=GGCC ugene_name=Fragment 3 5'overhang=GTAC 3'type=sticky fragment_source=_0443 pBS H1 GFP ugene_group=fragments ApEinfo_fwdcolor=pink 3'strand=rev-compl 3'term=NotI ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink 5'term=KpnI 5'strand=rev-compl 5'type=sticky" misc_feature 2895..2981 /label=F+E Sp scaffold /note="ApEinfo_fwdcolor=#0080c0 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green" polyA_signal complement(2982..2987) /label=6xT /note="ApEinfo_fwdcolor=pink vntifkey=25 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" polyA_site complement(3008..3051) /label=minimal polyA /note="ApEinfo_fwdcolor=pink vntifkey=26 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" CDS complement(3058..3792) /codon_start=1 /label=gfp /note="ApEinfo_fwdcolor=pink vntifkey=4 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" /translation="TGRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLR QADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRLRPGAHHL LQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAE ERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRVAARQPLPEHPVRPEQRPQREARS HGPAGVRDRRRDHSRHGRAVQVK" promoter complement(3814..4435) /label=RSV /note="ApEinfo_fwdcolor=pink vntifkey=29 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" misc_feature complement(4454..4594) /label=AAV-2 ITR /note="ApEinfo_fwdcolor=pink vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" primer_bind complement(4637..4659) /label=T7 Primer /note="ApEinfo_fwdcolor=pink vntifkey=28 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtatgcatc 2221 ctgcaggacg cgtcctaccc actccctcta tgcgcgctcg ctcactcact cggccctgga 2281 gaccaaaggt ctccagactg ccggcctctg gccggcaggg ccgagtgagt gagcgagcgc 2341 gcatagaggg agtgggtacc tccatcatct aggtttgcca gatctgatat cggcgcgccc 2401 gagtccaaca cccgtgggaa tcccatgggc accatggccc ctcgctccaa aaatgctttc 2461 gcgtcgcgca gacactgctc ggtagtttcg gggatcagcg tttgagtaag agcccgcgtc 2521 tgaaccctcc gcgccgcccc ggccccagtg gaaagacgcg caggcaaaac gcaccacgtg 2581 acggagcgtg accgcgcgcc gagcgcgcgc caaggtcggg caggaagagg gcctatttcc 2641 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa 2701 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct 2761 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac 2821 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acacccatca 2881 agtcagccat cagcgtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct 2941 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgcg gccgcctcga 3001 gtgatcaaaa aaccaacaca cgcttccaat gaaaataaac gatcctttat tgctagcctt 3061 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg 3121 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag 3181 tggttgtcgg gcagcaacac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg 3241 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg 3301 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg 3361 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc 3421 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag 3481 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc 3541 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg 3601 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg 3661 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg 3721 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg 3781 gtggcgaccg gtggatcccg ggcccgcggt acccagcttg gaggtgcaca ccaatgtggt 3841 gaatggtcaa atggcgttta ttgtatcgag ctaggcactt aaatacaata tctctgcaat 3901 gcggaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc ctaataaggc 3961 acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct ctccttgtaa 4021 ggcatgttgc taactcatcc ttaccatgtt gcaagactac caagagtatt tgcataagac 4081 tacatttccc cctccctatg caaaagcgaa actactatat cctgagggga ctcctaaccg 4141 cgtacaaccg aagccccgct tttcgcctaa acacacccta gtcccctcag atacgcgtat 4201 atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc 4261 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga 4321 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg 4381 tactgagagt gcaccatacg gatctgcgat gataagctgt caaacatgag aattggtcga 4441 cgatatcaga tctaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 4501 tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc 4561 tcagtgagcg agcgagcgcg cagagaggga gtggcccccc cccccccccc cccctgcatt 4621 ctagagagct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 4681 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 4741 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 4801 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 4861 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 4921 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 4981 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 5041 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 5101 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 5161 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 5221 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 5281 tag //