LOCUS Exported 2839 bp ds-DNA circular SYN 22-JUN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2839) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 22, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2839 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 16..33 /locus_tag="operator O2" /label=operator O2 promoter complement(138..166) /locus_tag="araC promoter" /label=araC promoter protein_bind 174..195 /locus_tag="operator O1" /label=operator O1 misc_binding 217..230 /locus_tag="CAP site" /label=CAP site protein_bind 226..264 /locus_tag="Operator I2 and I1" /label=Operator I2 and I1 promoter 263..290 /locus_tag="pBAD promoter" /label=pBAD promoter misc_feature 297..316 /locus_tag="W108" /label=W108 misc_feature 317..358 /locus_tag="JAD designed chimera RNA 3'UTR" /label=JAD designed chimera RNA 3'UTR misc_feature 363..376 /locus_tag="Streptococcus pyogenes tracrRNA terminator" /label=Streptococcus pyogenes tracrRNA terminator /label=Streptococcus pyogenes tracrRNA terminator misc_feature 377..401 /locus_tag="1Xms2" /label=1Xms2 misc_feature 402..408 /locus_tag="SUP4 terminator" /label=SUP4 terminator terminator 409..776 /locus_tag="TrrnB" /label=TrrnB terminator 702..769 /locus_tag="sglTerm" /label=sglTerm rep_origin complement(962..1644) /direction=LEFT /locus_tag="ColE1 origin" /label=ColE1 origin CDS complement(1742..2401) /locus_tag="AmpR" /label=AmpR misc_feature complement(2641..2669) /locus_tag="AmpR promoter" /label=AmpR promoter ORIGIN 1 gaattctaaa gatctgaaac caattgtcca tattgcatca gacattgccg tcactgcgtc 61 ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa 121 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga 181 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc 241 ataagattag cggattctac ctgacgcttt ttatcgcaac tctctactgt actagtgaag 301 atccggcctg cagccagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt 361 atcaacttga aaaagtgcgc acatgaggat cacccatgtg ctttttttga agcttgggcc 421 cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca 481 tcattgagtt taaacggtct ccagcttggc tgttttggcg gatgagagaa gattttcagc 541 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc 601 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 661 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg 721 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaactggat 781 ccttactcga gtctagactg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc 841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 1021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 1081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 1141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 1201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 1261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 1321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 1381 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 1441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 1501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 1561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 1621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 1681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1861 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 2041 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 2101 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 2161 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 2221 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 2281 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 2341 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 2401 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 2461 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 2521 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 2581 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 2641 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 2701 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 2761 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 2821 tcgctaagga tgatttctg //