LOCUS       Exported                2839 bp ds-DNA     circular SYN 22-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    16..33
                     /locus_tag="operator O2"
                     /label=operator O2
     promoter        complement(138..166)
                     /locus_tag="araC promoter"
                     /label=araC promoter
     protein_bind    174..195
                     /locus_tag="operator O1"
                     /label=operator O1
     misc_binding    217..230
                     /locus_tag="CAP site"
                     /label=CAP site
     protein_bind    226..264
                     /locus_tag="Operator I2 and I1"
                     /label=Operator I2 and I1
     promoter        263..290
                     /locus_tag="pBAD promoter"
                     /label=pBAD promoter
     misc_feature    297..316
                     /locus_tag="W108"
                     /label=W108
     misc_feature    317..358
                     /locus_tag="JAD designed chimera RNA 3'UTR"
                     /label=JAD designed chimera RNA 3'UTR
     misc_feature    363..376
                     /locus_tag="Streptococcus pyogenes tracrRNA terminator"
                     /label=Streptococcus pyogenes tracrRNA terminator
                     /label=Streptococcus pyogenes tracrRNA terminator
     misc_feature    377..401
                     /locus_tag="1Xms2"
                     /label=1Xms2
     misc_feature    402..408
                     /locus_tag="SUP4 terminator"
                     /label=SUP4 terminator
     terminator      409..776
                     /locus_tag="TrrnB"
                     /label=TrrnB
     terminator      702..769
                     /locus_tag="sglTerm"
                     /label=sglTerm
     rep_origin      complement(962..1644)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(1742..2401)
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_feature    complement(2641..2669)
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
ORIGIN
        1 gaattctaaa gatctgaaac caattgtcca tattgcatca gacattgccg tcactgcgtc
       61 ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa
      121 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga
      181 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc
      241 ataagattag cggattctac ctgacgcttt ttatcgcaac tctctactgt actagtgaag
      301 atccggcctg cagccagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      361 atcaacttga aaaagtgcgc acatgaggat cacccatgtg ctttttttga agcttgggcc
      421 cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca
      481 tcattgagtt taaacggtct ccagcttggc tgttttggcg gatgagagaa gattttcagc
      541 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
      601 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
      661 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
      721 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaactggat
      781 ccttactcga gtctagactg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1381 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1861 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2041 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2101 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2161 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2221 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2281 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2341 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2401 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2461 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2521 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2581 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2641 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2701 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     2761 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     2821 tcgctaagga tgatttctg
//
