LOCUS       Exported                3263 bp ds-DNA     circular SYN 22-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(861..889)
                     /label=AmpR promoter
     misc_feature    1075..1701
                     /label=TetR
     misc_feature    1702..1774
                     /label=tet promoter region
     misc_feature    1723..1740
                     /label=Q1, Q2 promoter (tetR)
     misc_feature    1753..1771
                     /label=TetO2 site
     misc_feature    1781..1800
                     /label=W108
     misc_feature    1801..1842
                     /label=JAD designed chimera RNA 3'UTR
     misc_feature    1847..1861
                     /label=Streptococcus pyogenes tracrRNA terminator
     misc_feature    1848..1861
                     /label=Hairpin 3
     misc_feature    1862..1884
                     /label=MS2 with 2bp stem extension
     misc_feature    1862..1863
                     /label=Left stem extension
     misc_feature    1883..1884
                     /label=Right stem extension
     misc_feature    1885..1891
                     /label=SUP4 terminator
     terminator      1892..2259
                     /label=TrrnB
     terminator      2185..2252
                     /label=sglTerm
     rep_origin      complement(2445..3127)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(join(3225..3263,1..621))
                     /label=AmpR
ORIGIN
        1 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
       61 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
      421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
      481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
      541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
      601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
      661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
      721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
      781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
      961 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     1021 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattctaaag atctttaaga
     1081 cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa ggccgaataa
     1141 gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta ataatggcgg
     1201 catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct cttgatcttc
     1261 caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg cattctctag
     1321 tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat actgtttttc
     1381 tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta aagcacatct
     1441 aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa
     1501 gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag cccgcttatt
     1561 ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga gtttacgggt
     1621 tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa tcactttact
     1681 tttatctaat ctagacatca ttaattccta atttttgttg acactctatc gttgatagag
     1741 ttattttacc actccctatc agtgatagag aaaaactagt gaagatccgg cctgcagcca
     1801 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     1861 ggcacatgag gatcacccat gtgctttttt tgaagcttgg gcccgaacaa aaactcatct
     1921 cagaagagga tctgaatagc gccgtcgacc atcatcatca tcatcattga gtttaaacgg
     1981 tctccagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
     2041 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     2101 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     2161 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     2221 actgggcctt tcgttttatc tgttgtttgt cggtgaactg gatccttact cgagtctaga
     2281 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2341 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2401 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2461 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2521 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2581 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2641 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2701 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2761 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2821 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2881 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2941 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3001 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3061 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3121 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3181 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3241 gaggcaccta tctcagcgat ctg
//
