LOCUS       Exported               14027 bp ds-DNA    circular SYN 16-DEC-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pPB-dCas9-p300-T2A-MpH
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 14027)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported 01 Aug 2018 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14027
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    592..616
                     /note="oligo: M13F"
     misc_feature    670..983
                     /note="Left repeat (prom)"
     misc_feature    993..1005
                     /note="HindIIIXhoI-EM7neo"
                     /note="Entered into Gene Construction Kit by Keith Johnson,
                     9/4/91.
                     ID   ARBL2KSP   standard; DNA; SYN; 2961 BP.
                     XX
                     AC   X52327;
                     XX
                     DT   10-MAY-1990 (Rel. 24; Last updated; Version 1)
                     DT   10-MAY-1990 (Rel. 24; Created)
                     XX
                     DE   pBluescript II KS(+) vector DNA, phagemid excised from
                     lambda ZAPII
                     XX
                     KW   artificial sequence; cloning vector; expression 
                     vector; vector.
                     XX
                     OS   Cloning vector
                     OC   Artificial sequences; Cloning vectors.
                     XX
                     RN   [1]
                     RB   1-2961
                     RA   Thomas E.A.;
                     RT   ;
                     RL   Submitted (20-FEB-1990) on tape to the EMBL Data 
                     Library by:
                     RL   Thomas E.A., Stratagene Clonin Systems, 11099 North 
                     Torney Pines
                     RL   Rd., La Jolla, CA 92037, USA.
                     XX
                     RN   [2]
                     RA   Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
                     RT   ""Lambda ZAP:A bacteriophage lambda expression vector 
                     with in vivo
                     RT   excision properties"";
                     RL   Nucleic Acids Res. 16:7583-7600(1988).
                     XX
                     RN   [3]
                     RA   Alting-Mees M.A., Short J.M.;
                     RT   ""pBluescript II: gene mapping vectors."";
                     RL   Nucleic Acids Res. 17:9494-9494(1989).
                     XX
                     FH   Key             Location/Qualifiers
                     FH
                     FT   misc_feature    1. .2961
                     FT                   /note=""phagemid pBluescriptII KS(+)""
                     XX
                     SQ   Sequence  2961 BP;  747 A; 738 C; 755 G; 721 T; 0 
                     other;
                     Arbl2ksp  Length: 2961  August 22, 1991  11:07  Type: N  
                     Check: 7739  .."
     protein_bind    998..1022
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    998..1005
                     /note="attR1"
     misc_feature    1006..1022
                     /note="attL1"
     misc_feature    1060..2659
                     /note="EF1a-1.6"
     misc_feature    1060..2659
                     /note="pPB-EF1a1.8-GFP"
     misc_feature    2669..2675
                     /note="3. pENTR-EF1a-GFP_AscRI-IRESneo"
     misc_feature    2685..2699
                     /note="Gibson arm for VP64 R primer"
     CDS             2687..6916
                     /codon_start=1
                     /note="dCas9-VP64-T2Ablast"
                     /translation="MKRPAATKKAGQAKKKKDKKYSIGLAIGTNSVGWAVITDEYKVPS
                     KKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN
                     EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD
                     KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGV
                     DAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQ
                     LSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKR
                     YDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
                     DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN
                     LPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVT
                     VKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDI
                     VLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKT
                     ILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGI
                     LQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQIL
                     KEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNK
                     VLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
                     TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQ
                     VNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVE
                     KGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENG
                     RKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDE
                     IIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYF
                     DTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSAGGGGSGGGGSGGGGSG
                     PKKKRKVA"
     misc_feature    2690..2737
                     /note="NLS"
                     /note="     CDS             3881..3928
                                          /codon_start=1
                                          /product=""bipartite nuclear 
                     localization signal from
                                          nucleoplasmin""
                                          /note=""nucleoplasmin NLS""
                                          /translation=""KRPAATKKAGQAKKKK""
                     "
     CDS             2690..2737
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6893..6913
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    6893..6913
                     /note="NLS"
                     /note="     CDS             8084..8104
                                          /codon_start=1
                                          /product=""nuclear localization signal
                     of SV40 large T
                                          antigen""
                                          /note=""SV40 NLS""
                                          /translation=""PKKKRKV""
                     "
     misc_feature    6902..6916
                     /note="gibson assembly arm"
     misc_feature    6917..6934
                     /note="Linker seq"
     CDS             6935..8785
                     /codon_start=1
                     /note="p300 core"
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     misc_feature    7433..7448
                     /note="Gibson homology arm"
     CDS             8786..8839
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8849..10264
                     /codon_start=1
                     /note="MS2p65HSF1"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIYSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSPSGQ
                     ISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAGE
                     GTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEP
                     MLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS
                     SGQGGGGSGFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPRPPEAEN
                     SSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAEDPTISLL
                     TGSEPPKAKDPTVS"
     misc_feature    8849..8867
                     /note="R homology arm for MS2 fusion"
     misc_feature    8849..8867
                     /note="Gibson homology arm"
     CDS             9290..9310
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    9290..9310
                     /note="NLS"
                     /note="     CDS             4322..4342
                                          /codon_start=1
                                          /product=""nuclear localization signal
                     of SV40 large T
                                          antigen""
                                          /note=""SV40 NLS""
                                          /translation=""PKKKRKV""
                     "
     misc_feature    10258..10274
                     /note="L homology arm for MS2 fusion"
     misc_feature    10258..10274
                     /note="Gibson homology arm"
     misc_feature    10276..11500
                     /note="0. pENTR-EF1a-dCas9VP6-T2A-MS2p65HSF1-IRESneo"
     misc_feature    10281..10863
                     /note="IRES"
     misc_feature    10281..10863
                     /note="1. BsdbpA"
     misc_feature    10309..10328
                     /note="IRESbsdbpA-seq2"
     misc_feature    complement(10409..10428)
                     /note="IRESbsdbpA-seq8"
     misc_feature    10509..10528
                     /note="IRESbsdbpA-seq3"
     misc_feature    10709..10728
                     /note="IRESbsdbpA-seq4"
     misc_feature    complement(10856..10885)
                     /note="IRES-L1"
     misc_feature    10861..10895
                     /note="BsdbpA-U1"
     misc_feature    10869..10875
                     /note="pKLV2-EF1aBsdCas9fz [4581-4976]"
     CDS             10876..11271
                     /codon_start=1
                     /note="Bsd"
                     /note="Sequence from pMXs-GFP2ABsd
                     "
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10876..11271
                     /note="Bad2Acas9FZ"
     misc_feature    10909..10928
                     /note="IRESbsdbpA-seq5"
     misc_feature    11109..11128
                     /note="IRESbsdbpA-seq6"
     misc_feature    complement(11251..11280)
                     /note="BsdbpA-L1"
     misc_feature    11256..11290
                     /note="BsdbpA-U2"
     misc_feature    11272..11499
                     /note="bpA"
     misc_feature    11272..11499
                     /note="pKLV2-EF1aBsdCas9fz [4581-4976]"
     misc_feature    11309..11328
                     /note="IRESbsdbpA-seq7"
     protein_bind    complement(11520..11544)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    11520..11529
                     /note="attL2"
     misc_feature    11530..11544
                     /note="attR2"
     misc_feature    11551..11792
                     /note="right repeat (pA)"
     misc_feature    complement(11868..11897)
                     /note="oligo: M13R"
     CDS             complement(13042..13899)
                     /codon_start=1
                     /note="amp"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcaca agtttgtaca aaaaagcagg
     1021 ctggcgccgg aaccaattca gtcgactgga tccacgcgtg aggggcgcgg aagccggggc
     1081 ggaggaggag ccaaccccgg gctgggctga gacccgcaga ggaagacgct ctagggattt
     1141 gtcccggact agcgagatgg caaggctgag gacgggaggc tgattgagag gcgaaggtac
     1201 accctaatct caatacaacc tttggagcta agccagcaat ggtagaggga agattctgca
     1261 cgtcccttcc aggcggcctc cccgtcacca ccccccccaa cccgccccga ccggagctga
     1321 gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa
     1381 ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc
     1441 gtcatcactg aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc
     1501 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     1561 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     1621 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     1681 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     1741 ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct gcagtacgtg
     1801 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     1861 ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg ccgccgcgtg
     1921 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     1981 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     2041 caagatcgat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2101 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2161 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2221 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     2281 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     2341 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     2401 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     2461 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     2521 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     2581 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     2641 tccatttcag gtgtcgtggg aagcttggcg cgcccgtacg gccaccatga aaaggccggc
     2701 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac aagaagtaca gcatcggcct
     2761 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     2821 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     2881 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
     2941 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     3001 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     3061 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     3121 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     3181 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     3241 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     3301 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     3361 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     3421 gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgggcctgac
     3481 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
     3541 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
     3601 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
     3661 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
     3721 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
     3781 gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca
     3841 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     3901 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     3961 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     4021 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     4081 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     4141 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca
     4201 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     4261 caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata
     4321 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt
     4381 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     4441 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     4501 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     4561 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     4621 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     4681 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     4741 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     4801 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     4861 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     4921 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     4981 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     5041 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     5101 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     5161 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     5221 catcaaccgg ctgtccgact acgatgtgga ccacatcgtg cctcagagct ttctgaagga
     5281 cgactccatc gacaacaagg tgctgaccag aagcgacaag gcccggggca agagcgacaa
     5341 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc
     5401 caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag
     5461 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     5521 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     5581 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     5641 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     5701 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     5761 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     5821 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     5881 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     5941 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     6001 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     6061 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     6121 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     6181 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     6241 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     6301 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     6361 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     6421 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     6481 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     6541 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     6601 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     6661 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     6721 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     6781 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacag
     6841 cgctggagga ggtggaagcg gaggaggagg aagcggagga ggaggtagcg gacctaagaa
     6901 aaagaggaag gtggcgggag gtggaagcgg aggaattttc aaaccagaag aactacgaca
     6961 ggcactgatg ccaactttgg aggcacttta ccgtcaggat ccagaatccc ttccctttcg
     7021 tcaacctgtg gaccctcagc ttttaggaat ccctgattac tttgatattg tgaagagccc
     7081 catggatctt tctaccatta agaggaagtt agacactgga cagtatcagg agccctggca
     7141 gtatgtcgat gatatttggc ttatgttcaa taatgcctgg ttatataacc ggaaaacatc
     7201 acgggtatac aaatactgct ccaagctctc tgaggtcttt gaacaagaaa ttgacccagt
     7261 gatgcaaagc cttggatact gttgtggcag aaagttggag ttctctccac agacactgtg
     7321 ttgctacggc aaacagttgt gcacaatacc tcgtgatgcc acttattaca gttaccagaa
     7381 caggtatcat ttctgtgaga agtgtttcaa tgagatccaa ggggagagcg tttctttggg
     7441 ggatgaccct tcccagcctc aaactacaat aaataaagaa caattttcca agagaaaaaa
     7501 tgacacactg gatcctgaac tgtttgttga atgtacagag tgcggaagaa agatgcatca
     7561 gatctgtgtc cttcaccatg agatcatctg gcctgctgga ttcgtctgtg atggctgttt
     7621 aaagaaaagt gcacgaacta ggaaagaaaa taagttttct gctaaaaggt tgccatctac
     7681 cagacttggc acctttcttg agaatcgtgt gaatgacttt ctgaggcgac agaatcaccc
     7741 tgagtcagga gaggtcactg ttagagtagt tcatgcttct gacaaaaccg tggaagtaaa
     7801 accaggcatg aaagcaaggt ttgtggacag tggagagatg gcagaatcct ttccataccg
     7861 aaccaaagcc ctctttgcct ttgaagaaat tgatggtgtt gacctgtgct tctttggcat
     7921 gcatgttcaa gagtatggct ctgactgccc tccacccaac cagaggagag tatacatatc
     7981 ttacctcgat agtgttcatt tcttccgtcc taaatgcttg aggactgcag tctatcatga
     8041 aatcctaatt ggatatttag aatatgtcaa gaaattaggt tacacaacag ggcatatttg
     8101 ggcatgtcca ccaagtgagg gagatgatta tatcttccat tgccatcctc ctgaccagaa
     8161 gatacccaag cccaagcgac tgcaggaatg gtacaaaaaa atgcttgaca aggctgtatc
     8221 agagcgtatt gtccatgact acaaggatat ttttaaacaa gctactgaag atagattaac
     8281 aagtgcaaag gaattgcctt atttcgaggg tgatttctgg cccaatgttc tggaagaaag
     8341 cattaaggaa ctggaacagg aggaagaaga gagaaaacga gaggaaaaca ccagcaatga
     8401 aagcacagat gtgaccaagg gagacagcaa aaatgctaaa aagaagaata ataagaaaac
     8461 cagcaaaaat aagagcagcc tgagtagggg caacaagaag aaacccggga tgcccaatgt
     8521 atctaacgac ctctcacaga aactatatgc caccatggag aagcataaag aggtcttctt
     8581 tgtgatccgc ctcattgctg gccctgctgc caactccctg cctcccattg ttgatcctga
     8641 tcctctcatc ccctgcgatc tgatggatgg tcgggatgcg tttctcacgc tggcaaggga
     8701 caagcacctg gagttctctt cactccgaag agcccagtgg tccaccatgt gcatgctggt
     8761 ggagctgcac acgcagagcc aggacgaggg cagaggaagt ctcctaacat gcggtgacgt
     8821 ggaggagaat cctggcccag gaggtatggc ttcaaacttt actcagttcg tgctcgtgga
     8881 caatggtggg acaggggatg tgacagtggc tccttctaat ttcgctaatg gggtggcaga
     8941 gtggatcagc tccaactcac ggagccaggc ctacaaggtg acatgcagcg tcaggcagtc
     9001 tagtgcccag aagagaaagt ataccatcaa ggtggaggtc cccaaagtgg ctacccagac
     9061 agtgggcgga gtcgaactgc ctgtcgccgc ttggaggtcc tacctgaaca tggagctcac
     9121 tatcccaatt ttcgctacca attctgactg tgaactcatc gtgaaggcaa tgcaggggct
     9181 cctcaaagac ggtaatccta tcccttccgc catcgccgct aactcaggta tctacagcgc
     9241 tggaggaggt ggaagcggag gaggaggaag cggaggagga ggtagcggac ctaagaaaaa
     9301 gaggaaggtg gcggccgctg gatccccttc agggcagatc agcaaccagg ccctggctct
     9361 ggcccctagc tccgctccag tgctggccca gactatggtg ccctctagtg ctatggtgcc
     9421 tctggcccag ccacctgctc cagcccctgt gctgacccca ggaccacccc agtcactgag
     9481 cgctccagtg cccaagtcta cacaggccgg cgaggggact ctgagtgaag ctctgctgca
     9541 cctgcagttc gacgctgatg aggacctggg agctctgctg gggaacagca ccgatcccgg
     9601 agtgttcaca gatctggcct ccgtggacaa ctctgagttt cagcagctgc tgaatcaggg
     9661 cgtgtccatg tctcatagta cagccgaacc aatgctgatg gagtaccccg aagccattac
     9721 ccggctggtg accggcagcc agcggccccc cgaccccgct ccaactcccc tgggaaccag
     9781 cggcctgcct aatgggctgt ccggagatga agacttctca agcatcgctg atatggactt
     9841 tagtgccctg ctgtcacaga tttcctctag tgggcaggga ggaggtggaa gcggcttcag
     9901 cgtggacacc agtgccctgc tggacctgtt cagcccctcg gtgaccgtgc ccgacatgag
     9961 cctgcctgac cttgacagca gcctggccag tatccaagag ctcctgtctc cccaggagcc
    10021 ccccaggcct cccgaggcag agaacagcag cccggattca gggaagcagc tggtgcacta
    10081 cacagcgcag ccgctgttcc tgctggaccc cggctccgtg gacaccggga gcaacgacct
    10141 gccggtgctg tttgagctgg gagagggctc ctacttctcc gaaggggacg gcttcgccga
    10201 ggaccccacc atctccctgc tgacaggctc ggagcctccc aaagccaagg accccactgt
    10261 ctcctgtaca tgaggaattc cgcccccccc ccctctccct cccccccccc taacgttact
    10321 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
    10381 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
    10441 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
    10501 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
    10561 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
    10621 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
    10681 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
    10741 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
    10801 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
    10861 aatatggcca caaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
    10921 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
    10981 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
    11041 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
    11101 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
    11161 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
    11221 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ccgactgtgc
    11281 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
    11341 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
    11401 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
    11461 tcaatagcag gcatgctggg gatgcggtgg gctctatgga ctagtctcga gatatctaga
    11521 cccagctttc ttgtacaaag tggtactagt taaaagtttt gttactttat agaagaaatt
    11581 ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta
    11641 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
    11701 cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt
    11761 acgtcacaat atgattatct ttctagggtt aatctagtat acgcgtatgc ggccgcttaa
    11821 ttaatccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
    11881 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    11941 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    12001 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    12061 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    12121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    12181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    12241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    12301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    12361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    12421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    12481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    12541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    12601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    12661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    12721 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    12781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    12841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    12901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    12961 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    13021 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    13081 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    13141 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    13201 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    13261 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    13321 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    13381 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    13441 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    13501 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    13561 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    13621 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    13681 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    13741 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    13801 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    13861 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    13921 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    13981 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
