LOCUS       Exported                1714 bp ds-DNA     linear   UNA 23-JUN-2018
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   æaa¼º
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     1..17
                     /label=OZJ093
     primer_bind     1..17
                     /label=PupE.FOR
     misc_feature    1..3
                     /label=ATG
     CDS             4..255
                     /codon_start=1
                     /label=biotin tag
                     /translation="AGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETE
                     INAPTDGKVEKVLVKERDAVQGGQGLIKIGDYDIPTTAS"
     primer_bind     4..17
                     /label=XY1605.FOR
     primer_bind     complement(236..265)
                     /label=XY1606.REV
     primer_bind     246..276
                     /label=XY1607.FOR
     CDS             256..447
                     /codon_start=1
                     /label=PupE
                     /translation="MNAKQTQIMGGGGRDEDNAEDSAQASGQVQINTEGVDSLLDEIDG
                     LLENNAEEFVRSYVQKGGE"
     primer_bind     complement(421..451)
                     /label=XY1608.REV
     primer_bind     complement(422..469)
                     /label=PupE.REV
     primer_bind     451..479
                     /label=ires2n.FOR
     primer_bind     451..466
                     /label=IRES 1.FOR
     misc_feature    452..1013
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(984..1038)
                     /label=IRES 1.REV
     primer_bind     996..1036
                     /label=BFP 2.FOR
     misc_feature    1016..1018
                     /label=Start
     CDS             1019..1714
                     /codon_start=1
                     /label=BFP
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    1690
                     /label=NewT>G mutation does not change aa residue
                     /label=NewT>G mutation does not change aa residue (stays p
     primer_bind     complement(1693..1714)
                     /label=BFP 2.REV
     primer_bind     complement(1694..1714)
                     /label=OZJO94
ORIGIN
        1 atggccggga aggcaggcga gggagagatc cccgcaccct tggccggcac ggtcagcaaa
       61 atcctggtca aggaaggcga caccgtgaag gctggacaga cggtgttggt actggaggcg
      121 atgaagatgg agacagagat caatgccccg accgatggga aggtggagaa ggtgttggtt
      181 aaggaaaggg acgccgtgca gggcggtcag ggactgatca agatcggcga ctacgacatc
      241 ccgacaaccg ccagcatgaa cgcgaaacag acccagatca tgggtggcgg tggtcgtgac
      301 gaagacaatg cggaagactc tgctcaggcg tctggtcagg ttcagatcaa taccgaaggt
      361 gttgactctc tgctggacga aatcgacggc ctgctcgaaa acaacgcgga ggaattcgtt
      421 cgttcttacg ttcagaaagg tggtgaataa gccccccccc ctaacgttac tggccgaagc
      481 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
      541 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      601 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      661 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      721 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
      781 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
      841 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
      901 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
      961 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgag
     1021 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
     1081 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     1141 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
     1201 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
     1261 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     1321 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     1381 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     1441 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
     1501 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
     1561 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
     1621 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
     1681 cgacctccct agcaaactgg ggcacaagct taat
//
