LOCUS Exported 9409 bp ds-DNA circular SYN 26-JUN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9409) AUTHORS Lijie Wang TITLE Direct Submission JOURNAL Exported Jun 26, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9409 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 132..335 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 377..395 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 409..1005 /codon_start=1 /label=A3A /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW GCAGEVRAFLQENTHVRLRIFAARIYDDDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN" misc_feature 802..804 /label=Y132D CDS 1006..1053 /codon_start=1 /label=XTEN linker /translation="SGSETPGTSESATPES" CDS 1054..5154 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9n /note="generates RNA-guided single strand nicks in DNA due to the D10 mutation in the RuvC-like catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5167..5415 /codon_start=1 /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 5428..5448 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5455..5508 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5509..5757 /codon_start=1 /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 5758..5778 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5779..5835 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 5836..6084 /codon_start=1 /label=UGI-2 /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 6085..6105 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6106..6159 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A-2 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6160..6408 /codon_start=1 /label=UGI-3 /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 6421..6441 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS-2 /translation="PKKKRKV" misc_feature 6464..6481 /label=BGH_rev_primer polyA_signal 6470..6694 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6765..6781) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 6789..6805 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6813..6843) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6858..6879 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7167..7755) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7926..8786) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8787..8891) /gene="bla" /label=AmpR promoter enhancer join(9161..9409,1..131) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggaagccagc 421 ccagcatccg ggcccagaca cttgatggat ccacacatat tcacttccaa ctttaacaat 481 ggcattggaa ggcataagac ctacctgtgc tacgaagtgg agcgcctgga caatggcacc 541 tcggtcaaga tggaccagca caggggcttt ctacacaacc aggctaagaa tcttctctgt 601 ggcttttacg gccgccatgc ggagctgcgc ttcttggacc tggttccttc tttgcagttg 661 gacccggccc agatctacag ggtcacttgg ttcatctcct ggagcccctg cttctcctgg 721 ggctgtgccg gggaagtgcg tgcgttcctt caggagaaca cacacgtgag actgcgtatc 781 ttcgctgccc gcatctatga tgatgacccc ctatataagg aggcactgca aatgctgcgg 841 gatgctgggg cccaagtctc catcatgacc tacgatgaat ttaagcactg ctgggacacc 901 tttgtggacc accagggatg tcccttccag ccctgggatg gactagatga gcacagccaa 961 gccctgagtg ggaggctgcg ggccattctc cagaatcagg gaaacagcgg cagcgagact 1021 cccgggacct cagagtccgc cacacccgaa agtgataaaa agtattctat tggtttagcc 1081 atcggcacta attccgttgg atgggctgtc ataaccgatg aatacaaagt accttcaaag 1141 aaatttaagg tgttggggaa cacagaccgt cattcgatta aaaagaatct tatcggtgcc 1201 ctcctattcg atagtggcga aacggcagag gcgactcgcc tgaaacgaac cgctcggaga 1261 aggtatacac gtcgcaagaa ccgaatatgt tacttacaag aaatttttag caatgagatg 1321 gccaaagttg acgattcttt ctttcaccgt ttggaagagt ccttccttgt cgaagaggac 1381 aagaaacatg aacggcaccc catctttgga aacatagtag atgaggtggc atatcatgaa 1441 aagtacccaa cgatttatca cctcagaaaa aagctagttg actcaactga taaagcggac 1501 ctgaggttaa tctacttggc tcttgcccat atgataaagt tccgtgggca ctttctcatt 1561 gagggtgatc taaatccgga caactcggat gtcgacaaac tgttcatcca gttagtacaa 1621 acctataatc agttgtttga agagaaccct ataaatgcaa gtggcgtgga tgcgaaggct 1681 attcttagcg cccgcctctc taaatcccga cggctagaaa acctgatcgc acaattaccc 1741 ggagagaaga aaaatgggtt gttcggtaac cttatagcgc tctcactagg cctgacacca 1801 aattttaagt cgaacttcga cttagctgaa gatgccaaat tgcagcttag taaggacacg 1861 tacgatgacg atctcgacaa tctactggca caaattggag atcagtatgc ggacttattt 1921 ttggctgcca aaaaccttag cgatgcaatc ctcctatctg acatactgag agttaatact 1981 gagattacca aggcgccgtt atccgcttca atgatcaaaa ggtacgatga acatcaccaa 2041 gacttgacac ttctcaaggc cctagtccgt cagcaactgc ctgagaaata taaggaaata 2101 ttctttgatc agtcgaaaaa cgggtacgca ggttatattg acggcggagc gagtcaagag 2161 gaattctaca agtttatcaa acccatatta gagaagatgg atgggacgga agagttgctt 2221 gtaaaactca atcgcgaaga tctactgcga aagcagcgga ctttcgacaa cggtagcatt 2281 ccacatcaaa tccacttagg cgaattgcat gctatactta gaaggcagga ggatttttat 2341 ccgttcctca aagacaatcg tgaaaagatt gagaaaatcc taacctttcg cataccttac 2401 tatgtgggac ccctggcccg agggaactct cggttcgcat ggatgacaag aaagtccgaa 2461 gaaacgatta ctccatggaa ttttgaggaa gttgtcgata aaggtgcgtc agctcaatcg 2521 ttcatcgaga ggatgaccaa ctttgacaag aatttaccga acgaaaaagt attgcctaag 2581 cacagtttac tttacgagta tttcacagtg tacaatgaac tcacgaaagt taagtatgtc 2641 actgagggca tgcgtaaacc cgcctttcta agcggagaac agaagaaagc aatagtagat 2701 ctgttattca agaccaaccg caaagtgaca gttaagcaat tgaaagagga ctactttaag 2761 aaaattgaat gcttcgattc tgtcgagatc tccggggtag aagatcgatt taatgcgtca 2821 cttggtacgt atcatgacct cctaaagata attaaagata aggacttcct ggataacgaa 2881 gagaatgaag atatcttaga agatatagtg ttgactctta ccctctttga agatcgggaa 2941 atgattgagg aaagactaaa aacatacgct cacctgttcg acgataaggt tatgaaacag 3001 ttaaagaggc gtcgctatac gggctgggga cgattgtcgc ggaaacttat caacgggata 3061 agagacaagc aaagtggtaa aactattctc gattttctaa agagcgacgg cttcgccaat 3121 aggaacttta tgcagctgat ccatgatgac tctttaacct tcaaagagga tatacaaaag 3181 gcacaggttt ccggacaagg ggactcattg cacgaacata ttgcgaatct tgctggttcg 3241 ccagccatca aaaagggcat actccagaca gtcaaagtag tggatgagct agttaaggtc 3301 atgggacgtc acaaaccgga aaacattgta atcgagatgg cacgcgaaaa tcaaacgact 3361 cagaaggggc aaaaaaacag tcgagagcgg atgaagagaa tagaagaggg tattaaagaa 3421 ctgggcagcc agatcttaaa ggagcatcct gtggaaaata cccaattgca gaacgagaaa 3481 ctttacctct attacctaca aaatggaagg gacatgtatg ttgatcagga actggacata 3541 aaccgtttat ctgattacga cgtcgatcac attgtacccc aatccttttt gaaggacgat 3601 tcaatcgaca ataaagtgct tacacgctcg gataagaacc gagggaaaag tgacaatgtt 3661 ccaagcgagg aagtcgtaaa gaaaatgaag aactattggc ggcagctcct aaatgcgaaa 3721 ctgataacgc aaagaaagtt cgataactta actaaagctg agaggggtgg cttgtctgaa 3781 cttgacaagg ccggatttat taaacgtcag ctcgtggaaa cccgccaaat cacaaagcat 3841 gttgcacaga tactagattc ccgaatgaat acgaaatacg acgagaacga taagctgatt 3901 cgggaagtca aagtaatcac tttaaagtca aaattggtgt cggacttcag aaaggatttt 3961 caattctata aagttaggga gataaataac taccaccatg cgcacgacgc ttatcttaat 4021 gccgtcgtag ggaccgcact cattaagaaa tacccgaagc tagaaagtga gtttgtgtat 4081 ggtgattaca aagtttatga cgtccgtaag atgatcgcga aaagcgaaca ggagataggc 4141 aaggctacag ccaaatactt cttttattct aacattatga atttctttaa gacggaaatc 4201 actctggcaa acggagagat acgcaaacga cctttaattg aaaccaatgg ggagacaggt 4261 gaaatcgtat gggataaggg ccgggacttc gcgacggtga gaaaagtttt gtccatgccc 4321 caagtcaaca tagtaaagaa aactgaggtg cagaccggag ggttttcaaa ggaatcgatt 4381 cttccaaaaa ggaatagtga taagctcatc gctcgtaaaa aggactggga cccgaaaaag 4441 tacggtggct tcgatagccc tacagttgcc tattctgtcc tagtagtggc aaaagttgag 4501 aagggaaaat ccaagaaact gaagtcagtc aaagaattat tggggataac gattatggag 4561 cgctcgtctt ttgaaaagaa ccccatcgac ttccttgagg cgaaaggtta caaggaagta 4621 aaaaaggatc tcataattaa actaccaaag tatagtctgt ttgagttaga aaatggccga 4681 aaacggatgt tggctagcgc cggagagctt caaaagggga acgaactcgc actaccgtct 4741 aaatacgtga atttcctgta tttagcgtcc cattacgaga agttgaaagg ttcacctgaa 4801 gataacgaac agaagcaact ttttgttgag cagcacaaac attatctcga cgaaatcata 4861 gagcaaattt cggaattcag taagagagtc atcctagctg atgccaatct ggacaaagta 4921 ttaagcgcat acaacaagca cagggataaa cccatacgtg agcaggcgga aaatattatc 4981 catttgttta ctcttaccaa cctcggcgct ccagccgcat tcaagtattt tgacacaacg 5041 atagatcgca aacgatacac ttctaccaag gaggtgctag acgcgacact gattcaccaa 5101 tccatcacgg gattatatga aactcggata gatttgtcac agcttggggg tgactctggt 5161 ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact ggttatccag 5221 gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa gccggaaagc 5281 gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat gcttctgact 5341 agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa cggtgagaac 5401 aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcgg gcccgagggc 5461 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaac taatctgtca 5521 gatattattg aaaaggagac cggtaagcaa ctggttatcc aggaatccat cctcatgctc 5581 ccagaggagg tggaagaagt cattgggaac aagccggaaa gcgatatact cgtgcacacc 5641 gcctacgacg agagcaccga cgagaatgtc atgcttctga ctagcgacgc ccctgaatac 5701 aagccttggg ctctggtcat acaggatagc aacggtgaga acaagattaa gatgctcccc 5761 aagaagaaga ggaaagtcgc tactaacttc agcctgctga agcaggctgg agacgtggag 5821 gagaaccctg gacctaccaa cctgtccgat atcattgaga aagagaccgg caaacagctg 5881 gtgatccagg agagcatcct gatgctgccc gaagaggtgg aggaagtgat cggcaacaag 5941 cccgagtccg acatcctggt gcacacagcc tatgatgaat ccaccgacga gaacgtgatg 6001 ctgctgacct ccgatgctcc cgagtataaa ccctgggcac tggtgatcca ggactctaat 6061 ggagagaaca agatcaagat gctgcccaag aagaagagga aagtcgaagg acggggctcc 6121 ctcctgacct gtggcgatgt ggaagagaac cccggcccca caaacctcag tgacattatc 6181 gagaaggaaa caggaaaaca gctcgtcatt caagaatcta ttcttatgtt gcctgaggaa 6241 gtcgaagagg ttattggcaa taaacctgaa tctgatattc ttgtccatac cgcttacgat 6301 gagtccacag atgaaaatgt tatgctgctc acatctgacg caccagagta caaaccatgg 6361 gcgctcgtta ttcaagattc caacggcgaa aacaaaatca aaatgctttc tggtggttct 6421 cctaaaaaaa aaagaaaggt gtgagtttaa acccgctgat cagcctcgac tgtgccttct 6481 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 6541 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 6601 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 6661 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 6721 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 6781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 6841 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 6901 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 6961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 7021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 7081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 7141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 7201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 7261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 7321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 7381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 7441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 7501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 7561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 7621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 7681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 7741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 7801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 7861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 7921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 7981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 8041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 8101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 8161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 8221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 8281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 8341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 8401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 8461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 8521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 8581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 8641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 8701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 8761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 8821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 8881 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga 8941 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc 9001 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa 9061 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt 9121 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt 9181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 9241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 9301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 9361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatc //