LOCUS       pR\I-Ab\alpha\BirA        4670 bp    DNA     circular     19-JAN-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|578408823|
COMMENT     VNTDBDATE|579522249|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pR I-Ab alpha BirA|
COMMENT     VNTAUTHORNAME|James Moon|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             2649..3506
                     /vntifkey="4"
                     /label=APr
                     /note="bla gene- Ap(r) determinant"
     rep_origin      4268..4268
                     /vntifkey="33"
                     /label=ORI
                     /note="RNaseH cleavage point"
     promoter        4628..4628
                     /vntifkey="30"
                     /label=P(LAC)
                     /note="lac promoter"
     promoter        2614..2614
                     /vntifkey="30"
                     /label=P(BLA)
                     /note="bla gene promoter"
     misc_feature    2046..4670
                     /vntifkey="21"
                     /label=pUC\18
                     /note="sequence from pUC 18"
     CDS             2046..2300
                     /vntifkey="4"
                     /label=ALPHA
                     /note="lacZ gene fragment coding for LacZ alpha peptide"
     mutation        3827..3827
                     /vntifkey="62"
                     /label=mutation?
                     /note="C->T"
     polyA_signal    1296..2045
                     /vntifkey="25"
                     /label=ADH\poly\A
                     /note="polyadenylation signal from Drosophila ADH gene"
     promoter        10..376
                     /vntifkey="29"
                     /label=Metallothionein\Promoter
     misc_feature    376..376
                     /vntifkey="21"
                     /label=Transcription\Start\Site
     mutation        1664..1664
                     /vntifkey="62"
                     /label=Spe\mutation
                     /note="A->T point mutation of SpeI restriction site"
     CDS             511..1098
                     /vntifkey="4"
                     /label=I-Ab\alpha
     CDS             1099..1113
                     /vntifkey="4"
                     /label=Linker
     CDS             1114..1218
                     /vntifkey="4"
                     /label=Acidic\Leucine\Zipper
     sig_peptide     442..510
                     /vntifkey="94"
                     /label=Signal\Peptide
     CDS             1237..1281
                     /vntifkey="4"
                     /label=BirA
     CDS             1219..1236
                     /vntifkey="4"
                     /label=linker
BASE COUNT     1222 a      1144 c      1140 g      1164 t 
ORIGIN
        1 atgattaccg ttgcaggaca ggatgtggtg cccgatgtga ctagctcttt gctgcaggcc 
       61 gtcctatcct ctggttccga taagagaccc agaactccgg ccccccaccg cccaccgcca 
      121 cccccataca tatgtggtac gcaagtaaga gtgcctgcgc atgccccatg tgccccacca 
      181 agagctttgc atcccataca agtccccaaa gtggagaacc gaaccaattc ttcgcgggca 
      241 gaacaaaagc ttctgcacac gtctccactc gaatttggag ccggccggcg tgtgcaaaag 
      301 aggtgaatcg aacgaaagac ccgtgtgtaa agccgcgttt ccaaaatgta taaaaccgag 
      361 agcatctggc caatgtgcat cagttgtggt cagcagcaaa atcaagtgaa tcatctcagt 
      421 gcaactaaag gggaattcag gatgccgtgc agcagagctc tgattctggg ggtcctcgcc 
      481 ctgaccacca tgctcagcct ctgtggaggt gaagacgaca ttgaggccga ccacgtaggc 
      541 acctatggta taagtgtata tcagtctcct ggagacattg gccagtacac atttgaattt 
      601 gatggtgatg agttgttcta tgtggacttg gataagaagg agactgtctg gatgcttcct 
      661 gagtttggcc aattggcaag ctttgacccc caaggtggac tgcaaaacat agctgtagta 
      721 aaacacaact tgggagtctt gactaagagg tcaaattcca ccccagctac caatgaggct 
      781 cctcaagcga ctgtgttccc caagtcccct gtgctgctgg gtcagcccaa caccctcatc 
      841 tgctttgtgg acaacatctt ccctcctgtg atcaacatca catggctcag aaatagcaag 
      901 tcagtcgcag acggtgttta tgagaccagc ttcttcgtca accgtgacta ttccttccac 
      961 aagctgtctt atctcacctt catcccttct gacgatgaca tttatgactg caaggtggaa 
     1021 cactggggcc tggaggagcc ggttctgaaa cactgggaac ctgagattcc agcccccatg 
     1081 tcagagctga cagagactgg aggtggagga tccactacag ctccatcagc tcagctcgaa 
     1141 aaagagctcc aggccctgga gaaggaaaat gcacagctgg aatgggagtt gcaagcactg 
     1201 gaaaaggaac tggctcaggg cggcagcggc ggcagcggcc tgaacgatat ttttgaagcg 
     1261 cagaaaattg aatggcatga atgactcgac ctgcaggcat gcaagctatt cgatgcacac 
     1321 tcacattctt ctcctaatac gataataaaa ctttccatga aaaatatgga aaaatatatg 
     1381 aaaattgaga aatccaaaaa actgataaac gctctactta attaaaatag ataaatggga 
     1441 gcggcaggaa tggcggagca tggccaagtt cctccgccaa tcagtcgtaa aacagaagtc 
     1501 gtggaaagcg gatagaaaga atgttcgatt tgacgggcaa gcatgtctgc tatgtggcgg 
     1561 attgcggagg aattgcactg gagaccagca aggttctcat gaccaagaat atagcggtga 
     1621 gtgagcggga agctcggttt ctgtccagat cgaactcaaa acttgtccag ccagtcgctg 
     1681 tcgaaactaa ttaagttaat gagtttttca tgttagtttc gcgctgagca acaattaagt 
     1741 ttatgtttca gttcggctta gatttcgctg aaggacttgc cactttcaat caatacttta 
     1801 gaacaaaatc aaaactcatt ctaatagctt ggtgttcatc ttttttttta atgataagca 
     1861 ttttgtcgtt tatacttttt atatttcgat attaaaccac ctatgaagtc tattttaatc 
     1921 gccagataag caatatattg tgtaaatatt tgtattcttt atcaggaaat tcagggagac 
     1981 ggggaagtta ctatctacta aaagccaaac aatttcttac agttttactc tctctactct 
     2041 agagtagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 
     2101 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 
     2161 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc 
     2221 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 
     2281 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 
     2341 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
     2401 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 
     2461 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 
     2521 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     2581 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     2641 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     2701 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     2761 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     2821 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     2881 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     2941 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     3001 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     3061 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     3121 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     3181 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     3241 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     3301 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     3361 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     3421 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     3481 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     3541 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     3601 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     3661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     3721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     3781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 
     3841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     3901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     3961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     4021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     4081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     4141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     4201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     4261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     4321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     4381 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     4441 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
     4501 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
     4561 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
     4621 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 
//
