LOCUS Exported 5321 bp ds-DNA circular SYN 02-JUL-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5321) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 2, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5321 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(258..791) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(1123..1225) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin complement(1751..2296) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." ORIGIN 1 ctgagcaaac tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa 61 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac 121 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag 181 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct 241 gccactcatc gcagtactta ggtggcggta cttgggtcga tatcaaagtg catcacttct 301 tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc 361 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg 421 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca 481 ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct 541 tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg 601 gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca 661 agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc 721 atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg 781 ctgcgtaaca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat 841 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt 901 tccgaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa 961 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg 1021 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg 1081 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc 1141 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa 1201 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt 1261 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg 1321 tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat 1381 ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta 1441 ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat 1501 actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga 1561 aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg 1621 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg 1681 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg 1741 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa 1801 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct 1861 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc 1921 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg 1981 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt 2041 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga 2101 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt 2161 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac 2221 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag 2281 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt 2341 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt 2401 ctcatgtttg acagcattat catcgataag cttgatgcgt ggaagattga tcgtcttgca 2461 ccctgaaaag atgcaaaaat cttgctttaa tcgctggtac tcctgattct ggcactttat 2521 tctatgtctc tttcgcatct ggcgaaaagt cgtgtaccgg caaaggtgca gtcgttatat 2581 acttggagat tcatatggac gagttcgaga tgatcaaacg caacaccagc gaaatcatca 2641 gcgaagaaga actgcgcgaa gtgctgaaaa aagacgagaa gagcgcctat attggcttcg 2701 aaccgagcgg caaaattcat ctgggccact acctgcagat caagaagatg atcgatctgc 2761 agaacgcggg cttcgatatc atcattctgc tggccgatct gcatgcatac ctgaaccaga 2821 aaggcgaact ggacgaaatc cgcaaaatcg gcgactacaa caagaaagtg ttcgaagcga 2881 tgggcctgaa agcgaaatac gtctatggca gcgaatttca gctggacaaa gactacaccc 2941 tgaacgtgta tcgtctggca ctgaaaacca ccctgaaacg tgctcgtcgt tctatggaac 3001 tgattgcccg cgaagatgaa aacccgaaag tggcggaagt gatctatccg atcatgcagg 3061 tgaacgacat ccactatctg ggcgtagatg tagcagttgg tggcatggaa cagcgcaaaa 3121 tccacatgct ggctcgtgaa ctgctgccga aaaaagtcgt gtgcatccac aacccggttt 3181 taacgggtct ggatggtgaa ggcaaaatga gcagcagcaa aggcaacttt atcgccgtgg 3241 atgattctcc ggaagaaatc cgcgcgaaga tcaaaaaagc gtattgcccg gcaggtgtag 3301 ttgaaggtaa cccgatcatg gagatcgcga aatacttcct ggaatacccg ctgaccatta 3361 aacgcccgga aaaattcggt ggtgatctga ccgtgaacag ctacgaagaa ctggagagcc 3421 tgttcaagaa caaagaactg cacccgatgc gtctgaaaaa tgccgttgcg gaagagctga 3481 tcaaaatcct ggaaccgatt cgcaaacgtc tgtaagatct ggatcctcta cgccggacgc 3541 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc 3601 accgatgggg aagatcgggc tcgccacttc gggctcatga ggcatgcggc gccgcttctt 3661 tgagcgaacg atcaaaaata agtggcgccc catcaaaaaa atattctcaa cataaaaaac 3721 tttgtgtaat acttgtaacg ctgccatcag acgcattccg gcggtagttc agcagggcag 3781 aacggcggac tctaaatccg caggtcgctg gttcaaatcc ggcccgccgg accatttatc 3841 acagattgga aatttttgat ccttagcgaa agctaaggat tttttttagt cgaccgatgc 3901 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg 3961 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct 4021 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg 4081 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac 4141 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct 4201 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg 4261 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc 4321 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc 4381 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg 4441 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg 4501 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat 4561 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat 4621 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc 4681 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg 4741 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg 4801 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta 4861 aagtctggaa acgcggaagt cccctacgtg ctgctgaagt tgcccgcaac agagagtgga 4921 accaaccggt gataccacga tactatgact gagagtcaac gccatgagcg gcctcatttc 4981 ttattctgag ttacaacagt ccgcaccgct gtccggtagc tccttccggt gggcgcgggg 5041 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt 5101 gccggcagcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca 5161 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg 5221 aacacctaca tctgtattaa cgaagcgcta accgttttta tcaggctctg ggaggcagaa 5281 taaatgatca tatcgtcaat tattacctcc acggggagag c //