LOCUS       Exported                6596 bp ds-DNA     circular SYN 02-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             53..61
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             1136..1147
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             3847..3858
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4548..4605
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(4800..5388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      5571..5665
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5686..6345)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      6529..6572
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    6575..6596
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaatcaa attaaggagg
       61 taagataaat ggaacaggca atcattaatg atgaacggga gtatcttcgc catttctggc
      121 atcccgtatg tactgtaact gagcttgaaa aggcgcatcc ttccagcctc ggccccctgg
      181 ccgttaagct gctgaatgaa cagctcgttg tcgccaagct aggcgatgag tacgtcgcga
      241 tgcgtgatag atgcgctcat cgatcagcta agctttcctt gggtacagta agtggaaacc
      301 gcctacagtg cccctatcac ggatggcaat atgatacgca tggcgcttgc cagctcgtac
      361 cagcgtgccc caacagccca atacccaaca aggctaaagt tgatcgcttc gattgcgaag
      421 aacgctatgg attgatttgg attcgattgg actctagttt tgactgcact gaaattccct
      481 actttagtgc agccaacgat cctaggttgc gtattgttat acaagaacct tactggtggg
      541 atgcgactgc ggaacgtaga tgggaaaatt ttacagattt ttctcacttt gcattcattc
      601 acccaggcac gcttttcgat ccaaataatg ctgaacctcc aattgttccg atggatcgat
      661 tcaatggtca gtttcggttt gtctacgaca cgccagaaga tatggctgtc ccaaatcagg
      721 ctccaattgg ttcattttcg tatacttgca gcatgccgtt tgctattaac cttgaagtat
      781 ccaaatactc cagcagttcg ctgcatgtgt tattcaatgt gtcatgtccg gtagacagcc
      841 acaccacgaa aaactttctg atcttcgcta gggagcaatc ggacgactcg gattatctgc
      901 acattgcatt taatgatctc gtcttcgctg aagacaaacc agtaattgag tcccaatggc
      961 ctaaagacgc gccagcagat gaggtctcag tagtcgcaga taaggtatcg atacaatata
     1021 gaaaatggct gcgggaacta aaagaagctc ataaagaagg ttcacaagcc ttccgaagtg
     1081 ctttgttaga cccagtcatt gaaagcgata ggagctacat ataatactag agattaaaga
     1141 ggagaaatac tagatgtcta ctgaccaagt aatttttaac gactggcatc cagtcgctgc
     1201 tttggaagat gtatctctcg ataaacgata ccgctgtcga ctactaggtc gtacagttag
     1261 ttatgtgaaa acatctgatg ctgttaatgc tcattgggaa gaaagtgcag atgaaattaa
     1321 gaccatccgc gctaaagaaa tctatggtct tctgtggctc tcctttgccg acaaacccag
     1381 tgagatgttc gatattgcag agttcaagga acctgatcgc cgaatcgtca gcgctggatc
     1441 tgtgcgcgta aatgtctcag gactgcgtgc tatcgaaaac tttctggaca tggctcattt
     1501 cccttttgtt catacggata ttttgggtgc agagccactg acggaagttg agccgtataa
     1561 cgtcaactat gatgaaactg ttgatgagat cttcgctact gagtgtaagt tcccgcaacc
     1621 taaaggctca gctactgctg tcgagccaat tgatatgcag tatatatacc gcattacacg
     1681 gccatattcc gccattttat ataaaacttg cccgcccgaa ccgcatagat gggatgccct
     1741 cggtctgttt attcaacctg tagacgaaga ttggtgcata gcgcatacaa ttatgtgcta
     1801 tgtggatgac gttaactcag atcaacaact tcgccatttc cagcagacga tttttggcca
     1861 ggacttgatg attcttatca accaagttcc aaagcgtctt cccctggctg caagtcgaga
     1921 gagcccagtc cgggccgatg tgcttgcaac agcttatcgt cgctggctgc gtgagaaagg
     1981 tgtgcaatat ggtgctctgc gggactaata ctagagtcac acaggaaagt actagatgaa
     2041 caaacttgac gtcaaccagt ggtttcctat tgctaccact gaagatctcc cgaagcgcca
     2101 tgtctttcat gccacgttgt tggggcaaga aatggccatc tggcgcgatg actctggttc
     2161 agttaatgct tgggagaacc gctgcccgca tagaggattg cggttgacac tgggtgctaa
     2221 taccggtaac gagttgcggt gtcagtatca tggatggact tatgaaagcg ggactggtgg
     2281 ctgcactttt gtcccagccc atcgcgatgc accaccccca aatgccgcgc gggttaatac
     2341 ttttcctgtc cgcgaaaagc acggctttat ctggacgaca ttaggtcagc cgccaggaga
     2401 gcccatttca atcctcgatg acgctcagct tgtaaacgct gtaaaaacaa atctgcatag
     2461 cgtagttata gatgctgata ttgacggagt tgtcagcgtc ctacgtcaga atctttcagc
     2521 gttcatcgat gtgtttggtg cggccagcgc tgaagatctg catttgaaat ccatgctgca
     2581 agatcgaggg attctggtaa caagatcagg ctctattgct attcattttt atatgcagcg
     2641 ctcaaccatt agtaaatgcg ttgtacatgc gcaagtactt actccgggac gtccaggata
     2701 cgaacttcaa aagaactact cgtatgccat gaacgttatc cgcagggcag cagaagctgt
     2761 agctaccgac ttgattagca ttacagatat cagcgatcag actatcgaaa agcttgaagt
     2821 cgttagagaa aacatgacta aggctcctcc aacccactat atctgcgaag tggttacgcg
     2881 tactcaagag acaggtgata ttaactcata ctggctgaag cctatcggct acccactacc
     2941 agcattcagt ccagggatgc acatcagcat cacaacgccg gagggtagca ttcgacaata
     3001 ttccctcgtg aacgggcctg acgagcgtga atccttcatc atcggtgtga agaaagagat
     3061 tcagtcccgt ggcggctcca gatcaatgca cgaagatgtg aaggttggaa cgcaactaaa
     3121 agttacactt ccgaggaacg gttttccact cgtccaaacc agaaaacacc cgattctcgt
     3181 agcaggtggc atcggtatca ccccaatttt gtgtatggca caggctctgg atcagcaagg
     3241 ttcatcgtat gaaatacatt attttgctcg tgcatttgag catgttccat tccaggatcg
     3301 actgactgcg ttgggcgatc gtttgaatgt gcatcttggc ctcggcccag acgagactag
     3361 agcaaaactt gccgacatca tggagattca taacgcccaa gacgtagatg tttacacttg
     3421 cggcccgcaa ccaatgatcg aaactgtatc tgctgtcgct cttgctcatg gcatcgctga
     3481 agagtccatc cgatttgaat ttttcagtaa aaagaacgat gttcccgttt ctgatgaaga
     3541 atatgaggtt gagctcaaaa aaactggtca aatattcact gtctcgcctg gctctacgtt
     3601 gttgcaagct tgtttggaca acgatgttcg tatcgaagct tcttgtgagc agggtgtatg
     3661 cgggacttgt ataactccag tcgtatccgg cgatctcgag catcatgaca cttacctttc
     3721 taagaaagaa agggaaagcg gtaagtggat catgccgtgt gtttcgcgct gcaagtccaa
     3781 aaaaatcgtt ctcgatctgt gaggccgggc atcacccacg gtatggacga gctgtataaa
     3841 ctagagaaag aggagaaata ctagatgatc acgctttatg actatgaact ctcggggaat
     3901 tgctacaagg tgcggatgct cctttccatc ctgaagctac cgtacacgat agagacagtg
     3961 gaatttttcc cgtccaggga acataaatca ccggccttcc tgcgcatcaa tccactcggc
     4021 cagttgccgg tattgcgcga tgatgatttc gtgttgcgcg atgcccaggc catcctggtt
     4081 tacctggcga acaaatacga caccagtttg acctggtatc cgacacatgc gcccgccatc
     4141 ctcggccagg caaatatgtg gctcgcgttt gccgatggtt tgaccggctc gatttccgct
     4201 gcacgtttgc atgatttgtt tttttacgat ttcaatgcag agcaatgccg tgcccgcgcg
     4261 cacgaactac tgcgcatact ggacgaacac ctgtgggcgg cggaacaaaa tggacatcaa
     4321 tggctatgtc cggccgccgc acccaccatc gcggatcttg cctgcttccc gtatatcgca
     4381 ttgtcggatg aagccggcgt ttcactactc gactacccgg cggtacgacg ctggctggac
     4441 cgtgtcaaac gcatccccgg attcaccgtc atgtcgggga tttttccaac ctcagcttcc
     4501 ttcgagccgg cctcacttgc ggaagaaacc tgtcaataat actagagtcc ggcaaaaaag
     4561 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     4621 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4681 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4741 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4801 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4861 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4921 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4981 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5041 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5101 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5161 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5221 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5281 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5341 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5401 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5461 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5521 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     5581 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     5641 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     5701 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     5761 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     5821 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     5881 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     5941 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     6001 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     6061 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     6121 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     6181 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     6241 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     6301 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     6361 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     6421 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
     6481 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     6541 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagag
//
