LOCUS       pDA-12                  7332 bp    DNA     circular     28-MAR-2007
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|442062411|
COMMENT     VNTDBDATE|442062431|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDA-12|
COMMENT     VNTAUTHORNAME|Miguel Valvano|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(4184..5665)
                     /vntifkey="4"
                     /label=Mob
     CDS             6182..6853
                     /vntifkey="4"
                     /label=REP
     CDS             732..2003
                     /vntifkey="4"
                     /label=TetA
     CDS             complement(54..728)
                     /vntifkey="4"
                     /label=TetR
     promoter        2213..2602
                     /vntifkey="30"
                     /label=dhfr\prom\region
     -35_signal      2243..2248
                     /vntifkey="48"
                     /label=putative\-35
     -10_signal      2266..2271
                     /vntifkey="47"
                     /label=putative\-10
     primer          2609..2628
                     /vntifkey="27"
                     /label=primer\1550
BASE COUNT     1415 a      2196 c      2121 g      1600 t 
ORIGIN
        1 atcgatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca 
       61 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca 
      121 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc 
      181 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca 
      241 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc 
      301 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg 
      361 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg 
      421 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag 
      481 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt 
      541 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc 
      601 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc 
      661 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg 
      721 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc 
      781 agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc gtaattctga 
      841 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc 
      901 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt 
      961 atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc 
     1021 ggcggccaat cttgctcgtc tcgctggccg gcgccactgt cgactacgcc atcatggcga 
     1081 cagcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga 
     1141 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc 
     1201 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg 
     1261 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc 
     1321 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt 
     1381 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg 
     1441 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc 
     1501 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc 
     1561 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg 
     1621 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca 
     1681 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt 
     1741 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac 
     1801 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac 
     1861 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga 
     1921 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg 
     1981 gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggaaacgtgg ccaatatgga 
     2041 caacttcttc gcatcgatct gcacttgaac gtgtggccta agcgatatct taggatctcc 
     2101 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 
     2161 ccacgatgcg tccggcgtag aggatctgct catgtttgac agcttactcg agtatgctag 
     2221 cgatgagctc gcacgaaccc agttgacata agcctgttcg gttcgtaaac tgtaatgcaa 
     2281 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg 
     2341 cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc 
     2401 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga 
     2461 tgttacgcag cagggcagtc gccctaaaac aaagttaggc agccgttgtg ctggtgcttt 
     2521 ctgatagttg ttgtggggta ggcagtcaga gctcgatttg cttgtcgcca taatagattc 
     2581 acaagaagga ttcggaattc tacatatgtc atcgacacca tggtacccgg ggatcctcta 
     2641 gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc 
     2701 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca 
     2761 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg 
     2821 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga 
     2881 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 
     2941 ctgagtagga caaatccgcc gggagcggat ttgaacgctg cgaagcaacg gcccggaggg 
     3001 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg 
     3061 acggatggcc tttttgcgtt tctacaaact cttccactca ctacagcaga gccatttaaa 
     3121 caacatcccc tccccctttc caccgcgtca gacgcccgta gcagcccgct acgggctttt 
     3181 tcatgccctg ccctagcgtc caagcctcac ggccgcgctc ggcctctctg gcggccttct 
     3241 ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc 
     3301 catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc 
     3361 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg 
     3421 tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca 
     3481 agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc 
     3541 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg 
     3601 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag 
     3661 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc 
     3721 ctcgtcaaaa ataaggttat caagcgagaa atcaccatga gtgacgactg aatccggtga 
     3781 gaatggcaaa agctaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt 
     3841 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg 
     3901 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata 
     3961 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta 
     4021 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag 
     4081 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcgaaga 
     4141 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 
     4201 tagacgtcag gtggcacttt tcggggaaat gtgcgcgccc gcgttcctgc tggcgctggg 
     4261 cctgtttctg gcgctggact tcccgctgtt ccgtcagcag cttttcgccc acggccttga 
     4321 tgatcgcggc ggccttggcc tgcatatccc gattcaacgg ccccagggcg tccagaacgg 
     4381 gcttcaggcg ctcccgaagg tctcgggccg tctcttgggc ttgatcggcc ttcttgcgca 
     4441 tctcacgcgc tcctgcggcg gcctgtaggg caggctcata cccctgccga accgcttttg 
     4501 tcagccggtc ggccacggct tccggcgtct caacgcgctt tgagattccc agcttttcgg 
     4561 ccaatccctg cggtgcatag gcgcgtggct cgaccgcttg cgggctgatg gtgacgtggc 
     4621 ccactggtgg ccgctccagg gcctcgtaga acgcctgaat gcgcgtgtga cgtgccttgc 
     4681 tgccctcgat gccccgttgc agccctagat cggccacagc ggccgcaaac gtggtctggt 
     4741 cgcgggtcat ctgcgctttg ttgccgatga actccttggc cgacagcctg ccgtcctgcg 
     4801 tcagcggcac cacgaacgcg gtcatgtgcg ggctggtttc gtcacggtgg atgctggccg 
     4861 tcacgatgcg atccgccccg tacttgtccg ccagccactt gtgcgccttc tcgaagaacg 
     4921 ccgcctgctg ttcttggctg gccgacttcc accattccgg gctggccgtc atgacgtact 
     4981 cgaccgccaa cacagcgtcc ttgcgccgct tctctggcag caactcgcgc agtcggccca 
     5041 tcgcttcatc ggtgctgctg gccgcccagt gctcgttctc tggcgtcctg ctggcgtcag 
     5101 cgttgggcgt ctcgcgctcg cggtaggcgt gcttgagact ggccgccacg ttgcccattt 
     5161 tcgccagctt cttgcatcgc atgatcgcgt atgccgccat gcctgcccct cccttttggt 
     5221 gtccaaccgg ctcgacgggg gcagcgcaag gcggtgcctc cggcgggcca ctcaatgctt 
     5281 gagtatactc actagacttt gcttcgcaaa gtcgtgaccg cctacggcgg ctgcggcgcc 
     5341 ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt gccagcccgt 
     5401 ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc ggcccgtgga 
     5461 caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg accacaggga 
     5521 ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg cgcggcctgc 
     5581 ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc ctgcggcctg 
     5641 cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg cgcagcgacc 
     5701 gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag tgggggcagt 
     5761 cgaaggcgaa gcccgcccgc ctgccccccg agcctcacgg cggcgagtgc gggggttcca 
     5821 agggggcagc gccaccttgg gcaaggccga aggccgcgca gtcgatcaac aagccccgga 
     5881 ggggccactt tttgccggag ggggagccgc gccgaaggcg tgggggaacc ccgcaggggt 
     5941 gcccttcttt gggcaccaaa gaactagata tagggcgaaa tgcgaaagac ttaaaaatca 
     6001 acaacttaaa aaaggggggt acgcaacagc tcattgcggc accccccgca atagctcatt 
     6061 gcgtaggtta aagaaaatct gtaattgact gccactttta cgcaacgcat aattgttgtc 
     6121 gcgctgccga aaagttgcag ctgattgcgc atggtgccgc aaccgtgcgg caccctaccg 
     6181 catggagata agcatggcca cgcagtccag agaaatcggc attcaagcca agaacaagcc 
     6241 cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg tgggccgggc ttattgcgag 
     6301 gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg cagatgggcc accagaacgc 
     6361 cgtggtggtc agccagaaga cactttccaa gctcatcgga cgttctttgc ggacggtcca 
     6421 atacgcagtc aaggacttgg tggccgagcg ctggatctcc gtcgtgaagc tcaacggccc 
     6481 cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg tggggccagc cccgcgacca 
     6541 gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat cacgacgacc aggacgaatc 
     6601 gctgttgggg catggcgacc tgcgccgcat cccgaccctg tatccgggcg agcagcaact 
     6661 accgaccggc cccggcgagg agccgcccag ccagcccggc attccgggca tggaaccaga 
     6721 cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc gggcagcagc gcctgccgat 
     6781 gcccgatgag ccgtgttttc tggacgatgg cgagccgttg gagccgccga cacgggtcac 
     6841 gctgccgcgc cggtagcact tgggttgcgc agcaacccgt aagtgcgctg ttccagacta 
     6901 tcggctgtag ccgcctcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg 
     6961 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac 
     7021 cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta ttacctccac 
     7081 ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca cactgcttcc 
     7141 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc 
     7201 tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca 
     7261 cttattcagg cgtagcacca ggcgtttaag tcgaccaata actctacggg gtctgacgct 
     7321 cagtggaacg aa 
//
