LOCUS Exported 6111 bp ds-DNA circular SYN 14-JUN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6111) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 3, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6111 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 229..250 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 265..295 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 303..319 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 327..343 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 592..1038 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" CDS 1055..1108 /codon_start=1 /label=NES /translation="MLQNELALKLAGLDINKT" CDS 1121..2608 /codon_start=1 /label=NIR-GECO1 /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV IQASINAAEFLNTNFVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD CTIHRPSNGGLIVELEPATKATNIAPALVGALHRITSSSSLMGLCDETATIFREITGFD RVMVMRLGALDDLTEEQIAEIKEAFSLFDKDGDGTITTKELGTVFRSLGQNPTEAELQD MINEVDADGDGTFDFPEFLTMMARKMNDSDSEEEIREAFRVFDKDGNGYIGAAELRHVM TDLGEKLTDEEVDEMIRVADNDGDGQVNYEEFVQMMTAKGGGGSVDSSRRKWNKAGHAV RAIGRLSSRRHDLLSECRRADLEAFLGNRYPASTIPQIARRLYELNRVRLLVDVNYTPV PLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIVCHH YEPRFVPSHIRAAGEALAETCANRIATLESFAQSQSK" misc_feature 2621..3209 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(3092..3103) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(3212..3228) /label=KS primer /note="common sequencing primer, one of multiple similar variants" polyA_signal 3258..3379 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3689..3705) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 3847..4302 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4328..4432 /gene="bla" /label=AmpR promoter CDS 4433..5293 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5464..6052 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt 361 taattaaggc cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc 421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg 481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact 541 tatctacgta gccatgctct aggaagatct ctgcagaggg ccctgcgtat gagtgcaagt 601 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccaccca 661 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg 721 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct 781 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca 841 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc 901 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg 961 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg 1021 tcgtgcctga gagcgcagtc gaattcgggg atccatgctg cagaacgagc ttgctcttaa 1081 gttggctgga cttgatatta acaagactgg aggaggttct atgtcggtac cgctgactac 1141 ctcagcattc ggccacgcgt ttctggctaa ctgtgaacgt gagcagatcc acctggcggg 1201 ctccattcag ccgcacggta tcctgctggc tgttaaagag cctgacaacg tggtgatcca 1261 ggcttctatt aacgctgcgg agttcctgaa caccaacttt gttgttggcc gtccgctgcg 1321 tgacctgggc ggcgatctgc ctttgcagat cctgccgcac ctgaacggcc cgctgcacct 1381 ggctccgatg accctgcgtt gtactgtggg ttctccgccg cgtcgtgtgg actgtaccat 1441 tcatcgaccg tctaacggcg gcctgatcgt agaactggaa ccagcaacca aggccactaa 1501 cattgcgccg gctctggtcg gtgcgcttca tcgtatcact tcttcatcct ccctgatggg 1561 cctgtgtgac gaaaccgcga ctattttccg tgagattact ggcttcgacc gtgtgatggt 1621 aatgcgtctc ggcgcgcttg acgatctgac tgaagagcag atcgcagaga ttaaagaggc 1681 tttctcccta tttgacaagg acggggacgg gacgataaca accaaggagc tggggacggt 1741 gttccggtct ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt 1801 agatgccgat ggcgacggca cattcgactt ccctgagttc ctgacgatga tggcaaggaa 1861 aatgaatgac tcagacagtg aagaggaaat tagagaagcg ttccgcgtgt ttgataagga 1921 cggcaatggc tacatcggcg cagcagagct tcgccacgtg atgacagacc ttggtgagaa 1981 gttaactgat gaggaggttg atgaaatgat cagggtagca gacaacgatg gggatggtca 2041 ggtaaactac gaagagtttg tacaaatgat gacagcgaag ggtggcggag gttctgtaga 2101 ttcatcacgt cgtaagtgga ataaggcagg tcacgcagtc agagctatag gtcggctgag 2161 ctcgcgtagg catgatttgc tgtccgaatg tcgtcgtgcg gacctggaag cgttcctggg 2221 taaccgctac ccggcgtcta ctattccgca gatcgctcgt cgcctgtacg aacttaaccg 2281 tgttcgcctg ctggtagatg tgaactatac tccggttccg ctacagccgc gcatcagccc 2341 gctgaacggt cgtgatctgg atatgtccct gtcttgcctg cgctctatgt ccccgatcca 2401 ccagaaatac atgcaggaca tgggcgttgg cgcaaccctg gtttgctctc tgatggtgtc 2461 tggtcgtctg tggggtctga tcgtttgcca ccactacgaa ccgcgcttcg ttccgtccca 2521 cattcgcgct gctggcgaag cgctggcgga aacttgtgcg aaccgcatcg cgacgctgga 2581 gagctttgca cagtctcagt ccaaatgaaa gcttatcgat aatcaacctc tggattacaa 2641 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 2701 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 2761 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 2821 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 2881 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 2941 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 3001 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 3061 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 3121 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 3181 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac ccgggcggcc 3241 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 3301 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 3361 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 3421 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa 3481 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca 3541 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 3601 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 3661 gagcgcgcag ccttaattaa cctaattcac tggccgtcgt tttacaacgt cgtgactggg 3721 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 3781 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 3841 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 3901 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 3961 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 4021 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 4081 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 4141 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 4201 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 4261 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 4321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 4381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 4441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 4501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 4561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 4621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 4681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 4741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 4801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 4861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 4921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 4981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 5041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 5101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 5161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 5221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 5281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 5341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 5401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 5461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 5521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 5581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 5641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 5701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 5761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 5821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 5881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 5941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 6001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 6061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca t //