LOCUS       pNS38-SadCas9-SNAP      9758 bp    DNA     circular UNA 29-Jun-2015
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             5323..8481
                     /cds_type=ORF
                     /label=SaCas9
     CDS             5350..5352
                     /cds_type=ORF
                     /label="Mutation Y10A"
     CDS             7060..7062
                     /cds_type=ORF
                     /label="Mutation Y580A"
     NLS             8512..8640
     CDS             8641..9192
                     /cds_type=ORF
                     /label=SNAP26
     CDS             9193..9225
                     /cds_type=ORF
                     /label=nls
ORIGIN
        1 TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT
       61 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
      121 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
      181 GCTTCAATAA CATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
      241 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
      301 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
      361 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
      421 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG
      481 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
      541 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
      601 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
      661 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
      721 ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT
      781 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
      841 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
      901 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG
      961 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
     1021 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
     1081 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
     1141 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
     1201 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
     1261 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
     1321 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
     1381 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
     1441 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
     1501 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
     1561 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
     1621 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
     1681 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
     1741 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
     1801 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
     1861 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
     1921 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
     1981 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA
     2041 TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC
     2101 GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC
     2161 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT
     2221 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC
     2281 CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA
     2341 TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC
     2401 TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG
     2461 TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA
     2521 CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC
     2581 TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG
     2641 TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA
     2701 ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA
     2761 AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC
     2821 GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG
     2881 TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA
     2941 TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG
     3001 TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC
     3061 CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG
     3121 ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT
     3181 CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT
     3241 TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG
     3301 CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT
     3361 AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC
     3421 GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT
     3481 GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT
     3541 CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG
     3601 TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG
     3661 CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC
     3721 CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC
     3781 CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC
     3841 TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC
     3901 CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC
     3961 GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAATT AATACGACTC
     4021 ACTATAGGGA GACCACAACG GTTTCCCTCT AGTGCCGGCT CCGGAGAGCT CTTTAATTAA
     4081 GCGGCCGCCC TGCAGGACTC GAGTTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT
     4141 ATACATATGA AATCTTCTCA CCATCACCAT CACCATGGTT CTTCTATGAA AATCGAAGAA
     4201 GGTAAACTGG TAATCTGGAT TAACGGCGAT AAAGGCTATA ACGGTCTCGC TGAAGTCGGT
     4261 AAGAAATTCG AGAAAGATAC CGGAATTAAA GTCACCGTTG AGCATCCGGA TAAACTGGAA
     4321 GAGAAATTCC CACAGGTTGC GGCAACTGGC GATGGCCCTG ACATTATCTT CTGGGCACAC
     4381 GACCGCTTTG GTGGCTACGC TCAATCTGGC CTGTTGGCTG AAATCACCCC GGACAAAGCG
     4441 TTCCAGGACA AGCTGTATCC GTTTACCTGG GATGCCGTAC GTTACAACGG CAAGCTGATT
     4501 GCTTACCCGA TCGCTGTTGA AGCGTTATCG CTGATTTATA ACAAAGATCT GCTGCCGAAC
     4561 CCGCCAAAAA CCTGGGAAGA GATCCCGGCG CTGGATAAAG AACTGAAAGC GAAAGGTAAG
     4621 AGCGCGCTGA TGTTCAACCT GCAAGAACCG TACTTCACCT GGCCGCTGAT TGCTGCTGAC
     4681 GGGGGTTATG CGTTCAAGTA TGAAAACGGC AAGTACGACA TTAAAGACGT GGGCGTGGAT
     4741 AACGCTGGCG CGAAAGCGGG TCTGACCTTC CTGGTTGACC TGATTAAAAA CAAACACATG
     4801 AATGCAGACA CCGATTACTC CATCGCAGAA GCTGCCTTTA ATAAAGGCGA AACAGCGATG
     4861 ACCATCAACG GCCCGTGGGC ATGGTCCAAC ATCGACACCA GCAAAGTGAA TTATGGTGTA
     4921 ACGGTACTGC CGACCTTCAA GGGTCAACCA TCCAAACCGT TCGTTGGCGT GCTGAGCGCA
     4981 GGTATTAACG CCGCCAGTCC GAACAAAGAG CTGGCAAAAG AGTTCCTCGA AAACTATCTG
     5041 CTGACTGATG AAGGTCTGGA AGCGGTTAAT AAAGACAAAC CGCTGGGTGC CGTAGCGCTG
     5101 AAGTCTTACG AGGAAGAGTT GGCGAAAGAT CCACGTATTG CCGCCACTAT GGAAAACGCC
     5161 CAGAAAGGTG AAATCATGCC GAACATCCCG CAGATGTCCG CTTTCTGGTA TGCCGTGCGT
     5221 ACTGCGGTGA TCAACGCCGC CAGCGGTCGT CAGACTGTCG ATGAAGCCCT GAAAGACGCG
     5281 CAGACTAATG GGATCGAGGA AAACCTGTAC TTCCAATCCA ATATGAAAAG GAATTATATC
     5341 TTAGGATTAg cTATCGGAAT TACATCAGTG GGTTATGGAA TTATTGATTA TGAAACTAGA
     5401 GATGTCATAG ATGCGGGCGT ACGTTTATTT AAAGAGGCTA ATGTTGAAAA TAATGAAGGA
     5461 CGACGATCAA AAAGAGGTGC CAGAAGGCTT AAGAGGCGTC GTAGACATAG AATACAAAGA
     5521 GTAAAGAAAC TTTTATTTGA TTACAATTTG TTGACAGATC ATAGTGAGCT AAGTGGAATC
     5581 AATCCTTACG AGGCGCGCGT AAAGGGATTA AGTCAAAAAT TAAGTGAAGA GGAATTTTCT
     5641 GCGGCATTGC TACATTTAGC AAAGCGTAGA GGTGTACATA ATGTTAATGA AGTGGAAGAA
     5701 GATACAGGTA ATGAATTATC CACTAAAGAA CAAATTTCAA GAAATAGTAA AGCGTTAGAA
     5761 GAGAAGTATG TTGCAGAATT ACAGTTGGAA CGTTTGAAAA AAGACGGTGA AGTGAGAGGT
     5821 TCGATTAACC GTTTCAAAAC ATCTGACTAT GTAAAAGAAG CAAAGCAGTT ATTAAAAGTA
     5881 CAAAAAGCAT ATCATCAACT TGATCAATCA TTTATAGACA CTTATATTGA TTTATTGGAA
     5941 ACAAGAAGAA CATATTATGA GGGACCAGGT GAAGGTAGCC CATTTGGATG GAAAGATATT
     6001 AAAGAATGGT ATGAAATGTT AATGGGACAT TGTACGTATT TCCCAGAAGA ATTACGTAGT
     6061 GTGAAATATG CCTATAATGC TGATTTATAT AATGCGCTGA ATGATTTGAA CAACTTGGTT
     6121 ATTACACGAG ATGAGAATGA GAAGCTAGAG TATTATGAAA AATTCCAAAT TATCGAGAAT
     6181 GTCTTTAAAC AAAAGAAAAA GCCGACGCTT AAACAAATTG CGAAGGAAAT CTTGGTGAAT
     6241 GAAGAAGACA TCAAAGGCTA TCGTGTCACA AGTACAGGTA AACCAGAATT TACAAACTTG
     6301 AAAGTTTATC ACGATATCAA AGATATTACA GCAAGAAAAG AAATTATCGA GAATGCAGAG
     6361 CTACTCGATC AAATAGCTAA AATATTAACT ATTTACCAGT CATCAGAAGA TATACAAGAA
     6421 GAATTAACAA ACCTAAATTC AGAATTGACA CAAGAAGAGA TTGAACAAAT TTCAAACTTG
     6481 AAAGGTTATA CAGGAACTCA TAACCTTTCA CTAAAGGCAA TAAATTTAAT ATTAGACGAA
     6541 TTGTGGCATA CGAACGATAA TCAAATAGCT ATTTTCAATC GTTTGAAACT TGTACCTAAA
     6601 AAGGTAGATT TAAGCCAACA AAAAGAAATT CCTACTACTT TAGTTGATGA TTTTATACTG
     6661 TCTCCAGTAG TGAAACGTTC ATTTATACAA TCTATTAAAG TTATTAACGC TATTATTAAA
     6721 AAATACGGTT TGCCAAATGA TATTATTATT GAACTTGCGA GAGAAAAGAA TTCTAAAGAT
     6781 GCACAAAAAA TGATTAATGA AATGCAGAAG AGAAATCGTC AAACGAATGA ACGTATTGAG
     6841 GAAATTATAA GAACGACAGG TAAAGAAAAT GCTAAATATT TAATTGAAAA AATTAAGCTG
     6901 CACGATATGC AAGAAGGGAA ATGTTTATAC TCGTTAGAAG CAATCCCTCT AGAAGATTTA
     6961 CTTAATAATC CATTCAATTA CGAAGTAGAC CATATCATTC CACGTTCTGT TTCTTTCGAT
     7021 AACTCTTTCA ATAATAAAGT GTTGGTGAAA CAAGAAGAAg cTAGTAAAAA AGGTAATAGA
     7081 ACGCCATTTC AATATTTAAG TTCTTCAGAT TCTAAAATAA GTTATGAGAC ATTCAAAAAG
     7141 CATATTTTAA ATCTTGCTAA AGGCAAAGGT AGAATCTCTA AGACGAAAAA AGAATATTTG
     7201 TTAGAAGAAC GAGATATCAA TCGCTTCAGT GTCCAAAAAG ATTTTATTAA CCGTAACTTA
     7261 GTAGATACAC GCTATGCGAC AAGAGGGTTA ATGAACTTAT TAAGATCTTA TTTTAGAGTG
     7321 AATAACTTAG ATGTCAAAGT GAAATCGATT AATGGCGGAT TCACAAGTTT CTTAAGAAGG
     7381 AAATGGAAGT TCAAAAAAGA AAGAAATAAG GGCTACAAAC ACCATGCTGA AGATGCACTG
     7441 ATTATTGCGA ACGCTGATTT TATTTTCAAA GAATGGAAAA AACTAGATAA AGCTAAAAAA
     7501 GTGATGGAAA ATCAAATGTT TGAAGAAAAG CAAGCTGAAA GTATGCCTGA AATTGAGACT
     7561 GAGCAAGAGT ATAAAGAAAT TTTTATAACG CCTCATCAAA TTAAACATAT TAAGGATTTT
     7621 AAAGATTATA AATATTCACA TAGAGTTGAT AAAAAGCCGA ATAGAGAGTT AATAAATGAT
     7681 ACATTATATT CTACGAGAAA AGATGACAAG GGTAATACAT TAATCGTTAA TAACTTAAAT
     7741 GGTTTATACG ATAAAGATAA TGATAAATTG AAAAAATTAA TTAATAAATC ACCTGAAAAA
     7801 TTATTGATGT ATCATCATGA TCCACAAACA TATCAAAAAT TAAAATTGAT CATGGAACAA
     7861 TATGGCGATG AGAAAAATCC GCTTTATAAA TATTATGAAG AAACAGGCAA TTACTTAACA
     7921 AAATATAGTA AAAAAGATAA CGGACCAGTC ATCAAAAAAA TTAAATATTA TGGTAACAAG
     7981 CTAAATGCGC ATTTAGATAT TACGGATGAT TATCCAAATA GCAGAAATAA AGTAGTAAAA
     8041 CTTTCATTAA AACCATATCG CTTTGATGTT TATTTAGATA ATGGGGTATA TAAATTTGTG
     8101 ACAGTTAAAA ATTTAGATGT TATCAAAAAA GAAAACTACT ATGAAGTTAA TTCAAAGTGT
     8161 TATGAAGAAG CAAAAAAACT GAAGAAAATT AGTAATCAAG CAGAATTTAT CGCAAGTTTT
     8221 TACAATAATG ACTTGATTAA GATTAACGGA GAATTATATA GAGTCATAGG TGTAAATAAT
     8281 GATCTACTTA ACAGAATTGA AGTAAATATG ATAGACATCA CATATAGAGA ATATTTAGAG
     8341 AACATGAATG ATAAAAGACC ACCTAGAATA ATTAAAACAA TAGCAAGCAA AACACAATCT
     8401 ATTAAAAAGT ATTCTACAGA TATTCTAGGC AATCTTTATG AAGTGAAGAG TAAAAAGCAT
     8461 CCTCAAATCA TAAAGAAAGG ATACGAACAT TGGAAGTGGA TAACGGATCC AGCCTATCCC
     8521 TATGACGTGC CCGATTATGC CAGCCTGGGC AGCGGCTCCC CCAAGAAAAA ACGCAAGGTG
     8581 GAAGATCCTA AGAAAAAGCG GAAAGTGGAC GGCATTGGTA GTGGGAGCAA CGGCAGCAGC
     8641 GACAAAGATT GCGAAATGAA ACGTACCACC CTGGATAGCC CGCTGGGCAA ACTGGAACTG
     8701 AGCGGCTGCG AACAGGGCCT GCATGAAATT AAACTGCTGG GTAAAGGCAC CAGCGCGGCC
     8761 GATGCGGTTG AAGTTCCGGC CCCGGCCGCC GTGCTGGGTG GTCCGGAACC GCTGATGCAG
     8821 GCGACCGCGT GGCTGAACGC GTATTTTCAT CAGCCGGAAG CGATTGAAGA ATTTCCGGTT
     8881 CCGGCGCTGC ATCATCCGGT GTTTCAGCAG GAGAGCTTTA CCCGTCAGGT GCTGTGGAAA
     8941 CTGCTGAAAG TGGTTAAATT TGGCGAAGTG ATTAGCTATC AGCAGCTGGC GGCCCTGGCG
     9001 GGTAATCCGG CGGCCACCGC CGCCGTTAAA ACCGCGCTGA GCGGTAACCC GGTGCCGATT
     9061 CTGATTCCGT GCCATCGTGT GGTTAGCTCT AGCGGTGCGG TTGGCGGTTA TGAAGGTGGT
     9121 CTGGCGGTGA AAGAGTGGCT GCTGGCCCAT GAAGGTCATC GTCTGGGTAA ACCGGGTCTG
     9181 GGACCTGCAG GCGGGTCACC TAAGAAAAAA CGAAAAGTTG AGTAAGGTAC CACGCGTGCG
     9241 CGCTGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA
     9301 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA
     9361 AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA
     9421 TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG
     9481 CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT
     9541 AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA
     9601 TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT
     9661 TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC
     9721 ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
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