LOCUS 3942_aav-CAG-Fle 7172 bp DNA linear 05-JUL-2018 FEATURES Location/Qualifiers misc_feature 5372..6229 /note="Amp Resistance" misc_feature complement(4547..4714) /note="ITR" promoter complement(3585..4520) /note="CBA promoter" misc_feature complement(3445..3478) /note="loxP" misc_feature 3541..3574 /note="loxP2272" insert complement(3440..3443) /dnas_title="AAV21_Syn_FLEX_WPRE-MCS-iGluSnFr(reverse) %1E%1F" /source="AAV21_Syn_FLEX_WPRE-MCS-iGluSnFr(reverse)" misc_feature complement(511..1099) /note="WPRE" misc_feature complement(1133..1166) /note="loxP" misc_feature complement(251..483) /note="SV40" misc_feature complement(57..223) /note="ITR" insert complement(1271..1277) /dnas_title="AAV21_Syn_FLEX_WPRE-MCS-iGluSnFr(reverse)" /source="AAV21_Syn_FLEX_WPRE-MCS-iGluSnFr(reverse)" misc_feature complement(1237..1270) /note="loxP2272" misc_feature 1298..1948 /label=NfsB misc_feature 1667..1669 /label=F124W misc_feature 2015..2665 /label=NfsB misc_feature 2384..2386 /label=F124W misc_feature 2684..3391 /label=mCherry source 1..7172 /dnas_title="3942_aav-CAG-Flex-tdNfsB(F124W)-mCherry (eNTR)" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg 61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg 121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg 181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa 241 gatccttatc gattttacca catttgtaga ggttttactt gctttaaaaa acctcccaca 301 tctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc 481 gaagcggccg cccgggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac 541 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg 601 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa 661 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca 721 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga 781 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg 841 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac 901 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg 961 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt 1021 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt 1081 tgtaatccag aggttgatta tcgataAGCT aaTTCGAACC TCTTCGAGGG ACATAACTTC 1141 GTATAATGTA TGCTATACGA AGTTATGGGT CGATGGTGAG ATCTGGACTA GAGGGTCGAT 1201 GGTGATGCTT GGATCAAAAT AGGAAGAATC CCAATGATAA CTTCGTATAA AGTATCCTAT 1261 ACGAAGTTAT ggatccGaat tctgaactag tgcaggtatg gatatcatct cggtggcatt 1321 gaaacgtcat tctaccaaag ccttcgatgc gtcgaaaaag cttacgccag agcaagctga 1381 acagatcaag acgttgcttc agtattcacc cagttcaact aattcccaac cctggcactt 1441 catcgtggcg tccacggagg aagggaaagc gcgagtagcg aaatcagcag ccggcaacta 1501 tgtctttaac gaacgcaaaa tgctcgatgc aagccacgtg gtggtctttt gtgccaaaac 1561 cgcaatggac gatgtatggc ttaagctggt agtggaccaa gaggatgcgg atggaaggtt 1621 cgctacaccg gaggcgaaag ccgcgaatga taagggaaga aagttctGGg cagacatgca 1681 tcgaaaagac ctccatgatg acgcggagtg gatggccaag caggtttacc ttaatgtagg 1741 taacttcctg ttgggggtcg ccgcgttggg gctggacgct gtgcctattg aaggttttga 1801 cgccgccatt ttggatgcag agtttggact caaagaaaaa ggctacacta gcctcgtggt 1861 ggttccggtc ggtcaccact cggtggagga ctttaacgcg accctcccca agtcgcggct 1921 gccgcagaat atcacactca cggaagtggc tggttctagt gcaggtcaag gtgcacaggc 1981 tggtcaagga gcacaggctg gttctagtgc aggtatggat atcatctcgg tggcattgaa 2041 acgtcattct accaaagcct tcgatgcgtc gaaaaagctt acgccagagc aagctgaaca 2101 gatcaagacg ttgcttcagt attcacccag ttcaactaat tcccaaccct ggcacttcat 2161 cgtggcgtcc acggaggaag ggaaagcgcg agtagcgaaa tcagcagccg gcaactatgt 2221 ctttaacgaa cgcaaaatgc tcgatgcaag ccacgtggtg gtcttttgtg ccaaaaccgc 2281 aatggacgat gtatggctta agctggtagt ggaccaagag gatgcggatg gaaggttcgc 2341 tacaccggag gcgaaagccg cgaatgataa gggaagaaag ttctGGgcag acatgcatcg 2401 aaaagacctc catgatgacg cggagtggat ggccaagcag gtttacctta atgtaggtaa 2461 cttcctgttg ggggtcgccg cgttggggct ggacgctgtg cctattgaag gttttgacgc 2521 cgccattttg gatgcagagt ttggactcaa agaaaaaggc tacactagcc tcgtggtggt 2581 tccggtcggt caccactcgg tggaggactt taacgcgacc ctccccaagt cgcggctgcc 2641 gcagaatatc acactcacgg aagtggctgg ttctagtgca ggtatggtga gcaagggcga 2701 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 2761 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2821 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2881 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2941 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 3001 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 3061 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 3121 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 3181 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 3241 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 3301 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 3361 ctccaccggc ggcatggacg agctgtacaa ggctggttct agtCTCGAGt aaGATatcac 3421 cggtggcgcg ccAAGCTTgC TAGTATAACT TCGTATAGCA TACATTATAC GAAGTTATGG 3481 GTCGATGGTG ATGCTTGGCA ATTCGGGTCG ATGGTGAAGC ATTGGTCTTC CTATTTTGAT 3541 ATAACTTCGT ATAGGATACT TTATACGAAG TTATGGGgat GATCcaattc tttgccaaaa 3601 tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca 3661 tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc 3721 ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagc cgctcccccc 3781 gcacaaaggg ccctcccgga gcccctcaag gctttcacgc agccacagaa aagaaacaag 3841 ccgtcattaa accaagcgct aattacagcc cggaggagaa gggccgtccc gcccgctcac 3901 ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg 3961 gggcacgggg cgaaggcagc gtcgcagcga ctcccgcccg ccgcgcgctt cgctttttat 4021 agggccgccg ccgccgccgc ctcgccataa aaggaaactt tcggagcgcg ccgctctgat 4081 tggctgccgc cgcacctctc cgcctcgccc cgccccgccc ctcgccccgc cccgccccgc 4141 ctggcgcgcg cccccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa 4201 ataaatacaa aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg 4261 ggctcacctc gaccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa 4321 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt 4381 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc 4441 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat 4501 acgtcattat tgacgtcaat ggtacgatct tcctagagca tggctacgta gataagtagc 4561 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc 4621 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg 4681 cccgggcggc ctcagtgagc gagcgagcgc gcagctggcg taatagcgaa gaggcccgca 4741 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 4801 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 4861 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 4921 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 4981 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 5041 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 5101 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 5161 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 5221 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 5281 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 5341 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 5401 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 5461 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 5521 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 5581 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 5641 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 5701 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 5761 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 5821 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 5881 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 5941 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 6001 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 6061 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 6121 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 6181 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 6241 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 6301 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 6361 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 6421 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 6481 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 6541 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 6601 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 6661 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 6721 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 6781 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 6841 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 6901 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 6961 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 7021 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 7081 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 7141 gcgcagcgag tcagtgagcg aggaagcgga ag //