LOCUS       ALT3943_aavSYN-F        6988 bp    DNA     linear       27-AUG-2018
FEATURES             Location/Qualifiers
     PCR_primer      6860..6883
                     /pair="(f.ITR) 1.14"
                     /primer="CTCACATGTTCTTTCCTGCGTTAT"
                     /current=0
     misc_feature    4363..4530
                     /note="ITR"
     PCR_primer      complement(4698..4721)
                     /pair="(r.ITR) 1.20"
                     /primer="TGGCGAGAAAGGAAGGGAAGAAAG"
                     /current=0
     misc_feature    complement(3811..4298)
                     /note="synapsin promoter"
     PCR_primer      4254..4276
                     /pair="(r.ITR) 1.17"
                     /primer="GAATGGGGGTTGGGTGCTTGTCC"
                     /current=0
     PCR_primer      complement(3921..3941)
                     /pair="f.hsyn.seqforGECI.01.7"
                     /primer="CGGTGGGCAGCGGAGGAGTCG"
                     /current=0
     misc_feature    complement(join(1187..1348,3533..3670))
                     /dnas_title="Flex%1E%1F%1E"
                     /note="Flex"
     insert          complement(join(1349..1351,3511..3532))
                     /dnas_title="MCS_2%1E%1F%1E"
                     /source="MCS_2"
     insert          join(1352..1356,3505..3510)
                     /source="GCaMP6f primer design"
                     /type="Custom cloned insert"
                     /dnas_title="GCaMP6f primer design%1E%1F%1E"
     misc_feature    complement(3534..3567)
                     /note="loxp"
     insert          3490..3504
                     /source="oligo STOP"
                     /type="Custom cloned insert"
     misc_feature    complement(3671..3810)
                     /note="Intron"
     misc_feature    complement(3630..3663)
                     /note="loxp2272"
     misc_feature    2769..3476
                     /label=mCherry
     misc_feature    2100..2750
                     /label=NfsB
     misc_feature    2469..2471
                     /label=F124W
     misc_feature    1383..2033
                     /label=NfsB
     misc_feature    1752..1754
                     /label=F124W
     misc_feature    complement(519..1134)
                     /note="wpre"
     PCR_primer      1044..1063
                     /pair="r.wpre.rev.01.3"
                     /primer="TTAAAGCAGCGTATCCACAT"
                     /current=0
     misc_feature    1310..1343
                     /note="loxp2272"
     misc_feature    1206..1239
                     /note="loxp"
     insert          complement(1357..1362)
                     /dnas_title="AAV21_Syn_FLEX_WPRE-MCS-iGluSnFr(reverse)"
                     /source="AAV21_Syn_FLEX_WPRE-MCS-iGluSnFr(reverse)"
     misc_feature    56..223
                     /note="ITR"
     misc_feature    251..483
                     /note="SV40"
     PCR_primer      complement(307..327)
                     /pair="(f.ITR) 1.16"
                     /primer="ATTTTATGTTTCAGGTTCAGG"
                     /current=0
     PCR_primer      complement(869..887)
                     /pair="f.wpre.seq.01.50"
                     /primer="GCATTGCCACCACCTGTCA"
                     /current=0
     PCR_primer      3921..3941
                     /pair="r.syn.seq.01.52"
                     /primer="CGACTCCTCCGCTGCCCACCG"
                     /current=0
     PCR_primer      4049..4065
                     /pair="r.synseq.01.53"
                     /primer="CGGACGCGACCAAGGTG"
                     /current=0
     PCR_primer      complement(4745..4763)
                     /pair="r.ITRrev3.01.19"
                     /primer="GGAGCCCCCGATTTAGAGC"
                     /current=1
     source          1..6988
                     /dnas_title="ALT3943_aavSYN-Flex"
ORIGIN      
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGCG
       61 CGCTCGCTCG CTCACTGAGG CCGCCCGGGC AAAGCCCGGG CGTCGGGCGA CCTTTGGTCG
      121 CCCGGCCTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA TCACTAGGGG
      181 TTCCTTGTAG TTAATGATTA ACCCGCCATG CTACTTATCt acgtagccat gctctaggaa
      241 gatccttatc gattttacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      301 tctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc
      481 gaagcggccg cccgggtcga ctctagaTGC ATGCTCGAgc ggccgcccgg gtcgacggta
      541 tcgatgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg
      601 cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc
      661 cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag
      721 ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg
      781 agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag
      841 ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg
      901 ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc
      961 ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga
     1021 agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac
     1081 atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt gattatcgat
     1141 aagcttctcg agtggatcaT CTGCAGAATT CGCCCTTttc agtgtttCGA ACCTCTTCGA
     1201 GGGACATAAC TTCGTATAAT GTATGCTATA CGAAGTTATG GGTCGATGGT GAGATCTGGA
     1261 CTAGAGGGTC GATGGTGATG CTTGGATCAA AATAGGAAGA ATCCCAATGA TAACTTCGTA
     1321 TAAAGTATCC TATACGAAGT TATACTAGTc atatgggatc cGaattctga actagtgcag
     1381 gtatggatat catctcggtg gcattgaaac gtcattctac caaagccttc gatgcgtcga
     1441 aaaagcttac gccagagcaa gctgaacaga tcaagacgtt gcttcagtat tcacccagtt
     1501 caactaattc ccaaccctgg cacttcatcg tggcgtccac ggaggaaggg aaagcgcgag
     1561 tagcgaaatc agcagccggc aactatgtct ttaacgaacg caaaatgctc gatgcaagcc
     1621 acgtggtggt cttttgtgcc aaaaccgcaa tggacgatgt atggcttaag ctggtagtgg
     1681 accaagagga tgcggatgga aggttcgcta caccggaggc gaaagccgcg aatgataagg
     1741 gaagaaagtt ctGGgcagac atgcatcgaa aagacctcca tgatgacgcg gagtggatgg
     1801 ccaagcaggt ttaccttaat gtaggtaact tcctgttggg ggtcgccgcg ttggggctgg
     1861 acgctgtgcc tattgaaggt tttgacgccg ccattttgga tgcagagttt ggactcaaag
     1921 aaaaaggcta cactagcctc gtggtggttc cggtcggtca ccactcggtg gaggacttta
     1981 acgcgaccct ccccaagtcg cggctgccgc agaatatcac actcacggaa gtggctggtt
     2041 ctagtgcagg tcaaggtgca caggctggtc aaggagcaca ggctggttct agtgcaggta
     2101 tggatatcat ctcggtggca ttgaaacgtc attctaccaa agccttcgat gcgtcgaaaa
     2161 agcttacgcc agagcaagct gaacagatca agacgttgct tcagtattca cccagttcaa
     2221 ctaattccca accctggcac ttcatcgtgg cgtccacgga ggaagggaaa gcgcgagtag
     2281 cgaaatcagc agccggcaac tatgtcttta acgaacgcaa aatgctcgat gcaagccacg
     2341 tggtggtctt ttgtgccaaa accgcaatgg acgatgtatg gcttaagctg gtagtggacc
     2401 aagaggatgc ggatggaagg ttcgctacac cggaggcgaa agccgcgaat gataagggaa
     2461 gaaagttctG Ggcagacatg catcgaaaag acctccatga tgacgcggag tggatggcca
     2521 agcaggttta ccttaatgta ggtaacttcc tgttgggggt cgccgcgttg gggctggacg
     2581 ctgtgcctat tgaaggtttt gacgccgcca ttttggatgc agagtttgga ctcaaagaaa
     2641 aaggctacac tagcctcgtg gtggttccgg tcggtcacca ctcggtggag gactttaacg
     2701 cgaccctccc caagtcgcgg ctgccgcaga atatcacact cacggaagtg gctggttcta
     2761 gtgcaggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     2821 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2881 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2941 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     3001 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     3061 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     3121 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     3181 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     3241 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     3301 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     3361 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     3421 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggctg
     3481 gttctagtCT CGAGtaaGAT atcaccggtA CGCGTtgatc aGCTAGCACT AGTATAACTT
     3541 CGTATAGCAT ACATTATACG AAGTTATGtG TCGATGGTGA TGCTTGGCAA TTCGGGTCGA
     3601 TGGTGAAGCA TTGGTCTTCC TATTTTGATA TAACTTCGTA TAGGATACTT TATACGAAGT
     3661 TATGGGGATC ATcacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg
     3721 cctatcagaa acgcaagagt cttctctgtc tcgacaagcc cagtttctat tggtctcctt
     3781 aaacctgtct tgtaaccttg atacttaccT GATCCaacgg ctgcgacttg gggcaggggg
     3841 tcctaggggt ggtctggcca ggagccgcgg gggcggactg cgcggtgccc aggagcacag
     3901 ctgcgctctc aggcacgaca cgactcctcc gctgcccacc gcagactgag gcagcgctga
     3961 gtcgccggcg ccgcagcgca gatggtcgcg cccgtgcccc cctatctcgc gcctcgcgtg
     4021 gtgcggtccg gctgggccgg cggcggcgcg gacgcgacca aggtggccgg gaaggggagt
     4081 ttgcggggga ccggcgagtg acgtcagcgc gccttcagtg ctgaggcggc ggtggcgcgc
     4141 gccgccaggc gggggcgaag gcactgtccg cggtgctgaa gctggcagtg cgcacgcgcc
     4201 tcgccgcatc ctgtttcccc tccccctctc tgatagggga tgcgcaattt ggggaatggg
     4261 ggttgggtgc ttgtccagtg ggtcggggtc ggtcgtcaat ttaaatactg aaAAGGGCGA
     4321 ATTCCAGCAC ACTGGAGCTC GGatcttcct agagcatggc tacgtaGATA AGTAGCATGG
     4381 CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG
     4441 CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG
     4501 GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
     4561 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC
     4621 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
     4681 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
     4741 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA
     4801 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
     4861 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG
     4921 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
     4981 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT
     5041 ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
     5101 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
     5161 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT
     5221 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA
     5281 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC
     5341 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA
     5401 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC
     5461 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT
     5521 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT
     5581 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC
     5641 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA
     5701 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
     5761 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
     5821 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT
     5881 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT
     5941 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA
     6001 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
     6061 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG
     6121 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC
     6181 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
     6241 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
     6301 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
     6361 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
     6421 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
     6481 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
     6541 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
     6601 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
     6661 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
     6721 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
     6781 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
     6841 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
     6901 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
     6961 AGCGAGTCAG TGAGCGAGGA AGCGGAAG
//

