LOCUS Generic_Cas9_Don 6440 bp ds-DNA linear 04-OCT-2016 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >Cherry in bluescript COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 4977..5765 /label=NeoR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4623..4891 /label=SV40 promoter /ApEinfo_fwdcolor=#ff344a /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2618..2623 /label=ClaI /ApEinfo_fwdcolor=#ff1b04 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6102..6115 /label=M13R /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2636..2641 /label=EcoRI /ApEinfo_fwdcolor=#ff1b04 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3629..3745 /label=mClover /ApEinfo_fwdcolor=#2eff28 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2630..2635 /label=EcoRV /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3595..3628 /label=Loxp1 /ApEinfo_fwdcolor=#201eff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2624..2629 /label=HindIII /ApEinfo_fwdcolor=#f7ff58 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3759..3987 /label=SV40 PolyA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2588..2593 /label=Kpn! /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2660..2679 /label=AID hygro F /ApEinfo_fwdcolor=#fffc1b /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3629..6115 /label=PCR pieve for generic targeting vector /ApEinfo_fwdcolor=#f0ff2c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2609..2614 /label=Sall1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6116..6149 /label=loxp /ApEinfo_fwdcolor=#5cffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3587..3594 /label=NotI /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3587..3594 /label=New Feature /ApEinfo_fwdcolor=#2a3dff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3575..3580 /label=SpeI /ApEinfo_fwdcolor=#255bff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2516..2536 /label=generic cas ins up seq /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6150..6157 /label=NotI(1) /ApEinfo_label=NotI /ApEinfo_fwdcolor=#162aff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2642..2659 /label=GS3 linker /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2672..2857 /label=mAID /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2867..3574 /label=mCherry /ApEinfo_fwdcolor=#ff1332 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6168..6173 /label=SacI /ApEinfo_fwdcolor=#fffd1f /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 61 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 121 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 181 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 241 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 301 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA 361 ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC 421 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG 481 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC 541 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT 601 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT 661 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC 721 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT 781 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA 841 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA 901 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT 961 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA 1021 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG 1081 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT 1141 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT 1201 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC 1261 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA 1321 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC 1381 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG 1441 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC 1501 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 1561 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 1621 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 1681 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 1741 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 1801 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 1861 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 1921 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 1981 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 2041 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 2101 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 2161 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC 2221 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC 2281 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT 2341 CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC 2401 GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT 2461 ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC 2521 AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA 2581 AAAGCTGGGT ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT 2641 Cggtagcggc agcggtagcA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA 2701 GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG 2761 CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC 2821 ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAAgct agcatggtga gcaagggcga 2881 ggagGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC 2941 CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC 3001 CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT 3061 GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA 3121 CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAATTGGGAG CGCGTGATGA ACTTCGAGGA 3181 CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA 3241 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGT GTCGTACCAT 3301 GGGCTGGGAG GCCTCCACTG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT 3361 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA 3421 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCGACATCA AGTTGGACAT 3481 CCTTTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA 3541 CTCCACCGGC Ggcatggacg agctgtacaa gtaaACTAGT TCTAGAGCGG CCGCATAACT 3601 TCGTATAATG TATGCTATAC GAAGTTATta cctgagccat cagtccAAGc tgagcaaaga 3661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggattac 3721 acatggcatg gacgagctgt acaagtaatc tagataactg atcataatca gccataccac 3781 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 3841 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 3901 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 3961 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa 4021 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 4081 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 4141 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 4201 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 4261 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 4321 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 4381 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 4441 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 4501 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 4561 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt 4621 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 4681 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 4741 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 4801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 4861 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 4921 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg 4981 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 5041 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 5101 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 5161 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 5221 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 5281 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 5341 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 5401 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 5461 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 5521 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 5581 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 5641 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 5701 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 5761 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 5821 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 5881 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 5941 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac 6001 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg ttGGATCCGG TACccagctt 6061 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgATAAC 6121 TTCGTATAGC ATACATTATA CGAAGTTATG CGGCCGCCAC CGCGGTGGAG CTCCAATTCG 6181 CCCTATAGTG AGTCGTATTA CGCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG 6241 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG 6301 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 6361 GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 6421 GTGACCGCTA CACTTGCCAG //