LOCUS       Exported               14704 bp ds-DNA     circular SYN 06-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   MMCTLab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, July 6, 2018 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             94..327
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    399..520
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      560..695
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(716..734)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(744..760)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      902..1357
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1383..1487
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1488..2348
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2519..3107
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3395..3416
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3431..3461
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3469..3485
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3493..3509
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3530..3548
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             3803..3983
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4030..4155
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4648..4881
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5377..5493
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5550..5574
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        5602..5981
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5982..6185
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        6230..6248
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6290..7009
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    7050..7070
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB5"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7057..8392
                     /note="Sequenced region July 2016 I+II"
                     /note="Sequencing II: only good ending region taken into 
                     account, as then sequencing errors 2016-07-29 JM"
     misc_feature    7104..7114
                     /note="TCF binding site"
     misc_feature    7119..7129
                     /note="TCF binding site"
     misc_feature    7134..7144
                     /note="TCF binding site"
     misc_feature    7150..7160
                     /note="TCF binding site"
     misc_feature    7165..7175
                     /note="TCF binding site"
     misc_feature    7180..7190
                     /note="TCF binding site"
     misc_feature    7195..7205
                     /note="TCF binding site"
     promoter        7245..7276
                     /note="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             7338..7339
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation=""
     CDS             7350..8300
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /note="iRFP713"
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     protein_bind    complement(8386..8406)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB4"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        8446..9783
                     /note="hPTCH1-wt promoter"
                     /note="Sequenced by JM, 3.5.2016"
     protein_bind    8528..8536
                     /bound_moiety="Gli1"
                     /note="BS1 (GACCTCCCA)"
     misc_binding    complement(8847..8855)
                     /bound_moiety="Gli1"
                     /note="BS2 (rev: GACCACCCA)"
     CDS             9806..10519
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /note="mTurquoise2"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     protein_bind    10702..10722
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB3"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        10732..10738
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    10801..10806
                     /note="CBF1 binding site"
                     /note="Presumptive first CBF binding site GTGGGAA"
     misc_feature    10836..10842
                     /note="CBF1 binding site"
     misc_feature    10871..10877
                     /note="CBF1 binding site"
     misc_feature    10906..10912
                     /note="CBF1 binding site"
     promoter        10927..11123
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      10974..11109
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             11205..12635
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /note="tdTomato"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(12656..12672)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(12684..12708)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    12739..13327
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(13210..13221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     promoter        13341..13840
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             13882..14676
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 cgcgtatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
       61 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
      121 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
      181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      301 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
      361 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
      421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
      541 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      601 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      661 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
      721 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      781 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      841 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      901 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      961 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1021 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1081 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1141 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     1201 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     1261 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     1321 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     1381 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1441 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1501 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1561 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1621 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1681 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1741 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1801 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1861 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1921 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1981 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2041 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2101 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2161 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2221 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2281 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2341 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2401 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2461 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2521 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2581 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2641 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2701 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2761 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2821 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2881 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2941 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3001 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3061 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3121 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3181 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3241 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3301 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3361 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     3421 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     3481 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     3541 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     3601 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     3661 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     3721 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     3781 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     3841 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     3901 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     3961 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     4021 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     4081 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     4141 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     4201 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     4261 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     4321 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     4381 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     4441 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     4501 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     4561 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     4621 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     4681 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     4741 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     4801 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     4861 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     4921 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     4981 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     5041 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     5101 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     5161 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     5221 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     5281 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     5341 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     5401 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     5461 attacaaaaa caaattacaa aattcaaaat tttatcgtcc agtgtggtgg aattgagtat
     5521 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctgcgatg
     5581 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     5641 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     5701 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     5761 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     5821 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     5881 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     5941 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     6001 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     6061 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     6121 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     6181 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     6241 actataggga gacccaagct tggtaccgag ctcggatcct aaagccacca tggtgagcaa
     6301 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6361 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6421 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6481 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6541 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6601 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6661 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6721 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6781 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6841 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6901 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6961 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga
     7021 ctctagagct cgagcatgca tctagaggac aactttgtat acaaaagttg ggagacccaa
     7081 gcttggtacc gagctcttac gcgagatcaa agggggtaag atcaaagggg gtaagatcaa
     7141 aggggcgcga gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg ggtaagatca
     7201 aaggggcgcg cccgcgtgct agcccgggct cgagatctag actctagagg gtatataatg
     7261 gaagctcgaa ttccagcttg gcattccggt actgttggta aaaagcttgg cattccggta
     7321 ctgttggtaa agccaccatc agctgcacca tggcggaagg atccgtcgcc aggcagcctg
     7381 acctcttgac ctgcgacgat gagccgatcc atatccccgg tgccatccaa ccgcatggac
     7441 tgctgctcgc cctcgccgcc gacatgacga tcgttgccgg cagcgacaac cttcccgaac
     7501 tcaccggact ggcgatcggc gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg
     7561 agacgcacaa ccgtctgacg atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga
     7621 tcactgtcgg cttcacgatg cgaaaggacg caggcttcat cggctcctgg catcgccatg
     7681 atcagctcat cttcctcgag ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt
     7741 tcttccgccg caccaacagc gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg
     7801 cctgcgccgc cgcggcgcaa gaggtgcgga agattaccgg cttcgatcgg gtgatgatct
     7861 atcgcttcgc ctccgacttc agcggcgaag tgatcgcaga ggatcggtgc gccgaggtcg
     7921 agtcaaaact aggcctgcac tatcctgcct caaccgtgcc ggcgcaggcc cgtcggctct
     7981 ataccatcaa cccggtacgg atcattcccg atatcaatta tcggccggtg ccggtcaccc
     8041 cagacctcaa tccggtcacc gggcggccga ttgatcttag cttcgccatc ctgcgcagcg
     8101 tctcgcccgt ccatctggaa ttcatgcgca acataggcat gcacggcacg atgtcgatct
     8161 cgattttgcg cggcgagcga ctgtggggat tgatcgtttg ccatcaccga acgccgtact
     8221 acgtcgatct cgatggccgc caagcctgcg agctagtcgc ccaggttctg gcctggcaga
     8281 tcggcgtgat ggaagagtga gtcactagta acggccgcca gtgtgctgga attctgcaga
     8341 tatccatcac actggcggcc gctcgagcat gcatctagag gcacccaact tttctataca
     8401 aagttgggag acccaagctt ggtaccgagc tcttacgcgt gctagagctt gcatgcctgc
     8461 aggtcgacac actggcgcac tatccagcac gctgtcatta ttggtgcagg cctgggaatg
     8521 cgagccggac ctcccagtat ttgctgcagc ttgaggccaa ggtgcctctg aaaggctttt
     8581 ctttgaggaa tttcagaaat tgtttaagcg cttaaaaaaa tattacattt ccccccgtgt
     8641 ctaccgcgag gcaaatgttc gtgtgtgtgc gtgcgcgcgc gtgtgtgcgc gcaaaggcat
     8701 cccacaaaaa gaagacactg ttgaaaagaa aaggaataag agagaaagga gggggagcat
     8761 tccttaatgg aagtattgca tgcgagaagg ttggcgctct cagaggctgc cccgaagccg
     8821 aggatgcaca cactgggttg cctacctggg tggtctctct actttggtga gctgttgcta
     8881 agcagctaat aatagtcatg tttgctgagt aatttctgcc ctccgcgagc caatcgcgtc
     8941 gcagaaaccc aagccatctg acagccccgg gggcgggagc tccagccttt ttctctttcg
     9001 ctcgctctct ccccctccac cccctcccct cccctcctcc tcttctttct ccaaatggag
     9061 agagttcttt tttttcttct tccatctcat ctattgaatc aaggagctgc tgcggccgct
     9121 gcccgctgca cacacacaga gcggagtccc caggtcccgg gagcgagaga ggcgcgctgc
     9181 acggggacag aatggtccag cgggttgccg aggagcacaa gaaagcagag tccgggaccg
     9241 agcagccacc gcgaacccag cagccagagc ccgagcagcc cgagcagcag ctcctggagc
     9301 cgccaccgcc agcagcagcc accgcgggag cagcggcagc tgcggctgcc cgggcccggc
     9361 agcgctgaga cccgcagggc accctcggac cccgcggcgg cggcggctct gcgcttgccc
     9421 tccgcggccg ctcgggcgac ccgggaggcg ccgagagagc cagcgcggcc ggcgggagca
     9481 gcggggattc gctggctctt tctgcagtga aggggtcgcg gcgcggggcg gggggcgggt
     9541 agggggagtt ggggaccgca aggagtgccg cggaagcgcc cgaaggacag gctcgctcgg
     9601 cgcgccggct ctcgctcttc cgcgaactgg atgtgggcag cggcggccgc agagacctcg
     9661 ggacccccgc gcaatgtggc aatggaaggc gcagggtctg actccccggc agcggccgcg
     9721 gccgcagcgg cagcagcgcc cgccgtgtga gcagcagcag cggctggtct gtcaaccgga
     9781 gcggatctgc gatctaagta agcttatggt gagcaagggc gaggagctgt tcaccggggt
     9841 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     9901 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     9961 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt
    10021 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
    10081 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
    10141 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
    10201 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta
    10261 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
    10321 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
    10381 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
    10441 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
    10501 cggcatggac gagctgtaca gtgagctgca gaacaagctg gaagagttgg atctggactc
    10561 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggat
    10621 ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca ctggcggccg
    10681 ctcgagcatg catctagagg acaactttgt ataataaagt tgctgtggaa tgtgtgtcca
    10741 tatgataacc gtattaccgc catgcattag ttattaatcg agatctggtg taaacacgcc
    10801 gtgggaaaaa atttatggat ctggagtaac acgccgtggg aaaaaattta tggatctgga
    10861 gtaacacgcc gtgggaaaaa atttatggat ctggagtaac acgccgtggg aaaaaattta
    10921 tggatctgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    10981 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    11041 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    11101 ttggaggcct aggcttttgc aaaaagctgg ctagcgctac cggactcaga tctcgagctc
    11161 aagcttcgaa ttcgccacca tgccagagcc aggatcccgc caccatggtg agcaagggcg
    11221 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
    11281 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
    11341 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
    11401 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
    11461 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
    11521 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
    11581 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
    11641 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
    11701 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
    11761 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
    11821 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
    11881 tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca
    11941 acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
    12001 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
    12061 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
    12121 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
    12181 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
    12241 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
    12301 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
    12361 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
    12421 aggccctgaa gctgaaggac ggcggccgct acctggtgga gttcaagacc atctacatgg
    12481 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
    12541 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
    12601 tgttcctgta cggcatggac gagctgtaca agtaagaatt cgatatcaag cttatcgata
    12661 ccgtcgacct cgagcctcta gatacccagc tttcttgtac aaagtggttg atatccagca
    12721 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    12781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    12841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    12901 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    12961 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    13021 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    13081 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
    13141 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    13201 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    13261 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    13321 tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
    13381 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
    13441 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
    13501 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
    13561 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
    13621 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
    13681 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
    13741 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
    13801 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
    13861 acactcctgc aggcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
    13921 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    13981 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    14041 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
    14101 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    14161 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    14221 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    14281 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    14341 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    14401 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
    14461 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    14521 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    14581 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    14641 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
    14701 ccga
//
