LOCUS 887_pDONR_P2R-P3 4292 bp ss-DNA circular SYN 20-Jun-2011 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers source <734..>2407 /mol_type="other DNA" /note=" [Split]" /organism="synthetic DNA construct" misc_feature 58..121 /citation=[5] /note="L-strand Y effector site" primer_bind 537..552 /note="M13 F" misc_feature complement(537..552) /label=M13(-20) forward misc_recomb 591..>733 /note="attP2R recomb site" misc_feature 730..>733 /note="recombination area" misc_feature 734..755 /note="mlm 413" misc_feature 734..1098 /note="He1 promoter" frag 734..2407 /note="2926 to 4599 of 1372Tol2 5xUAS M-TagYFPgi--he1a M-EYFP" misc_feature 905..922 /note="high cons 1a 1b & 2" misc_feature 964..983 /note="high cons 1a 1b & 2" misc_feature 989..1010 /note="s.5XUAS_MYFP_F_S.06.17" misc_feature 995..1023 /note="high cons 1a 1b & 2" misc_feature 1018..1022 /note="TATA" misc_feature 1048..1098 /note="Exon 1 He1a (partial exon up to ATG)" misc_feature complement(1082..1104) /note="s.5XUAS-MYFP-F_S.06.11" misc_feature 1082..1104 /note="s.5XUAS_MYFP_F_S.06.14" misc_feature 1105..1113 /note="Kozak seq" misc_feature 1114..1173 /note="Gap43 dual palmitoylation seq" misc_feature 1174..1218 /note="linker" misc_feature complement(1200..1220) /note="a.5XUAS-MYFP-F_S.06.8" misc_feature 1219..1939 /note="EYFP marker" misc_feature 1222..1224 /note="Clontech mutation" misc_feature 1414..1416 /note="YFP mutation" misc_feature 1423..1425 /note="YFP mutation" misc_feature 1435..1437 /note="YFP mutation" primer_bind 1662..1681 /note="BK Primer 6" primer_bind 1814..1833 /note="BK Primer 7" misc_feature 1873..1890 /note="s.5XUAS-MYFP-F_S.06.7" misc_feature 1912..1914 /note="Clontech mutation" misc_feature complement(1916..1937) /note="a.5XUAS-MYFP-F_S.06.6" misc_feature 2179..2407 /note="BGH polyA seq" /note="BGH polyA seq (bovine growth hormone polyadenylation seq)" misc_feature complement(2188..2209) /note="s.5XUAS-MYFP-F_S.06.16" misc_feature 2388..2407 /note="mlm 414" misc_recomb <2408..2496 /note="attP3 recomb site" promoter complement(2533..2552) /note="T7" misc_feature 2533..2552 /label=T7 promoter primer_bind 2557..2573 /note="binding site of pUC" /note="/M13 reverse sequencing primer" misc_feature 2557..2573 /note="pUC;M13 reverse primer binding site" misc_feature 2686..3495 /note="Kanamycin" rep_origin 3642..4230 /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA" /note="/DNA switch point)" rep_origin complement(3642..4230) /note="pMB1 origin of replication (counterclockwise) (RNAII -35 to RNA" /note="/DNA switch point)" misc_RNA 3678..4230 /note="RNAII transcript (complementary strand)" misc_RNA complement(3681..3788) /note="RNAI transcript" rep_origin complement(4197..4233) /note="ColE1-derived origin of replication" rep_origin complement(4219..4248) /note="ColE1-derived plasmid replication origin" BASE COUNT 1088 A 1079 C 1082 G 1043 T 0 OTHER ORIGIN ? 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCTG CAGCTCTAGA GCTCGAATTC TACAGGTCAC 601 TAATACCATC TAAGTAGTTG ATTCATAGTG ACTGCATATG TTGTGTTTTA CAGTATTATG 661 TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT 721 CTCGTTCAAC TTTTCAACCA CTCCAGGCAT AGCTAGAGCC ACTGATGCAT CTATAGAGCT 781 GAAGTAAAGA TAGGGTTGAT GCAAATCGAT TTAGAATTTT CAATGTAGAA AAAACAAAAT 841 AAAAAGTCTA TTCACTTGAT GGATAGATTA GTCAAACAAA CATAGAGTAG GCACTTCCCT 901 TGACTCTGTA AACTTTTGGA TTTAACAGAT TGTGCACTCC TTTTAGTTAA ACACATTAGT 961 CCCTGAAAAC TAAGACCCTA ACCATAATCC CATTGTAGAC CCACCTACTC GGGTGAATAT 1021 AAAGCTCGCA CAGATCATCT CACAGACATC AGACCTTGGC AAAGGAGCAT CCTGAACATC 1081 TCTACACACT GAGGCAACCC TAGGGCCGCC ACCATGCTGT GTTGTATCAG AAGAACAAAG 1141 CCTGTGGAGA AGAATGAGGA GGCTGACCAG GAACTGCAGA GCACGGTACC TAGAGCTAGA 1201 GACCCTCCTG TGGCTACAAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC 1261 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC 1321 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG 1381 CCCGTGCCCT GGCCCACCCT CGTGACCACC TTCGGCTACG GCCTGCAGTG CTTCGCCCGC 1441 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC 1501 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG 1561 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC 1621 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG 1681 GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC 1741 GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG 1801 CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG 1861 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG 1921 GACGAGCTGT ATAAGTGAGA ATTCTGCAGG AATTGGGTGG CATCCCTGTG ACCCCTCCCC 1981 AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA 2041 ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG 2101 GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCACGAG GGGGGGCCCG 2161 AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT 2221 CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG 2281 AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC 2341 AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAAGGC ATGCTGGGGA TGCGGTGGGC 2401 TCTATGGATT ATACAAAGTT GGCATTATAA AAAAGCATTG CTTATCAATT TGTTGCAACG 2461 AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGGAG CTCCATGGTA GCGTTAACGC 2521 GGCCGCGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC 2581 TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGA 2641 ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA 2701 CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA 2761 TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC 2821 GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT 2881 GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT 2941 ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC 3001 CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC 3061 CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT 3121 CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT 3181 GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA 3241 TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC 3301 GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG 3361 GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT 3421 TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC 3481 GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG 3541 ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC 3601 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 3661 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 3721 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 3781 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 3841 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 3901 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 3961 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 4021 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 4081 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 4141 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 4201 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 4261 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //