LOCUS       887_pDONR_P2R-P3        4292 bp ss-DNA     circular SYN 20-Jun-2011
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     source          <734..>2407
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="synthetic DNA construct"
     misc_feature    58..121
                     /citation=[5]
                     /note="L-strand Y effector site"
     primer_bind     537..552
                     /note="M13 F"
     misc_feature    complement(537..552)
                     /label=M13(-20) forward
     misc_recomb     591..>733
                     /note="attP2R recomb site"
     misc_feature    730..>733
                     /note="recombination area"
     misc_feature    734..755
                     /note="mlm 413"
     misc_feature    734..1098
                     /note="He1 promoter"
     frag            734..2407
                     /note="2926 to 4599 of 1372Tol2 5xUAS M-TagYFPgi--he1a
                     M-EYFP"
     misc_feature    905..922
                     /note="high cons 1a 1b & 2"
     misc_feature    964..983
                     /note="high cons 1a 1b & 2"
     misc_feature    989..1010
                     /note="s.5XUAS_MYFP_F_S.06.17"
     misc_feature    995..1023
                     /note="high cons 1a 1b & 2"
     misc_feature    1018..1022
                     /note="TATA"
     misc_feature    1048..1098
                     /note="Exon 1 He1a (partial exon up to ATG)"
     misc_feature    complement(1082..1104)
                     /note="s.5XUAS-MYFP-F_S.06.11"
     misc_feature    1082..1104
                     /note="s.5XUAS_MYFP_F_S.06.14"
     misc_feature    1105..1113
                     /note="Kozak seq"
     misc_feature    1114..1173
                     /note="Gap43 dual palmitoylation seq"
     misc_feature    1174..1218
                     /note="linker"
     misc_feature    complement(1200..1220)
                     /note="a.5XUAS-MYFP-F_S.06.8"
     misc_feature    1219..1939
                     /note="EYFP marker"
     misc_feature    1222..1224
                     /note="Clontech mutation"
     misc_feature    1414..1416
                     /note="YFP mutation"
     misc_feature    1423..1425
                     /note="YFP mutation"
     misc_feature    1435..1437
                     /note="YFP mutation"
     primer_bind     1662..1681
                     /note="BK Primer 6"
     primer_bind     1814..1833
                     /note="BK Primer 7"
     misc_feature    1873..1890
                     /note="s.5XUAS-MYFP-F_S.06.7"
     misc_feature    1912..1914
                     /note="Clontech mutation"
     misc_feature    complement(1916..1937)
                     /note="a.5XUAS-MYFP-F_S.06.6"
     misc_feature    2179..2407
                     /note="BGH polyA seq"
                     /note="BGH polyA seq (bovine growth hormone
                     polyadenylation seq)"
     misc_feature    complement(2188..2209)
                     /note="s.5XUAS-MYFP-F_S.06.16"
     misc_feature    2388..2407
                     /note="mlm 414"
     misc_recomb     <2408..2496
                     /note="attP3 recomb site"
     promoter        complement(2533..2552)
                     /note="T7"
     misc_feature    2533..2552
                     /label=T7 promoter
     primer_bind     2557..2573
                     /note="binding site of pUC"
                     /note="/M13 reverse sequencing primer"
     misc_feature    2557..2573
                     /note="pUC;M13 reverse primer binding site"
     misc_feature    2686..3495
                     /note="Kanamycin"
     rep_origin      3642..4230
                     /note="pMB1 origin of replication (clockwise) (RNAII -35
                     to RNA"
                     /note="/DNA switch point)"
     rep_origin      complement(3642..4230)
                     /note="pMB1 origin of replication (counterclockwise)
                     (RNAII -35 to RNA"
                     /note="/DNA switch point)"
     misc_RNA        3678..4230
                     /note="RNAII transcript (complementary strand)"
     misc_RNA        complement(3681..3788)
                     /note="RNAI transcript"
     rep_origin      complement(4197..4233)
                     /note="ColE1-derived origin of replication"
     rep_origin      complement(4219..4248)
                     /note="ColE1-derived plasmid replication origin"
BASE COUNT      1088 A      1079 C      1082 G      1043 T         0 OTHER
ORIGIN      ?
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCTG CAGCTCTAGA GCTCGAATTC TACAGGTCAC
      601 TAATACCATC TAAGTAGTTG ATTCATAGTG ACTGCATATG TTGTGTTTTA CAGTATTATG
      661 TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT
      721 CTCGTTCAAC TTTTCAACCA CTCCAGGCAT AGCTAGAGCC ACTGATGCAT CTATAGAGCT
      781 GAAGTAAAGA TAGGGTTGAT GCAAATCGAT TTAGAATTTT CAATGTAGAA AAAACAAAAT
      841 AAAAAGTCTA TTCACTTGAT GGATAGATTA GTCAAACAAA CATAGAGTAG GCACTTCCCT
      901 TGACTCTGTA AACTTTTGGA TTTAACAGAT TGTGCACTCC TTTTAGTTAA ACACATTAGT
      961 CCCTGAAAAC TAAGACCCTA ACCATAATCC CATTGTAGAC CCACCTACTC GGGTGAATAT
     1021 AAAGCTCGCA CAGATCATCT CACAGACATC AGACCTTGGC AAAGGAGCAT CCTGAACATC
     1081 TCTACACACT GAGGCAACCC TAGGGCCGCC ACCATGCTGT GTTGTATCAG AAGAACAAAG
     1141 CCTGTGGAGA AGAATGAGGA GGCTGACCAG GAACTGCAGA GCACGGTACC TAGAGCTAGA
     1201 GACCCTCCTG TGGCTACAAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC
     1261 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC
     1321 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG
     1381 CCCGTGCCCT GGCCCACCCT CGTGACCACC TTCGGCTACG GCCTGCAGTG CTTCGCCCGC
     1441 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC
     1501 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG
     1561 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC
     1621 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG
     1681 GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC
     1741 GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
     1801 CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG
     1861 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG
     1921 GACGAGCTGT ATAAGTGAGA ATTCTGCAGG AATTGGGTGG CATCCCTGTG ACCCCTCCCC
     1981 AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA
     2041 ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG
     2101 GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCACGAG GGGGGGCCCG
     2161 AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT
     2221 CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG
     2281 AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC
     2341 AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAAGGC ATGCTGGGGA TGCGGTGGGC
     2401 TCTATGGATT ATACAAAGTT GGCATTATAA AAAAGCATTG CTTATCAATT TGTTGCAACG
     2461 AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGGAG CTCCATGGTA GCGTTAACGC
     2521 GGCCGCGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC
     2581 TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGA
     2641 ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA
     2701 CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA
     2761 TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC
     2821 GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT
     2881 GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT
     2941 ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC
     3001 CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC
     3061 CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT
     3121 CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT
     3181 GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA
     3241 TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC
     3301 GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG
     3361 GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT
     3421 TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC
     3481 GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG
     3541 ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC
     3601 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG
     3661 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
     3721 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
     3781 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
     3841 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
     3901 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
     3961 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
     4021 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
     4081 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC
     4141 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
     4201 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
     4261 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT
//
