LOCUS       491_p3E-polyA           4439 bp ss-DNA     circular SYN 19-Nov-2012
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     source          748..>1112
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="recombinant plasmid"
     misc_feature    537..552
                     /note="M13F"
     misc_feature    591..747
                     /note="attR2"
     misc_feature    748..>1112
                     /note="Sequenced ZK 1/28/2015 [Split]"
     frag            748..>1112
                     /date="05-Oct-2015"
                     /note="3420 to 5168 of 1368pTol2 5xUAS
                     mRFP670N-NTRmut-HE-mCherry [Split]"
     misc_feature    748..1112
                     /note="He1 promoter"
     frag            748..1112
                     /date="05-Oct-2015"
                     /note="748 to 1112 of HE_mCherry_p3E"
     misc_feature    919..936
                     /note="high cons 1a 1b & 2"
     misc_feature    978..997
                     /note="high cons 1a 1b & 2"
     misc_feature    1009..1037
                     /note="high cons 1a 1b & 2"
     misc_feature    1032..1036
                     /note="TATA"
     misc_feature    1062..1112
                     /note="Exon 1 He1a (partial exon up to ATG)"
     frag            <1113..2546
                     /date="05-Oct-2015"
                     /note="3079 to 4804 of 1370Tol2 5xUAS-MCFPgi--He-CFP"
     misc_feature    1119..1127
                     /note="Kozak seq"
     misc_feature    1128..1187
                     /note="Gap43 dual palmitoylation seq"
     misc_feature    1188..1203
                     /note="linker"
     misc_feature    1233..1952
                     /note="CFP"
     primer_bind     complement(1919..1950)
                     /note="BsrgI-CFP (3' primer)"
     misc_feature    2318..2546
                     /note="BGH polyA seq"
                     /note="BGH polyA seq (bovine growth hormone
                     polyadenylation seq)"
     misc_feature    2548..2643
                     /note="attL3"
     misc_feature    2677..2696
                     /note="T7"
     misc_feature    2833..3642
                     /note="kanR"
BASE COUNT      1128 A      1126 C      1108 G      1077 T         0 OTHER
ORIGIN      ?
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCTG CAGCTCTAGA GCTCGAATTC TACAGGTCAC
      601 TAATACCATC TAAGTAGTTG GTTCATAGTG ACTGCATATG TTGTGTTTTA CAGTATTATG
      661 TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT
      721 CTCGTTCAAC TTTCTTGTAC AAAGTGGTCA ACCACTCCAG GCATAGCTAG AGCCACTGAT
      781 GCATCTATAG AGCTGAAGTA AAGATAGGGT TGATGCAAAT CGATTTAGAA TTTTCAATGT
      841 AGAAAAAACA AAATAAAAAG TCTATTCACT TGATGGATAG ATTAGTCAAA CAAACATAGA
      901 GTAGGCACTT CCCTTGACTC TGTAAACTTT TGGATTTAAC AGATTGTGCA CTCCTTTTAG
      961 TTAAACACAT TAGTCCCTGA AAACTAAGAC CCTAACCATA ATCCCATTGT AGACCCACCT
     1021 ACTCGGGTGA ATATAAAGCT CGCACAGATC ATCTCACAGA CATCAGACCT TGGCAAAGGA
     1081 GCATCCTGAA CATCTCTACA CACTGAGGCA ACCCTAGGGC CGCCACCATG CTGTGTTGTA
     1141 TCAGAAGAAC AAAGCCTGTG GAGAAGAATG AGGAGGCTGA CCAGGAACTG CAGAGCACGG
     1201 TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
     1261 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
     1321 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA
     1381 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TGGGGCGTGC
     1441 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
     1501 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
     1561 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
     1621 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC ATCAGCCACA
     1681 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC
     1741 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG
     1801 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA
     1861 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA
     1921 TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG CGACTCTAGA TCATAATCAG
     1981 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA
     2041 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTATCGAA TTCTGCAGGA ATTGGGTGGC
     2101 ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA
     2161 CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA
     2221 ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA
     2281 GGGCACGAGG GGGGGCCCGA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG
     2341 CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT
     2401 GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT
     2461 CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAAGGCA
     2521 TGCTGGGGAT GCGGTGGGCT CTATGGCCAA CTTTATTATA CAAAGTTGGC ATTATAAAAA
     2581 AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA
     2641 TTTGGAGCTC CATGGTAGCG TTAACGCGGC CGCGATATCC CCTATAGTGA GTCGTATTAC
     2701 ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT
     2761 GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT
     2821 ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC
     2881 ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG
     2941 ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA
     3001 GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT
     3061 ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC
     3121 ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA
     3181 AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT
     3241 TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC
     3301 GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC
     3361 TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT
     3421 TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA
     3481 CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG
     3541 TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG
     3601 AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG
     3661 TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA
     3721 TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
     3781 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
     3841 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA
     3901 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC
     3961 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA
     4021 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
     4081 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
     4141 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT
     4201 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC
     4261 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC
     4321 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
     4381 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT
//
