LOCUS       negMaLionB/pcDNA3.1(-)        1320 bp    DNA     linear       11-JUN-2018
SOURCE      
  ORGANISM  
COMMENT     
            
            ApEinfo:methylated:1
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12328854|
COMMENT     VNTDBDATE|-12328854|
COMMENT     LSOWNER|
COMMENT     VNTNAME|negMaLionB/pcDNA3.1(-)|
COMMENT     VNTAUTHORNAME|Tetsuya Kitaguchi|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    57..138
                     /invitrogen="1620000"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=MCS
     primer          25..44
                     /invitrogen="2100000"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="27"
                     /label=T7\primer
     promoter        25..41
                     /invitrogen="2370000"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    1285..1294
                     /invitrogen="1620000"
                     /ApEinfo_label=MCS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=MCS(1)
BASE COUNT      304 a       431 c       396 g       189 t 
ORIGIN
        1 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta 
       61 gcgtttaaac gggccctcta gactcgagcg gccgccactg tgctggatat ctgcagaatt 
      121 ccaccacact ggactagtgg atccatggtg agcaagggcg aggagctgtt caccggggtg 
      181 gtgcccatcc aggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 
      241 gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 
      301 aagctgcccg tgccctggcc caccctcgtg accaccctgt cacacggcgt gcagtgcttc 
      361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccggaggc 
      421 tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 
      481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 
      541 gaggacggca acatcctggg gcacaagctg gagtacaacc tccgcaatat gaagaccgtg 
      601 aaggtgaaca tcaccacccc cgacggcccc gtgtacgacg ccgacatcga gatggtgagc 
      661 gtgcgggccg agagcggcga cctgggcatc ctgcccggcc acatccccac caaggccccc 
      721 ctgaagatcg gcgccgtgcg gctgaagaag gacggccaga ccgagtacgt ggccgtgagc 
      781 ggcggcaacg tggaggtgcg gcccgaccag gtgaccatca ccgcccaggc cgccgagacc 
      841 gccgagggca tcgacaagga gcgggccgag gccgcccggc agcgggccca ggagcggctg 
      901 aacagccaga gcgacgacac cgacatccgg cgggccgagc tggccctgca gcgggccctg 
      961 aaccggctgg acgtggccga gatgaaggca gaattctcac cagagaacgt ctatatcaag 
     1021 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgagggc 
     1081 ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg 
     1141 ctgctgcccg acaaccacta cctgagcgtg cagtccatac tttcgaaaga ccccaacgag 
     1201 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 
     1261 gacgagctgt acaagtaaaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc 
//
