LOCUS       pCDNA3.1-H-Luc_n        7047 bp ds-DNA     circular SYN 09-JUL-2018
DEFINITION  pCDNA3.1-H-Luc_nucl.
ACCESSION   New
KEYWORDS    pCDNA3.1-Halo[156(nLucCP67-68)158]-NLSx3.
SOURCE      
  ORGANISM  .
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 13, 2017 from SnapGene 2.0.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Synthetic"
     promoter        863..881
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     misc_feature    914..955
                     /note="Geneious type: Tag"
                     /standard_name="V5 tag"
     gene            956..976
                     /note="Geneious type: Gene"
                     /standard_name="TEV"
     misc_feature    1008..1028
                     /note="Geneious type: attB1"
     CDS             1034..1042
                     /codon_start=1
                     /product="kozac(START)"
                     /note="kozac(START)"
                     /translation="TTM"
                     /standard_name="kozac(START)"
     CDS             1043..2479
                     /product="Halo[156(nLucCP67/68)158]"
                     /note="Halo[156(nLucCP67/68)158]"
                     /standard_name="H-Luc"
     CDS             2480..2596
                     /product="NLSx3"
                     /note="NLSx3"
                     /standard_name="NLSx3"
     misc_feature    2504..2593
                     /note="Geneious type: Localization"
                     /standard_name="NLS_tandem_repeat loc"
     misc_feature    complement(2601..2621)
                     /note="Geneious type: attB2"
     terminator      2644..2871
                     /note="Geneious type: Terminator"
                     /standard_name="bGH_PA term"
     misc_feature    2934..3240
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     misc_feature    complement(3348..3593)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="SV40ER reg"
     promoter        3492..3694
                     /note="Geneious type: Promoter"
                     /standard_name="SV40 prom"
     misc_feature    3539..3616
                     /note="Geneious type: Origin of Replication"
                     /standard_name="SV40 origin"
     misc_feature    3746..4546
                     /note="Geneious type: Marker"
                     /standard_name="NTP_II marker"
     promoter        complement(4942..4971)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    complement(5280..5896)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(6051..6911)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     promoter        complement(6953..6981)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttacc atgggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacggaaaa
      961 cctgtatttt cagggcccgg atccgtcgac gaattctgca gatatcacaa gtttgtacaa
     1021 aaaagcaggc ttcaccacca tgggatccga aatcggtact ggctttccat tcgaccccca
     1081 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
     1141 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
     1201 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
     1261 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
     1321 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
     1381 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
     1441 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
     1501 caccagcggc gaccaaatgg gccagatcga aaaaattttt aaggtggtgt accctgtgga
     1561 tgatcatcac tttaaggtga tcctgcacta tggcacactg gtaatcgacg gggttacgcc
     1621 gaacatgatc gactatttcg gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa
     1681 gatcactgta acagggaccc tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa
     1741 ccccgacggc tccctgctgt tccgagtaac catcaacgga gtgaccggct ggcggctgtg
     1801 cgaacgcatt ctggcgggtg gcaccggtgg ctctggcggt accggtggct ctatggtctt
     1861 cacactcgaa gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt
     1921 ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat
     1981 ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc
     2041 gtatgaaggt ctggacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg
     2101 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga
     2161 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat
     2221 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca
     2281 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga
     2341 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct
     2401 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc
     2461 tactctggag atttccggta agtccggact cagatctcga gctgatccaa aaaagaagag
     2521 aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaggatc
     2581 caccggatct agataggacc cagctttctt gtacaaagtg gtgataaacc cgctgatcag
     2641 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2701 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2761 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2821 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     2881 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     2941 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3001 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3061 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3121 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3181 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3241 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3301 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     3361 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3421 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3481 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3541 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3601 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     3661 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     3721 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     3781 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3841 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     3901 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     3961 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4021 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4081 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4141 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4201 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4261 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4321 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4381 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4441 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4501 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     4561 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     4621 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     4681 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     4741 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4801 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     4861 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4921 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4981 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5041 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5101 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5161 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5221 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5281 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5341 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5401 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5461 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5521 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5581 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5641 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5701 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5761 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5821 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7021 tttccccgaa aagtgccacc tgacgtc
//
