LOCUS       sg2M-sg3M+BB            3489 bp ds-DNA     circular     11-JUL-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2131..2222
                     /label="Modified scaffold"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2111..2130
                     /label="sg3 target"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(join(3011..3489,1..141))
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1793..2110
                     /label="U6 Promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2560..2579
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(2275..2293)
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1637..1656
                     /label="sg2 target"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      2498..2575
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1319..1636
                     /label="U6 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(296..1156)
                     /label="ORF frame 3"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2744..2763
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2292..2314)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2463..2642
                     /label="SV40_promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(1198..1226)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(2328..2357)
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      2656..2787
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            complement(296..1156)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1657..1748
                     /label="Modified scaffold"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
       61 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
      121 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
      181 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
      241 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
      301 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
      361 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
      421 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
      481 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
      541 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
      601 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
      661 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
      721 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
      781 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
      841 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
      901 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
      961 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
     1021 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
     1081 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
     1141 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
     1201 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
     1261 GCACATTTCC CCGAAAAGTG CCACCTGGTC GACATTGATT ATTGACTAGT AAGAATTCTG
     1321 TACAAAAAAG CAGGCTTTAA AGGAACCAAT TCAGTCGACT GGATCCGGTA CCAAGGTCGG
     1381 GCAGGAAGAG GGCCTATTTC CCATGATTCC TTCATATTTG CATATACGAT ACAAGGCTGT
     1441 TAGAGAGATA ATTAGAATTA ATTTGACTGT AAACACAAAG ATATTAGTAC AAAATACGTG
     1501 ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA AAATTATGTT TTAAAATGGA
     1561 CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
     1621 GAAAGGACGA AACACCGAAC CTCTACAAAT GTGGTAGTTT CAGAGCTATG CTGGAAACAG
     1681 CATAGCAAGT TGAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA CCGAGTCGGT
     1741 GCTTTTTTCT AGACCCAGCT TTCTTGTACA AAGTTGGCAT TATGCCCCCG GGTGTACAAA
     1801 AAAGCAGGCT TTAAAGGAAC CAATTCAGTC GACTGGATCC GGTACCAAGG TCGGGCAGGA
     1861 AGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG CTGTTAGAGA
     1921 GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA CGTGACGTAG
     1981 AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA TGGACTATCA
     2041 TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
     2101 ACGAAACACC GCAATGTATC TTATCATGTC GTTTCAGAGC TATGCTGGAA ACAGCATAGC
     2161 AAGTTGAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT
     2221 TTCTAGACCC AGCTTTCTTG TACAAAGTTG GCATTACGCC AAGCTTGGCG TAATCATGGT
     2281 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG
     2341 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT
     2401 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCGGATC CGCATCTCAA
     2461 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG
     2521 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC
     2581 CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
     2641 TTGCAAAAAG CTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
     2701 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
     2761 ATCAATGTAT CTTATCATGT CTGGATCCGC TGCATTAATG AATCGGCCAA CGCGCGGGGA
     2821 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG
     2881 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
     2941 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
     3001 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     3061 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     3121 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     3181 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC
     3241 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     3301 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     3361 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     3421 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA
     3481 TCTGCGCTC
//