LOCUS       Exported                2528 bp ds-DNA     circular SYN 29-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Bikard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2017 from SnapGene 4.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..45)
                     /label=LC515
                     /note="50-del-R2"
     primer_bind     complement(1..37)
                     /label=LC40
                     /note="ELZ2_1k_R"
     primer_bind     1..37
                     /label=LC41
                     /note="ELZ2mutBB_F"
     primer_bind     complement(1..20)
                     /label=LC288
                     /note="LacZ2mut1K_R"
     primer_bind     complement(3..23)
                     /label=LC936_pLC113_iR
     primer_bind     complement(3..21)
                     /label=LC316_sgRNA_R
     primer_bind     complement(20..39)
                     /label=LC325
                     /note="GFPint_R"
     primer_bind     20..37
                     /label=LC1312_pLC195_F_cos
     primer_bind     20..37
                     /label=LC914
                     /note="pLC108_BF"
     primer_bind     20..36
                     /label=LC11
                     /note="pZS24-BB-F"
     primer_bind     24..42
                     /label=ES58
                     /note="pACV5_primer2"
     primer_bind     26..44
                     /label=LC690
                     /note="pLC81_BB_F"
     primer_bind     26..44
                     /label=ACV3
                     /note="BB_pZS25_pTet_RecET_Fw"
     primer_bind     28..51
                     /label=LC374
                     /note="cos_R1"
     primer_bind     29..52
                     /label=LC584
                     /note="pLC64cos_R"
     primer_bind     complement(32..60)
                     /label=LC414
                     /note="NGS_P1_R"
     primer_bind     32..52
                     /label=ACV30
                     /note="pACV8_BB_F"
     primer_bind     32..52
                     /label=ACV24
                     /note="pACV6_BB_F"
     primer_bind     complement(33..52)
                     /label=LC20
                     /note="Ku-Seq7-R"
     primer_bind     complement(34..53)
                     /label=LC662
                     /note="pACV4_R"
     primer_bind     34..52
                     /label=LC380
                     /note="cos_R3"
     primer_bind     34..52
                     /label=LC387
                     /note="cos_R4"
     primer_bind     34..51
                     /label=LC527_pLC2_F1
     primer_bind     34..51
                     /label=LC318
                     /note="sgRNA_F2"
     primer_bind     34..51
                     /label=LC322
                     /note="sgRNABB2_F"
     primer_bind     39..58
                     /label=ES61
                     /note="pZA31luc_primer1"
     primer_bind     42..64
                     /label=LC645
                     /note="pLC51_BB_F"
     terminator      49..135
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     110..171
                     /label=LC74_CRRNAcos_F
     primer_bind     complement(113..135)
                     /label=SB18
                     /note="SB18_Intetrate49"
     primer_bind     114..135
                     /label=LC192
                     /note="pZA31BB_F"
     primer_bind     131..149
                     /label=LC671_Cos_F
     primer_bind     complement(131..149)
                     /label=LC191
                     /note="GFPmut2_R"
     misc_feature    150..373
                     /label=Minimal lambda cos sequence
     primer_bind     150..171
                     /label=LC90
                     /note="Cas9cos_F"
     primer_bind     complement(181..212)
                     /label=LC863
                     /note="new_NGS1R"
     primer_bind     complement(181..211)
                     /label=LC439_NGS_P1_R1
     primer_bind     complement(199..217)
                     /label=LC647
                     /note="cos_seq"
     primer_bind     225..240
                     /label=LC118
                     /note="treF_F"
     misc_feature    227..238
                     /label=Lambda cos site
     primer_bind     complement(235..250)
                     /label=B160
                     /note="rpsLsp-R"
     primer_bind     complement(353..413)
                     /label=LC75_CRRNAcos_R
     primer_bind     complement(353..373)
                     /label=LC91
                     /note="Cas9cos_R"
     primer_bind     376..392
                     /label=LC659
                     /note="sgRNAc_F3"
     primer_bind     381..394
                     /label=LC290
                     /note="lacZ2mut1kd_R"
     primer_bind     complement(396..416)
                     /label=LC234
                     /note="wtCas9BB_R2"
     primer_bind     complement(401..420)
                     /label=LC1313_pLC195_R_cos
     primer_bind     complement(401..420)
                     /label=LC668
                     /note="pLC79_F"
     primer_bind     complement(401..420)
                     /label=LC665
                     /note="pLC10_F2"
     primer_bind     complement(401..420)
                     /label=LC583
                     /note="pLC64cos_F"
     primer_bind     complement(401..420)
                     /label=B248
                     /note="VR"
     primer_bind     complement(401..419)
                     /label=LC331
                     /note="p4BB_F"
     primer_bind     complement(435..454)
                     /label=LC670_Cos_R
     primer_bind     complement(435..453)
                     /label=LC377
                     /note="cos_F2"
     primer_bind     complement(435..453)
                     /label=LC373
                     /note="Cos_F1"
     primer_bind     complement(435..453)
                     /label=LC220
                     /note="pCRRNA_R"
     primer_bind     436..453
                     /label=LC700
                     /note="pLC86insert_F"
     primer_bind     complement(443..467)
                     /label=oGR13
                     /note="Fse_SF_GFP_F"
     primer_bind     complement(461..479)
                     /label=LC369
                     /note="sgRNA_R4"
     primer_bind     complement(461..479)
                     /label=LC330
                     /note="crm_F"
     primer_bind     474..491
                     /label=LC99
                     /note="OriAspa_R"
     rep_origin      complement(520..1108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1016..1031)
                     /label=oGR256
                     /note="vector_F"
     primer_bind     1020..1039
                     /label=LC79
                     /note="ccdBspa_R"
     primer_bind     1020..1037
                     /label=oGR244
                     /note="seq_CAT_region_R"
     primer_bind     1118..1138
                     /label=BG36
                     /note="zeoR_R"
     primer_bind     1122..1139
                     /label=LC537
                     /note="smR_2"
     primer_bind     1126..1144
                     /label=LC644
                     /note="pLC51_BB_R"
     primer_bind     1126..1144
                     /label=LC589
                     /note="pLC65Smr_F"
     primer_bind     1126..1144
                     /label=LC528
                     /note="smR_R1_2"
     primer_bind     1126..1144
                     /label=LC526_smr_R1_1
     primer_bind     1130..1149
                     /label=LC233
                     /note="wtCas9BB_F2"
     primer_bind     complement(1135..1158)
                     /label=oGR336
                     /note="vector_pRG13A_F"
     primer_bind     1135..1151
                     /label=oGR255
                     /note="vector_R"
     primer_bind     1139..1157
                     /label=BG34
                     /note="specR_R"
     primer_bind     1139..1157
                     /label=oGR199
                     /note="R_AscI"
     primer_bind     1139..1157
                     /label=oGR163
                     /note="Em_85151_R1"
     primer_bind     1139..1157
                     /label=oGR155
                     /note="Em_82151_R1"
     primer_bind     1139..1157
                     /label=oGR161
                     /note="Em_84151_R1"
     primer_bind     1139..1157
                     /label=oGR159
                     /note="Em_83151_R1"
     primer_bind     1139..1157
                     /label=LC328
                     /note="SpecF"
     primer_bind     1139..1157
                     /label=LC218
                     /note="pCRRNA_F"
     primer_bind     1140..1159
                     /label=oGR348
                     /note="erm_gene_R"
     primer_bind     complement(1140..1159)
                     /label=LC235
                     /note="Orits_R1"
     primer_bind     1141..1158
                     /label=oGR337
                     /note="PCR_Kan_gene_R"
     terminator      1196..1290
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1291..1309)
                     /label=ACV19
                     /note="pACV2_R"
     misc_feature    1293..1322
                     /label=CBB CRISPR spapcer
                     /note="cut pELZ2mut1K backbone, cut pCRISPR, pZE21, pCRRNA 
                     backbone
                     "
     primer_bind     1293..1322
                     /label=LC131
                     /note="CBBELZ2_R"
     primer_bind     1302..1316
                     /label=LC545
                     /note="pLC45_R1"
     CDS             complement(1321..2115)
                     /codon_start=1
                     /label=kanR
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1325..1344)
                     /label=LC327
                     /note="sgRNABB3R"
     primer_bind     complement(1935..1954)
                     /label=ES2
                     /note="kan_R"
     primer_bind     2045..2064
                     /label=ES1
                     /note="kan_F"
     primer_bind     complement(2171..2182)
                     /label=V111
                     /note="pAV9_prefix_F"
     primer_bind     2199..2221
                     /label=B385
     primer_bind     2199..2220
                     /label=ACV17
                     /note="pACV2_F"
     primer_bind     2199..2220
                     /label=LC661
                     /note="pACV4_F"
     primer_bind     2206..2224
                     /label=LC890
                     /note="pLC105_IF1"
     primer_bind     2208..2230
                     /label=B377 pZ-F
                     /note="from pDB129 pZE21-CRISPR-bsaI"
     primer_bind     2210..2230
                     /label=LC368
                     /note="sgRNA_F4"
     primer_bind     complement(2215..2230)
                     /label=oGR338
                     /note="PCR_Kan_gene_F"
     primer_bind     complement(2232..2252)
                     /label=LC522_pLC2_R1
     primer_bind     complement(2232..2251)
                     /label=LC13
                     /note="pZS24-BB-R"
     primer_bind     2232..2245
                     /label=LC395
                     /note="PA-LigD_R"
     primer_bind     complement(2240..2258)
                     /label=LC693
                     /note="pLC82_BB_R"
     primer_bind     complement(2240..2258)
                     /label=ACV4
                     /note="BB_pZS25_pTet_RecET_Rv"
     primer_bind     complement(2240..2257)
                     /label=LC708
                     /note="pACV2_R3"
     primer_bind     2255..2278
                     /label=LC524_Ter_R
     primer_bind     2293..2310
                     /label=oGR335
                     /note="vector_pRG13A_R"
     primer_bind     complement(2295..2313)
                     /label=LC534
                     /note="pIT_R1"
     primer_bind     complement(2295..2313)
                     /label=LC193
                     /note="pZA31BB_R"
     primer_bind     complement(2297..2359)
                     /label=LC508
                     /note="sgPB_R1"
     primer_bind     2298..2317
                     /label=SB17
                     /note="SB17_Intetrate49"
     primer_bind     2299..2363
                     /label=LC507
                     /note="sgPB_F1"
     primer_bind     complement(2299..2317)
                     /label=ES75
                     /note="BB_primer5"
     primer_bind     2299..2315
                     /label=LC287
                     /note="LacZ2mut1K_F"
     primer_bind     complement(2302..2324)
                     /label=LC915
                     /note="pLC108_BR"
     primer_bind     complement(2302..2323)
                     /label=LC34
                     /note="pCRISPR_BB_R"
     primer_bind     2306..2342
                     /label=LC39
                     /note="ELZ2_1K_F"
     primer_bind     complement(2306..2342)
                     /label=LC42
                     /note="ELZ2MutBB_R"
     primer_bind     complement(2306..2324)
                     /label=BG61
                     /note="pZA31_R"
     primer_bind     2306..2320
                     /label=ES72
                     /note="primer6_fow_pES4"
     primer_bind     2307..2363
                     /label=LC278
                     /note="sgRNA_F"
     primer_bind     complement(2316..2333)
                     /label=LC286_sgRNAbb_R
     primer_bind     complement(2343..2363)
                     /label=LC519
                     /note="Cas9_R1"
     primer_bind     2343..2363
                     /label=LC529_psgRNA_F3
     primer_bind     2343..2363
                     /label=LC410
                     /note="PC31attP_F"
     primer_bind     2343..2363
                     /label=LC408
                     /note="sgRNA_RR1"
     primer_bind     2343..2363
                     /label=LC406
                     /note="sgRNA_RR"
     primer_bind     2343..2363
                     /label=LC334
                     /note="sgPhiC31P3"
     primer_bind     2343..2363
                     /label=LC336
                     /note="sgPhi_1F"
     primer_bind     2343..2363
                     /label=LC280_sgRNAbb_F
     primer_bind     complement(2343..2362)
                     /label=LC319
                     /note="sgRNA_R2"
     primer_bind     complement(2343..2362)
                     /label=LC343
                     /note="pCasBB_R"
     primer_bind     complement(2343..2362)
                     /label=LC314_Cas9nbb_R
     primer_bind     2343..2362
                     /label=B545
                     /note="delta-cas9-F"
     primer_bind     2343..2361
                     /label=LC317_sgRNA_F1
     primer_bind     2343..2361
                     /label=LC315_sgRNA_F
     primer_bind     2343..2360
                     /label=LC935_pLC113_iF
     primer_bind     2343..2360
                     /label=LC517
                     /note="pLC2_Rev"
     primer_bind     2343..2360
                     /label=D024
                     /note="Spacer Seq"
     CDS             2343..2358
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="LGGD"
     primer_bind     2361..2420
                     /label=LC296
                     /note="LC_array_F"
     primer_bind     2370..2415
                     /label=LC250
                     /note="mcherry_F2"
     primer_bind     2383..2420
                     /label=LC497
                     /note="P1For_2"
     primer_bind     2384..2420
                     /label=LC498
                     /note="P1For_3"
     primer_bind     2384..2420
                     /label=LC496
                     /note="P1For_1"
     primer_bind     complement(2385..2406)
                     /label=potential primer reverse backbone
     primer_bind     2385..2406
                     /label=potential forward primer for library
     promoter        2386..2414
                     /label=Promoter (BBa_J23119)
                     /note="from BioBricks"
     -35_signal      2386..2391
                     /label=-35
     primer_bind     2387..2420
                     /label=LC633
                     /note="NGS_P1_F_8N"
     primer_bind     2387..2418
                     /label=LC715
                     /note="DB_seq_F1"
     primer_bind     complement(2388..2420)
                     /label=LC500
                     /note="index2primer"
     primer_bind     2388..2420
                     /label=LC495
                     /note="Read1_P"
     primer_bind     2389..2420
                     /label=LC632
                     /note="NGS_P1_F_7N"
     primer_bind     2390..2420
                     /label=LC413
                     /note="NGS_P1_F"
     primer_bind     2391..2420
                     /label=LC631
                     /note="NGS_P1_F_5N"
     primer_bind     2392..2420
                     /label=LC630
                     /note="NGS_P1_F_4N"
     primer_bind     2393..2420
                     /label=LC629
                     /note="NGS_P1_F_3N"
     primer_bind     2394..2467
                     /label=LC463
                     /note="pal_oligo"
     primer_bind     2394..2467
                     /label=LC462
                     /note="depG_oligo"
     primer_bind     2394..2467
                     /label=LC313
                     /note="mhpRsg"
     primer_bind     2394..2467
                     /label=LC312
                     /note="eamBsg"
     primer_bind     2394..2467
                     /label=LC311
                     /note="garDsg"
     primer_bind     2394..2467
                     /label=LC298
                     /note="LacZ1sg"
     primer_bind     2394..2467
                     /label=LC295
                     /note="sgRNAlib"
     primer_bind     complement(2394..2453)
                     /label=LC1030
                     /note="ACCCA_non1_5R"
     primer_bind     complement(2394..2453)
                     /label=LC1028
                     /note="ACCCA_non1_10R"
     primer_bind     complement(2394..2420)
                     /label=V74
                     /note="spacer-backbone-R"
     primer_bind     complement(2394..2420)
                     /label=LC294
                     /note="sgRNAbblib_R"
     primer_bind     2396..2418
                     /label=LC608_P1F_V4P3i3
     primer_bind     2396..2418
                     /label=LC607_P1F_V4P3i2
     primer_bind     2396..2418
                     /label=LC609_CustomR1V4P3
                     /note="Custom read 1 V4 primer-3"
     primer_bind     2396..2418
                     /label=P1 forward for Read 1 V4-3
     primer_bind     2396..2418
                     /label=LC606_P1F_V4P3i1
     primer_bind     complement(2397..2420)
                     /label=V93
                     /note="pAV4topAV6_R"
     -10_signal      2409..2414
                     /label=-10
     primer_bind     2417..2440
                     /label=ES99
                     /note="ctrole-F"
     primer_bind     2421..2467
                     /label=LC576
                     /note="pLtetBsaIsg"
     primer_bind     2421..2467
                     /label=LC384
                     /note="LC7_oligo"
     primer_bind     2421..2446
                     /label=LC860
                     /note="sgRNA_R2"
     primer_bind     complement(2421..2444)
                     /label=ES98
                     /note="ctrole-R"
     misc_feature    2421..2440
                     /label=Control spacer with 2 BsaI
     primer_bind     2439..2463
                     /label=V92
                     /note="pAV4topAV6_F"
     misc_RNA        2441..2523
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     primer_bind     complement(2441..2500)
                     /label=LC297
                     /note="LC_array_R"
     primer_bind     complement(2441..2474)
                     /label=LC638
                     /note="DBlib_P1_R_8N"
     primer_bind     complement(2441..2473)
                     /label=LC867
                     /note="DB_P1R_N6"
     primer_bind     complement(2441..2473)
                     /label=LC869
                     /note="DB_P1R_N8"
     primer_bind     complement(2441..2473)
                     /label=LC637
                     /note="DBlib_P1_R_7N"
     primer_bind     complement(2441..2472)
                     /label=LC868
                     /note="DB_P1R_N7"
     primer_bind     complement(2441..2471)
                     /label=LC866
                     /note="DB_P1R_N5"
     primer_bind     complement(2441..2471)
                     /label=LC615
                     /note="DBlib_P1_R"
     primer_bind     complement(2441..2470)
                     /label=LC636
                     /note="DBlib_P1_R_5N"
     primer_bind     complement(2441..2469)
                     /label=LC864
                     /note="DB_P1R_N3"
     primer_bind     complement(2441..2469)
                     /label=LC865
                     /note="DB_P1R_N4"
     primer_bind     complement(2441..2469)
                     /label=LC635
                     /note="DBlib_P1_R_4N"
     primer_bind     complement(2441..2468)
                     /label=LC716
                     /note="DB_seq_R1"
     primer_bind     complement(2441..2468)
                     /label=LC634
                     /note="DBlib_P1_R_3N"
     primer_bind     2441..2467
                     /label=V65
                     /note="spacer-scaffold-F"
     primer_bind     2441..2467
                     /label=LC293
                     /note="sgRNAbblib_F"
     primer_bind     complement(2441..2465)
                     /label=LC412
                     /note="sgRNA_R1"
     primer_bind     2441..2463
                     /label=LC1029
                     /note="ACCCA_non1_5F"
     primer_bind     2441..2463
                     /label=LC1027
                     /note="ACCCA_non1_10F"
     primer_bind     2441..2463
                     /label=B562
                     /note="sgRNA-kansp-F"
     primer_bind     2441..2463
                     /label=B569
                     /note="rep5m_sp-F"
     primer_bind     2459..2482
                     /label=potential primer for backbone
     primer_bind     complement(2460..2481)
                     /label=LC877
                     /note="tracrRNA"
     primer_bind     complement(2493..2511)
                     /label=potential primer for library
     primer_bind     complement(2507..2528)
                     /label=LC344
                     /note="sgphiattB_R"
     primer_bind     complement(2507..2528)
                     /label=LC335
                     /note="sgPhiC31P3R"
     primer_bind     complement(2507..2528)
                     /label=LC271
                     /note="Cas9sgE2_R"
     primer_bind     complement(2507..2525)
                     /label=LC381
                     /note="Tracr_F2"
     primer_bind     complement(2507..2524)
                     /label=V66
                     /note="prefix-scaffold-R"
     primer_bind     complement(2508..36)
                     /label=LC279
                     /note="sgRNA_R"
     primer_bind     complement(2508..5)
                     /label=LC699
                     /note="pLC86insert_R"
     primer_bind     complement(2508..2528)
                     /label=LC405
                     /note="sgRNA_RF"
     gene            2524..2528
                     /gene="lacI"
                     /gene_synonym="ECK0342; JW0336"
                     /locus_tag="b0345"
                     /label=lacI
                     /db_xref="EcoGene:EG10525"
                     /db_xref="GeneID:945007"
     CDS             2524..2528
                     /codon_start=1
                     /transl_table=11
                     /gene="lacI"
                     /gene_synonym="ECK0342; JW0336"
                     /locus_tag="b0345"
                     /product="lactose-inducible lac operon transcriptional 
                     repressor"
                     /function="regulator; Degradation of small molecules: 
                     Carbon compounds"
                     /label=lacI
                     /note="transcriptional repressor of the lac operon"
                     /db_xref="GI:49176012"
                     /db_xref="ASAP:ABE-0001189"
                     /db_xref="UniProtKB/Swiss-Prot:P03023"
                     /db_xref="EcoGene:EG10525"
                     /db_xref="GeneID:945007"
                     /experiment="N-terminus verified by Edman degradation: PMID
                     1107032,4554534,4557395,4571224"
                     /protein_id="NP_414879.3"
                     /translation="V"
ORIGIN
        1 tgcagcgcga tcgtaatcag gatcccatgg tacgcgtgct agaggcatca aataaaacga
       61 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      121 ctgagtagga caaatccgcc gccctagacc ctccacgcac gttgtgatat gtagatgata
      181 atcattatca ctttacgggt cctttccggt gatccgacag gttacggggc ggcgacctcg
      241 cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct tcttcgtcat
      301 aacttaatgt ttttatttaa aataccctct gaaaagaaag gaaacgacag gtgctgaaag
      361 cgaggctttt tggggcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      421 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      481 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      541 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      601 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      661 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
      721 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      781 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      841 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      901 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      961 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1021 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1081 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1141 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct
     1201 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1261 aggtcattac tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc
     1321 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     1381 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     1441 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     1501 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     1561 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     1621 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     1681 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     1741 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     1801 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     1861 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     1921 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     1981 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     2041 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     2101 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct
     2161 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     2221 cttaccagag ggcgccccag ctggcaattc cgacgtctaa gaaaccatta ttatcatgac
     2281 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcgggccc attaagttct
     2341 gtgctaggag gtgactgaag tatattttag gaattctaaa gatctttgac agctagctca
     2401 gtcctaggta taatactagt tgagaccagt ctaggtctcg gttttagagc tagaaatagc
     2461 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     2521 tttggtag
//
