LOCUS       Exported                9724 bp ds-DNA     circular SYN 21-JAN-2015
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    F39 pLV-CMV-MCS-Ires-Puro Sequenced 98%
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="/dnas_title=RFP-MAD2 F39 pLV-CMV-MCS-Ires-Puro 
                     Sequenced"
     CDS             39..638
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=pac from Streptomyces alboniger
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="/dnas_title=pac from Streptomyces alboniger"
     misc_feature    906..1140
                     /label=This part sequencing failed
                     /note="This part sequencing failed"
     LTR             1530..1763
                     /label=3' LTR (Delta-U3)
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1835..1956
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1996..2131
                     /label=SV40 ori
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     misc_feature    2129..2148
                     /label=Probably GgCctaggGACGTACCCa
                     /note="Probably GgCctaggGACGTACCCa"
     rep_origin      2341..2796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2822..2926
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
                     /note="/dnas_title=bla"
     CDS             2927..3787
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="/dnas_title=bla"
     rep_origin      3958..4546
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4834..4855
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4870..4900
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4908..4924
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        5013..5241
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5242..5422
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5469..5594
                     /label=HIV-1 Psi
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6087..6320
                     /label=RRE
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6811..6928
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6951..7254
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7255..7458
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    7613..7615
                     /label=stop
                     /note="stop"
     misc_feature    7620..8330
                     /label=tagRFP
                     /note="/dnas_title=tagRFP"
                     /note="/vntifkey=21"
     misc_difference 8376..8381
                     /label=old XbaI/AvrII site
                     /note="/dnas_title=old XbaI/AvrII site"
                     /note="/vntifkey=85"
     misc_feature    8382..8999
                     /label=Mad2
                     /note="/dnas_title=Mad2"
                     /note="/vntifkey=21"
     misc_feature    9002..9004
                     /label=stop
                     /note="stop"
     misc_feature    9007..9009
                     /label=stop
                     /note="stop"
     misc_feature    9031..9033
                     /label=stop
                     /note="stop"
     misc_feature    9070..9072
                     /label=stop
                     /note="stop"
ORIGIN
        1 acgatgataa tatggatatg gccacaacgg tcgccaccat gaccgagtac aagcccacgg
       61 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
      121 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
      181 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
      241 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
      301 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
      361 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
      421 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
      481 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
      541 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
      601 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
      661 gcccgaccga aaggagcgca cgaccccatg catgtattca atctaagcag gctttcactt
      721 tctcgccaac ttacaaggcc tttctgtgta aacaatacct gaacctttac cccgttgccc
      781 ggcaacggcc aggtctgtgc caagtgtttg ctgacgcaac ccccactggc tggggcttgg
      841 tcatgggcca tcagcgcatg cgtggaacct tttcggctcc tctgccgatc catactgcgg
      901 aactcctagc cgcttgtttt gctcgcagca ggtctggagc aaacattatc gggactgata
      961 actctgttgt cctatcccgc aaatatacat cgtttccatg gctgctaggc tgtgctgcca
     1021 actggatcct gcgcgggacg tcctttgttt acgtcccgtc ggcgctgaat cctgcggacg
     1081 acccttctcg gggtcgcttg ggactctctc gtccccttct ccgtctgccg ttccgaccga
     1141 ccacggggcg cacctctctt tacgcggact ccccgtctgt gccttctcat ctgccggacc
     1201 gtgtgcactt cgcttcacct ctgcacgtcg catggagacc accgtgaacg cccaccaaat
     1261 attgcccaag gtcttacata agaggactct tggactctca gcaatgtcaa cgaccgacct
     1321 tgaggcatac ttcaaagact gtttgtttaa agactgggag gagttggggg aggagattag
     1381 gttaaaggtc tttgtactag gaggctgtag gcataaattg gtctgcgcac cagcaccatg
     1441 gcgcaatcac tagagcgggg tacctttaag accaatgact tacaaggcag ctgtagatct
     1501 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     1561 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     1621 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1681 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1741 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     1801 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     1861 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1921 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     1981 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2041 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2101 cagaagtagt gaggaggctt ttttggaggc ccgctaggga cgtacccmat tcgccctata
     2161 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2221 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     2341 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2401 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2461 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2521 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2581 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2641 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2701 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2761 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3001 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3061 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3121 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3181 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3241 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3301 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3361 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3421 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3481 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3541 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3601 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3661 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3721 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3781 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3841 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3901 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4621 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4681 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4741 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4801 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4861 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4921 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     4981 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     5041 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     5101 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     5161 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     5221 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     5281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5401 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     5461 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     5521 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     5581 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     5641 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     5701 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     5761 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     5821 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     5881 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     5941 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6001 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6061 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6121 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6181 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6241 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     6301 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6361 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6421 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6481 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6541 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     6601 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     6661 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     6721 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6781 cgattagtga acggatctcg acggtatcgg ttttaaaaga aaagggggga ttggggggta
     6841 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     6901 aaaacaaatt acaaaaattc aaaattttat cgataagctt gggagttccg cgttacataa
     6961 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7021 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     7081 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     7141 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     7201 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     7261 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     7321 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     7381 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     7441 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     7501 tgttttgacc tccatagaag acaccgactc tagctagagg atcccccggg ctgcaggaat
     7561 tcggcgcgcc acgcgtaccg atatccagca cagtggcggc cgctcgagcc tctagagcca
     7621 tggtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg
     7681 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
     7741 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
     7801 tcctggctac cagcttcatg tacggcagca gaaccttcat caaccacacc cagggcatcc
     7861 ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg
     7921 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct
     7981 acaacgtcaa gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa
     8041 cactcggctg ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctggaaggca
     8101 gaagcgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttcaagacca
     8161 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggacc
     8221 acagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg
     8281 ctgtggccag atactgcgac ctccctagca aactggggca caaacttaat gaattctgca
     8341 gatatccatc acactggcgg ccgctcgagc atgcatctag gatggcgctg cagctctccc
     8401 gggagcaggg aatcaccctg cgcgggagcg ccgaaatcgt ggccgagttc ttctcattcg
     8461 gcatcaacag cattttatat cagcgtggca tatatccatc tgaaaccttt actcgagtgc
     8521 agaaatacgg actcaccttg cttgtaacta ctgatcttga gctcataaaa tacctaaata
     8581 atgtggtgga acaactgaaa gattggttat acaagtgttc agttcagaaa ctggttgtag
     8641 ttatctcaaa tattgaaagt ggtgaggtcc tggaaagatg gcagtttgat attgagtgtg
     8701 acaagactgc aaaagatgac agtgcaccca gagaaaagtc tcagaaagct atccaggatg
     8761 aaatccgttc agtgatcaga cagatcacag ctacggtgac atttctgcca ctgttggaag
     8821 tttcttgttc atttgatctg ctgatttata cagacaaaga tttggttgta cctgaaaaat
     8881 gggaagaatc aggaccacag tttattacca attctgagga agtccgcctt cgttcattta
     8941 ctactacaat ccacaaagta aatagcatgg tggcctacaa aattcctgtc aatgactgag
     9001 ctagcctagt gccatttgtt cagtggttcg tagggctttc ccccactgtt tggctttcag
     9061 ttatatggat gatgtggtat tgggggccaa gtctgtacag catcttgagt ccctttttac
     9121 cgctgttacc aattttcttt tgtctttggg taaacccggg aattccgccc ctctcccccc
     9181 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     9241 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     9301 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     9361 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     9421 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     9481 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     9541 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     9601 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     9661 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     9721 aaac
//
